TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania mexicana

UniProt:

E9ANS4_LEIMU

TriTrypDb:

LmxM.11.1370

Length:

618

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ANS4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ANS4

PDB structure(s): 7am2_CA

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	439	443	PF00656	0.440
CLV_C14_Caspase3-7	87	91	PF00656	0.347
CLV_NRD_NRD_1	106	108	PF00675	0.405
CLV_NRD_NRD_1	143	145	PF00675	0.482
CLV_NRD_NRD_1	299	301	PF00675	0.397
CLV_NRD_NRD_1	359	361	PF00675	0.524
CLV_NRD_NRD_1	424	426	PF00675	0.363
CLV_NRD_NRD_1	44	46	PF00675	0.476
CLV_NRD_NRD_1	508	510	PF00675	0.457
CLV_NRD_NRD_1	553	555	PF00675	0.367
CLV_NRD_NRD_1	574	576	PF00675	0.511
CLV_NRD_NRD_1	577	579	PF00675	0.521
CLV_PCSK_FUR_1	575	579	PF00082	0.407
CLV_PCSK_KEX2_1	143	145	PF00082	0.527
CLV_PCSK_KEX2_1	299	301	PF00082	0.397
CLV_PCSK_KEX2_1	424	426	PF00082	0.365
CLV_PCSK_KEX2_1	44	46	PF00082	0.476
CLV_PCSK_KEX2_1	508	510	PF00082	0.457
CLV_PCSK_KEX2_1	553	555	PF00082	0.385
CLV_PCSK_KEX2_1	576	578	PF00082	0.434
CLV_PCSK_PC1ET2_1	576	578	PF00082	0.403
CLV_PCSK_PC7_1	504	510	PF00082	0.393
CLV_PCSK_SKI1_1	113	117	PF00082	0.399
CLV_PCSK_SKI1_1	249	253	PF00082	0.278
CLV_PCSK_SKI1_1	356	360	PF00082	0.502
CLV_PCSK_SKI1_1	416	420	PF00082	0.411
CLV_PCSK_SKI1_1	601	605	PF00082	0.335
CLV_Separin_Metazoa	235	239	PF03568	0.500
CLV_Separin_Metazoa	357	361	PF03568	0.387
DEG_APCC_DBOX_1	248	256	PF00400	0.284
DEG_APCC_DBOX_1	355	363	PF00400	0.387
DEG_Nend_UBRbox_1	1	4	PF02207	0.408
DEG_SPOP_SBC_1	375	379	PF00917	0.374
DOC_CYCLIN_RxL_1	246	256	PF00134	0.356
DOC_MAPK_gen_1	107	117	PF00069	0.405
DOC_MAPK_gen_1	170	179	PF00069	0.398
DOC_MAPK_gen_1	482	488	PF00069	0.503
DOC_PP1_RVXF_1	407	414	PF00149	0.399
DOC_PP2B_LxvP_1	453	456	PF13499	0.377
DOC_PP2B_PxIxI_1	236	242	PF00149	0.248
DOC_PP4_FxxP_1	124	127	PF00568	0.394
DOC_PP4_FxxP_1	588	591	PF00568	0.435
DOC_USP7_MATH_1	127	131	PF00917	0.432
DOC_USP7_MATH_1	18	22	PF00917	0.520
DOC_USP7_MATH_1	190	194	PF00917	0.356
DOC_USP7_MATH_1	212	216	PF00917	0.377
DOC_USP7_MATH_1	295	299	PF00917	0.518
DOC_USP7_MATH_1	302	306	PF00917	0.508
DOC_USP7_MATH_1	330	334	PF00917	0.375
DOC_USP7_MATH_1	404	408	PF00917	0.298
DOC_USP7_MATH_1	441	445	PF00917	0.542
DOC_USP7_MATH_1	468	472	PF00917	0.501
DOC_USP7_MATH_1	530	534	PF00917	0.422
DOC_USP7_MATH_1	591	595	PF00917	0.381
DOC_USP7_MATH_2	543	549	PF00917	0.487
DOC_WW_Pin1_4	119	124	PF00397	0.447
DOC_WW_Pin1_4	129	134	PF00397	0.345
DOC_WW_Pin1_4	314	319	PF00397	0.465
DOC_WW_Pin1_4	494	499	PF00397	0.607
DOC_WW_Pin1_4	592	597	PF00397	0.341
DOC_WW_Pin1_4	62	67	PF00397	0.686
LIG_14-3-3_CanoR_1	111	116	PF00244	0.430
LIG_14-3-3_CanoR_1	134	139	PF00244	0.403
LIG_14-3-3_CanoR_1	238	242	PF00244	0.543
LIG_14-3-3_CanoR_1	267	275	PF00244	0.377
LIG_14-3-3_CanoR_1	409	414	PF00244	0.506
