EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Repeat of unknown function (DUF1126), putative

Species:

Leishmania mexicana

UniProt:

E9AMI2_LEIMU

TriTrypDb:

LmxM.08.1120

Length:

861

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 30
NetGPI	no	yes: 0, no: 31

Cellular components
Term	Name	Level	Count
GO:0005819	spindle	5	3
GO:0005930	axoneme	2	3
GO:0043226	organelle	2	3
GO:0043228	non-membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043232	intracellular non-membrane-bounded organelle	4	3
GO:0072686	mitotic spindle	6	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

E9AMI2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMI2

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	3
GO:0000281	mitotic cytokinesis	4	3
GO:0000910	cytokinesis	3	3
GO:0001539	cilium or flagellum-dependent cell motility	3	3
GO:0006996	organelle organization	4	3
GO:0007010	cytoskeleton organization	5	3
GO:0007017	microtubule-based process	2	3
GO:0007051	spindle organization	3	3
GO:0007052	mitotic spindle organization	4	3
GO:0009987	cellular process	1	3
GO:0016043	cellular component organization	3	3
GO:0022402	cell cycle process	2	3
GO:0048870	cell motility	2	3
GO:0060285	cilium-dependent cell motility	4	3
GO:0061640	cytoskeleton-dependent cytokinesis	4	3
GO:0071840	cellular component organization or biogenesis	2	3
GO:1902850	microtubule cytoskeleton organization involved in mitosis	4	3
GO:1903047	mitotic cell cycle process	3	3

