Putative translation initiation factor EIF-2B gamma subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor EIF-2B gamma subunit, putative

Species:

Leishmania mexicana

UniProt:

E9AMW0_LEIMU

TriTrypDb:

LmxM.09.1090

Length:

595

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005850	eukaryotic translation initiation factor 2 complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AMW0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMW0

Function

Biological processes
Term	Name	Level	Count
GO:0001731	formation of translation preinitiation complex	7	2
GO:0006417	regulation of translation	6	2
GO:0006450	regulation of translational fidelity	3	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0009891	positive regulation of biosynthetic process	5	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010557	positive regulation of macromolecule biosynthetic process	6	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0010628	positive regulation of gene expression	6	2
GO:0016043	cellular component organization	3	2
GO:0019222	regulation of metabolic process	3	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031325	positive regulation of cellular metabolic process	5	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0031328	positive regulation of cellular biosynthetic process	6	2
GO:0034248	regulation of amide metabolic process	5	2
GO:0034250	positive regulation of amide metabolic process	6	2
GO:0043933	protein-containing complex organization	4	2
GO:0045727	positive regulation of translation	7	2
GO:0045903	positive regulation of translational fidelity	4	2
GO:0048518	positive regulation of biological process	3	2
GO:0048522	positive regulation of cellular process	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051173	positive regulation of nitrogen compound metabolic process	5	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0051247	positive regulation of protein metabolic process	6	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065003	protein-containing complex assembly	5	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003676	nucleic acid binding	3	13
GO:0003743	translation initiation factor activity	4	13
GO:0003824	catalytic activity	1	13
GO:0003924	GTPase activity	7	13
GO:0005488	binding	1	13
GO:0005525	GTP binding	5	13
GO:0008135	translation factor activity, RNA binding	3	13
GO:0016462	pyrophosphatase activity	5	13
GO:0016787	hydrolase activity	2	13
GO:0016817	hydrolase activity, acting on acid anhydrides	3	13
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	13
GO:0017076	purine nucleotide binding	4	13
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	13
GO:0019001	guanyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032561	guanyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0045182	translation regulator activity	1	13
GO:0090079	translation regulator activity, nucleic acid binding	2	13
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13
GO:0000049	tRNA binding	5	7
GO:0003723	RNA binding	4	7
GO:0003746	translation elongation factor activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	114	118	PF00656	0.628
CLV_C14_Caspase3-7	424	428	PF00656	0.637
CLV_NRD_NRD_1	251	253	PF00675	0.511
CLV_NRD_NRD_1	312	314	PF00675	0.541
CLV_NRD_NRD_1	319	321	PF00675	0.499
CLV_NRD_NRD_1	393	395	PF00675	0.440
CLV_NRD_NRD_1	401	403	PF00675	0.567
CLV_NRD_NRD_1	512	514	PF00675	0.349