LIG_14-3-3_CanoR_1	51	56	PF00244	0.524
LIG_14-3-3_CanoR_1	78	86	PF00244	0.476
LIG_Actin_WH2_2	141	158	PF00022	0.354
LIG_APCC_ABBA_1	157	162	PF00400	0.284
LIG_APCC_ABBAyCdc20_2	156	162	PF00400	0.302
LIG_BIR_III_4	90	94	PF00653	0.423
LIG_BRCT_BRCA1_1	270	274	PF00533	0.447
LIG_BRCT_BRCA1_1	484	488	PF00533	0.328
LIG_BRCT_BRCA1_1	532	536	PF00533	0.419
LIG_BRCT_BRCA1_1	563	567	PF00533	0.371
LIG_deltaCOP1_diTrp_1	150	157	PF00928	0.223
LIG_EH1_1	217	225	PF00400	0.351
LIG_eIF4E_1	218	224	PF01652	0.351
LIG_eIF4E_1	246	252	PF01652	0.343
LIG_FHA_1	112	118	PF00498	0.391
LIG_FHA_1	353	359	PF00498	0.421
LIG_FHA_1	467	473	PF00498	0.414
LIG_FHA_2	287	293	PF00498	0.450
LIG_FHA_2	310	316	PF00498	0.448
LIG_FHA_2	376	382	PF00498	0.353
LIG_FHA_2	445	451	PF00498	0.459
LIG_FHA_2	608	614	PF00498	0.442
LIG_FHA_2	85	91	PF00498	0.341
LIG_HP1_1	596	600	PF01393	0.354
LIG_Integrin_RGD_1	30	32	PF01839	0.378
LIG_Integrin_RGDW_4	30	33	PF00362	0.363
LIG_LIR_Apic_2	122	127	PF02991	0.391
LIG_LIR_Gen_1	193	202	PF02991	0.446
LIG_LIR_Gen_1	227	237	PF02991	0.447
LIG_LIR_Gen_1	341	349	PF02991	0.399
LIG_LIR_Gen_1	474	481	PF02991	0.405
LIG_LIR_Gen_1	50	60	PF02991	0.329
LIG_LIR_Gen_1	594	604	PF02991	0.321
LIG_LIR_Gen_1	610	617	PF02991	0.278
LIG_LIR_Nem_3	137	141	PF02991	0.369
LIG_LIR_Nem_3	150	155	PF02991	0.329
LIG_LIR_Nem_3	193	197	PF02991	0.301
LIG_LIR_Nem_3	227	233	PF02991	0.433
LIG_LIR_Nem_3	271	277	PF02991	0.301
LIG_LIR_Nem_3	341	345	PF02991	0.554
LIG_LIR_Nem_3	474	480	PF02991	0.415
LIG_LIR_Nem_3	50	55	PF02991	0.270
LIG_LIR_Nem_3	506	510	PF02991	0.384
LIG_LIR_Nem_3	594	600	PF02991	0.366
LIG_LIR_Nem_3	610	614	PF02991	0.324
LIG_PCNA_yPIPBox_3	445	459	PF02747	0.330
LIG_Pex14_1	547	551	PF04695	0.515
LIG_Pex14_2	274	278	PF04695	0.284
LIG_Pex14_2	476	480	PF04695	0.496
LIG_SH2_CRK	507	511	PF00017	0.365
LIG_SH2_GRB2like	327	330	PF00017	0.347
LIG_SH2_NCK_1	327	331	PF00017	0.487
LIG_SH2_SRC	175	178	PF00017	0.476
LIG_SH2_SRC	327	330	PF00017	0.347
LIG_SH2_STAT5	141	144	PF00017	0.388
LIG_SH2_STAT5	185	188	PF00017	0.425
LIG_SH2_STAT5	246	249	PF00017	0.487
LIG_SH2_STAT5	277	280	PF00017	0.286
LIG_SH2_STAT5	309	312	PF00017	0.354
LIG_SH2_STAT5	324	327	PF00017	0.255
LIG_SH2_STAT5	446	449	PF00017	0.448
LIG_SH3_3	448	454	PF00018	0.323
LIG_SH3_3	495	501	PF00018	0.564
LIG_SH3_3	508	514	PF00018	0.351
LIG_SH3_3	552	558	PF00018	0.376
LIG_TRAF2_1	40	43	PF00917	0.362
LIG_TYR_ITIM	275	280	PF00017	0.286
LIG_WRC_WIRS_1	280	285	PF05994	0.320
LIG_WRC_WIRS_1	410	415	PF05994	0.418
LIG_WRC_WIRS_1	608	613	PF05994	0.392
MOD_CDK_SPK_2	129	134	PF00069	0.361
MOD_CDK_SPK_2	494	499	PF00069	0.543
MOD_CK1_1	165	171	PF00069	0.361
MOD_CK1_1	352	358	PF00069	0.239
MOD_CK1_1	412	418	PF00069	0.476
MOD_CK1_1	444	450	PF00069	0.428
MOD_CK2_1	122	128	PF00069	0.456
MOD_CK2_1	134	140	PF00069	0.372
MOD_CK2_1	253	259	PF00069	0.319
MOD_CK2_1	295	301	PF00069	0.509
MOD_CK2_1	309	315	PF00069	0.363
