Putative aminopeptidase (Metallo-peptidase, clan mf, family m17)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

metallo-peptidase, Clan MF, Family M17

Species:

Leishmania mexicana

UniProt:

E9ANK8_LEIMU

TriTrypDb:

LmxM.11.0630

Length:

535

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 8
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 12

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 20
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	21
GO:0110165	cellular anatomical entity	1	21
GO:0005654	nucleoplasm	2	1

Expansion

Sequence features

E9ANK8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ANK8

PDB structure(s): 5nth_A

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	21
GO:0006807	nitrogen compound metabolic process	2	21
GO:0008152	metabolic process	1	21
GO:0019538	protein metabolic process	3	21
GO:0043170	macromolecule metabolic process	3	21
GO:0044238	primary metabolic process	2	21
GO:0071704	organic substance metabolic process	2	21
GO:1901564	organonitrogen compound metabolic process	3	21

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	21
GO:0004177	aminopeptidase activity	5	21
GO:0005488	binding	1	21
GO:0008233	peptidase activity	3	21
GO:0008235	metalloexopeptidase activity	5	21
GO:0008237	metallopeptidase activity	4	21
GO:0008238	exopeptidase activity	4	21
GO:0016787	hydrolase activity	2	21
GO:0030145	manganese ion binding	6	21
GO:0043167	ion binding	2	21
GO:0043169	cation binding	3	21
GO:0046872	metal ion binding	4	21
GO:0046914	transition metal ion binding	5	21
GO:0070006	metalloaminopeptidase activity	6	21
GO:0140096	catalytic activity, acting on a protein	2	21

