EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2453), putative

Species:

Leishmania mexicana

UniProt:

E9AMH1_LEIMU

TriTrypDb:

LmxM.08.0970

Length:

701

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	7
GO:0110165	cellular anatomical entity	1	7
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

E9AMH1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMH1

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	6
GO:0005509	calcium ion binding	5	6
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	309	313	PF00656	0.429
CLV_C14_Caspase3-7	410	414	PF00656	0.412
CLV_C14_Caspase3-7	76	80	PF00656	0.522
CLV_NRD_NRD_1	214	216	PF00675	0.690
CLV_NRD_NRD_1	280	282	PF00675	0.572
CLV_NRD_NRD_1	325	327	PF00675	0.687
CLV_NRD_NRD_1	335	337	PF00675	0.671
CLV_PCSK_FUR_1	248	252	PF00082	0.635
CLV_PCSK_KEX2_1	250	252	PF00082	0.629
CLV_PCSK_KEX2_1	280	282	PF00082	0.572
CLV_PCSK_KEX2_1	325	327	PF00082	0.687
CLV_PCSK_KEX2_1	334	336	PF00082	0.681
CLV_PCSK_PC1ET2_1	250	252	PF00082	0.629
CLV_PCSK_PC1ET2_1	334	336	PF00082	0.768
CLV_PCSK_PC7_1	246	252	PF00082	0.638
CLV_PCSK_SKI1_1	141	145	PF00082	0.727
CLV_PCSK_SKI1_1	229	233	PF00082	0.741
CLV_PCSK_SKI1_1	280	284	PF00082	0.577
CLV_PCSK_SKI1_1	336	340	PF00082	0.785
CLV_PCSK_SKI1_1	6	10	PF00082	0.637
CLV_PCSK_SKI1_1	678	682	PF00082	0.660
DEG_APCC_DBOX_1	279	287	PF00400	0.456
DEG_APCC_DBOX_1	5	13	PF00400	0.433
DEG_SCF_FBW7_1	489	496	PF00400	0.454
DEG_SCF_FBW7_2	66	73	PF00400	0.436
DEG_SCF_SKP2-CKS1_1	327	334	PF00560	0.532
DEG_SIAH_1	56	64	PF03145	0.475
DEG_SPOP_SBC_1	157	161	PF00917	0.496
DEG_SPOP_SBC_1	378	382	PF00917	0.523
DOC_CKS1_1	490	495	PF01111	0.450
DOC_MAPK_DCC_7	585	593	PF00069	0.530
DOC_MAPK_gen_1	401	408	PF00069	0.574
DOC_MAPK_MEF2A_6	585	593	PF00069	0.530
DOC_USP7_MATH_1	109	113	PF00917	0.425
DOC_USP7_MATH_1	135	139	PF00917	0.578
DOC_USP7_MATH_1	151	155	PF00917	0.469
DOC_USP7_MATH_1	156	160	PF00917	0.455
DOC_USP7_MATH_1	164	168	PF00917	0.434
DOC_USP7_MATH_1	187	191	PF00917	0.515
DOC_USP7_MATH_1	193	197	PF00917	0.531
DOC_USP7_MATH_1	202	206	PF00917	0.481
DOC_USP7_MATH_1	23	27	PF00917	0.470
DOC_USP7_MATH_1	338	342	PF00917	0.439
DOC_USP7_MATH_1	345	349	PF00917	0.446
DOC_USP7_MATH_1	353	357	PF00917	0.520
DOC_USP7_MATH_1	378	382	PF00917	0.540
DOC_USP7_MATH_1	448	452	PF00917	0.466
DOC_USP7_MATH_1	468	472	PF00917	0.497
DOC_USP7_MATH_1	501	505	PF00917	0.491
DOC_USP7_MATH_1	560	564	PF00917	0.306
