Putative eukaryotic release factor 3

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic release factor 3, putative

Species:

Leishmania mexicana

UniProt:

E9ANQ6_LEIMU

TriTrypDb:

LmxM.11.1170

Length:

767

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0018444	translation release factor complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ANQ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ANQ6

Function

Biological processes
Term	Name	Level	Count
GO:0006412	translation	4	1
GO:0006518	peptide metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0019538	protein metabolic process	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	1
GO:0043043	peptide biosynthetic process	5	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043603	amide metabolic process	3	1
GO:0043604	amide biosynthetic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0003924	GTPase activity	7	11
GO:0005488	binding	1	11
GO:0005525	GTP binding	5	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0019001	guanyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032561	guanyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003676	nucleic acid binding	3	2
GO:0003747	translation release factor activity	5	1
GO:0008079	translation termination factor activity	4	1
GO:0008135	translation factor activity, RNA binding	3	2
GO:0045182	translation regulator activity	1	2
GO:0090079	translation regulator activity, nucleic acid binding	2	2
GO:0003746	translation elongation factor activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	183	187	PF00656	0.748
CLV_C14_Caspase3-7	241	245	PF00656	0.497
CLV_C14_Caspase3-7	411	415	PF00656	0.447
CLV_NRD_NRD_1	304	306	PF00675	0.586
CLV_NRD_NRD_1	366	368	PF00675	0.261
CLV_NRD_NRD_1	408	410	PF00675	0.247
CLV_NRD_NRD_1	693	695	PF00675	0.299
CLV_PCSK_KEX2_1	304	306	PF00082	0.626
CLV_PCSK_KEX2_1	324	326	PF00082	0.629
CLV_PCSK_KEX2_1	366	368	PF00082	0.236
CLV_PCSK_KEX2_1	407	409	PF00082	0.248
CLV_PCSK_PC1ET2_1	324	326	PF00082	0.631
CLV_PCSK_PC1ET2_1	407	409	PF00082	0.258
CLV_PCSK_SKI1_1	392	396	PF00082	0.258
CLV_PCSK_SKI1_1	408	412	PF00082	0.211
CLV_PCSK_SKI1_1	496	500	PF00082	0.247
CLV_PCSK_SKI1_1	671	675	PF00082	0.261
CLV_PCSK_SKI1_1	736	740	PF00082	0.244
DEG_SPOP_SBC_1	273	277	PF00917	0.693
DOC_ANK_TNKS_1	743	750	PF00023	0.551
DOC_CKS1_1	145	150	PF01111	0.596
DOC_CKS1_1	249	254	PF01111	0.682
DOC_CYCLIN_yCln2_LP_2	719	725	PF00134	0.527
DOC_MAPK_gen_1	204	213	PF00069	0.635
DOC_MAPK_gen_1	324	335	PF00069	0.312
DOC_MAPK_gen_1	508	517	PF00069	0.464
DOC_MAPK_gen_1	538	545	PF00069	0.515
DOC_MAPK_MEF2A_6	744	752	PF00069	0.482
DOC_PP1_RVXF_1	508	515	PF00149	0.461
DOC_PP1_RVXF_1	646	653	PF00149	0.373
DOC_PP2B_LxvP_1	413	416	PF13499	0.447
DOC_PP2B_LxvP_1	549	552	PF13499	0.439
DOC_PP4_FxxP_1	561	564	PF00568	0.444
DOC_USP7_MATH_1	173	177	PF00917	0.714
DOC_USP7_MATH_1	180	184	PF00917	0.652
DOC_USP7_MATH_1	233	237	PF00917	0.781
DOC_USP7_MATH_1	247	251	PF00917	0.760
DOC_USP7_MATH_1	341	345	PF00917	0.447
DOC_USP7_MATH_2	226	232	PF00917	0.697
DOC_USP7_UBL2_3	162	166	PF12436	0.503
DOC_USP7_UBL2_3	294	298	PF12436	0.654
DOC_USP7_UBL2_3	320	324	PF12436	0.637
DOC_USP7_UBL2_3	570	574	PF12436	0.489
DOC_USP7_UBL2_3	687	691	PF12436	0.438
DOC_USP7_UBL2_3	729	733	PF12436	0.447
