LisH domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9AP35_LEIMU

TriTrypDb:

LmxM.13.0010

Length:

650

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 9
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005840	ribosome	5	2
GO:0016604	nuclear body	2	2
GO:0016607	nuclear speck	3	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

E9AP35

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP35

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	5
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	5
GO:0000398	mRNA splicing, via spliceosome	8	5
GO:0006139	nucleobase-containing compound metabolic process	3	5
GO:0006396	RNA processing	6	5
GO:0006397	mRNA processing	7	5
GO:0006725	cellular aromatic compound metabolic process	3	5
GO:0006807	nitrogen compound metabolic process	2	5
GO:0008152	metabolic process	1	5
GO:0008380	RNA splicing	7	5
GO:0009987	cellular process	1	5
GO:0016070	RNA metabolic process	5	5
GO:0016071	mRNA metabolic process	6	5
GO:0034641	cellular nitrogen compound metabolic process	3	5
GO:0043170	macromolecule metabolic process	3	5
GO:0044237	cellular metabolic process	2	5
GO:0044238	primary metabolic process	2	5
GO:0046483	heterocycle metabolic process	3	5
GO:0071704	organic substance metabolic process	2	5
GO:0090304	nucleic acid metabolic process	4	5
GO:1901360	organic cyclic compound metabolic process	3	5

