Putative surface antigen protein 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

surface antigen protein 2, putative

Species:

Leishmania mexicana

UniProt:

E9AP02_LEIMU

TriTrypDb:

LmxM.12.0890

Length:

339

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 130

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 54, no: 11
NetGPI	no	yes: 0, no: 65

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	66
GO:0042995	cell projection	2	66
GO:0043226	organelle	2	66
GO:0043227	membrane-bounded organelle	3	66
GO:0110165	cellular anatomical entity	1	66
GO:0120025	plasma membrane bounded cell projection	3	66
GO:0016020	membrane	2	13
GO:0005886	plasma membrane	3	2

Expansion

Sequence features

E9AP02

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP02

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0004672	protein kinase activity	3	2
GO:0004674	protein serine/threonine kinase activity	4	2
GO:0016301	kinase activity	4	2
GO:0016740	transferase activity	2	2
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	2
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	2
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	6	8	PF00675	0.749
CLV_PCSK_KEX2_1	6	8	PF00082	0.749
CLV_PCSK_SKI1_1	7	11	PF00082	0.722
DEG_SPOP_SBC_1	284	288	PF00917	0.590
DEG_SPOP_SBC_1	292	296	PF00917	0.562
DOC_CYCLIN_yCln2_LP_2	10	16	PF00134	0.469
DOC_MAPK_gen_1	252	260	PF00069	0.572
DOC_MAPK_HePTP_8	102	114	PF00069	0.290
DOC_MAPK_MEF2A_6	105	114	PF00069	0.452
DOC_MAPK_MEF2A_6	182	191	PF00069	0.298
DOC_MAPK_MEF2A_6	252	260	PF00069	0.590
DOC_PP2B_LxvP_1	10	13	PF13499	0.494
DOC_USP7_MATH_1	291	295	PF00917	0.792
DOC_USP7_MATH_1	33	37	PF00917	0.640
DOC_WW_Pin1_4	287	292	PF00397	0.633
DOC_WW_Pin1_4	293	298	PF00397	0.597
LIG_14-3-3_CanoR_1	190	198	PF00244	0.584
LIG_14-3-3_CanoR_1	233	238	PF00244	0.477
LIG_Actin_WH2_2	153	168	PF00022	0.488
LIG_Actin_WH2_2	177	192	PF00022	0.315
LIG_BIR_II_1	1	5	PF00653	0.747
LIG_BRCT_BRCA1_1	70	74	PF00533	0.355
LIG_FHA_1	145	151	PF00498	0.553
LIG_FHA_1	44	50	PF00498	0.564
LIG_FHA_1	90	96	PF00498	0.480
LIG_FHA_2	204	210	PF00498	0.345
LIG_FHA_2	257	263	PF00498	0.563
LIG_FHA_2	275	281	PF00498	0.679
LIG_FHA_2	63	69	PF00498	0.557
LIG_FHA_2	92	98	PF00498	0.583
LIG_LIR_Gen_1	128	136	PF02991	0.445
LIG_LIR_Gen_1	225	234	PF02991	0.529
LIG_LIR_Gen_1	71	78	PF02991	0.362
LIG_LIR_Nem_3	103	107	PF02991	0.548
LIG_LIR_Nem_3	128	132	PF02991	0.386
LIG_LIR_Nem_3	214	218	PF02991	0.546
LIG_LIR_Nem_3	71	75	PF02991	0.409
LIG_PTAP_UEV_1	281	286	PF05743	0.550
LIG_SH2_CRK	215	219	PF00017	0.321
LIG_SH2_STAT5	215	218	PF00017	0.507
LIG_SH2_STAT5	91	94	PF00017	0.348
LIG_SH3_3	248	254	PF00018	0.495
