Putative surface antigen protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

surface antigen protein, putative

Species:

Leishmania mexicana

UniProt:

E9AP08_LEIMU

TriTrypDb:

LmxM.12.0990

Length:

420

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 150

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 14

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 59, no: 8
NetGPI	no	yes: 0, no: 67

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	68
GO:0042995	cell projection	2	68
GO:0043226	organelle	2	68
GO:0043227	membrane-bounded organelle	3	68
GO:0110165	cellular anatomical entity	1	68
GO:0120025	plasma membrane bounded cell projection	3	68
GO:0016020	membrane	2	26
GO:0005886	plasma membrane	3	5

Expansion

Sequence features

E9AP08

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP08

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004672	protein kinase activity	3	3
GO:0004674	protein serine/threonine kinase activity	4	3
GO:0016301	kinase activity	4	3
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	6	8	PF00675	0.805
CLV_PCSK_KEX2_1	6	8	PF00082	0.805
CLV_PCSK_SKI1_1	182	186	PF00082	0.511
CLV_PCSK_SKI1_1	255	259	PF00082	0.507
CLV_PCSK_SKI1_1	7	11	PF00082	0.849
DEG_SPOP_SBC_1	305	309	PF00917	0.612
DEG_SPOP_SBC_1	312	316	PF00917	0.574
DEG_SPOP_SBC_1	323	327	PF00917	0.578
DEG_SPOP_SBC_1	337	341	PF00917	0.691
DEG_SPOP_SBC_1	345	349	PF00917	0.665
DOC_CYCLIN_yCln2_LP_2	10	16	PF00134	0.486
DOC_MAPK_gen_1	275	284	PF00069	0.361
DOC_MAPK_HePTP_8	102	114	PF00069	0.279
DOC_MAPK_MEF2A_6	105	114	PF00069	0.276
DOC_MAPK_MEF2A_6	275	284	PF00069	0.405
DOC_PP1_RVXF_1	253	259	PF00149	0.296
DOC_PP2B_LxvP_1	10	13	PF13499	0.490
DOC_USP7_MATH_1	33	37	PF00917	0.381
DOC_USP7_MATH_1	344	348	PF00917	0.518
DOC_USP7_MATH_1	396	400	PF00917	0.579
DOC_WW_Pin1_4	299	304	PF00397	0.459
DOC_WW_Pin1_4	317	322	PF00397	0.540
DOC_WW_Pin1_4	340	345	PF00397	0.585
DOC_WW_Pin1_4	346	351	PF00397	0.550
LIG_14-3-3_CanoR_1	214	222	PF00244	0.316
LIG_Actin_WH2_2	153	168	PF00022	0.311
LIG_Actin_WH2_2	201	216	PF00022	0.288
LIG_BIR_II_1	1	5	PF00653	0.664
LIG_BRCT_BRCA1_1	70	74	PF00533	0.442
LIG_Clathr_ClatBox_1	186	190	PF01394	0.276
LIG_FHA_1	145	151	PF00498	0.391
LIG_FHA_1	44	50	PF00498	0.479
LIG_FHA_1	90	96	PF00498	0.366
LIG_FHA_2	228	234	PF00498	0.322
LIG_FHA_2	281	287	PF00498	0.330
LIG_FHA_2	63	69	PF00498	0.487
LIG_FHA_2	92	98	PF00498	0.415
LIG_LIR_Gen_1	128	136	PF02991	0.422
LIG_LIR_Gen_1	249	258	PF02991	0.412
LIG_LIR_Gen_1	71	78	PF02991	0.352
LIG_LIR_LC3C_4	278	282	PF02991	0.326
LIG_LIR_Nem_3	103	107	PF02991	0.465
LIG_LIR_Nem_3	128	132	PF02991	0.347
LIG_LIR_Nem_3	192	198	PF02991	0.402
LIG_LIR_Nem_3	238	242	PF02991	0.387
LIG_LIR_Nem_3	71	75	PF02991	0.461
LIG_PDZ_Class_2	415	420	PF00595	0.568
LIG_PTAP_UEV_1	320	325	PF05743	0.475
LIG_PTAP_UEV_1	334	339	PF05743	0.478
LIG_SH2_CRK	239	243	PF00017	0.301
LIG_SH2_STAT5	239	242	PF00017	0.390
LIG_SH2_STAT5	91	94	PF00017	0.443
LIG_SH3_3	318	324	PF00018	0.565