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	390	394	PF00656	0.326
CLV_NRD_NRD_1	257	259	PF00675	0.621
CLV_NRD_NRD_1	369	371	PF00675	0.571
CLV_NRD_NRD_1	75	77	PF00675	0.452
CLV_PCSK_KEX2_1	257	259	PF00082	0.605
CLV_PCSK_KEX2_1	369	371	PF00082	0.543
CLV_PCSK_SKI1_1	104	108	PF00082	0.635
CLV_PCSK_SKI1_1	146	150	PF00082	0.457
CLV_PCSK_SKI1_1	153	157	PF00082	0.409
CLV_PCSK_SKI1_1	20	24	PF00082	0.735
CLV_PCSK_SKI1_1	236	240	PF00082	0.566
CLV_PCSK_SKI1_1	96	100	PF00082	0.571
DEG_APCC_DBOX_1	515	523	PF00400	0.536
DEG_APCC_DBOX_1	75	83	PF00400	0.431
DEG_Nend_UBRbox_3	1	3	PF02207	0.755
DEG_ODPH_VHL_1	297	308	PF01847	0.613
DOC_CYCLIN_yCln2_LP_2	267	273	PF00134	0.603
DOC_CYCLIN_yCln2_LP_2	491	497	PF00134	0.552
DOC_MAPK_MEF2A_6	38	46	PF00069	0.479
DOC_PP1_RVXF_1	372	379	PF00149	0.507
DOC_PP2B_LxvP_1	267	270	PF13499	0.664
DOC_PP2B_LxvP_1	330	333	PF13499	0.520
DOC_PP4_MxPP_1	225	228	PF00568	0.561
DOC_USP7_MATH_1	113	117	PF00917	0.482
DOC_USP7_MATH_1	180	184	PF00917	0.592
DOC_USP7_MATH_1	343	347	PF00917	0.627
DOC_USP7_MATH_1	94	98	PF00917	0.501
DOC_USP7_UBL2_3	104	108	PF12436	0.659
DOC_USP7_UBL2_3	96	100	PF12436	0.568
DOC_WW_Pin1_4	114	119	PF00397	0.657
DOC_WW_Pin1_4	21	26	PF00397	0.658
DOC_WW_Pin1_4	490	495	PF00397	0.444
DOC_WW_Pin1_4	502	507	PF00397	0.342
DOC_WW_Pin1_4	543	548	PF00397	0.615
LIG_14-3-3_CanoR_1	146	151	PF00244	0.527
LIG_14-3-3_CanoR_1	258	267	PF00244	0.585
LIG_14-3-3_CanoR_1	291	299	PF00244	0.626
LIG_14-3-3_CanoR_1	463	469	PF00244	0.482
LIG_14-3-3_CanoR_1	545	551	PF00244	0.477
LIG_14-3-3_CanoR_1	617	625	PF00244	0.471
LIG_14-3-3_CanoR_1	76	80	PF00244	0.476
LIG_Actin_RPEL_3	31	50	PF02755	0.592
LIG_BIR_III_4	14	18	PF00653	0.785
LIG_BRCT_BRCA1_1	124	128	PF00533	0.488
LIG_BRCT_BRCA1_1	506	510	PF00533	0.548
LIG_CSL_BTD_1	330	333	PF09270	0.489
LIG_CtBP_PxDLS_1	228	232	PF00389	0.672
LIG_EH1_1	453	461	PF00400	0.502
LIG_eIF4E_1	454	460	PF01652	0.480
LIG_FHA_1	101	107	PF00498	0.548
LIG_FHA_1	169	175	PF00498	0.556
LIG_FHA_1	191	197	PF00498	0.510
LIG_FHA_1	230	236	PF00498	0.668
LIG_FHA_1	24	30	PF00498	0.746
LIG_FHA_1	324	330	PF00498	0.497
LIG_FHA_1	332	338	PF00498	0.561
LIG_FHA_1	371	377	PF00498	0.516
LIG_FHA_1	381	387	PF00498	0.463
LIG_FHA_1	422	428	PF00498	0.554
LIG_FHA_1	432	438	PF00498	0.470
LIG_FHA_1	474	480	PF00498	0.397
LIG_FHA_1	482	488	PF00498	0.405
LIG_FHA_1	507	513	PF00498	0.453
LIG_FHA_1	525	531	PF00498	0.278
LIG_FHA_1	576	582	PF00498	0.562
LIG_FHA_2	176	182	PF00498	0.537
LIG_FHA_2	35	41	PF00498	0.539
LIG_FHA_2	534	540	PF00498	0.594
LIG_FHA_2	551	557	PF00498	0.445
LIG_LIR_Gen_1	147	155	PF02991	0.548
LIG_LIR_Gen_1	165	174	PF02991	0.341
LIG_LIR_Gen_1	242	253	PF02991	0.509
LIG_LIR_Gen_1	278	289	PF02991	0.572
