tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania mexicana

UniProt:

E9AMR7_LEIMU

TriTrypDb:

LmxM.09.0660

Length:

346

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AMR7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMR7

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006396	RNA processing	6	13
GO:0006399	tRNA metabolic process	7	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008033	tRNA processing	8	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0032259	methylation	2	13
GO:0034470	ncRNA processing	7	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0034660	ncRNA metabolic process	6	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0001510	RNA methylation	4	2
GO:0006400	tRNA modification	6	2
GO:0009451	RNA modification	5	2
GO:0030488	tRNA methylation	5	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0008168	methyltransferase activity	4	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0008173	RNA methyltransferase activity	4	2
GO:0008175	tRNA methyltransferase activity	5	2
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140101	catalytic activity, acting on a tRNA	4	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	25	27	PF00675	0.652
CLV_PCSK_FUR_1	133	137	PF00082	0.423
CLV_PCSK_KEX2_1	135	137	PF00082	0.303
CLV_PCSK_PC1ET2_1	135	137	PF00082	0.299
CLV_PCSK_SKI1_1	108	112	PF00082	0.312
CLV_PCSK_SKI1_1	259	263	PF00082	0.213
DEG_SCF_TRCP1_1	268	274	PF00400	0.350
DEG_SPOP_SBC_1	100	104	PF00917	0.363
DOC_CKS1_1	16	21	PF01111	0.560
DOC_CKS1_1	168	173	PF01111	0.381
DOC_CYCLIN_RxL_1	253	263	PF00134	0.370
DOC_CYCLIN_yCln2_LP_2	295	301	PF00134	0.280
DOC_MAPK_MEF2A_6	236	243	PF00069	0.390
DOC_MAPK_MEF2A_6	288	297	PF00069	0.272
DOC_PP2B_LxvP_1	231	234	PF13499	0.398
DOC_PP2B_LxvP_1	295	298	PF13499	0.272
DOC_USP7_MATH_1	189	193	PF00917	0.387
DOC_USP7_MATH_1	264	268	PF00917	0.335
DOC_USP7_MATH_1	78	82	PF00917	0.398
DOC_USP7_UBL2_3	23	27	PF12436	0.650
DOC_USP7_UBL2_3	52	56	PF12436	0.442
DOC_WW_Pin1_4	126	131	PF00397	0.415
DOC_WW_Pin1_4	15	20	PF00397	0.502
DOC_WW_Pin1_4	167	172	PF00397	0.344
DOC_WW_Pin1_4	74	79	PF00397	0.256
LIG_14-3-3_CanoR_1	236	242	PF00244	0.388
LIG_BIR_II_1	1	5	PF00653	0.680
LIG_BIR_III_2	12	16	PF00653	0.548
LIG_BIR_III_2	3	7	PF00653	0.489
LIG_CtBP_PxDLS_1	154	158	PF00389	0.308
LIG_FHA_1	236	242	PF00498	0.382
LIG_FHA_1	6	12	PF00498	0.734
LIG_FHA_2	167	173	PF00498	0.347
LIG_FHA_2	93	99	PF00498	0.294
LIG_LYPXL_SIV_4	312	320	PF13949	0.381
LIG_PCNA_PIPBox_1	157	166	PF02747	0.398
LIG_PDZ_Class_2	341	346	PF00595	0.425
LIG_SH2_STAT5	196	199	PF00017	0.144
LIG_SH2_STAT5	313	316	PF00017	0.283
LIG_SH2_STAT5	99	102	PF00017	0.295
LIG_SH3_3	165	171	PF00018	0.341
LIG_SH3_3	19	25	PF00018	0.548
LIG_SH3_3	273	279	PF00018	0.256
LIG_SH3_3	8	14	PF00018	0.626
LIG_SH3_4	23	30	PF00018	0.721
LIG_Sin3_3	258	265	PF02671	0.321
LIG_UBA3_1	111	119	PF00899	0.381
LIG_UBA3_1	317	324	PF00899	0.256
LIG_WW_2	14	17	PF00397	0.490
LIG_WW_3	23	27	PF00397	0.703
MOD_CDK_SPK_2	74	79	PF00069	0.256
MOD_CDK_SPxxK_3	126	133	PF00069	0.404
MOD_CK1_1	103	109	PF00069	0.272
MOD_CK1_1	190	196	PF00069	0.441
MOD_CK2_1	166	172	PF00069	0.377
MOD_Cter_Amidation	279	282	PF01082	0.272
MOD_GlcNHglycan	105	108	PF01048	0.263
MOD_GlcNHglycan	193	196	PF01048	0.379
MOD_GlcNHglycan	262	265	PF01048	0.318
MOD_GlcNHglycan	268	271	PF01048	0.356
MOD_GlcNHglycan	273	276	PF01048	0.389
MOD_GlcNHglycan	288	291	PF01048	0.354
MOD_GlcNHglycan	46	49	PF01048	0.624
MOD_GSK3_1	111	118	PF00069	0.278
MOD_GSK3_1	187	194	PF00069	0.361
MOD_GSK3_1	260	267	PF00069	0.297
MOD_GSK3_1	74	81	PF00069	0.386
MOD_GSK3_1	99	106	PF00069	0.315
MOD_N-GLC_2	72	74	PF02516	0.272
MOD_NEK2_1	111	116	PF00069	0.235
MOD_NEK2_1	260	265	PF00069	0.322
MOD_NEK2_1	335	340	PF00069	0.363
MOD_NEK2_2	237	242	PF00069	0.291
MOD_PIKK_1	306	312	PF00454	0.424
MOD_PKA_2	235	241	PF00069	0.357
MOD_PKA_2	271	277	PF00069	0.286
MOD_Plk_1	335	341	PF00069	0.381
MOD_Plk_4	335	341	PF00069	0.166
MOD_ProDKin_1	126	132	PF00069	0.415
MOD_ProDKin_1	15	21	PF00069	0.505
MOD_ProDKin_1	167	173	PF00069	0.344
MOD_ProDKin_1	74	80	PF00069	0.256
MOD_SUMO_for_1	241	244	PF00179	0.305
MOD_SUMO_rev_2	323	332	PF00179	0.350
MOD_SUMO_rev_2	77	87	PF00179	0.291
TRG_DiLeu_BaEn_1	256	261	PF01217	0.308
TRG_DiLeu_BaLyEn_6	330	335	PF01217	0.407
TRG_DiLeu_BaLyEn_6	35	40	PF01217	0.549
TRG_NLS_MonoExtN_4	23	30	PF00514	0.692
TRG_Pf-PMV_PEXEL_1	38	42	PF00026	0.438

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P754	Leptomonas seymouri	65%	100%
A0A0S4JMK0	Bodo saltans	41%	100%
A0A1X0NNC3	Trypanosomatidae	52%	100%
A0A3Q8IBW6	Leishmania donovani	92%	98%
A0A3S5IRY7	Trypanosoma rangeli	46%	100%
A4H5P7	Leishmania braziliensis	83%	100%
A4HTY7	Leishmania infantum	92%	98%
D0A9E3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	97%
P53177	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q4Q4P7	Leishmania major	29%	97%
Q4QHV8	Leishmania major	89%	100%
V5DJH9	Trypanosoma cruzi	48%	89%