DOC_USP7_MATH_1	64	68	PF00917	0.501
DOC_USP7_MATH_1	648	652	PF00917	0.421
DOC_USP7_UBL2_3	657	661	PF12436	0.433
DOC_WW_Pin1_4	162	167	PF00397	0.510
DOC_WW_Pin1_4	185	190	PF00397	0.514
DOC_WW_Pin1_4	217	222	PF00397	0.482
DOC_WW_Pin1_4	240	245	PF00397	0.489
DOC_WW_Pin1_4	328	333	PF00397	0.535
DOC_WW_Pin1_4	386	391	PF00397	0.492
DOC_WW_Pin1_4	444	449	PF00397	0.408
DOC_WW_Pin1_4	454	459	PF00397	0.493
DOC_WW_Pin1_4	483	488	PF00397	0.589
DOC_WW_Pin1_4	489	494	PF00397	0.496
DOC_WW_Pin1_4	619	624	PF00397	0.299
DOC_WW_Pin1_4	66	71	PF00397	0.486
LIG_14-3-3_CanoR_1	229	238	PF00244	0.526
LIG_14-3-3_CanoR_1	401	408	PF00244	0.518
LIG_14-3-3_CanoR_1	460	464	PF00244	0.494
LIG_14-3-3_CanoR_1	590	594	PF00244	0.569
LIG_14-3-3_CanoR_1	603	611	PF00244	0.473
LIG_14-3-3_CterR_2	699	701	PF00244	0.402
LIG_BIR_III_4	477	481	PF00653	0.536
LIG_BRCT_BRCA1_1	289	293	PF00533	0.466
LIG_BRCT_BRCA1_1	470	474	PF00533	0.445
LIG_BRCT_BRCA1_1	484	488	PF00533	0.385
LIG_FHA_1	536	542	PF00498	0.291
LIG_FHA_1	620	626	PF00498	0.314
LIG_FHA_1	67	73	PF00498	0.527
LIG_FHA_2	307	313	PF00498	0.413
LIG_FHA_2	408	414	PF00498	0.416
LIG_FHA_2	74	80	PF00498	0.560
LIG_GBD_Chelix_1	253	261	PF00786	0.617
LIG_Integrin_RGD_1	683	685	PF01839	0.590
LIG_LIR_Gen_1	296	304	PF02991	0.488
LIG_LIR_Gen_1	516	525	PF02991	0.347
LIG_LIR_Nem_3	290	295	PF02991	0.401
LIG_LIR_Nem_3	296	300	PF02991	0.412
LIG_LIR_Nem_3	485	491	PF02991	0.519
LIG_LIR_Nem_3	516	520	PF02991	0.354
LIG_MAD2	519	527	PF02301	0.342
LIG_MLH1_MIPbox_1	289	293	PF16413	0.466
LIG_MYND_1	265	269	PF01753	0.403
LIG_NRBOX	256	262	PF00104	0.418
LIG_RPA_C_Fungi	467	479	PF08784	0.677
LIG_SH2_NCK_1	297	301	PF00017	0.616
LIG_SH2_NCK_1	583	587	PF00017	0.456
LIG_SH2_NCK_1	601	605	PF00017	0.260
LIG_SH2_SRC	297	300	PF00017	0.620
LIG_SH2_STAP1	297	301	PF00017	0.616
LIG_SH2_STAP1	320	324	PF00017	0.578
LIG_SH2_STAP1	583	587	PF00017	0.393
LIG_SH2_STAT5	292	295	PF00017	0.611
LIG_SH2_STAT5	511	514	PF00017	0.446
LIG_SH2_STAT5	517	520	PF00017	0.425
LIG_SH2_STAT5	583	586	PF00017	0.511
LIG_SH3_3	169	175	PF00018	0.647
LIG_SH3_3	368	374	PF00018	0.624
LIG_SH3_3	484	490	PF00018	0.685
LIG_Sin3_3	121	128	PF02671	0.710
LIG_Sin3_3	362	369	PF02671	0.524
LIG_TRAF2_1	634	637	PF00917	0.535
LIG_TYR_ITIM	295	300	PF00017	0.510
LIG_UBA3_1	418	424	PF00899	0.589
LIG_WW_3	587	591	PF00397	0.453
MOD_CDC14_SPxK_1	243	246	PF00782	0.697
MOD_CDC14_SPxK_1	457	460	PF00782	0.685
MOD_CDK_SPxK_1	240	246	PF00069	0.704