LIG_SH3_3	332	338	PF00018	0.576
LIG_SH3_3	93	99	PF00018	0.473
LIG_SUMO_SIM_anti_2	106	111	PF11976	0.273
LIG_SUMO_SIM_anti_2	46	51	PF11976	0.360
LIG_SUMO_SIM_par_1	280	286	PF11976	0.363
LIG_TRAF2_1	283	286	PF00917	0.317
LIG_TYR_ITIM	102	107	PF00017	0.505
LIG_TYR_ITIM	237	242	PF00017	0.294
LIG_UBA3_1	135	142	PF00899	0.284
LIG_UBA3_1	186	193	PF00899	0.278
MOD_CK1_1	128	134	PF00069	0.442
MOD_CK1_1	155	161	PF00069	0.495
MOD_CK1_1	203	209	PF00069	0.425
MOD_CK1_1	227	233	PF00069	0.438
MOD_CK1_1	348	354	PF00069	0.599
MOD_CK1_1	36	42	PF00069	0.497
MOD_CK1_1	80	86	PF00069	0.462
MOD_CK2_1	227	233	PF00069	0.430
MOD_CK2_1	280	286	PF00069	0.313
MOD_CK2_1	351	357	PF00069	0.583
MOD_CK2_1	91	97	PF00069	0.407
MOD_Cter_Amidation	60	63	PF01082	0.507
MOD_GlcNHglycan	114	117	PF01048	0.588
MOD_GlcNHglycan	121	124	PF01048	0.595
MOD_GlcNHglycan	170	173	PF01048	0.547
MOD_GlcNHglycan	218	221	PF01048	0.636
MOD_GlcNHglycan	226	229	PF01048	0.598
MOD_GlcNHglycan	230	233	PF01048	0.599
MOD_GlcNHglycan	242	245	PF01048	0.695
MOD_GlcNHglycan	249	253	PF01048	0.621
MOD_GlcNHglycan	25	28	PF01048	0.832
MOD_GlcNHglycan	261	264	PF01048	0.570
MOD_GlcNHglycan	266	269	PF01048	0.533
MOD_GlcNHglycan	353	356	PF01048	0.655
MOD_GlcNHglycan	367	370	PF01048	0.695
MOD_GlcNHglycan	398	401	PF01048	0.327
MOD_GlcNHglycan	79	82	PF01048	0.643
MOD_GlcNHglycan	99	102	PF01048	0.657
MOD_GSK3_1	121	128	PF00069	0.455
MOD_GSK3_1	130	137	PF00069	0.411
MOD_GSK3_1	140	147	PF00069	0.354
MOD_GSK3_1	152	159	PF00069	0.426
MOD_GSK3_1	200	207	PF00069	0.448
MOD_GSK3_1	224	231	PF00069	0.418
MOD_GSK3_1	307	314	PF00069	0.536
MOD_GSK3_1	315	322	PF00069	0.532
MOD_GSK3_1	32	39	PF00069	0.635
MOD_GSK3_1	323	330	PF00069	0.517
MOD_GSK3_1	333	340	PF00069	0.572
MOD_GSK3_1	344	351	PF00069	0.540
MOD_GSK3_1	379	386	PF00069	0.472
MOD_GSK3_1	64	71	PF00069	0.420
MOD_GSK3_1	73	80	PF00069	0.449
MOD_N-GLC_1	112	117	PF02516	0.511
MOD_N-GLC_2	297	299	PF02516	0.726
MOD_NEK2_1	112	117	PF00069	0.396
MOD_NEK2_1	121	126	PF00069	0.409
MOD_NEK2_1	165	170	PF00069	0.501
MOD_NEK2_1	189	194	PF00069	0.395
MOD_NEK2_1	21	26	PF00069	0.626
MOD_NEK2_1	213	218	PF00069	0.398
MOD_NEK2_1	259	264	PF00069	0.322
MOD_OFUCOSY	70	77	PF10250	0.542
MOD_PKA_1	62	68	PF00069	0.340
MOD_PKA_2	165	171	PF00069	0.495
MOD_PKA_2	213	219	PF00069	0.333
MOD_Plk_1	112	118	PF00069	0.444
MOD_Plk_1	248	254	PF00069	0.510
MOD_Plk_1	383	389	PF00069	0.390
MOD_Plk_2-3	377	383	PF00069	0.340
MOD_Plk_4	131	137	PF00069	0.402
MOD_Plk_4	182	188	PF00069	0.312
MOD_Plk_4	45	51	PF00069	0.294
MOD_Plk_4	80	86	PF00069	0.350
MOD_ProDKin_1	299	305	PF00069	0.466
MOD_ProDKin_1	317	323	PF00069	0.539
MOD_ProDKin_1	340	346	PF00069	0.580
MOD_ProDKin_1	348	354	PF00069	0.524
MOD_SUMO_rev_2	286	296	PF00179	0.373
MOD_SUMO_rev_2	377	386	PF00179	0.360
MOD_SUMO_rev_2	97	107	PF00179	0.311
TRG_ENDOCYTIC_2	104	107	PF00928	0.445
TRG_ENDOCYTIC_2	239	242	PF00928	0.444
TRG_ER_diArg_1	5	7	PF00400	0.747
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.562