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	22
GO:0005509	calcium ion binding	5	21
GO:0043167	ion binding	2	21
GO:0043169	cation binding	3	21
GO:0046872	metal ion binding	4	21
GO:0005515	protein binding	2	3
GO:0008092	cytoskeletal protein binding	3	3
GO:0015631	tubulin binding	4	3
GO:0043014	alpha-tubulin binding	5	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	163	167	PF00656	0.534
CLV_C14_Caspase3-7	290	294	PF00656	0.403
CLV_C14_Caspase3-7	453	457	PF00656	0.740
CLV_C14_Caspase3-7	740	744	PF00656	0.520
CLV_MEL_PAP_1	30	36	PF00089	0.559
CLV_NRD_NRD_1	100	102	PF00675	0.607
CLV_NRD_NRD_1	139	141	PF00675	0.274
CLV_NRD_NRD_1	423	425	PF00675	0.722
CLV_NRD_NRD_1	497	499	PF00675	0.266
CLV_NRD_NRD_1	530	532	PF00675	0.293
CLV_NRD_NRD_1	727	729	PF00675	0.490
CLV_NRD_NRD_1	766	768	PF00675	0.543
CLV_NRD_NRD_1	788	790	PF00675	0.396
CLV_PCSK_KEX2_1	172	174	PF00082	0.267
CLV_PCSK_KEX2_1	423	425	PF00082	0.659
CLV_PCSK_KEX2_1	497	499	PF00082	0.254
CLV_PCSK_KEX2_1	529	531	PF00082	0.294
CLV_PCSK_KEX2_1	727	729	PF00082	0.514
CLV_PCSK_KEX2_1	766	768	PF00082	0.546
CLV_PCSK_KEX2_1	788	790	PF00082	0.403
CLV_PCSK_KEX2_1	839	841	PF00082	0.439
CLV_PCSK_PC1ET2_1	172	174	PF00082	0.263
CLV_PCSK_PC1ET2_1	529	531	PF00082	0.295
CLV_PCSK_PC1ET2_1	839	841	PF00082	0.576
CLV_PCSK_SKI1_1	110	114	PF00082	0.382
CLV_PCSK_SKI1_1	220	224	PF00082	0.430
CLV_PCSK_SKI1_1	385	389	PF00082	0.294
CLV_PCSK_SKI1_1	397	401	PF00082	0.487
CLV_PCSK_SKI1_1	424	428	PF00082	0.692
CLV_PCSK_SKI1_1	434	438	PF00082	0.708
CLV_PCSK_SKI1_1	447	451	PF00082	0.767
CLV_PCSK_SKI1_1	483	487	PF00082	0.302
CLV_PCSK_SKI1_1	497	501	PF00082	0.296
CLV_PCSK_SKI1_1	532	536	PF00082	0.320
CLV_PCSK_SKI1_1	543	547	PF00082	0.336
CLV_PCSK_SKI1_1	727	731	PF00082	0.483
CLV_PCSK_SKI1_1	736	740	PF00082	0.416
DEG_APCC_DBOX_1	219	227	PF00400	0.413
DOC_ANK_TNKS_1	291	298	PF00023	0.518
DOC_CKS1_1	473	478	PF01111	0.385
DOC_CKS1_1	781	786	PF01111	0.428
DOC_CYCLIN_RxL_1	431	440	PF00134	0.493
DOC_CYCLIN_yClb5_NLxxxL_5	301	307	PF00134	0.503
DOC_CYCLIN_yCln2_LP_2	29	32	PF00134	0.568
DOC_CYCLIN_yCln2_LP_2	408	414	PF00134	0.492
DOC_CYCLIN_yCln2_LP_2	486	492	PF00134	0.540
DOC_CYCLIN_yCln2_LP_2	546	552	PF00134	0.367
DOC_MAPK_DCC_7	778	787	PF00069	0.387
DOC_MAPK_FxFP_2	810	813	PF00069	0.374
DOC_MAPK_gen_1	140	146	PF00069	0.490
DOC_MAPK_gen_1	529	536	PF00069	0.403
DOC_MAPK_gen_1	644	652	PF00069	0.379
DOC_MAPK_MEF2A_6	301	309	PF00069	0.480
DOC_MAPK_MEF2A_6	370	378	PF00069	0.485
DOC_PP1_RVXF_1	115	122	PF00149	0.434
DOC_PP1_RVXF_1	279	286	PF00149	0.439
DOC_PP2B_LxvP_1	29	32	PF13499	0.546
DOC_PP2B_LxvP_1	408	411	PF13499	0.503
DOC_PP2B_LxvP_1	546	549	PF13499	0.367
DOC_PP4_FxxP_1	125	128	PF00568	0.531
DOC_PP4_FxxP_1	71	74	PF00568	0.703
DOC_PP4_FxxP_1	773	776	PF00568	0.641
DOC_PP4_FxxP_1	810	813	PF00568	0.352
DOC_SPAK_OSR1_1	498	502	PF12202	0.488
DOC_USP7_MATH_1	12	16	PF00917	0.702
DOC_USP7_MATH_1	432	436	PF00917	0.724
DOC_USP7_MATH_1	601	605	PF00917	0.543
DOC_USP7_MATH_1	729	733	PF00917	0.577
DOC_USP7_MATH_1	856	860	PF00917	0.597
DOC_USP7_MATH_1	88	92	PF00917	0.549
DOC_USP7_UBL2_3	535	539	PF12436	0.490
DOC_WW_Pin1_4	472	477	PF00397	0.666
DOC_WW_Pin1_4	780	785	PF00397	0.395
LIG_14-3-3_CanoR_1	110	115	PF00244	0.290
LIG_14-3-3_CanoR_1	204	213	PF00244	0.570
LIG_14-3-3_CanoR_1	319	329	PF00244	0.506
LIG_14-3-3_CanoR_1	403	409	PF00244	0.552
LIG_14-3-3_CanoR_1	423	427	PF00244	0.510
LIG_14-3-3_CanoR_1	498	504	PF00244	0.432
