Putative surface antigen protein 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

surface antigen protein 2, putative

Species:

Leishmania mexicana

UniProt:

E9AP03_LEIMU

TriTrypDb:

LmxM.12.0891

Length:

391

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 150

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 61, no: 10
NetGPI	no	yes: 0, no: 71

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	72
GO:0042995	cell projection	2	72
GO:0043226	organelle	2	72
GO:0043227	membrane-bounded organelle	3	72
GO:0110165	cellular anatomical entity	1	72
GO:0120025	plasma membrane bounded cell projection	3	72
GO:0016020	membrane	2	22
GO:0005886	plasma membrane	3	5

Expansion

Sequence features

E9AP03

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP03

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004672	protein kinase activity	3	3
GO:0004674	protein serine/threonine kinase activity	4	3
GO:0016301	kinase activity	4	3
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	6	8	PF00675	0.775
CLV_PCSK_KEX2_1	6	8	PF00082	0.775
CLV_PCSK_SKI1_1	182	186	PF00082	0.604
CLV_PCSK_SKI1_1	7	11	PF00082	0.769
DEG_SPOP_SBC_1	308	312	PF00917	0.592
DEG_SPOP_SBC_1	316	320	PF00917	0.576
DOC_CYCLIN_yCln2_LP_2	10	16	PF00134	0.435
DOC_MAPK_gen_1	276	284	PF00069	0.317
DOC_MAPK_HePTP_8	102	114	PF00069	0.251
DOC_MAPK_MEF2A_6	105	114	PF00069	0.249
DOC_MAPK_MEF2A_6	276	284	PF00069	0.451
DOC_PP2B_LxvP_1	10	13	PF13499	0.448
DOC_USP7_MATH_1	315	319	PF00917	0.571
DOC_USP7_MATH_1	33	37	PF00917	0.481
DOC_USP7_MATH_1	367	371	PF00917	0.299
DOC_WW_Pin1_4	311	316	PF00397	0.597
DOC_WW_Pin1_4	317	322	PF00397	0.598
LIG_14-3-3_CanoR_1	214	222	PF00244	0.304
LIG_14-3-3_CanoR_1	257	262	PF00244	0.339
LIG_Actin_WH2_2	153	168	PF00022	0.287
LIG_Actin_WH2_2	201	216	PF00022	0.267
LIG_BIR_II_1	1	5	PF00653	0.678
LIG_BRCT_BRCA1_1	70	74	PF00533	0.457
LIG_Clathr_ClatBox_1	186	190	PF01394	0.301
LIG_FHA_1	145	151	PF00498	0.411
LIG_FHA_1	44	50	PF00498	0.470
LIG_FHA_1	90	96	PF00498	0.371
LIG_FHA_2	228	234	PF00498	0.421
LIG_FHA_2	281	287	PF00498	0.473
LIG_FHA_2	299	305	PF00498	0.588
LIG_FHA_2	63	69	PF00498	0.452
LIG_FHA_2	92	98	PF00498	0.429
LIG_LIR_Gen_1	128	136	PF02991	0.371
LIG_LIR_Gen_1	249	258	PF02991	0.409
LIG_LIR_Gen_1	71	78	PF02991	0.323
LIG_LIR_Nem_3	103	107	PF02991	0.428
LIG_LIR_Nem_3	128	132	PF02991	0.347
LIG_LIR_Nem_3	192	198	PF02991	0.313
LIG_LIR_Nem_3	238	242	PF02991	0.377
LIG_LIR_Nem_3	71	75	PF02991	0.379
LIG_PDZ_Class_2	386	391	PF00595	0.306
LIG_PTAP_UEV_1	305	310	PF05743	0.433
LIG_SH2_CRK	239	243	PF00017	0.272
LIG_SH2_STAT5	239	242	PF00017	0.400
LIG_SH2_STAT5	91	94	PF00017	0.292
LIG_SH3_3	272	278	PF00018	0.364
LIG_SH3_3	303	309	PF00018	0.536
LIG_SH3_3	93	99	PF00018	0.437
LIG_SUMO_SIM_anti_2	106	111	PF11976	0.245
