tRNA pseudouridine(55) synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9ANJ9_LEIMU

TriTrypDb:

LmxM.11.0530

Length:

721

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9ANJ9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ANJ9

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	94	98	PF00656	0.714
CLV_NRD_NRD_1	427	429	PF00675	0.409
CLV_NRD_NRD_1	563	565	PF00675	0.528
CLV_NRD_NRD_1	583	585	PF00675	0.237
CLV_NRD_NRD_1	645	647	PF00675	0.501
CLV_NRD_NRD_1	650	652	PF00675	0.399
CLV_NRD_NRD_1	82	84	PF00675	0.654
CLV_PCSK_KEX2_1	288	290	PF00082	0.686
CLV_PCSK_KEX2_1	563	565	PF00082	0.500
CLV_PCSK_KEX2_1	583	585	PF00082	0.237
CLV_PCSK_KEX2_1	602	604	PF00082	0.230
CLV_PCSK_KEX2_1	645	647	PF00082	0.501
CLV_PCSK_KEX2_1	650	652	PF00082	0.399
CLV_PCSK_KEX2_1	82	84	PF00082	0.674
CLV_PCSK_PC1ET2_1	288	290	PF00082	0.694
CLV_PCSK_PC1ET2_1	602	604	PF00082	0.346
CLV_PCSK_PC7_1	641	647	PF00082	0.482
CLV_PCSK_SKI1_1	17	21	PF00082	0.444
CLV_PCSK_SKI1_1	179	183	PF00082	0.638
CLV_PCSK_SKI1_1	317	321	PF00082	0.752
CLV_PCSK_SKI1_1	86	90	PF00082	0.647
DEG_APCC_DBOX_1	316	324	PF00400	0.752
DEG_MDM2_SWIB_1	181	188	PF02201	0.606
DEG_SCF_FBW7_1	658	665	PF00400	0.468
DEG_SPOP_SBC_1	302	306	PF00917	0.728
DEG_SPOP_SBC_1	512	516	PF00917	0.418
DOC_ANK_TNKS_1	536	543	PF00023	0.520
DOC_CKS1_1	636	641	PF01111	0.425
DOC_CYCLIN_yCln2_LP_2	99	105	PF00134	0.458
DOC_MAPK_DCC_7	251	260	PF00069	0.685
DOC_MAPK_FxFP_2	32	35	PF00069	0.628
DOC_MAPK_FxFP_2	462	465	PF00069	0.417
DOC_MAPK_gen_1	583	591	PF00069	0.534
DOC_MAPK_gen_1	650	660	PF00069	0.428
DOC_MAPK_gen_1	700	708	PF00069	0.368
DOC_MAPK_JIP1_4	543	549	PF00069	0.369
DOC_MAPK_MEF2A_6	251	260	PF00069	0.686
DOC_MAPK_MEF2A_6	531	538	PF00069	0.409
DOC_MAPK_MEF2A_6	584	593	PF00069	0.512
DOC_MAPK_MEF2A_6	690	697	PF00069	0.486
DOC_MAPK_MEF2A_6	702	710	PF00069	0.386
DOC_PP1_RVXF_1	603	610	PF00149	0.376
DOC_PP2B_LxvP_1	99	102	PF13499	0.744
DOC_PP4_FxxP_1	32	35	PF00568	0.593
DOC_PP4_FxxP_1	462	465	PF00568	0.402
DOC_USP7_MATH_1	115	119	PF00917	0.772
DOC_USP7_MATH_1	203	207	PF00917	0.673
DOC_USP7_MATH_1	240	244	PF00917	0.712
DOC_USP7_MATH_1	296	300	PF00917	0.703
DOC_USP7_MATH_1	302	306	PF00917	0.735
DOC_USP7_MATH_1	310	314	PF00917	0.605
DOC_USP7_MATH_1	331	335	PF00917	0.644
DOC_USP7_MATH_1	410	414	PF00917	0.650
DOC_USP7_MATH_1	474	478	PF00917	0.513
DOC_USP7_MATH_1	594	598	PF00917	0.484
DOC_WW_Pin1_4	160	165	PF00397	0.564
DOC_WW_Pin1_4	242	247	PF00397	0.533
DOC_WW_Pin1_4	434	439	PF00397	0.404