LIG_14-3-3_CanoR_1	67	72	PF00244	0.649
LIG_14-3-3_CanoR_1	709	714	PF00244	0.644
LIG_14-3-3_CanoR_1	754	760	PF00244	0.356
LIG_14-3-3_CanoR_1	8	12	PF00244	0.711
LIG_APCC_ABBA_1	305	310	PF00400	0.490
LIG_BIR_II_1	1	5	PF00653	0.667
LIG_BIR_III_4	456	460	PF00653	0.536
LIG_BIR_III_4	609	613	PF00653	0.588
LIG_BIR_III_4	697	701	PF00653	0.405
LIG_BRCT_BRCA1_1	192	196	PF00533	0.563
LIG_BRCT_BRCA1_1	253	257	PF00533	0.542
LIG_BRCT_BRCA1_1	271	275	PF00533	0.621
LIG_BRCT_BRCA1_1	414	418	PF00533	0.544
LIG_BRCT_BRCA1_1	506	510	PF00533	0.426
LIG_FHA_1	150	156	PF00498	0.459
LIG_FHA_1	377	383	PF00498	0.457
LIG_FHA_1	431	437	PF00498	0.641
LIG_FHA_1	475	481	PF00498	0.463
LIG_FHA_1	703	709	PF00498	0.648
LIG_FHA_1	827	833	PF00498	0.504
LIG_FHA_2	111	117	PF00498	0.348
LIG_FHA_2	448	454	PF00498	0.686
LIG_FHA_2	473	479	PF00498	0.623
LIG_FHA_2	503	509	PF00498	0.440
LIG_FHA_2	557	563	PF00498	0.470
LIG_FHA_2	593	599	PF00498	0.588
LIG_FHA_2	686	692	PF00498	0.361
LIG_FHA_2	772	778	PF00498	0.581
LIG_FHA_2	798	804	PF00498	0.438
LIG_GBD_Chelix_1	620	628	PF00786	0.457
LIG_Integrin_RGD_1	767	769	PF01839	0.402
LIG_LIR_Apic_2	69	74	PF02991	0.478
LIG_LIR_Apic_2	772	776	PF02991	0.559
LIG_LIR_Apic_2	809	813	PF02991	0.443
LIG_LIR_Gen_1	189	199	PF02991	0.480
LIG_LIR_Gen_1	638	645	PF02991	0.418
LIG_LIR_Gen_1	684	693	PF02991	0.436
LIG_LIR_Nem_3	145	149	PF02991	0.568
LIG_LIR_Nem_3	189	194	PF02991	0.424
LIG_LIR_Nem_3	208	213	PF02991	0.472
LIG_LIR_Nem_3	243	248	PF02991	0.447
LIG_LIR_Nem_3	254	260	PF02991	0.475
LIG_LIR_Nem_3	366	372	PF02991	0.526
LIG_LIR_Nem_3	479	485	PF02991	0.569
LIG_LIR_Nem_3	507	513	PF02991	0.485
LIG_LIR_Nem_3	638	642	PF02991	0.387
LIG_LIR_Nem_3	684	690	PF02991	0.464
LIG_LIR_Nem_3	809	815	PF02991	0.455
LIG_MLH1_MIPbox_1	414	418	PF16413	0.544
LIG_MYND_1	27	31	PF01753	0.652
LIG_PCNA_PIPBox_1	804	813	PF02747	0.369
LIG_PCNA_yPIPBox_3	80	90	PF02747	0.372
LIG_Pex14_2	121	125	PF04695	0.428
LIG_Pex14_2	218	222	PF04695	0.511
LIG_REV1ctd_RIR_1	397	407	PF16727	0.337
LIG_SH2_GRB2like	504	507	PF00017	0.591
LIG_SH2_NCK_1	513	517	PF00017	0.487
LIG_SH2_PTP2	191	194	PF00017	0.540
LIG_SH2_SRC	627	630	PF00017	0.493
LIG_SH2_STAP1	395	399	PF00017	0.504
LIG_SH2_STAP1	513	517	PF00017	0.487
LIG_SH2_STAP1	589	593	PF00017	0.359
LIG_SH2_STAP1	830	834	PF00017	0.474
LIG_SH2_STAT5	191	194	PF00017	0.526
LIG_SH2_STAT5	21	24	PF00017	0.624
LIG_SH2_STAT5	330	333	PF00017	0.534
LIG_SH2_STAT5	395	398	PF00017	0.565
LIG_SH2_STAT5	417	420	PF00017	0.590
LIG_SH2_STAT5	504	507	PF00017	0.460
LIG_SH2_STAT5	558	561	PF00017	0.466
LIG_SH2_STAT5	627	630	PF00017	0.425
LIG_SH2_STAT5	686	689	PF00017	0.399
LIG_SH2_STAT5	763	766	PF00017	0.407
LIG_SH2_STAT5	820	823	PF00017	0.522
LIG_SH2_STAT5	842	845	PF00017	0.338
LIG_SH3_3	174	180	PF00018	0.544
LIG_SH3_3	227	233	PF00018	0.382
LIG_SH3_3	442	448	PF00018	0.752
LIG_SH3_3	534	540	PF00018	0.543
LIG_SH3_3	546	552	PF00018	0.538
LIG_SH3_3	647	653	PF00018	0.502
LIG_SH3_3	778	784	PF00018	0.529
LIG_SH3_3	810	816	PF00018	0.420
LIG_SH3_CIN85_PxpxPR_1	538	543	PF14604	0.540
LIG_SUMO_SIM_anti_2	313	318	PF11976	0.439
LIG_SUMO_SIM_anti_2	688	694	PF11976	0.521
LIG_SUMO_SIM_par_1	150	157	PF11976	0.438
LIG_SUMO_SIM_par_1	410	416	PF11976	0.553
LIG_SUMO_SIM_par_1	613	618	PF11976	0.510
LIG_SUMO_SIM_par_1	647	654	PF11976	0.401
LIG_TRAF2_1	187	190	PF00917	0.478
LIG_WRC_WIRS_1	143	148	PF05994	0.416
LIG_WRC_WIRS_1	636	641	PF05994	0.417
LIG_WRC_WIRS_1	770	775	PF05994	0.428