MOD_CDK_SPxK_1	328	334	PF00069	0.684
MOD_CDK_SPxK_1	454	460	PF00069	0.682
MOD_CDK_SPxxK_3	328	335	PF00069	0.684
MOD_CDK_SPxxK_3	386	393	PF00069	0.613
MOD_CK1_1	102	108	PF00069	0.555
MOD_CK1_1	159	165	PF00069	0.736
MOD_CK1_1	167	173	PF00069	0.644
MOD_CK1_1	197	203	PF00069	0.742
MOD_CK1_1	205	211	PF00069	0.665
MOD_CK1_1	220	226	PF00069	0.588
MOD_CK1_1	263	269	PF00069	0.539
MOD_CK1_1	276	282	PF00069	0.409
MOD_CK1_1	314	320	PF00069	0.466
MOD_CK1_1	341	347	PF00069	0.672
MOD_CK1_1	372	378	PF00069	0.748
MOD_CK1_1	386	392	PF00069	0.572
MOD_CK1_1	395	401	PF00069	0.654
MOD_CK1_1	407	413	PF00069	0.568
MOD_CK1_1	425	431	PF00069	0.648
MOD_CK1_1	456	462	PF00069	0.682
MOD_CK1_1	495	501	PF00069	0.638
MOD_CK1_1	602	608	PF00069	0.453
MOD_CK1_1	82	88	PF00069	0.592
MOD_CK2_1	10	16	PF00069	0.455
MOD_CK2_1	157	163	PF00069	0.548
MOD_CK2_1	313	319	PF00069	0.479
MOD_CK2_1	37	43	PF00069	0.612
MOD_GlcNHglycan	104	107	PF01048	0.661
MOD_GlcNHglycan	137	140	PF01048	0.711
MOD_GlcNHglycan	153	156	PF01048	0.800
MOD_GlcNHglycan	166	169	PF01048	0.571
MOD_GlcNHglycan	172	175	PF01048	0.500
MOD_GlcNHglycan	200	203	PF01048	0.704
MOD_GlcNHglycan	204	207	PF01048	0.706
MOD_GlcNHglycan	208	211	PF01048	0.688
MOD_GlcNHglycan	25	28	PF01048	0.553
MOD_GlcNHglycan	275	278	PF01048	0.467
MOD_GlcNHglycan	32	35	PF01048	0.558
MOD_GlcNHglycan	343	346	PF01048	0.796
MOD_GlcNHglycan	353	356	PF01048	0.550
MOD_GlcNHglycan	357	360	PF01048	0.617
MOD_GlcNHglycan	385	388	PF01048	0.732
MOD_GlcNHglycan	395	398	PF01048	0.672
MOD_GlcNHglycan	424	427	PF01048	0.743
MOD_GlcNHglycan	464	468	PF01048	0.758
MOD_GlcNHglycan	49	52	PF01048	0.574
MOD_GlcNHglycan	501	504	PF01048	0.637
MOD_GlcNHglycan	550	553	PF01048	0.335
MOD_GlcNHglycan	568	571	PF01048	0.183
MOD_GlcNHglycan	57	60	PF01048	0.709
MOD_GlcNHglycan	66	69	PF01048	0.662
MOD_GlcNHglycan	82	85	PF01048	0.555
MOD_GlcNHglycan	87	90	PF01048	0.722
MOD_GlcNHglycan	91	95	PF01048	0.774
MOD_GSK3_1	143	150	PF00069	0.695
MOD_GSK3_1	158	165	PF00069	0.648
MOD_GSK3_1	166	173	PF00069	0.806
MOD_GSK3_1	187	194	PF00069	0.559
MOD_GSK3_1	198	205	PF00069	0.573
MOD_GSK3_1	220	227	PF00069	0.725
MOD_GSK3_1	23	30	PF00069	0.607
MOD_GSK3_1	232	239	PF00069	0.557
MOD_GSK3_1	341	348	PF00069	0.542
MOD_GSK3_1	351	358	PF00069	0.483
MOD_GSK3_1	369	376	PF00069	0.754
MOD_GSK3_1	379	386	PF00069	0.655
MOD_GSK3_1	388	395	PF00069	0.528
MOD_GSK3_1	397	404	PF00069	0.741
MOD_GSK3_1	444	451	PF00069	0.717