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_KEX2_1	2	4	PF00082	0.516
CLV_PCSK_PC1ET2_1	2	4	PF00082	0.516
CLV_PCSK_SKI1_1	165	169	PF00082	0.497
CLV_PCSK_SKI1_1	185	189	PF00082	0.420
CLV_PCSK_SKI1_1	334	338	PF00082	0.467
CLV_PCSK_SKI1_1	389	393	PF00082	0.342
CLV_PCSK_SKI1_1	43	47	PF00082	0.324
DEG_Nend_UBRbox_1	1	4	PF02207	0.418
DEG_SPOP_SBC_1	4	8	PF00917	0.405
DOC_CKS1_1	217	222	PF01111	0.442
DOC_CYCLIN_yCln2_LP_2	452	458	PF00134	0.392
DOC_MAPK_gen_1	192	200	PF00069	0.331
DOC_MAPK_gen_1	500	508	PF00069	0.428
DOC_MAPK_MEF2A_6	173	182	PF00069	0.371
DOC_MAPK_MEF2A_6	500	508	PF00069	0.363
DOC_MAPK_MEF2A_6	90	98	PF00069	0.412
DOC_MAPK_NFAT4_5	173	181	PF00069	0.435
DOC_PP4_FxxP_1	501	504	PF00568	0.356
DOC_USP7_MATH_1	103	107	PF00917	0.430
DOC_USP7_MATH_1	124	128	PF00917	0.443
DOC_USP7_MATH_1	4	8	PF00917	0.499
DOC_USP7_MATH_1	423	427	PF00917	0.467
DOC_USP7_MATH_1	484	488	PF00917	0.338
DOC_USP7_MATH_1	511	515	PF00917	0.437
DOC_USP7_MATH_1	85	89	PF00917	0.273
DOC_USP7_UBL2_3	165	169	PF12436	0.504
DOC_USP7_UBL2_3	192	196	PF12436	0.294
DOC_WW_Pin1_4	216	221	PF00397	0.392
DOC_WW_Pin1_4	466	471	PF00397	0.395
DOC_WW_Pin1_4	477	482	PF00397	0.384
LIG_14-3-3_CanoR_1	185	193	PF00244	0.441
LIG_14-3-3_CanoR_1	194	199	PF00244	0.451
LIG_14-3-3_CanoR_1	3	10	PF00244	0.493
LIG_BIR_III_4	385	389	PF00653	0.342
LIG_BRCT_BRCA1_1	27	31	PF00533	0.270
LIG_BRCT_BRCA1_1	497	501	PF00533	0.354
LIG_BRCT_BRCA1_1	59	63	PF00533	0.339
LIG_CtBP_PxDLS_1	249	255	PF00389	0.369
LIG_FHA_1	175	181	PF00498	0.448
LIG_FHA_1	350	356	PF00498	0.378
LIG_FHA_1	442	448	PF00498	0.323
LIG_FHA_2	28	34	PF00498	0.325
LIG_FHA_2	382	388	PF00498	0.342
LIG_FHA_2	81	87	PF00498	0.489
LIG_HP1_1	42	46	PF01393	0.190
LIG_LIR_Apic_2	275	281	PF02991	0.467
LIG_LIR_Apic_2	464	470	PF02991	0.377
LIG_LIR_Apic_2	498	504	PF02991	0.355
LIG_LIR_Gen_1	135	144	PF02991	0.309
LIG_LIR_Gen_1	226	237	PF02991	0.322
LIG_LIR_Gen_1	28	37	PF02991	0.480
LIG_LIR_Gen_1	341	351	PF02991	0.464
LIG_LIR_Nem_3	135	140	PF02991	0.315
LIG_LIR_Nem_3	226	232	PF02991	0.322
LIG_LIR_Nem_3	254	260	PF02991	0.351
LIG_LIR_Nem_3	28	34	PF02991	0.427
LIG_LIR_Nem_3	341	346	PF02991	0.464
LIG_LIR_Nem_3	451	457	PF02991	0.342
LIG_LYPXL_yS_3	454	457	PF13949	0.356
LIG_Pex14_2	501	505	PF04695	0.426
LIG_Pex14_3	527	532	PF04695	0.296
LIG_SH2_CRK	257	261	PF00017	0.351
LIG_SH2_CRK	278	282	PF00017	0.467
LIG_SH2_CRK	467	471	PF00017	0.388
LIG_SH2_NCK_1	229	233	PF00017	0.442
LIG_SH2_PTP2	137	140	PF00017	0.190
LIG_SH2_SRC	229	232	PF00017	0.290
LIG_SH2_STAP1	160	164	PF00017	0.497
LIG_SH2_STAP1	229	233	PF00017	0.341
LIG_SH2_STAP1	236	240	PF00017	0.284
