Putative DEAD-boc ATP-dependent (RNA) helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DEAD-boc ATP-dependent (RNA) helicase, putative

Species:

Leishmania mexicana

UniProt:

E9ANG5_LEIMU

TriTrypDb:

LmxM.11.0190

Length:

609

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005681	spliceosomal complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0071013	catalytic step 2 spliceosome	3	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990904	ribonucleoprotein complex	2	1
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ANG5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ANG5

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	1
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	1
GO:0000398	mRNA splicing, via spliceosome	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0008380	RNA splicing	7	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003723	RNA binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	255	257	PF00675	0.209
CLV_NRD_NRD_1	294	296	PF00675	0.276
CLV_NRD_NRD_1	401	403	PF00675	0.489
CLV_NRD_NRD_1	7	9	PF00675	0.575
CLV_PCSK_FUR_1	292	296	PF00082	0.276
CLV_PCSK_KEX2_1	123	125	PF00082	0.474
CLV_PCSK_KEX2_1	255	257	PF00082	0.209
CLV_PCSK_KEX2_1	294	296	PF00082	0.269
CLV_PCSK_KEX2_1	401	403	PF00082	0.470
CLV_PCSK_KEX2_1	460	462	PF00082	0.618
CLV_PCSK_KEX2_1	6	8	PF00082	0.557
CLV_PCSK_PC1ET2_1	123	125	PF00082	0.515
CLV_PCSK_PC1ET2_1	460	462	PF00082	0.624
CLV_PCSK_PC7_1	3	9	PF00082	0.447
CLV_PCSK_SKI1_1	357	361	PF00082	0.455
CLV_PCSK_SKI1_1	374	378	PF00082	0.277
CLV_PCSK_SKI1_1	7	11	PF00082	0.486
DEG_APCC_DBOX_1	356	364	PF00400	0.310
DEG_APCC_DBOX_1	6	14	PF00400	0.565
DEG_Nend_UBRbox_3	1	3	PF02207	0.578
DOC_ANK_TNKS_1	400	407	PF00023	0.530
DOC_ANK_TNKS_1	539	546	PF00023	0.543
DOC_CKS1_1	233	238	PF01111	0.420
DOC_CKS1_1	436	441	PF01111	0.457
DOC_CYCLIN_RxL_1	184	194	PF00134	0.338
DOC_CYCLIN_RxL_1	351	362	PF00134	0.429
DOC_CYCLIN_yClb5_NLxxxL_5	281	290	PF00134	0.451
DOC_CYCLIN_yCln2_LP_2	114	120	PF00134	0.469
DOC_MAPK_DCC_7	8	18	PF00069	0.402
DOC_MAPK_gen_1	253	263	PF00069	0.409
DOC_MAPK_gen_1	6	18	PF00069	0.412
DOC_MAPK_MEF2A_6	11	18	PF00069	0.403
DOC_MAPK_MEF2A_6	110	118	PF00069	0.284
DOC_MAPK_MEF2A_6	157	165	PF00069	0.513
DOC_MAPK_MEF2A_6	357	365	PF00069	0.318
DOC_PP1_RVXF_1	372	379	PF00149	0.328
DOC_PP2B_LxvP_1	114	117	PF13499	0.475
DOC_PP2B_LxvP_1	550	553	PF13499	0.349
DOC_PP2B_LxvP_1	566	569	PF13499	0.277
DOC_USP7_MATH_1	211	215	PF00917	0.451
DOC_USP7_MATH_1	455	459	PF00917	0.539
DOC_USP7_MATH_1	524	528	PF00917	0.489
DOC_USP7_MATH_1	569	573	PF00917	0.478
DOC_USP7_MATH_1	60	64	PF00917	0.505
DOC_USP7_MATH_1	66	70	PF00917	0.483
DOC_USP7_UBL2_3	253	257	PF12436	0.404
DOC_WW_Pin1_4	105	110	PF00397	0.421
DOC_WW_Pin1_4	232	237	PF00397	0.420
DOC_WW_Pin1_4	243	248	PF00397	0.420
DOC_WW_Pin1_4	406	411	PF00397	0.418
DOC_WW_Pin1_4	435	440	PF00397	0.537