LIG_SH3_3	279	285	PF00018	0.564
LIG_SH3_3	93	99	PF00018	0.595
LIG_SUMO_SIM_anti_2	106	111	PF11976	0.282
LIG_SUMO_SIM_anti_2	46	51	PF11976	0.356
LIG_SUMO_SIM_par_1	233	238	PF11976	0.318
LIG_SUMO_SIM_par_1	256	263	PF11976	0.566
LIG_TYR_ITIM	102	107	PF00017	0.602
LIG_TYR_ITIM	213	218	PF00017	0.311
LIG_UBA3_1	135	142	PF00899	0.298
MOD_CK1_1	128	134	PF00069	0.433
MOD_CK1_1	155	161	PF00069	0.468
MOD_CK1_1	203	209	PF00069	0.565
MOD_CK1_1	295	301	PF00069	0.677
MOD_CK1_1	36	42	PF00069	0.627
MOD_CK1_1	80	86	PF00069	0.468
MOD_CK2_1	203	209	PF00069	0.331
MOD_CK2_1	274	280	PF00069	0.667
MOD_CK2_1	298	304	PF00069	0.764
MOD_CK2_1	91	97	PF00069	0.416
MOD_Cter_Amidation	60	63	PF01082	0.324
MOD_GlcNHglycan	114	117	PF01048	0.501
MOD_GlcNHglycan	121	124	PF01048	0.521
MOD_GlcNHglycan	170	173	PF01048	0.456
MOD_GlcNHglycan	194	197	PF01048	0.550
MOD_GlcNHglycan	202	205	PF01048	0.546
MOD_GlcNHglycan	206	209	PF01048	0.545
MOD_GlcNHglycan	218	221	PF01048	0.493
MOD_GlcNHglycan	225	229	PF01048	0.509
MOD_GlcNHglycan	237	240	PF01048	0.558
MOD_GlcNHglycan	242	245	PF01048	0.594
MOD_GlcNHglycan	25	28	PF01048	0.650
MOD_GlcNHglycan	300	303	PF01048	0.567
MOD_GlcNHglycan	314	317	PF01048	0.620
MOD_GlcNHglycan	79	82	PF01048	0.502
MOD_GlcNHglycan	99	102	PF01048	0.597
MOD_GSK3_1	121	128	PF00069	0.506
MOD_GSK3_1	130	137	PF00069	0.449
MOD_GSK3_1	140	147	PF00069	0.570
MOD_GSK3_1	152	159	PF00069	0.459
MOD_GSK3_1	200	207	PF00069	0.510
MOD_GSK3_1	264	271	PF00069	0.583
MOD_GSK3_1	276	283	PF00069	0.708
MOD_GSK3_1	287	294	PF00069	0.766
MOD_GSK3_1	32	39	PF00069	0.604
MOD_GSK3_1	326	333	PF00069	0.587
MOD_GSK3_1	64	71	PF00069	0.457
MOD_GSK3_1	73	80	PF00069	0.480
MOD_N-GLC_1	112	117	PF02516	0.560
MOD_N-GLC_2	273	275	PF02516	0.639
MOD_NEK2_1	112	117	PF00069	0.511
MOD_NEK2_1	121	126	PF00069	0.534
MOD_NEK2_1	165	170	PF00069	0.507
MOD_NEK2_1	189	194	PF00069	0.632
MOD_NEK2_1	21	26	PF00069	0.588
MOD_NEK2_1	274	279	PF00069	0.645
MOD_OFUCOSY	70	77	PF10250	0.480
MOD_PKA_1	62	68	PF00069	0.354
MOD_PKA_2	165	171	PF00069	0.390
MOD_PKA_2	189	195	PF00069	0.339
MOD_Plk_1	112	118	PF00069	0.579
MOD_Plk_1	224	230	PF00069	0.363
MOD_Plk_1	330	336	PF00069	0.410
MOD_Plk_2-3	324	330	PF00069	0.361
MOD_Plk_4	131	137	PF00069	0.376
MOD_Plk_4	246	252	PF00069	0.503
MOD_Plk_4	45	51	PF00069	0.405
MOD_Plk_4	80	86	PF00069	0.460
MOD_ProDKin_1	287	293	PF00069	0.621
MOD_ProDKin_1	295	301	PF00069	0.549
MOD_SUMO_rev_2	261	267	PF00179	0.408
MOD_SUMO_rev_2	324	333	PF00179	0.383
MOD_SUMO_rev_2	97	107	PF00179	0.336
TRG_ENDOCYTIC_2	104	107	PF00928	0.608
TRG_ENDOCYTIC_2	215	218	PF00928	0.512
TRG_ER_diArg_1	5	7	PF00400	0.735
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.373