MOD_CK2_1	37	43	PF00069	0.389
MOD_CK2_1	375	381	PF00069	0.535
MOD_CK2_1	458	464	PF00069	0.462
MOD_CK2_1	468	474	PF00069	0.419
MOD_Cter_Amidation	551	554	PF01082	0.343
MOD_GlcNHglycan	20	23	PF01048	0.517
MOD_GlcNHglycan	255	258	PF01048	0.415
MOD_GlcNHglycan	38	42	PF01048	0.323
MOD_GlcNHglycan	394	398	PF01048	0.337
MOD_GlcNHglycan	425	428	PF01048	0.461
MOD_GlcNHglycan	460	463	PF01048	0.405
MOD_GlcNHglycan	55	58	PF01048	0.374
MOD_GSK3_1	107	114	PF00069	0.404
MOD_GSK3_1	370	377	PF00069	0.393
MOD_GSK3_1	47	54	PF00069	0.452
MOD_GSK3_1	541	548	PF00069	0.476
MOD_N-GLC_1	561	566	PF02516	0.381
MOD_NEK2_1	12	17	PF00069	0.564
MOD_NEK2_2	286	291	PF00069	0.444
MOD_PIKK_1	195	201	PF00454	0.299
MOD_PIKK_1	365	371	PF00454	0.544
MOD_PIKK_1	404	410	PF00454	0.300
MOD_PIKK_1	441	447	PF00454	0.586
MOD_PIKK_1	475	481	PF00454	0.295
MOD_PKA_1	107	113	PF00069	0.404
MOD_PKA_1	482	488	PF00069	0.360
MOD_PKA_2	237	243	PF00069	0.502
MOD_PKA_2	423	429	PF00069	0.508
MOD_PKA_2	458	464	PF00069	0.662
MOD_PKA_2	503	509	PF00069	0.378
MOD_PKA_2	569	575	PF00069	0.508
MOD_PKB_1	105	113	PF00069	0.534
MOD_PKB_1	265	273	PF00069	0.393
MOD_PKB_1	554	562	PF00069	0.484
MOD_Plk_1	127	133	PF00069	0.500
MOD_Plk_1	226	232	PF00069	0.415
MOD_Plk_1	330	336	PF00069	0.462
MOD_Plk_1	441	447	PF00069	0.505
MOD_Plk_1	580	586	PF00069	0.489
MOD_Plk_2-3	162	168	PF00069	0.459
MOD_Plk_2-3	436	442	PF00069	0.542
MOD_Plk_2-3	545	551	PF00069	0.514
MOD_Plk_4	111	117	PF00069	0.395
MOD_Plk_4	286	292	PF00069	0.433
MOD_Plk_4	484	490	PF00069	0.368
MOD_ProDKin_1	119	125	PF00069	0.448
MOD_ProDKin_1	129	135	PF00069	0.344
MOD_ProDKin_1	314	320	PF00069	0.462
MOD_ProDKin_1	494	500	PF00069	0.606
MOD_ProDKin_1	592	598	PF00069	0.339
MOD_ProDKin_1	62	68	PF00069	0.678
MOD_SUMO_rev_2	368	378	PF00179	0.317
TRG_DiLeu_BaEn_1	354	359	PF01217	0.323
TRG_DiLeu_BaLyEn_6	406	411	PF01217	0.314
TRG_DiLeu_LyEn_5	150	155	PF01217	0.425
TRG_ENDOCYTIC_2	230	233	PF00928	0.435
TRG_ENDOCYTIC_2	277	280	PF00928	0.281
TRG_ENDOCYTIC_2	507	510	PF00928	0.361
TRG_ENDOCYTIC_2	608	611	PF00928	0.328
TRG_ER_diArg_1	142	144	PF00400	0.518
TRG_ER_diArg_1	169	172	PF00400	0.420
TRG_ER_diArg_1	423	425	PF00400	0.443
TRG_ER_diArg_1	507	509	PF00400	0.470
TRG_ER_diArg_1	553	556	PF00400	0.434
TRG_ER_diArg_1	574	577	PF00400	0.559
TRG_NLS_MonoCore_2	574	579	PF00514	0.396
TRG_NLS_MonoExtC_3	575	581	PF00514	0.394
TRG_NLS_MonoExtN_4	575	580	PF00514	0.396

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P414	Leptomonas seymouri	63%	98%
A0A0S4JV05	Bodo saltans	40%	94%
A0A1X0NUT8	Trypanosomatidae	43%	100%
A0A3S7WRT6	Leishmania donovani	91%	100%
A0A422N563	Trypanosoma rangeli	45%	100%
A4H6R0	Leishmania braziliensis	81%	100%
A4HV43	Leishmania infantum	91%	100%
D0A7A5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	99%
Q4QGU5	Leishmania major	91%	100%
V5BBF7	Trypanosoma cruzi	44%	100%