DOC_WW_Pin1_4	144	149	PF00397	0.712
DOC_WW_Pin1_4	15	20	PF00397	0.702
DOC_WW_Pin1_4	231	236	PF00397	0.730
DOC_WW_Pin1_4	248	253	PF00397	0.745
DOC_WW_Pin1_4	258	263	PF00397	0.770
DOC_WW_Pin1_4	297	302	PF00397	0.604
LIG_14-3-3_CanoR_1	408	413	PF00244	0.477
LIG_APCC_ABBA_1	725	730	PF00400	0.499
LIG_APCC_ABBAyCdc20_2	80	86	PF00400	0.689
LIG_BIR_II_1	1	5	PF00653	0.608
LIG_BIR_III_4	414	418	PF00653	0.447
LIG_BRCT_BRCA1_1	609	613	PF00533	0.508
LIG_BRCT_BRCA1_2	609	615	PF00533	0.501
LIG_FHA_1	208	214	PF00498	0.653
LIG_FHA_1	463	469	PF00498	0.447
LIG_FHA_1	5	11	PF00498	0.688
LIG_FHA_1	694	700	PF00498	0.447
LIG_FHA_1	743	749	PF00498	0.447
LIG_FHA_2	222	228	PF00498	0.734
LIG_FHA_2	235	241	PF00498	0.798
LIG_FHA_2	269	275	PF00498	0.779
LIG_FHA_2	290	296	PF00498	0.637
LIG_FHA_2	298	304	PF00498	0.531
LIG_FHA_2	409	415	PF00498	0.447
LIG_FHA_2	428	434	PF00498	0.314
LIG_FHA_2	446	452	PF00498	0.447
LIG_FHA_2	539	545	PF00498	0.472
LIG_FHA_2	554	560	PF00498	0.426
LIG_FHA_2	595	601	PF00498	0.433
LIG_FHA_2	609	615	PF00498	0.355
LIG_FXI_DFP_1	728	732	PF00024	0.261
LIG_IRF3_LxIS_1	434	440	PF10401	0.447
LIG_LIR_Apic_2	2	6	PF02991	0.619
LIG_LIR_Apic_2	47	51	PF02991	0.455
LIG_LIR_Apic_2	558	564	PF02991	0.466
LIG_LIR_Gen_1	378	385	PF02991	0.447
LIG_LIR_Gen_1	486	495	PF02991	0.465
LIG_LIR_Gen_1	682	692	PF02991	0.456
LIG_LIR_Gen_1	730	739	PF02991	0.447
LIG_LIR_Nem_3	378	382	PF02991	0.447
LIG_LIR_Nem_3	486	491	PF02991	0.465
LIG_LIR_Nem_3	575	581	PF02991	0.477
LIG_LIR_Nem_3	682	688	PF02991	0.456
LIG_LIR_Nem_3	730	734	PF02991	0.481
LIG_PCNA_APIM_2	734	740	PF02747	0.447
LIG_Pex14_1	377	381	PF04695	0.447
LIG_Pex14_2	331	335	PF04695	0.461
LIG_Pex14_2	446	450	PF04695	0.447
LIG_REV1ctd_RIR_1	650	660	PF16727	0.499
LIG_SH2_CRK	100	104	PF00017	0.783
LIG_SH2_CRK	379	383	PF00017	0.447
LIG_SH2_GRB2like	24	27	PF00017	0.668
LIG_SH2_GRB2like	63	66	PF00017	0.664
LIG_SH2_SRC	379	382	PF00017	0.447
LIG_SH2_SRC	84	87	PF00017	0.721
LIG_SH2_STAP1	379	383	PF00017	0.447
LIG_SH2_STAT3	121	124	PF00017	0.709
LIG_SH2_STAT3	138	141	PF00017	0.434
LIG_SH2_STAT3	28	31	PF00017	0.667
LIG_SH2_STAT3	49	52	PF00017	0.660
LIG_SH2_STAT3	74	77	PF00017	0.680
LIG_SH2_STAT5	17	20	PF00017	0.709
LIG_SH2_STAT5	381	384	PF00017	0.447
LIG_SH2_STAT5	402	405	PF00017	0.447
LIG_SH2_STAT5	638	641	PF00017	0.358
LIG_SH3_3	197	203	PF00018	0.628
LIG_SH3_3	246	252	PF00018	0.737
LIG_SH3_3	721	727	PF00018	0.499
LIG_SH3_3	9	15	PF00018	0.610
LIG_SUMO_SIM_par_1	435	441	PF11976	0.447
LIG_SUMO_SIM_par_1	656	661	PF11976	0.447
LIG_SUMO_SIM_par_1	720	726	PF11976	0.527
LIG_SxIP_EBH_1	636	648	PF03271	0.417
LIG_TRAF2_1	225	228	PF00917	0.736
LIG_TRAF2_1	262	265	PF00917	0.683
LIG_TRAF2_1	300	303	PF00917	0.557
LIG_TRAF2_1	386	389	PF00917	0.478
LIG_TRAF2_1	678	681	PF00917	0.447
LIG_UBA3_1	471	477	PF00899	0.461
LIG_UBA3_1	542	550	PF00899	0.551
LIG_UBA3_1	688	695	PF00899	0.493
MOD_CDK_SPxxK_3	297	304	PF00069	0.695
MOD_CK1_1	231	237	PF00069	0.770
MOD_CK1_1	344	350	PF00069	0.447
MOD_CK1_1	440	446	PF00069	0.461
MOD_CK1_1	607	613	PF00069	0.489
MOD_CK1_1	642	648	PF00069	0.395
MOD_CK1_1	667	673	PF00069	0.501
MOD_CK2_1	221	227	PF00069	0.731
MOD_CK2_1	234	240	PF00069	0.661
MOD_CK2_1	268	274	PF00069	0.719
MOD_CK2_1	297	303	PF00069	0.559
MOD_CK2_1	383	389	PF00069	0.447