LIG_WW_3	30	34	PF00397	0.565
MOD_CK1_1	322	328	PF00069	0.416
MOD_CK1_1	440	446	PF00069	0.556
MOD_CK1_1	712	718	PF00069	0.570
MOD_CK1_1	814	820	PF00069	0.566
MOD_CK2_1	262	268	PF00069	0.564
MOD_CK2_1	318	324	PF00069	0.311
MOD_CK2_1	447	453	PF00069	0.669
MOD_CK2_1	502	508	PF00069	0.303
MOD_CK2_1	592	598	PF00069	0.521
MOD_CK2_1	648	654	PF00069	0.401
MOD_CK2_1	797	803	PF00069	0.450
MOD_Cter_Amidation	764	767	PF01082	0.569
MOD_GlcNHglycan	162	165	PF01048	0.304
MOD_GlcNHglycan	253	256	PF01048	0.597
MOD_GlcNHglycan	263	267	PF01048	0.542
MOD_GlcNHglycan	338	341	PF01048	0.343
MOD_GlcNHglycan	464	467	PF01048	0.732
MOD_GlcNHglycan	514	517	PF01048	0.310
MOD_GlcNHglycan	603	606	PF01048	0.568
MOD_GlcNHglycan	714	717	PF01048	0.651
MOD_GlcNHglycan	97	100	PF01048	0.443
MOD_GSK3_1	258	265	PF00069	0.584
MOD_GSK3_1	318	325	PF00069	0.325
MOD_GSK3_1	422	429	PF00069	0.595
MOD_GSK3_1	472	479	PF00069	0.626
MOD_GSK3_1	597	604	PF00069	0.571
MOD_GSK3_1	619	626	PF00069	0.445
MOD_GSK3_1	627	634	PF00069	0.376
MOD_GSK3_1	63	70	PF00069	0.676
MOD_GSK3_1	681	688	PF00069	0.410
MOD_GSK3_1	793	800	PF00069	0.512
MOD_GSK3_1	814	821	PF00069	0.349
MOD_LATS_1	629	635	PF00433	0.525
MOD_N-GLC_1	12	17	PF02516	0.605
MOD_N-GLC_1	205	210	PF02516	0.224
MOD_N-GLC_1	67	72	PF02516	0.607
MOD_N-GLC_1	825	830	PF02516	0.524
MOD_NEK2_1	329	334	PF00069	0.305
MOD_NEK2_1	383	388	PF00069	0.224
MOD_NEK2_1	437	442	PF00069	0.571
MOD_NEK2_1	499	504	PF00069	0.256
MOD_NEK2_1	57	62	PF00069	0.634
MOD_NEK2_1	7	12	PF00069	0.651
MOD_NEK2_1	755	760	PF00069	0.348
MOD_NEK2_1	78	83	PF00069	0.484
MOD_NEK2_1	89	94	PF00069	0.456
MOD_NEK2_2	404	409	PF00069	0.347
MOD_NEK2_2	476	481	PF00069	0.364
MOD_PIKK_1	258	264	PF00454	0.499
MOD_PIKK_1	818	824	PF00454	0.621
MOD_PK_1	338	344	PF00069	0.364
MOD_PK_1	704	710	PF00069	0.444
MOD_PKA_1	497	503	PF00069	0.329
MOD_PKA_2	291	297	PF00069	0.364
MOD_PKA_2	318	324	PF00069	0.316
MOD_PKA_2	422	428	PF00069	0.625
MOD_PKA_2	497	503	PF00069	0.319
MOD_PKA_2	57	63	PF00069	0.619
MOD_PKA_2	66	72	PF00069	0.700
MOD_PKA_2	681	687	PF00069	0.563
MOD_PKA_2	7	13	PF00069	0.706
MOD_PKA_2	777	783	PF00069	0.480
MOD_PKA_2	95	101	PF00069	0.639
MOD_Plk_1	149	155	PF00069	0.294
MOD_Plk_1	269	275	PF00069	0.553
MOD_Plk_1	310	316	PF00069	0.294
MOD_Plk_1	346	352	PF00069	0.336
MOD_Plk_1	412	418	PF00069	0.503
MOD_Plk_1	653	659	PF00069	0.515
MOD_Plk_1	67	73	PF00069	0.663
MOD_Plk_4	133	139	PF00069	0.392
MOD_Plk_4	149	155	PF00069	0.309
MOD_Plk_4	413	419	PF00069	0.584
MOD_Plk_4	432	438	PF00069	0.674
MOD_Plk_4	584	590	PF00069	0.540
MOD_Plk_4	635	641	PF00069	0.500
MOD_Plk_4	777	783	PF00069	0.475
MOD_Plk_4	845	851	PF00069	0.621
MOD_ProDKin_1	472	478	PF00069	0.647
MOD_ProDKin_1	780	786	PF00069	0.393
MOD_SUMO_rev_2	561	569	PF00179	0.413
MOD_SUMO_rev_2	715	725	PF00179	0.560
MOD_SUMO_rev_2	732	740	PF00179	0.495
TRG_DiLeu_BaEn_1	803	808	PF01217	0.344
TRG_DiLeu_BaEn_2	303	309	PF01217	0.304
TRG_DiLeu_BaEn_4	189	195	PF01217	0.416
TRG_ENDOCYTIC_2	122	125	PF00928	0.319
TRG_ENDOCYTIC_2	191	194	PF00928	0.427
TRG_ENDOCYTIC_2	286	289	PF00928	0.363
TRG_ENDOCYTIC_2	369	372	PF00928	0.418
TRG_ENDOCYTIC_2	841	844	PF00928	0.425
TRG_ER_diArg_1	180	183	PF00400	0.296
TRG_ER_diArg_1	497	499	PF00400	0.310
TRG_ER_diArg_1	787	789	PF00400	0.374
TRG_NLS_MonoCore_2	528	533	PF00514	0.416
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.417
TRG_Pf-PMV_PEXEL_1	733	737	PF00026	0.430
TRG_Pf-PMV_PEXEL_1	789	793	PF00026	0.368