LIG_LIR_Gen_1	292	302	PF02991	0.500
LIG_LIR_Gen_1	444	454	PF02991	0.491
LIG_LIR_Nem_3	147	151	PF02991	0.525
LIG_LIR_Nem_3	165	170	PF02991	0.329
LIG_LIR_Nem_3	183	189	PF02991	0.278
LIG_LIR_Nem_3	211	217	PF02991	0.567
LIG_LIR_Nem_3	242	248	PF02991	0.495
LIG_LIR_Nem_3	278	284	PF02991	0.566
LIG_LIR_Nem_3	292	297	PF02991	0.596
LIG_LIR_Nem_3	444	450	PF02991	0.494
LIG_LIR_Nem_3	452	457	PF02991	0.500
LIG_NRBOX	169	175	PF00104	0.417
LIG_PCNA_yPIPBox_3	453	463	PF02747	0.445
LIG_Pex14_2	239	243	PF04695	0.609
LIG_REV1ctd_RIR_1	148	156	PF16727	0.430
LIG_SH2_CRK	454	458	PF00017	0.484
LIG_SH2_GRB2like	214	217	PF00017	0.687
LIG_SH2_NCK_1	245	249	PF00017	0.560
LIG_SH2_STAP1	447	451	PF00017	0.577
LIG_SH2_STAP1	609	613	PF00017	0.652
LIG_SH2_STAT3	596	599	PF00017	0.769
LIG_SH2_STAT3	609	612	PF00017	0.488
LIG_SH2_STAT5	172	175	PF00017	0.445
LIG_SH2_STAT5	217	220	PF00017	0.588
LIG_SH2_STAT5	468	471	PF00017	0.375
LIG_SH2_STAT5	567	570	PF00017	0.419
LIG_SH2_STAT5	596	599	PF00017	0.722
LIG_SH2_STAT5	74	77	PF00017	0.568
LIG_SH3_2	580	585	PF14604	0.644
LIG_SH3_3	220	226	PF00018	0.736
LIG_SH3_3	330	336	PF00018	0.570
LIG_SH3_3	339	345	PF00018	0.664
LIG_SH3_3	577	583	PF00018	0.722
LIG_SUMO_SIM_par_1	227	232	PF11976	0.677
LIG_SUMO_SIM_par_1	26	33	PF11976	0.646
LIG_SUMO_SIM_par_1	382	390	PF11976	0.256
LIG_SUMO_SIM_par_1	423	428	PF11976	0.525
LIG_SUMO_SIM_par_1	478	484	PF11976	0.437
LIG_SUMO_SIM_par_1	635	644	PF11976	0.522
LIG_TRAF2_1	85	88	PF00917	0.546
MOD_CK1_1	292	298	PF00069	0.673
MOD_CK1_1	380	386	PF00069	0.529
MOD_CK1_1	387	393	PF00069	0.564
MOD_CK1_1	431	437	PF00069	0.495
MOD_CK1_1	490	496	PF00069	0.493
MOD_CK1_1	505	511	PF00069	0.242
MOD_CK1_1	546	552	PF00069	0.580
MOD_CK1_1	598	604	PF00069	0.633
MOD_CK1_1	90	96	PF00069	0.594
MOD_CK2_1	175	181	PF00069	0.545
MOD_CK2_1	34	40	PF00069	0.547
MOD_CK2_1	533	539	PF00069	0.566
MOD_CK2_1	550	556	PF00069	0.603
MOD_CK2_1	629	635	PF00069	0.538
MOD_CK2_1	86	92	PF00069	0.559
MOD_GlcNHglycan	108	111	PF01048	0.509
MOD_GlcNHglycan	267	270	PF01048	0.664
MOD_GlcNHglycan	285	289	PF01048	0.579
MOD_GlcNHglycan	294	297	PF01048	0.615
MOD_GlcNHglycan	317	320	PF01048	0.559
MOD_GlcNHglycan	337	340	PF01048	0.537
MOD_GlcNHglycan	393	396	PF01048	0.570
MOD_GlcNHglycan	558	561	PF01048	0.467
MOD_GlcNHglycan	56	59	PF01048	0.449
MOD_GlcNHglycan	591	594	PF01048	0.700
MOD_GlcNHglycan	646	649	PF01048	0.639
MOD_GlcNHglycan	8	11	PF01048	0.728
MOD_GlcNHglycan	92	95	PF01048	0.539
MOD_GSK3_1	100	107	PF00069	0.463
MOD_GSK3_1	168	175	PF00069	0.499
MOD_GSK3_1	204	211	PF00069	0.521
MOD_GSK3_1	259	266	PF00069	0.628
MOD_GSK3_1	280	287	PF00069	0.595
MOD_GSK3_1	30	37	PF00069	0.599
MOD_GSK3_1	331	338	PF00069	0.559