MOD_CK2_1	445	451	PF00069	0.446
MOD_CK2_1	538	544	PF00069	0.449
MOD_CK2_1	551	557	PF00069	0.375
MOD_CK2_1	594	600	PF00069	0.399
MOD_CK2_1	608	614	PF00069	0.391
MOD_CK2_1	675	681	PF00069	0.551
MOD_CK2_1	754	760	PF00069	0.452
MOD_Cter_Amidation	213	216	PF01082	0.673
MOD_GlcNHglycan	110	113	PF01048	0.766
MOD_GlcNHglycan	182	185	PF01048	0.726
MOD_GlcNHglycan	195	198	PF01048	0.687
MOD_GlcNHglycan	265	269	PF01048	0.736
MOD_GlcNHglycan	280	283	PF01048	0.662
MOD_GlcNHglycan	346	349	PF01048	0.247
MOD_GlcNHglycan	582	585	PF01048	0.360
MOD_GlcNHglycan	666	669	PF01048	0.327
MOD_GlcNHglycan	677	680	PF01048	0.351
MOD_GSK3_1	231	238	PF00069	0.691
MOD_GSK3_1	264	271	PF00069	0.781
MOD_GSK3_1	274	281	PF00069	0.683
MOD_GSK3_1	388	395	PF00069	0.478
MOD_GSK3_1	604	611	PF00069	0.400
MOD_GSK3_1	638	645	PF00069	0.388
MOD_GSK3_1	754	761	PF00069	0.459
MOD_N-GLC_1	64	69	PF02516	0.659
MOD_NEK2_1	221	226	PF00069	0.643
MOD_NEK2_1	437	442	PF00069	0.447
MOD_NEK2_1	462	467	PF00069	0.447
MOD_NEK2_1	73	78	PF00069	0.470
MOD_NEK2_1	754	759	PF00069	0.439
MOD_PIKK_1	228	234	PF00454	0.704
MOD_PIKK_1	268	274	PF00454	0.788
MOD_PIKK_1	427	433	PF00454	0.447
MOD_PIKK_1	73	79	PF00454	0.735
MOD_PKA_1	408	414	PF00069	0.447
MOD_PKA_2	395	401	PF00069	0.451
MOD_PKA_2	408	414	PF00069	0.406
MOD_PKA_2	693	699	PF00069	0.499
MOD_PKB_1	314	322	PF00069	0.685
MOD_Plk_1	681	687	PF00069	0.461
MOD_Plk_2-3	553	559	PF00069	0.452
MOD_Plk_2-3	604	610	PF00069	0.396
MOD_Plk_4	408	414	PF00069	0.443
MOD_Plk_4	421	427	PF00069	0.415
MOD_Plk_4	538	544	PF00069	0.462
MOD_ProDKin_1	144	150	PF00069	0.711
MOD_ProDKin_1	15	21	PF00069	0.702
MOD_ProDKin_1	231	237	PF00069	0.730
MOD_ProDKin_1	248	254	PF00069	0.749
MOD_ProDKin_1	258	264	PF00069	0.769
MOD_ProDKin_1	297	303	PF00069	0.599
MOD_SUMO_for_1	308	311	PF00179	0.567
MOD_SUMO_rev_2	234	243	PF00179	0.749
MOD_SUMO_rev_2	479	484	PF00179	0.525
MOD_SUMO_rev_2	544	552	PF00179	0.556
MOD_SUMO_rev_2	571	575	PF00179	0.488
TRG_DiLeu_BaEn_1	458	463	PF01217	0.447
TRG_ENDOCYTIC_2	379	382	PF00928	0.447
TRG_ENDOCYTIC_2	488	491	PF00928	0.447
TRG_ENDOCYTIC_2	578	581	PF00928	0.369
TRG_ER_diArg_1	365	367	PF00400	0.461
TRG_ER_diArg_1	408	410	PF00400	0.446
TRG_ER_diArg_1	494	497	PF00400	0.340
TRG_NLS_Bipartite_1	204	219	PF00514	0.750
TRG_NLS_MonoExtN_4	203	208	PF00514	0.615
TRG_Pf-PMV_PEXEL_1	627	631	PF00026	0.455

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAZ5	Leptomonas seymouri	78%	100%
A0A0S4IX91	Bodo saltans	62%	100%
A0A1X0NV54	Trypanosomatidae	71%	100%
A0A3Q8ICH2	Leishmania donovani	93%	100%
A0A3R7MLL0	Trypanosoma rangeli	71%	100%
A0A3S7X9T0	Leishmania donovani	32%	100%
A4H6P3	Leishmania braziliensis	88%	100%
A4HN87	Leishmania braziliensis	32%	100%
A4HV24	Leishmania infantum	92%	100%
A4IBV5	Leishmania infantum	31%	100%
C9ZYQ4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
D0A7C5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
E9AFP2	Leishmania major	31%	100%
E9B6U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
O13354	Candida albicans	41%	100%
O74718	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	100%
P23637	Ogataea pini	40%	100%
Q2KHZ2	Bos taurus	35%	100%
Q4QGW5	Leishmania major	97%	100%
Q9HGI7	Candida maltosa	39%	100%
Q9HGI8	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	38%	100%