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5H4	Leptomonas seymouri	70%	100%
A0A0N1HZ98	Leptomonas seymouri	22%	100%
A0A0N1I3M2	Leptomonas seymouri	23%	100%
A0A0N1PBW1	Leptomonas seymouri	24%	100%
A0A0S4IJW8	Bodo saltans	35%	100%
A0A0S4IQM0	Bodo saltans	25%	100%
A0A1X0NEV8	Trypanosomatidae	46%	100%
A0A1X0NLI2	Trypanosomatidae	23%	100%
A0A3Q8IDH4	Leishmania donovani	23%	100%
A0A3Q8IPU3	Leishmania donovani	23%	100%
A0A3R7KM50	Trypanosoma rangeli	47%	100%
A0A3R7L048	Trypanosoma rangeli	24%	100%
A0A3S5H688	Leishmania donovani	95%	99%
A0A422NK00	Trypanosoma rangeli	26%	100%
A4HE78	Leishmania braziliensis	22%	99%
A4HFK9	Leishmania braziliensis	23%	97%
A4HTP5	Leishmania infantum	94%	99%
A4I1J2	Leishmania infantum	23%	100%
C9ZJX4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	100%
C9ZPE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9AD64	Leishmania major	23%	100%
E9AHF6	Leishmania infantum	23%	100%
E9AI34	Leishmania braziliensis	88%	100%
E9AU74	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AXM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9AYY9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
Q4Q9U5	Leishmania major	24%	100%
Q4QI57	Leishmania major	94%	100%
V5ASV2	Trypanosoma cruzi	24%	100%
V5D3X9	Trypanosoma cruzi	23%	100%