DOC_WW_Pin1_4	548	553	PF00397	0.422
DOC_WW_Pin1_4	635	640	PF00397	0.418
DOC_WW_Pin1_4	658	663	PF00397	0.453
DOC_WW_Pin1_4	689	694	PF00397	0.469
DOC_WW_Pin1_4	74	79	PF00397	0.594
LIG_14-3-3_CanoR_1	179	184	PF00244	0.554
LIG_14-3-3_CanoR_1	251	258	PF00244	0.703
LIG_14-3-3_CanoR_1	27	31	PF00244	0.542
LIG_14-3-3_CanoR_1	289	298	PF00244	0.669
LIG_14-3-3_CanoR_1	342	350	PF00244	0.615
LIG_14-3-3_CanoR_1	363	370	PF00244	0.640
LIG_14-3-3_CanoR_1	407	413	PF00244	0.654
LIG_14-3-3_CanoR_1	432	441	PF00244	0.502
LIG_14-3-3_CanoR_1	498	508	PF00244	0.603
LIG_14-3-3_CanoR_1	556	562	PF00244	0.483
LIG_14-3-3_CanoR_1	583	591	PF00244	0.442
LIG_14-3-3_CanoR_1	595	601	PF00244	0.445
LIG_14-3-3_CanoR_1	645	649	PF00244	0.494
LIG_14-3-3_CanoR_1	653	659	PF00244	0.399
LIG_APCC_ABBA_1	258	263	PF00400	0.672
LIG_APCC_ABBAyCdc20_2	681	687	PF00400	0.393
LIG_BIR_II_1	1	5	PF00653	0.727
LIG_BRCT_BRCA1_1	28	32	PF00533	0.552
LIG_BRCT_BRCA1_1	436	440	PF00533	0.418
LIG_Clathr_ClatBox_1	669	673	PF01394	0.405
LIG_eIF4E_1	145	151	PF01652	0.625
LIG_FHA_1	170	176	PF00498	0.671
LIG_FHA_1	250	256	PF00498	0.715
LIG_FHA_1	293	299	PF00498	0.727
LIG_FHA_1	344	350	PF00498	0.612
LIG_FHA_1	363	369	PF00498	0.560
LIG_FHA_1	588	594	PF00498	0.468
LIG_FHA_1	609	615	PF00498	0.407
LIG_FHA_1	690	696	PF00498	0.435
LIG_FHA_1	701	707	PF00498	0.346
LIG_FHA_2	196	202	PF00498	0.682
LIG_FHA_2	266	272	PF00498	0.664
LIG_FHA_2	279	285	PF00498	0.595
LIG_FHA_2	391	397	PF00498	0.447
LIG_FHA_2	480	486	PF00498	0.673
LIG_FHA_2	556	562	PF00498	0.470
LIG_FHA_2	583	589	PF00498	0.448
LIG_FHA_2	60	66	PF00498	0.593
LIG_FHA_2	92	98	PF00498	0.696
LIG_LIR_Apic_2	29	35	PF02991	0.578
LIG_LIR_Apic_2	375	379	PF02991	0.484
LIG_LIR_Gen_1	135	144	PF02991	0.610
LIG_LIR_Gen_1	180	189	PF02991	0.486
LIG_LIR_Gen_1	502	513	PF02991	0.670
LIG_LIR_Nem_3	135	139	PF02991	0.615
LIG_LIR_Nem_3	180	186	PF02991	0.496
LIG_LIR_Nem_3	190	195	PF02991	0.486
LIG_LIR_Nem_3	214	219	PF02991	0.615
LIG_LIR_Nem_3	502	508	PF02991	0.696
LIG_LIR_Nem_3	665	670	PF02991	0.490
LIG_MYND_1	468	472	PF01753	0.590
LIG_NRBOX	694	700	PF00104	0.399
LIG_Pex14_2	181	185	PF04695	0.605
LIG_Rb_pABgroove_1	26	34	PF01858	0.619
LIG_SH2_CRK	370	374	PF00017	0.487
LIG_SH2_CRK	376	380	PF00017	0.481
LIG_SH2_CRK	604	608	PF00017	0.371
LIG_SH2_NCK_1	370	374	PF00017	0.522
LIG_SH2_NCK_1	376	380	PF00017	0.515
LIG_SH2_SRC	374	377	PF00017	0.491
LIG_SH2_STAP1	132	136	PF00017	0.648
LIG_SH2_STAP1	166	170	PF00017	0.707
LIG_SH2_STAP1	370	374	PF00017	0.503
LIG_SH2_STAT5	192	195	PF00017	0.606