CLV_NRD_NRD_1	570	572	PF00675	0.345
CLV_PCSK_FUR_1	399	403	PF00082	0.519
CLV_PCSK_KEX2_1	131	133	PF00082	0.334
CLV_PCSK_KEX2_1	251	253	PF00082	0.511
CLV_PCSK_KEX2_1	311	313	PF00082	0.521
CLV_PCSK_KEX2_1	319	321	PF00082	0.491
CLV_PCSK_KEX2_1	393	395	PF00082	0.440
CLV_PCSK_KEX2_1	401	403	PF00082	0.567
CLV_PCSK_KEX2_1	512	514	PF00082	0.446
CLV_PCSK_PC1ET2_1	131	133	PF00082	0.334
CLV_PCSK_SKI1_1	279	283	PF00082	0.461
CLV_PCSK_SKI1_1	46	50	PF00082	0.454
CLV_PCSK_SKI1_1	469	473	PF00082	0.452
CLV_PCSK_SKI1_1	562	566	PF00082	0.390
DEG_SPOP_SBC_1	420	424	PF00917	0.629
DOC_CDC14_PxL_1	301	309	PF14671	0.405
DOC_CYCLIN_yCln2_LP_2	550	556	PF00134	0.349
DOC_MAPK_gen_1	383	391	PF00069	0.482
DOC_MAPK_gen_1	76	85	PF00069	0.534
DOC_MAPK_MEF2A_6	518	527	PF00069	0.390
DOC_MAPK_MEF2A_6	79	87	PF00069	0.534
DOC_MAPK_RevD_3	305	320	PF00069	0.499
DOC_PP1_RVXF_1	572	579	PF00149	0.358
DOC_PP2B_LxvP_1	474	477	PF13499	0.488
DOC_PP4_FxxP_1	169	172	PF00568	0.509
DOC_PP4_FxxP_1	361	364	PF00568	0.441
DOC_PP4_FxxP_1	437	440	PF00568	0.507
DOC_USP7_MATH_1	165	169	PF00917	0.572
DOC_USP7_MATH_1	414	418	PF00917	0.667
DOC_USP7_MATH_1	430	434	PF00917	0.515
DOC_WW_Pin1_4	116	121	PF00397	0.559
DOC_WW_Pin1_4	331	336	PF00397	0.630
DOC_WW_Pin1_4	403	408	PF00397	0.610
DOC_WW_Pin1_4	416	421	PF00397	0.564
DOC_WW_Pin1_4	426	431	PF00397	0.610
DOC_WW_Pin1_4	438	443	PF00397	0.547
DOC_WW_Pin1_4	480	485	PF00397	0.651
DOC_WW_Pin1_4	549	554	PF00397	0.380
LIG_14-3-3_CanoR_1	132	137	PF00244	0.509
LIG_14-3-3_CanoR_1	346	350	PF00244	0.442
LIG_14-3-3_CanoR_1	505	511	PF00244	0.524
LIG_Actin_WH2_2	454	471	PF00022	0.464
LIG_Actin_WH2_2	523	540	PF00022	0.364
LIG_Actin_WH2_2	569	585	PF00022	0.303
LIG_APCC_ABBA_1	455	460	PF00400	0.462
LIG_BIR_II_1	1	5	PF00653	0.718
LIG_BIR_III_2	427	431	PF00653	0.568
LIG_FHA_1	125	131	PF00498	0.628
LIG_FHA_1	345	351	PF00498	0.510
LIG_FHA_1	38	44	PF00498	0.538
LIG_FHA_1	416	422	PF00498	0.601
LIG_FHA_1	47	53	PF00498	0.400
LIG_FHA_1	84	90	PF00498	0.509
LIG_FHA_2	370	376	PF00498	0.444
LIG_FHA_2	422	428	PF00498	0.637
LIG_FHA_2	8	14	PF00498	0.689
LIG_LIR_Apic_2	167	172	PF02991	0.509
LIG_LIR_Apic_2	339	344	PF02991	0.624
LIG_LIR_Apic_2	358	364	PF02991	0.262
LIG_LIR_Gen_1	27	36	PF02991	0.594
LIG_LIR_Gen_1	39	48	PF02991	0.426
LIG_LIR_Nem_3	106	111	PF02991	0.628
LIG_LIR_Nem_3	27	31	PF02991	0.564
LIG_PCNA_PIPBox_1	207	216	PF02747	0.535
LIG_PCNA_PIPBox_1	459	468	PF02747	0.407
LIG_PCNA_yPIPBox_3	459	469	PF02747	0.398
LIG_REV1ctd_RIR_1	463	473	PF16727	0.468
LIG_SH2_CRK	208	212	PF00017	0.493
LIG_SH2_CRK	259	263	PF00017	0.357
LIG_SH2_CRK	341	345	PF00017	0.608
LIG_SH2_GRB2like	259	262	PF00017	0.406
LIG_SH2_GRB2like	91	94	PF00017	0.509
LIG_SH2_NCK_1	221	225	PF00017	0.503
LIG_SH2_SRC	458	461	PF00017	0.381
LIG_SH2_SRC	91	94	PF00017	0.509
LIG_SH2_STAT5	214	217	PF00017	0.572
LIG_SH2_STAT5	243	246	PF00017	0.511
LIG_SH2_STAT5	331	334	PF00017	0.603
LIG_SH2_STAT5	452	455	PF00017	0.631
LIG_SH2_STAT5	568	571	PF00017	0.349
LIG_SH3_3	134	140	PF00018	0.509
LIG_SH3_3	222	228	PF00018	0.534
LIG_SH3_3	242	248	PF00018	0.549
LIG_SH3_3	265	271	PF00018	0.502
LIG_SH3_3	329	335	PF00018	0.597
LIG_Sin3_3	158	165	PF02671	0.509
LIG_SUMO_SIM_anti_2	187	193	PF11976	0.502
LIG_SUMO_SIM_anti_2	299	305	PF11976	0.428
LIG_SUMO_SIM_par_1	366	373	PF11976	0.420
LIG_SUMO_SIM_par_1	519	529	PF11976	0.345
LIG_UBA3_1	189	195	PF00899	0.553
LIG_WRC_WIRS_1	263	268	PF05994	0.439
LIG_WRC_WIRS_1	38	43	PF05994	0.556