MOD_GSK3_1	380	387	PF00069	0.351
MOD_GSK3_1	421	428	PF00069	0.537
MOD_GSK3_1	469	476	PF00069	0.482
MOD_GSK3_1	481	488	PF00069	0.428
MOD_GSK3_1	498	505	PF00069	0.282
MOD_GSK3_1	535	542	PF00069	0.585
MOD_GSK3_1	546	553	PF00069	0.459
MOD_GSK3_1	625	632	PF00069	0.596
MOD_GSK3_1	640	647	PF00069	0.481
MOD_GSK3_1	86	93	PF00069	0.547
MOD_N-GLC_1	370	375	PF02516	0.509
MOD_N-GLC_1	461	466	PF02516	0.439
MOD_N-GLC_1	539	544	PF02516	0.612
MOD_N-GLC_2	216	218	PF02516	0.701
MOD_N-GLC_2	222	224	PF02516	0.631
MOD_NEK2_1	122	127	PF00069	0.435
MOD_NEK2_1	151	156	PF00069	0.448
MOD_NEK2_1	158	163	PF00069	0.404
MOD_NEK2_1	239	244	PF00069	0.501
MOD_NEK2_1	259	264	PF00069	0.621
MOD_NEK2_1	289	294	PF00069	0.559
MOD_NEK2_1	315	320	PF00069	0.561
MOD_NEK2_1	384	389	PF00069	0.376
MOD_NEK2_1	391	396	PF00069	0.596
MOD_NEK2_1	425	430	PF00069	0.546
MOD_NEK2_1	461	466	PF00069	0.520
MOD_NEK2_1	487	492	PF00069	0.433
MOD_NEK2_1	511	516	PF00069	0.400
MOD_NEK2_1	54	59	PF00069	0.440
MOD_NEK2_1	595	600	PF00069	0.680
MOD_NEK2_1	641	646	PF00069	0.611
MOD_NEK2_1	75	80	PF00069	0.547
MOD_NEK2_2	172	177	PF00069	0.428
MOD_NEK2_2	234	239	PF00069	0.547
MOD_NEK2_2	94	99	PF00069	0.558
MOD_PIKK_1	204	210	PF00454	0.489
MOD_PIKK_1	595	601	PF00454	0.739
MOD_PKA_2	260	266	PF00069	0.633
MOD_PKA_2	290	296	PF00069	0.596
MOD_PKA_2	533	539	PF00069	0.546
MOD_PKA_2	75	81	PF00069	0.480
MOD_Plk_1	158	164	PF00069	0.567
MOD_Plk_1	284	290	PF00069	0.579
MOD_Plk_1	86	92	PF00069	0.594
MOD_Plk_2-3	87	93	PF00069	0.599
MOD_Plk_4	168	174	PF00069	0.554
MOD_Plk_4	380	386	PF00069	0.437
MOD_Plk_4	387	393	PF00069	0.480
MOD_Plk_4	455	461	PF00069	0.484
MOD_Plk_4	464	470	PF00069	0.513
MOD_Plk_4	487	493	PF00069	0.539
MOD_Plk_4	506	512	PF00069	0.393
MOD_ProDKin_1	114	120	PF00069	0.650
MOD_ProDKin_1	21	27	PF00069	0.656
MOD_ProDKin_1	490	496	PF00069	0.455
MOD_ProDKin_1	502	508	PF00069	0.341
MOD_ProDKin_1	543	549	PF00069	0.611
MOD_SUMO_for_1	623	626	PF00179	0.596
TRG_ENDOCYTIC_2	135	138	PF00928	0.525
TRG_ENDOCYTIC_2	245	248	PF00928	0.532
TRG_ENDOCYTIC_2	447	450	PF00928	0.552
TRG_ENDOCYTIC_2	454	457	PF00928	0.502
TRG_ER_diArg_1	256	258	PF00400	0.617
TRG_ER_diArg_1	368	370	PF00400	0.559
TRG_ER_diArg_1	571	574	PF00400	0.555
TRG_Pf-PMV_PEXEL_1	236	240	PF00026	0.607
TRG_Pf-PMV_PEXEL_1	84	88	PF00026	0.496

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2V3	Leptomonas seymouri	70%	97%
A0A1X0NHC6	Trypanosomatidae	41%	98%
A0A3R7RLK0	Trypanosoma rangeli	43%	98%
A0A3S7WS82	Leishmania donovani	95%	100%
A4H709	Leishmania braziliensis	89%	100%
A4HVD7	Leishmania infantum	95%	100%
C9ZTR0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	87%
Q4QGF3	Leishmania major	93%	100%
V5BXX9	Trypanosoma cruzi	42%	98%