LIG_SH2_STAT3	171	174	PF00017	0.516
LIG_SH2_STAT5	137	140	PF00017	0.380
LIG_SH2_STAT5	160	163	PF00017	0.417
LIG_SH2_STAT5	18	21	PF00017	0.542
LIG_SH2_STAT5	432	435	PF00017	0.363
LIG_SH2_STAT5	458	461	PF00017	0.373
LIG_SH2_STAT5	491	494	PF00017	0.360
LIG_SH2_STAT5	64	67	PF00017	0.357
LIG_SH3_3	135	141	PF00018	0.433
LIG_SH3_3	214	220	PF00018	0.322
LIG_Sin3_3	329	336	PF02671	0.467
LIG_SUMO_SIM_anti_2	368	375	PF11976	0.395
LIG_SUMO_SIM_anti_2	378	385	PF11976	0.395
LIG_TRAF2_1	127	130	PF00917	0.342
LIG_TRAF2_1	9	12	PF00917	0.504
LIG_TYR_ITIM	227	232	PF00017	0.442
LIG_TYR_ITIM	452	457	PF00017	0.356
MOD_CK1_1	13	19	PF00069	0.416
MOD_CK1_1	27	33	PF00069	0.440
MOD_CK1_1	35	41	PF00069	0.474
MOD_CK1_1	375	381	PF00069	0.351
MOD_CK1_1	480	486	PF00069	0.441
MOD_CK1_1	57	63	PF00069	0.392
MOD_CK2_1	113	119	PF00069	0.466
MOD_CK2_1	124	130	PF00069	0.401
MOD_CK2_1	381	387	PF00069	0.342
MOD_CK2_1	431	437	PF00069	0.462
MOD_CK2_1	5	11	PF00069	0.475
MOD_CK2_1	80	86	PF00069	0.441
MOD_GlcNHglycan	11	15	PF01048	0.432
MOD_GlcNHglycan	233	236	PF01048	0.368
MOD_GlcNHglycan	321	324	PF01048	0.451
MOD_GlcNHglycan	374	377	PF01048	0.351
MOD_GlcNHglycan	448	451	PF01048	0.353
MOD_GlcNHglycan	482	485	PF01048	0.463
MOD_GlcNHglycan	65	68	PF01048	0.255
MOD_GlcNHglycan	7	10	PF01048	0.507
MOD_GlcNHglycan	86	90	PF01048	0.304
MOD_GSK3_1	101	108	PF00069	0.417
MOD_GSK3_1	115	122	PF00069	0.284
MOD_GSK3_1	20	27	PF00069	0.387
MOD_GSK3_1	310	317	PF00069	0.396
MOD_GSK3_1	479	486	PF00069	0.407
MOD_N-GLC_1	281	286	PF02516	0.198
MOD_N-GLC_1	347	352	PF02516	0.449
MOD_NEK2_1	223	228	PF00069	0.331
MOD_NEK2_1	25	30	PF00069	0.338
MOD_NEK2_1	319	324	PF00069	0.458
MOD_NEK2_1	63	68	PF00069	0.464
MOD_NEK2_2	77	82	PF00069	0.420
MOD_PIKK_1	186	192	PF00454	0.508
MOD_PK_1	113	119	PF00069	0.402
MOD_PK_1	194	200	PF00069	0.327
MOD_PKA_2	4	10	PF00069	0.365
MOD_PKB_1	3	11	PF00069	0.483
MOD_Plk_1	10	16	PF00069	0.457
MOD_Plk_1	174	180	PF00069	0.516
MOD_Plk_1	281	287	PF00069	0.198
MOD_Plk_1	347	353	PF00069	0.461
MOD_Plk_1	54	60	PF00069	0.402
MOD_Plk_1	94	100	PF00069	0.420
MOD_Plk_4	223	229	PF00069	0.436
MOD_ProDKin_1	216	222	PF00069	0.392
MOD_ProDKin_1	466	472	PF00069	0.395
MOD_ProDKin_1	477	483	PF00069	0.384
MOD_SUMO_for_1	172	175	PF00179	0.266
MOD_SUMO_for_1	247	250	PF00179	0.431
TRG_DiLeu_BaEn_4	129	135	PF01217	0.256
TRG_ENDOCYTIC_2	137	140	PF00928	0.190
TRG_ENDOCYTIC_2	229	232	PF00928	0.442
TRG_ENDOCYTIC_2	257	260	PF00928	0.342
TRG_ENDOCYTIC_2	454	457	PF00928	0.366
TRG_NES_CRM1_1	202	214	PF08389	0.460
TRG_NLS_MonoCore_2	1	6	PF00514	0.415
TRG_Pf-PMV_PEXEL_1	82	86	PF00026	0.382