LIG_SUMO_SIM_anti_2	46	51	PF11976	0.329
LIG_SUMO_SIM_par_1	257	262	PF11976	0.305
LIG_SUMO_SIM_par_1	280	287	PF11976	0.391
LIG_TYR_ITIM	102	107	PF00017	0.478
LIG_TYR_ITIM	237	242	PF00017	0.269
LIG_UBA3_1	135	142	PF00899	0.255
LIG_UBA3_1	186	193	PF00899	0.254
MOD_CK1_1	128	134	PF00069	0.395
MOD_CK1_1	155	161	PF00069	0.390
MOD_CK1_1	203	209	PF00069	0.346
MOD_CK1_1	227	233	PF00069	0.379
MOD_CK1_1	319	325	PF00069	0.472
MOD_CK1_1	36	42	PF00069	0.434
MOD_CK1_1	80	86	PF00069	0.424
MOD_CK2_1	227	233	PF00069	0.322
MOD_CK2_1	298	304	PF00069	0.581
MOD_CK2_1	322	328	PF00069	0.423
MOD_CK2_1	91	97	PF00069	0.438
MOD_Cter_Amidation	60	63	PF01082	0.479
MOD_GlcNHglycan	114	117	PF01048	0.570
MOD_GlcNHglycan	121	124	PF01048	0.588
MOD_GlcNHglycan	170	173	PF01048	0.501
MOD_GlcNHglycan	218	221	PF01048	0.600
MOD_GlcNHglycan	226	229	PF01048	0.614
MOD_GlcNHglycan	230	233	PF01048	0.615
MOD_GlcNHglycan	242	245	PF01048	0.560
MOD_GlcNHglycan	249	253	PF01048	0.546
MOD_GlcNHglycan	25	28	PF01048	0.774
MOD_GlcNHglycan	261	264	PF01048	0.494
MOD_GlcNHglycan	266	269	PF01048	0.542
MOD_GlcNHglycan	324	327	PF01048	0.669
MOD_GlcNHglycan	338	341	PF01048	0.637
MOD_GlcNHglycan	370	373	PF01048	0.307
MOD_GlcNHglycan	79	82	PF01048	0.580
MOD_GlcNHglycan	99	102	PF01048	0.625
MOD_GSK3_1	121	128	PF00069	0.364
MOD_GSK3_1	130	137	PF00069	0.425
MOD_GSK3_1	140	147	PF00069	0.425
MOD_GSK3_1	152	159	PF00069	0.444
MOD_GSK3_1	200	207	PF00069	0.379
MOD_GSK3_1	224	231	PF00069	0.366
MOD_GSK3_1	288	295	PF00069	0.429
MOD_GSK3_1	300	307	PF00069	0.546
MOD_GSK3_1	311	318	PF00069	0.541
MOD_GSK3_1	32	39	PF00069	0.559
MOD_GSK3_1	350	357	PF00069	0.479
MOD_GSK3_1	64	71	PF00069	0.352
MOD_GSK3_1	73	80	PF00069	0.389
MOD_N-GLC_1	112	117	PF02516	0.599
MOD_N-GLC_2	297	299	PF02516	0.718
MOD_NEK2_1	112	117	PF00069	0.360
MOD_NEK2_1	121	126	PF00069	0.375
MOD_NEK2_1	165	170	PF00069	0.444
MOD_NEK2_1	189	194	PF00069	0.359
MOD_NEK2_1	21	26	PF00069	0.660
MOD_NEK2_1	213	218	PF00069	0.402
MOD_NEK2_1	298	303	PF00069	0.480
MOD_OFUCOSY	70	77	PF10250	0.588
MOD_PKA_1	62	68	PF00069	0.312
MOD_PKA_2	165	171	PF00069	0.276
MOD_PKA_2	213	219	PF00069	0.373
MOD_Plk_1	112	118	PF00069	0.394
MOD_Plk_1	248	254	PF00069	0.399
MOD_Plk_1	354	360	PF00069	0.351
MOD_Plk_2-3	348	354	PF00069	0.302
MOD_Plk_4	131	137	PF00069	0.411
MOD_Plk_4	182	188	PF00069	0.379
MOD_Plk_4	270	276	PF00069	0.405
MOD_Plk_4	45	51	PF00069	0.337
MOD_Plk_4	80	86	PF00069	0.376
MOD_ProDKin_1	311	317	PF00069	0.594
MOD_ProDKin_1	319	325	PF00069	0.583
MOD_SUMO_rev_2	285	291	PF00179	0.325
MOD_SUMO_rev_2	348	357	PF00179	0.319
MOD_SUMO_rev_2	97	107	PF00179	0.282
TRG_ENDOCYTIC_2	104	107	PF00928	0.484
TRG_ENDOCYTIC_2	239	242	PF00928	0.389
TRG_ER_diArg_1	5	7	PF00400	0.710
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.533