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I121	Leptomonas seymouri	28%	84%
A0A0S4ITN5	Bodo saltans	25%	100%
A0A0S4JB17	Bodo saltans	27%	67%
A0A0S4JU20	Bodo saltans	35%	100%
A0A0S4KK37	Bodo saltans	28%	72%
A0A0S4KMS7	Bodo saltans	26%	100%
A0A3Q8I9A6	Leishmania donovani	61%	100%
A0A3Q8I9B4	Leishmania donovani	59%	68%
A0A3Q8I9D0	Leishmania donovani	59%	100%
A0A3Q8I9D9	Leishmania donovani	59%	67%
A0A3Q8IIJ9	Leishmania donovani	36%	100%
A0A3S5H6D6	Leishmania donovani	27%	100%
A0A3S5H6L9	Leishmania donovani	59%	73%
A0A3S7WP69	Leishmania donovani	25%	100%
A0A3S7WPB2	Leishmania donovani	22%	100%
A0A3S7X4J4	Leishmania donovani	36%	100%
A4H4D2	Leishmania braziliensis	26%	100%
A4H4G6	Leishmania braziliensis	23%	100%
A4H5P0	Leishmania braziliensis	27%	100%
A4HJC8	Leishmania braziliensis	32%	100%
A4HJX1	Leishmania braziliensis	38%	100%
A4HSL2	Leishmania infantum	25%	100%
A4HTX9	Leishmania infantum	27%	100%
A4HVB0	Leishmania infantum	54%	100%
A4I6S4	Leishmania infantum	36%	100%
D1GJ51	Leishmania infantum	52%	91%
E8NHG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	95%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9ACQ0	Leishmania major	25%	100%
E9AG65	Leishmania infantum	22%	100%
E9AGG5	Leishmania infantum	68%	100%
E9AGG7	Leishmania infantum	43%	67%
E9AGG9	Leishmania infantum	49%	78%
E9AGH0	Leishmania infantum	63%	100%
E9AKJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AKM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9AMQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9AP02	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	86%
E9AP03	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	93%
E9AP05	Leishmania mexicana (strain MHOM/GT/2001/U1103)	56%	82%
E9AP07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	87%
E9AVA1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	91%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
Q25331	Leishmania major	54%	89%
Q4Q6B6	Leishmania major	35%	100%
Q4Q6B7	Leishmania major	35%	100%
Q4Q6B8	Leishmania major	38%	79%
Q4QGI0	Leishmania major	51%	83%
Q4QGI2	Leishmania major	48%	75%
Q4QGI4	Leishmania major	49%	78%
Q4QGI6	Leishmania major	44%	77%
Q4QGJ0	Leishmania major	51%	67%
Q4QGJ4	Leishmania major	54%	89%
Q4QGJ6	Leishmania major	53%	73%
Q4QGJ7	Leishmania major	56%	89%
Q4QGK2	Leishmania major	50%	75%
Q4QGK6	Leishmania major	54%	89%
Q4QGK8	Leishmania major	50%	82%
Q4QGL2	Leishmania major	50%	82%
Q4QGL4	Leishmania major	55%	89%
Q4QGL5	Leishmania major	49%	77%
Q4QGL8	Leishmania major	58%	69%
Q4QHW6	Leishmania major	27%	100%
Q4QJB2	Leishmania major	26%	100%