LIG_SH2_STAT5	231	234	PF00017	0.556
LIG_SH2_STAT5	261	264	PF00017	0.592
LIG_SH2_STAT5	359	362	PF00017	0.561
LIG_SH2_STAT5	380	383	PF00017	0.496
LIG_SH2_STAT5	422	425	PF00017	0.468
LIG_SH2_STAT5	679	682	PF00017	0.387
LIG_SH2_STAT5	84	87	PF00017	0.655
LIG_SH3_3	455	461	PF00018	0.424
LIG_SH3_3	462	468	PF00018	0.485
LIG_SUMO_SIM_par_1	492	497	PF11976	0.701
LIG_SUMO_SIM_par_1	545	551	PF11976	0.311
LIG_SUMO_SIM_par_1	668	674	PF11976	0.449
LIG_TRAF2_1	276	279	PF00917	0.502
LIG_TRAF2_1	394	397	PF00917	0.669
LIG_TRAF2_1	450	453	PF00917	0.366
LIG_TYR_ITIM	368	373	PF00017	0.491
LIG_WRC_WIRS_1	446	451	PF05994	0.496
LIG_WW_3	35	39	PF00397	0.595
MOD_CDC14_SPxK_1	551	554	PF00782	0.446
MOD_CDK_SPK_2	74	79	PF00069	0.564
MOD_CDK_SPxK_1	548	554	PF00069	0.429
MOD_CDK_SPxK_1	635	641	PF00069	0.424
MOD_CK1_1	242	248	PF00069	0.644
MOD_CK1_1	250	256	PF00069	0.636
MOD_CK1_1	303	309	PF00069	0.747
MOD_CK1_1	313	319	PF00069	0.570
MOD_CK1_1	329	335	PF00069	0.623
MOD_CK1_1	46	52	PF00069	0.691
MOD_CK1_1	511	517	PF00069	0.584
MOD_CK1_1	53	59	PF00069	0.650
MOD_CK2_1	195	201	PF00069	0.649
MOD_CK2_1	278	284	PF00069	0.602
MOD_CK2_1	390	396	PF00069	0.710
MOD_CK2_1	447	453	PF00069	0.397
MOD_CK2_1	479	485	PF00069	0.643
MOD_CK2_1	568	574	PF00069	0.469
MOD_GlcNHglycan	1	4	PF01048	0.747
MOD_GlcNHglycan	298	301	PF01048	0.688
MOD_GlcNHglycan	312	315	PF01048	0.581
MOD_GlcNHglycan	328	331	PF01048	0.710
MOD_GlcNHglycan	48	51	PF01048	0.684
MOD_GlcNHglycan	482	485	PF01048	0.732
MOD_GlcNHglycan	489	492	PF01048	0.741
MOD_GlcNHglycan	52	55	PF01048	0.686
MOD_GlcNHglycan	527	530	PF01048	0.392
MOD_GlcNHglycan	79	82	PF01048	0.510
MOD_GlcNHglycan	9	12	PF01048	0.669
MOD_GSK3_1	111	118	PF00069	0.750
MOD_GSK3_1	165	172	PF00069	0.577
MOD_GSK3_1	177	184	PF00069	0.523
MOD_GSK3_1	261	268	PF00069	0.543
MOD_GSK3_1	292	299	PF00069	0.680
MOD_GSK3_1	42	49	PF00069	0.614
MOD_GSK3_1	475	482	PF00069	0.544
MOD_GSK3_1	508	515	PF00069	0.623
MOD_GSK3_1	569	576	PF00069	0.433
MOD_GSK3_1	654	661	PF00069	0.428
MOD_GSK3_1	671	678	PF00069	0.378
MOD_N-GLC_1	177	182	PF02516	0.606
MOD_N-GLC_1	361	366	PF02516	0.630
MOD_N-GLC_1	432	437	PF02516	0.524
MOD_NEK2_1	181	186	PF00069	0.558
MOD_NEK2_1	195	200	PF00069	0.570
MOD_NEK2_1	26	31	PF00069	0.473
MOD_NEK2_1	301	306	PF00069	0.644
MOD_NEK2_1	326	331	PF00069	0.684
MOD_NEK2_1	381	386	PF00069	0.457
MOD_NEK2_1	416	421	PF00069	0.422
MOD_NEK2_1	423	428	PF00069	0.363
MOD_NEK2_1	43	48	PF00069	0.489
MOD_NEK2_1	654	659	PF00069	0.432
MOD_NEK2_1	688	693	PF00069	0.441