MOD_GSK3_1	459	466	PF00069	0.658
MOD_GSK3_1	489	496	PF00069	0.641
MOD_GSK3_1	550	557	PF00069	0.469
MOD_GSK3_1	595	602	PF00069	0.389
MOD_GSK3_1	75	82	PF00069	0.650
MOD_GSK3_1	90	97	PF00069	0.564
MOD_GSK3_1	98	105	PF00069	0.621
MOD_N-GLC_1	35	40	PF02516	0.642
MOD_N-GLC_1	468	473	PF02516	0.727
MOD_N-GLC_1	535	540	PF02516	0.335
MOD_N-GLC_1	98	103	PF02516	0.655
MOD_NEK2_1	224	229	PF00069	0.669
MOD_NEK2_1	327	332	PF00069	0.548
MOD_NEK2_1	37	42	PF00069	0.689
MOD_NEK2_1	463	468	PF00069	0.612
MOD_NEK2_1	534	539	PF00069	0.459
MOD_NEK2_1	550	555	PF00069	0.286
MOD_NEK2_1	627	632	PF00069	0.618
MOD_NEK2_2	501	506	PF00069	0.635
MOD_PIKK_1	100	106	PF00454	0.620
MOD_PIKK_1	338	344	PF00454	0.507
MOD_PIKK_1	554	560	PF00454	0.354
MOD_PIKK_1	602	608	PF00454	0.453
MOD_PK_1	311	317	PF00069	0.494
MOD_PKA_2	236	242	PF00069	0.730
MOD_PKA_2	459	465	PF00069	0.618
MOD_PKA_2	581	587	PF00069	0.353
MOD_PKA_2	589	595	PF00069	0.355
MOD_PKA_2	602	608	PF00069	0.308
MOD_Plk_1	311	317	PF00069	0.494
MOD_Plk_1	404	410	PF00069	0.581
MOD_Plk_1	534	540	PF00069	0.340
MOD_Plk_1	662	668	PF00069	0.530
MOD_Plk_4	109	115	PF00069	0.626
MOD_Plk_4	448	454	PF00069	0.499
MOD_Plk_4	513	519	PF00069	0.542
MOD_Plk_4	535	541	PF00069	0.335
MOD_ProDKin_1	162	168	PF00069	0.641
MOD_ProDKin_1	185	191	PF00069	0.648
MOD_ProDKin_1	217	223	PF00069	0.601
MOD_ProDKin_1	240	246	PF00069	0.609
MOD_ProDKin_1	328	334	PF00069	0.684
MOD_ProDKin_1	386	392	PF00069	0.612
MOD_ProDKin_1	444	450	PF00069	0.499
MOD_ProDKin_1	454	460	PF00069	0.623
MOD_ProDKin_1	483	489	PF00069	0.753
MOD_ProDKin_1	619	625	PF00069	0.299
MOD_ProDKin_1	66	72	PF00069	0.607
MOD_SUMO_for_1	18	21	PF00179	0.594
TRG_DiLeu_BaEn_1	278	283	PF01217	0.456
TRG_DiLeu_BaLyEn_6	620	625	PF01217	0.335
TRG_DiLeu_LyEn_5	278	283	PF01217	0.574
TRG_ENDOCYTIC_2	292	295	PF00928	0.493
TRG_ENDOCYTIC_2	297	300	PF00928	0.502
TRG_ENDOCYTIC_2	517	520	PF00928	0.543
TRG_ER_diArg_1	245	248	PF00400	0.559
TRG_ER_diArg_1	280	282	PF00400	0.448
TRG_ER_diArg_1	324	326	PF00400	0.614
TRG_ER_diArg_1	666	669	PF00400	0.537
TRG_NLS_MonoCore_2	116	121	PF00514	0.720
TRG_NLS_MonoExtN_4	115	121	PF00514	0.722
TRG_NLS_MonoExtN_4	332	338	PF00514	0.723
TRG_Pf-PMV_PEXEL_1	10	14	PF00026	0.609

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1G4	Leptomonas seymouri	37%	78%
A0A3S5H678	Leishmania donovani	84%	84%
A4H5E2	Leishmania braziliensis	66%	100%
A4HTN7	Leishmania infantum	84%	84%
Q4QI72	Leishmania major	81%	99%