MOD_CDC14_SPxK_1	483	486	PF00782	0.523
MOD_CDK_SPxK_1	480	486	PF00069	0.563
MOD_CK1_1	415	421	PF00069	0.633
MOD_CK1_1	426	432	PF00069	0.568
MOD_CK1_1	441	447	PF00069	0.491
MOD_CK1_1	526	532	PF00069	0.349
MOD_CK1_1	552	558	PF00069	0.303
MOD_CK2_1	322	328	PF00069	0.619
MOD_GlcNHglycan	324	327	PF01048	0.626
MOD_GlcNHglycan	414	417	PF01048	0.662
MOD_GlcNHglycan	432	435	PF01048	0.505
MOD_GlcNHglycan	443	446	PF01048	0.535
MOD_GlcNHglycan	478	481	PF01048	0.562
MOD_GlcNHglycan	554	557	PF01048	0.349
MOD_GlcNHglycan	69	72	PF01048	0.334
MOD_GSK3_1	103	110	PF00069	0.583
MOD_GSK3_1	111	118	PF00069	0.531
MOD_GSK3_1	262	269	PF00069	0.471
MOD_GSK3_1	370	377	PF00069	0.535
MOD_GSK3_1	412	419	PF00069	0.665
MOD_GSK3_1	42	49	PF00069	0.457
MOD_GSK3_1	422	429	PF00069	0.589
MOD_GSK3_1	476	483	PF00069	0.518
MOD_GSK3_1	519	526	PF00069	0.366
MOD_GSK3_1	528	535	PF00069	0.351
MOD_GSK3_1	67	74	PF00069	0.534
MOD_N-GLC_1	165	170	PF02516	0.372
MOD_N-GLC_1	235	240	PF02516	0.303
MOD_N-GLC_1	83	88	PF02516	0.309
MOD_NEK2_1	145	150	PF00069	0.509
MOD_NEK2_1	235	240	PF00069	0.517
MOD_NEK2_1	266	271	PF00069	0.506
MOD_NEK2_1	345	350	PF00069	0.493
MOD_NEK2_1	537	542	PF00069	0.458
MOD_NEK2_1	83	88	PF00069	0.509
MOD_OFUCOSY	102	107	PF10250	0.428
MOD_PIKK_1	111	117	PF00454	0.534
MOD_PIKK_1	71	77	PF00454	0.534
MOD_PKA_2	103	109	PF00069	0.628
MOD_PKA_2	345	351	PF00069	0.477
MOD_PKA_2	537	543	PF00069	0.474
MOD_Plk_1	165	171	PF00069	0.572
MOD_Plk_1	235	241	PF00069	0.503
MOD_Plk_1	266	272	PF00069	0.526
MOD_Plk_1	519	525	PF00069	0.303
MOD_Plk_1	7	13	PF00069	0.683
MOD_Plk_1	83	89	PF00069	0.509
MOD_Plk_4	145	151	PF00069	0.524
MOD_Plk_4	185	191	PF00069	0.532
MOD_Plk_4	206	212	PF00069	0.471
MOD_Plk_4	345	351	PF00069	0.609
MOD_Plk_4	519	525	PF00069	0.372
MOD_Plk_4	532	538	PF00069	0.346
MOD_Plk_4	577	583	PF00069	0.303
MOD_Plk_4	61	67	PF00069	0.534
MOD_ProDKin_1	116	122	PF00069	0.559
MOD_ProDKin_1	331	337	PF00069	0.627
MOD_ProDKin_1	403	409	PF00069	0.612
MOD_ProDKin_1	416	422	PF00069	0.582
MOD_ProDKin_1	426	432	PF00069	0.609
MOD_ProDKin_1	438	444	PF00069	0.545
MOD_ProDKin_1	480	486	PF00069	0.654
MOD_ProDKin_1	549	555	PF00069	0.380
MOD_SUMO_rev_2	276	281	PF00179	0.398
MOD_SUMO_rev_2	8	17	PF00179	0.661
TRG_ENDOCYTIC_2	208	211	PF00928	0.527
TRG_ENDOCYTIC_2	259	262	PF00928	0.451
TRG_ENDOCYTIC_2	452	455	PF00928	0.567
TRG_ENDOCYTIC_2	458	461	PF00928	0.442
TRG_ER_diArg_1	250	252	PF00400	0.494
TRG_ER_diArg_1	310	313	PF00400	0.673
TRG_ER_diArg_1	318	320	PF00400	0.568
TRG_ER_diArg_1	392	394	PF00400	0.462
TRG_ER_diArg_1	398	401	PF00400	0.548
TRG_ER_diArg_1	512	514	PF00400	0.349
TRG_NES_CRM1_1	182	193	PF08389	0.534
TRG_Pf-PMV_PEXEL_1	588	592	PF00026	0.535

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JVP8	Bodo saltans	40%	100%
A0A1X0NJL3	Trypanosomatidae	56%	100%
A0A3S7WQT0	Leishmania donovani	70%	100%
A4H5T6	Leishmania braziliensis	69%	100%
A4H5T7	Leishmania braziliensis	87%	100%
A4HU29	Leishmania infantum	70%	100%
A4HU30	Leishmania infantum	94%	99%
C9WPN6	Rattus norvegicus	39%	100%
E9AMV9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	70%	100%
F1QGW6	Danio rerio	39%	100%
P41091	Homo sapiens	39%	100%
P81795	Rattus norvegicus	39%	100%
Q2KHU8	Bos taurus	39%	100%
Q2VIR3	Homo sapiens	38%	100%
Q4QHR6	Leishmania major	95%	100%
Q4QHR7	Leishmania major	70%	100%
Q5R797	Pongo abelii	39%	100%
Q5ZMS3	Gallus gallus	38%	100%
Q9Z0N1	Mus musculus	39%	100%
Q9Z0N2	Mus musculus	39%	100%
V5DEC0	Trypanosoma cruzi	45%	100%