MOD_NEK2_2	587	592	PF00069	0.486
MOD_PIKK_1	250	256	PF00454	0.643
MOD_PIKK_1	432	438	PF00454	0.404
MOD_PIKK_1	56	62	PF00454	0.392
MOD_PIKK_1	671	677	PF00454	0.369
MOD_PKA_2	250	256	PF00069	0.694
MOD_PKA_2	26	32	PF00069	0.458
MOD_PKA_2	326	332	PF00069	0.677
MOD_PKA_2	362	368	PF00069	0.652
MOD_PKA_2	402	408	PF00069	0.654
MOD_PKA_2	555	561	PF00069	0.550
MOD_PKA_2	582	588	PF00069	0.433
MOD_PKA_2	594	600	PF00069	0.458
MOD_PKA_2	644	650	PF00069	0.495
MOD_Plk_1	177	183	PF00069	0.598
MOD_Plk_1	265	271	PF00069	0.440
MOD_Plk_1	292	298	PF00069	0.706
MOD_Plk_1	475	481	PF00069	0.561
MOD_Plk_1	587	593	PF00069	0.460
MOD_Plk_2-3	555	561	PF00069	0.514
MOD_Plk_4	132	138	PF00069	0.622
MOD_Plk_4	204	210	PF00069	0.644
MOD_Plk_4	26	32	PF00069	0.458
MOD_Plk_4	331	337	PF00069	0.646
MOD_Plk_4	368	374	PF00069	0.518
MOD_Plk_4	513	519	PF00069	0.392
MOD_Plk_4	675	681	PF00069	0.391
MOD_ProDKin_1	160	166	PF00069	0.564
MOD_ProDKin_1	242	248	PF00069	0.533
MOD_ProDKin_1	434	440	PF00069	0.406
MOD_ProDKin_1	548	554	PF00069	0.429
MOD_ProDKin_1	635	641	PF00069	0.424
MOD_ProDKin_1	658	664	PF00069	0.455
MOD_ProDKin_1	689	695	PF00069	0.464
MOD_ProDKin_1	74	80	PF00069	0.588
MOD_SUMO_for_1	209	212	PF00179	0.683
MOD_SUMO_rev_2	597	601	PF00179	0.548
TRG_DiLeu_BaEn_2	540	546	PF01217	0.537
TRG_DiLeu_BaLyEn_6	455	460	PF01217	0.440
TRG_DiLeu_BaLyEn_6	650	655	PF01217	0.546
TRG_ENDOCYTIC_2	145	148	PF00928	0.496
TRG_ENDOCYTIC_2	192	195	PF00928	0.606
TRG_ENDOCYTIC_2	370	373	PF00928	0.486
TRG_ENDOCYTIC_2	427	430	PF00928	0.382
TRG_ENDOCYTIC_2	604	607	PF00928	0.377
TRG_ER_diArg_1	224	227	PF00400	0.503
TRG_ER_diArg_1	562	564	PF00400	0.497
TRG_ER_diArg_1	582	584	PF00400	0.230
TRG_ER_diArg_1	650	653	PF00400	0.442
TRG_ER_diArg_1	82	84	PF00400	0.670
TRG_NES_CRM1_1	211	223	PF08389	0.644
TRG_NES_CRM1_1	704	719	PF08389	0.425
TRG_NLS_MonoExtC_3	287	293	PF00514	0.654
TRG_NLS_MonoExtN_4	285	292	PF00514	0.710
TRG_NLS_MonoExtN_4	600	606	PF00514	0.342
TRG_Pf-PMV_PEXEL_1	537	541	PF00026	0.497
TRG_Pf-PMV_PEXEL_1	612	616	PF00026	0.444
TRG_Pf-PMV_PEXEL_1	630	635	PF00026	0.533

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW55	Leptomonas seymouri	68%	96%
A0A0S4IV39	Bodo saltans	36%	90%
A0A1X0NVE2	Trypanosomatidae	45%	100%
A0A3S5H6I6	Leishmania donovani	92%	100%
A0A422MV99	Trypanosoma rangeli	44%	100%
A4H6H8	Leishmania braziliensis	83%	100%
A4HUW4	Leishmania infantum	92%	100%
D0A7I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
Q4QH27	Leishmania major	93%	100%
V5BW97	Trypanosoma cruzi	45%	100%