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P227	Leptomonas seymouri	31%	100%
A0A0N0P2L4	Leptomonas seymouri	42%	100%
A0A0N1HZ17	Leptomonas seymouri	30%	100%
A0A0N1I121	Leptomonas seymouri	28%	78%
A0A0N1I661	Leptomonas seymouri	36%	68%
A0A0S4IR61	Bodo saltans	27%	97%
A0A0S4JBV9	Bodo saltans	29%	80%
A0A0S4JEP2	Bodo saltans	22%	67%
A0A0S4JS89	Bodo saltans	25%	88%
A0A0S4JU20	Bodo saltans	36%	100%
A0A0S4KHE4	Bodo saltans	30%	100%
A0A0S4KK37	Bodo saltans	28%	67%
A0A3Q8I9A6	Leishmania donovani	67%	100%
A0A3Q8I9D0	Leishmania donovani	59%	100%
A0A3Q8IFC2	Leishmania donovani	42%	90%
A0A3Q8IIJ9	Leishmania donovani	36%	100%
A0A3Q8IK15	Leishmania donovani	39%	74%
A0A3S5H6D6	Leishmania donovani	31%	100%
A0A3S5H6L9	Leishmania donovani	61%	68%
A0A3S7WP69	Leishmania donovani	25%	100%
A0A3S7WPB2	Leishmania donovani	24%	100%
A0A3S7X4J4	Leishmania donovani	36%	100%
A4H4D2	Leishmania braziliensis	26%	100%
A4H4G6	Leishmania braziliensis	22%	100%
A4H5P0	Leishmania braziliensis	25%	100%
A4HBX3	Leishmania braziliensis	31%	86%
A4HJC8	Leishmania braziliensis	32%	100%
A4HSL2	Leishmania infantum	25%	100%
A4HTX9	Leishmania infantum	31%	100%
A4HVB0	Leishmania infantum	56%	97%
A4I6S2	Leishmania infantum	38%	89%
A4I6S3	Leishmania infantum	43%	78%
A4I6S4	Leishmania infantum	36%	100%
D1GJ51	Leishmania infantum	57%	84%
E8NHG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	88%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9ACQ0	Leishmania major	24%	100%
E9AEF4	Leishmania major	43%	85%
E9AG65	Leishmania infantum	24%	100%
E9AGG5	Leishmania infantum	63%	94%
E9AGG9	Leishmania infantum	46%	72%
E9AGH0	Leishmania infantum	69%	100%
E9AKJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AKM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9AMQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AP02	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	93%
E9AP05	Leishmania mexicana (strain MHOM/GT/2001/U1103)	56%	76%
E9AP07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9AP08	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	93%
E9AVA1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	85%
E9B1U3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	86%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	93%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
Q25331	Leishmania major	52%	89%
Q4PSE6	Arabidopsis thaliana	24%	90%
Q4Q6B6	Leishmania major	35%	100%
Q4Q6B7	Leishmania major	35%	100%
Q4Q6B8	Leishmania major	38%	74%
Q4QC79	Leishmania major	37%	86%
Q4QGI0	Leishmania major	51%	77%
Q4QGI2	Leishmania major	48%	70%
Q4QGI4	Leishmania major	49%	73%
Q4QGI6	Leishmania major	45%	71%
Q4QGJ4	Leishmania major	52%	89%
Q4QGJ6	Leishmania major	54%	68%
Q4QGJ7	Leishmania major	55%	89%
Q4QGJ9	Leishmania major	46%	67%
Q4QGK0	Leishmania major	52%	69%
Q4QGK2	Leishmania major	50%	70%
Q4QGK6	Leishmania major	52%	89%
Q4QGK8	Leishmania major	50%	76%
Q4QGL2	Leishmania major	50%	76%
Q4QGL4	Leishmania major	61%	95%
Q4QGL5	Leishmania major	51%	71%
Q4QHW6	Leishmania major	28%	100%
Q4QJB2	Leishmania major	26%	100%
Q9LHF1	Arabidopsis thaliana	23%	79%