DOC_WW_Pin1_4	58	63	PF00397	0.442
LIG_14-3-3_CanoR_1	129	137	PF00244	0.259
LIG_14-3-3_CanoR_1	177	182	PF00244	0.409
LIG_14-3-3_CanoR_1	328	334	PF00244	0.376
LIG_14-3-3_CanoR_1	574	582	PF00244	0.500
LIG_14-3-3_CanoR_1	588	596	PF00244	0.668
LIG_APCC_ABBA_1	375	380	PF00400	0.396
LIG_APCC_ABBAyCdc20_2	374	380	PF00400	0.401
LIG_BIR_III_2	59	63	PF00653	0.445
LIG_deltaCOP1_diTrp_1	194	200	PF00928	0.409
LIG_FHA_1	113	119	PF00498	0.448
LIG_FHA_1	178	184	PF00498	0.412
LIG_FHA_1	202	208	PF00498	0.432
LIG_FHA_1	324	330	PF00498	0.394
LIG_FHA_1	371	377	PF00498	0.507
LIG_FHA_1	515	521	PF00498	0.409
LIG_FHA_2	319	325	PF00498	0.549
LIG_FHA_2	377	383	PF00498	0.363
LIG_FHA_2	390	396	PF00498	0.394
LIG_LIR_LC3C_4	495	500	PF02991	0.513
LIG_LIR_Nem_3	341	347	PF02991	0.288
LIG_NBox_RRM_1	159	169	PF00076	0.409
LIG_PCNA_yPIPBox_3	168	182	PF02747	0.409
LIG_Pex14_1	196	200	PF04695	0.409
LIG_SH2_SRC	12	15	PF00017	0.416
LIG_SH2_SRC	467	470	PF00017	0.686
LIG_SH2_STAP1	193	197	PF00017	0.409
LIG_SH2_STAT3	103	106	PF00017	0.507
LIG_SH2_STAT3	276	279	PF00017	0.513
LIG_SH2_STAT3	31	34	PF00017	0.511
LIG_SH2_STAT5	12	15	PF00017	0.423
LIG_SH2_STAT5	31	34	PF00017	0.511
LIG_SH2_STAT5	345	348	PF00017	0.280
LIG_SH2_STAT5	429	432	PF00017	0.468
LIG_SH2_STAT5	483	486	PF00017	0.435
LIG_SH2_STAT5	50	53	PF00017	0.488
LIG_SH3_3	179	185	PF00018	0.513
LIG_SH3_3	230	236	PF00018	0.420
LIG_SH3_3	433	439	PF00018	0.781
LIG_SH3_3	546	552	PF00018	0.347
LIG_SUMO_SIM_anti_2	180	186	PF11976	0.420
LIG_SUMO_SIM_anti_2	546	551	PF11976	0.408
LIG_SUMO_SIM_par_1	180	186	PF11976	0.420
LIG_WRC_WIRS_1	502	507	PF05994	0.500
MOD_CDC14_SPxK_1	409	412	PF00782	0.496
MOD_CDK_SPK_2	105	110	PF00069	0.392
MOD_CDK_SPxK_1	232	238	PF00069	0.420
MOD_CDK_SPxK_1	406	412	PF00069	0.491
MOD_CK1_1	424	430	PF00069	0.607
MOD_CK1_1	488	494	PF00069	0.492
MOD_CK1_1	504	510	PF00069	0.364
MOD_CK1_1	573	579	PF00069	0.414
MOD_CK1_1	61	67	PF00069	0.651
MOD_CK1_1	88	94	PF00069	0.644
MOD_CK2_1	290	296	PF00069	0.344
MOD_CK2_1	329	335	PF00069	0.384
MOD_CK2_1	376	382	PF00069	0.367
MOD_CK2_1	389	395	PF00069	0.355
MOD_CK2_1	561	567	PF00069	0.524
MOD_CK2_1	573	579	PF00069	0.376
MOD_GlcNHglycan	22	25	PF01048	0.512
MOD_GlcNHglycan	281	284	PF01048	0.227
MOD_GlcNHglycan	305	308	PF01048	0.209
MOD_GlcNHglycan	392	395	PF01048	0.450
MOD_GlcNHglycan	426	429	PF01048	0.605
MOD_GlcNHglycan	457	460	PF01048	0.734
MOD_GlcNHglycan	462	465	PF01048	0.682
MOD_GlcNHglycan	526	529	PF01048	0.311
MOD_GlcNHglycan	554	557	PF01048	0.377
MOD_GlcNHglycan	575	578	PF01048	0.412
MOD_GlcNHglycan	583	586	PF01048	0.491
MOD_GlcNHglycan	591	594	PF01048	0.578
MOD_GlcNHglycan	599	602	PF01048	0.709
MOD_GlcNHglycan	63	66	PF01048	0.732
MOD_GSK3_1	148	155	PF00069	0.513
MOD_GSK3_1	323	330	PF00069	0.437
MOD_GSK3_1	334	341	PF00069	0.306
MOD_GSK3_1	386	393	PF00069	0.425
MOD_GSK3_1	451	458	PF00069	0.465