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8E6	Leptomonas seymouri	43%	100%
A0A0N1PAS4	Leptomonas seymouri	76%	99%
A0A0S4IQ79	Bodo saltans	42%	100%
A0A1X0NWR5	Trypanosomatidae	51%	100%
A0A1X0P4M9	Trypanosomatidae	38%	100%
A0A3R7JY84	Trypanosoma rangeli	51%	100%
A0A3R7N130	Trypanosoma rangeli	41%	96%
A0A3S7WRP1	Leishmania donovani	90%	100%
A0A3S7X742	Leishmania donovani	43%	100%
A1K9L5	Azoarcus sp. (strain BH72)	32%	100%
A1VP99	Polaromonas naphthalenivorans (strain CJ2)	29%	100%
A2SH61	Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1)	32%	100%
A4H6I8	Leishmania braziliensis	84%	100%
A4HCW4	Leishmania braziliensis	34%	100%
A4HLW7	Leishmania braziliensis	44%	97%
A4HUX3	Leishmania infantum	90%	100%
A4I996	Leishmania infantum	43%	100%
A6X259	Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37)	30%	100%
A9IIK3	Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)	32%	100%
B1LVC3	Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)	30%	100%
B1V932	Phytoplasma australiense	32%	100%
B2FMS4	Stenotrophomonas maltophilia (strain K279a)	30%	100%
B4SJ73	Stenotrophomonas maltophilia (strain R551-3)	30%	100%
B5EKZ2	Acidithiobacillus ferrooxidans (strain ATCC 53993 / BNL-5-31)	31%	100%
B7J5I9	Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455)	31%	100%
B8D7Q4	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)	30%	100%
B8D9F2	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)	30%	100%
B8HTK3	Cyanothece sp. (strain PCC 7425 / ATCC 29141)	27%	100%
C1F4B7	Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / BCRC 80197 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)	31%	100%
C3K6G5	Pseudomonas fluorescens (strain SBW25)	33%	100%
C3M9C8	Sinorhizobium fredii (strain NBRC 101917 / NGR234)	30%	100%
C4K346	Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT)	31%	100%
C4LA51	Tolumonas auensis (strain DSM 9187 / TA4)	30%	100%
C5CCM4	Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230)	34%	100%
C6E543	Geobacter sp. (strain M21)	33%	100%
D0A654	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	95%
D0A7H3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9B496	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	100%
P28838	Homo sapiens	27%	100%
P57448	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	30%	100%
P57823	Pasteurella multocida (strain Pm70)	32%	100%
Q0SQ50	Clostridium perfringens (strain SM101 / Type A)	26%	100%
Q1LJJ6	Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)	31%	100%
Q2KWX0	Bordetella avium (strain 197N)	30%	100%
Q2YB18	Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)	31%	100%
Q315M7	Oleidesulfovibrio alaskensis (strain ATCC BAA-1058 / DSM 17464 / G20)	28%	100%
Q3A831	Syntrophotalea carbinolica (strain DSM 2380 / NBRC 103641 / GraBd1)	32%	100%
Q46XT9	Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197)	32%	100%
Q4Q3T0	Leishmania major	43%	97%
Q4QH17	Leishmania major	89%	100%
Q5FFZ5	Ehrlichia ruminantium (strain Gardel)	30%	100%
Q5HAP2	Ehrlichia ruminantium (strain Welgevonden)	30%	100%
Q5QY05	Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)	29%	100%
Q5R7G6	Pongo abelii	37%	100%
Q68XM6	Rickettsia typhi (strain ATCC VR-144 / Wilmington)	32%	100%
Q6NSR8	Mus musculus	39%	100%
Q73QZ3	Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104)	30%	100%
Q73YK2	Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)	31%	100%
Q7M8W6	Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)	33%	100%
Q7W5K6	Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)	31%	100%
Q89AG2	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	28%	100%
Q8D295	Wigglesworthia glossinidia brevipalpis	28%	100%
Q8K9I0	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	30%	100%
Q8NDH3	Homo sapiens	37%	100%
Q8SQZ7	Encephalitozoon cuniculi (strain GB-M1)	29%	100%
Q8XHI3	Clostridium perfringens (strain 13 / Type A)	27%	100%
Q984S1	Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)	30%	100%
Q9JTI8	Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491)	32%	100%
Q9KTX5	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)	31%	100%
V5BTR1	Trypanosoma cruzi	41%	96%
V5BWE3	Trypanosoma cruzi	49%	100%