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P276	Leptomonas seymouri	33%	100%
A0A0N0P2L4	Leptomonas seymouri	45%	100%
A0A0N0P4M2	Leptomonas seymouri	26%	100%
A0A0N1HZ17	Leptomonas seymouri	30%	100%
A0A0S4IR61	Bodo saltans	24%	84%
A0A0S4IVN7	Bodo saltans	36%	89%
A0A0S4J805	Bodo saltans	34%	100%
A0A0S4JBV9	Bodo saltans	27%	69%
A0A0S4JU20	Bodo saltans	33%	100%
A0A3Q8I9A6	Leishmania donovani	63%	88%
A0A3Q8I9D0	Leishmania donovani	56%	100%
A0A3Q8IC27	Leishmania donovani	40%	71%
A0A3Q8IFC2	Leishmania donovani	46%	78%
A0A3Q8IIJ9	Leishmania donovani	35%	100%
A0A3S5H6D6	Leishmania donovani	33%	100%
A0A3S7WP69	Leishmania donovani	20%	100%
A0A3S7WPB2	Leishmania donovani	25%	100%
A0A3S7X4J4	Leishmania donovani	35%	100%
A4H4D2	Leishmania braziliensis	23%	100%
A4H4G6	Leishmania braziliensis	25%	100%
A4H5P0	Leishmania braziliensis	28%	100%
A4HBX3	Leishmania braziliensis	37%	75%
A4HJC8	Leishmania braziliensis	30%	100%
A4HJX1	Leishmania braziliensis	37%	77%
A4HSL2	Leishmania infantum	20%	100%
A4HTX9	Leishmania infantum	32%	100%
A4HVB0	Leishmania infantum	52%	84%
A4HZ93	Leishmania infantum	40%	71%
A4I6S2	Leishmania infantum	42%	77%
A4I6S3	Leishmania infantum	46%	68%
A4I6S4	Leishmania infantum	35%	100%
D1GJ51	Leishmania infantum	49%	73%
E8NHG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	76%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9ACQ0	Leishmania major	26%	100%
E9AEF4	Leishmania major	46%	74%
E9AG65	Leishmania infantum	25%	100%
E9AGG5	Leishmania infantum	58%	81%
E9AGH0	Leishmania infantum	63%	90%
E9AKJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	20%	100%
E9AKM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AMQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AP01	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
E9AP03	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	87%
E9AP07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	92%
E9AP08	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	81%
E9AVA1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	74%
E9B1U3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	75%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	99%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	81%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
P21978	Leishmania amazonensis	62%	71%
Q25331	Leishmania major	55%	100%
Q4Q6B6	Leishmania major	32%	100%
Q4Q6B7	Leishmania major	32%	100%
Q4QC79	Leishmania major	42%	74%
Q4QGI0	Leishmania major	53%	67%
Q4QGJ4	Leishmania major	54%	100%
Q4QGJ7	Leishmania major	57%	100%
Q4QGK6	Leishmania major	55%	100%
Q4QGK8	Leishmania major	50%	66%
Q4QGL2	Leishmania major	50%	66%
Q4QGL4	Leishmania major	55%	100%
Q4QHW6	Leishmania major	30%	100%
Q4QJB2	Leishmania major	21%	100%