MOD_GSK3_1	501	508	PF00069	0.428
MOD_GSK3_1	569	576	PF00069	0.460
MOD_GSK3_1	599	606	PF00069	0.527
MOD_GSK3_1	84	91	PF00069	0.666
MOD_N-GLC_1	485	490	PF02516	0.252
MOD_N-GLC_1	597	602	PF02516	0.515
MOD_NEK2_1	27	32	PF00069	0.518
MOD_NEK2_1	290	295	PF00069	0.344
MOD_NEK2_1	329	334	PF00069	0.394
MOD_NEK2_1	347	352	PF00069	0.257
MOD_NEK2_1	359	364	PF00069	0.426
MOD_NEK2_1	365	370	PF00069	0.440
MOD_NEK2_1	505	510	PF00069	0.552
MOD_NEK2_1	570	575	PF00069	0.477
MOD_NEK2_1	597	602	PF00069	0.667
MOD_NEK2_1	84	89	PF00069	0.559
MOD_PIKK_1	202	208	PF00454	0.432
MOD_PIKK_1	365	371	PF00454	0.474
MOD_PIKK_1	44	50	PF00454	0.444
MOD_PIKK_1	448	454	PF00454	0.582
MOD_PIKK_1	66	72	PF00454	0.551
MOD_PIKK_1	85	91	PF00454	0.496
MOD_PKA_1	460	466	PF00069	0.455
MOD_PKA_2	128	134	PF00069	0.248
MOD_PKA_2	2	8	PF00069	0.562
MOD_PKA_2	279	285	PF00069	0.431
MOD_PKA_2	327	333	PF00069	0.372
MOD_PKA_2	460	466	PF00069	0.735
MOD_PKA_2	573	579	PF00069	0.488
MOD_PKA_2	587	593	PF00069	0.659
MOD_Plk_1	323	329	PF00069	0.441
MOD_Plk_1	334	340	PF00069	0.308
MOD_Plk_1	485	491	PF00069	0.410
MOD_Plk_4	177	183	PF00069	0.409
MOD_Plk_4	245	251	PF00069	0.409
MOD_Plk_4	27	33	PF00069	0.478
MOD_Plk_4	329	335	PF00069	0.417
MOD_Plk_4	386	392	PF00069	0.496
MOD_ProDKin_1	105	111	PF00069	0.422
MOD_ProDKin_1	232	238	PF00069	0.420
MOD_ProDKin_1	243	249	PF00069	0.420
MOD_ProDKin_1	406	412	PF00069	0.412
MOD_ProDKin_1	435	441	PF00069	0.538
MOD_ProDKin_1	58	64	PF00069	0.445
MOD_SUMO_rev_2	489	499	PF00179	0.513
TRG_DiLeu_BaEn_1	356	361	PF01217	0.357
TRG_DiLeu_BaEn_1	386	391	PF01217	0.434
TRG_DiLeu_BaEn_1	544	549	PF01217	0.394
TRG_DiLeu_BaLyEn_6	136	141	PF01217	0.476
TRG_DiLeu_BaLyEn_6	5	10	PF01217	0.564
TRG_ENDOCYTIC_2	467	470	PF00928	0.686
TRG_ENDOCYTIC_2	50	53	PF00928	0.548
TRG_ER_diArg_1	124	127	PF00400	0.462
TRG_ER_diArg_1	254	256	PF00400	0.408
TRG_ER_diArg_1	278	281	PF00400	0.452
TRG_ER_diArg_1	291	294	PF00400	0.393
TRG_ER_diArg_1	400	402	PF00400	0.472
TRG_ER_diArg_1	6	8	PF00400	0.570
TRG_NES_CRM1_1	172	188	PF08389	0.420
TRG_NES_CRM1_1	252	267	PF08389	0.409
TRG_Pf-PMV_PEXEL_1	168	172	PF00026	0.220

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJV4	Leptomonas seymouri	75%	100%
A0A0S4JYL8	Bodo saltans	49%	92%
A0A1X0NSB4	Trypanosomatidae	21%	100%
A0A1X0NV90	Trypanosomatidae	60%	100%
A0A3Q8ICA8	Leishmania donovani	94%	100%
A0A3R7MZZ8	Trypanosoma rangeli	60%	100%
A4H9T9	Leishmania braziliensis	88%	100%
A4HUT1	Leishmania infantum	94%	100%
D0A7N1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
D0AAP5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	100%
Q09719	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	72%
Q4P7M1	Ustilago maydis (strain 521 / FGSC 9021)	25%	89%
Q4QH61	Leishmania major	93%	100%
Q6FU81	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	23%	91%
Q8W4E1	Arabidopsis thaliana	23%	100%
V5C100	Trypanosoma cruzi	60%	99%