Putative cleavage and polyadenylation specificity factor 30 kDa subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cleavage and polyadenylation specificity factor 30 kDa subunit, putative

Species:

Leishmania mexicana

UniProt:

E9AMS3_LEIMU

TriTrypDb:

LmxM.09.0720

Length:

343

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex	4	1
GO:0005849	mRNA cleavage factor complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

E9AMS3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMS3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006379	mRNA cleavage	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	12
GO:0090501	RNA phosphodiester bond hydrolysis	6	12
GO:0098787	mRNA cleavage involved in mRNA processing	8	12
GO:0098789	pre-mRNA cleavage required for polyadenylation	9	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0031426	polycistronic mRNA processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0005488	binding	1	12
GO:0008270	zinc ion binding	6	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0046914	transition metal ion binding	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	122	124	PF00675	0.390
CLV_NRD_NRD_1	250	252	PF00675	0.297
CLV_NRD_NRD_1	298	300	PF00675	0.649
CLV_NRD_NRD_1	301	303	PF00675	0.642
CLV_NRD_NRD_1	336	338	PF00675	0.745
CLV_PCSK_FUR_1	299	303	PF00082	0.746
CLV_PCSK_KEX2_1	122	124	PF00082	0.397
CLV_PCSK_KEX2_1	138	140	PF00082	0.418
CLV_PCSK_KEX2_1	174	176	PF00082	0.559
CLV_PCSK_KEX2_1	250	252	PF00082	0.420
CLV_PCSK_KEX2_1	297	299	PF00082	0.648
CLV_PCSK_KEX2_1	301	303	PF00082	0.640
CLV_PCSK_KEX2_1	335	337	PF00082	0.753
CLV_PCSK_PC1ET2_1	138	140	PF00082	0.543
CLV_PCSK_PC1ET2_1	174	176	PF00082	0.552
CLV_PCSK_PC7_1	297	303	PF00082	0.747
CLV_PCSK_SKI1_1	135	139	PF00082	0.396
CLV_PCSK_SKI1_1	174	178	PF00082	0.565
CLV_PCSK_SKI1_1	192	196	PF00082	0.391
CLV_PCSK_SKI1_1	251	255	PF00082	0.418
DEG_Nend_Nbox_1	1	3	PF02207	0.501
DOC_MAPK_gen_1	174	182	PF00069	0.410
DOC_USP7_MATH_1	111	115	PF00917	0.580
DOC_USP7_MATH_1	214	218	PF00917	0.593
DOC_USP7_MATH_1	285	289	PF00917	0.743
LIG_14-3-3_CanoR_1	139	147	PF00244	0.259
LIG_14-3-3_CanoR_1	48	57	PF00244	0.505
LIG_Actin_WH2_2	54	69	PF00022	0.358
LIG_BIR_III_2	43	47	PF00653	0.539
LIG_BIR_III_4	5	9	PF00653	0.588
LIG_FHA_1	231	237	PF00498	0.722
LIG_FHA_2	259	265	PF00498	0.400
LIG_FHA_2	7	13	PF00498	0.588
LIG_FHA_2	98	104	PF00498	0.448
LIG_LIR_Nem_3	52	57	PF02991	0.523
LIG_PCNA_PIPBox_1	58	67	PF02747	0.368
LIG_REV1ctd_RIR_1	90	99	PF16727	0.539
LIG_SH2_CRK	54	58	PF00017	0.516
LIG_SH2_GRB2like	82	85	PF00017	0.510
LIG_SH2_STAP1	167	171	PF00017	0.498
LIG_SH2_STAP1	82	86	PF00017	0.365
LIG_SH2_STAT3	86	89	PF00017	0.481
LIG_SH2_STAT5	146	149	PF00017	0.462
LIG_SH2_STAT5	78	81	PF00017	0.274
LIG_SH2_STAT5	86	89	PF00017	0.338
LIG_SH3_3	124	130	PF00018	0.382
LIG_SH3_3	149	155	PF00018	0.499
LIG_SH3_3	206	212	PF00018	0.448
LIG_SH3_3	226	232	PF00018	0.344
LIG_SH3_3	279	285	PF00018	0.797
LIG_SH3_3	99	105	PF00018	0.437
LIG_TRAF2_1	162	165	PF00917	0.465
MOD_CK2_1	111	117	PF00069	0.446
MOD_CK2_1	97	103	PF00069	0.465
MOD_Cter_Amidation	333	336	PF01082	0.676
MOD_GlcNHglycan	140	143	PF01048	0.582
MOD_GlcNHglycan	192	195	PF01048	0.517
MOD_GlcNHglycan	287	290	PF01048	0.757
MOD_GlcNHglycan	312	315	PF01048	0.803
MOD_GlcNHglycan	51	54	PF01048	0.469
MOD_GSK3_1	186	193	PF00069	0.557
MOD_GSK3_1	49	56	PF00069	0.395
MOD_LATS_1	136	142	PF00433	0.519
MOD_N-GLC_1	111	116	PF02516	0.494
MOD_N-GLC_1	190	195	PF02516	0.406
MOD_NEK2_1	106	111	PF00069	0.627
MOD_NEK2_1	13	18	PF00069	0.538
MOD_NEK2_1	190	195	PF00069	0.527
MOD_PIKK_1	111	117	PF00454	0.483
MOD_PKA_1	138	144	PF00069	0.516
MOD_PKA_2	138	144	PF00069	0.468
MOD_Plk_1	13	19	PF00069	0.543
MOD_Plk_2-3	258	264	PF00069	0.417
MOD_Plk_4	214	220	PF00069	0.574
MOD_SUMO_rev_2	20	28	PF00179	0.612
TRG_ENDOCYTIC_2	54	57	PF00928	0.520
TRG_ER_diArg_1	121	123	PF00400	0.377
TRG_ER_diArg_1	297	299	PF00400	0.737
TRG_ER_diArg_1	300	302	PF00400	0.738
TRG_ER_diArg_1	335	337	PF00400	0.755
TRG_ER_diArg_1	38	41	PF00400	0.539
TRG_Pf-PMV_PEXEL_1	221	225	PF00026	0.587
TRG_Pf-PMV_PEXEL_1	298	303	PF00026	0.752

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE4	Leptomonas seymouri	86%	100%
A0A0S4KP33	Bodo saltans	49%	99%
A0A1X0NMX4	Trypanosomatidae	58%	100%
A0A3Q8IAT5	Leishmania donovani	98%	100%
A0A3R7KCZ6	Trypanosoma rangeli	60%	100%
A4H5Q3	Leishmania braziliensis	96%	100%
A4HTZ3	Leishmania infantum	98%	100%
D0A9F4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
Q4QHV2	Leishmania major	97%	100%
V5D8L4	Trypanosoma cruzi	60%	100%