DNA polymerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA polymerase

Gene product:

mitochondrial DNA polymerase beta-PAK, putative

Species:

Leishmania mexicana

UniProt:

E9AMG4_LEIMU

TriTrypDb:

LmxM.08.0900

Length:

673

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 3
NetGPI	no	yes: 0, no: 3

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	3
GO:0005737	cytoplasm	2	4
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

E9AMG4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMG4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	4
GO:0006259	DNA metabolic process	4	4
GO:0006281	DNA repair	5	4
GO:0006396	RNA processing	6	4
GO:0006725	cellular aromatic compound metabolic process	3	4
GO:0006807	nitrogen compound metabolic process	2	4
GO:0006950	response to stress	2	4
GO:0006974	DNA damage response	4	4
GO:0008152	metabolic process	1	4
GO:0009987	cellular process	1	4
GO:0016070	RNA metabolic process	5	4
GO:0031123	RNA 3'-end processing	7	4
GO:0033554	cellular response to stress	3	4
GO:0034641	cellular nitrogen compound metabolic process	3	4
GO:0043170	macromolecule metabolic process	3	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044260	obsolete cellular macromolecule metabolic process	3	4
GO:0046483	heterocycle metabolic process	3	4
GO:0050896	response to stimulus	1	4
GO:0051716	cellular response to stimulus	2	4
GO:0071076	RNA 3' uridylation	8	4
GO:0071704	organic substance metabolic process	2	4
GO:0090304	nucleic acid metabolic process	4	4
GO:1901360	organic cyclic compound metabolic process	3	4
GO:0006302	double-strand break repair	6	1
GO:0006303	double-strand break repair via nonhomologous end joining	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	4
GO:0003677	DNA binding	4	4
GO:0003824	catalytic activity	1	4
GO:0003887	DNA-directed DNA polymerase activity	5	4
GO:0005488	binding	1	4
GO:0016740	transferase activity	2	4
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	4
GO:0016779	nucleotidyltransferase activity	4	4
GO:0034061	DNA polymerase activity	4	4
GO:0043167	ion binding	2	3
GO:0043169	cation binding	3	3
GO:0046872	metal ion binding	4	3
GO:0050265	RNA uridylyltransferase activity	4	4
GO:0070569	uridylyltransferase activity	5	4
GO:0097159	organic cyclic compound binding	2	4
GO:0140097	catalytic activity, acting on DNA	3	4
GO:0140098	catalytic activity, acting on RNA	3	4
GO:0140640	catalytic activity, acting on a nucleic acid	2	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	317	321	PF00656	0.426
CLV_NRD_NRD_1	372	374	PF00675	0.205
CLV_NRD_NRD_1	389	391	PF00675	0.208
CLV_NRD_NRD_1	491	493	PF00675	0.205
CLV_NRD_NRD_1	537	539	PF00675	0.226
CLV_NRD_NRD_1	541	543	PF00675	0.209
CLV_PCSK_FUR_1	176	180	PF00082	0.351
CLV_PCSK_FUR_1	227	231	PF00082	0.428
CLV_PCSK_FUR_1	489	493	PF00082	0.226
CLV_PCSK_KEX2_1	178	180	PF00082	0.388
CLV_PCSK_KEX2_1	218	220	PF00082	0.555
CLV_PCSK_KEX2_1	229	231	PF00082	0.356
CLV_PCSK_KEX2_1	389	391	PF00082	0.225
CLV_PCSK_KEX2_1	405	407	PF00082	0.334
CLV_PCSK_KEX2_1	491	493	PF00082	0.205
CLV_PCSK_PC1ET2_1	178	180	PF00082	0.388
CLV_PCSK_PC1ET2_1	218	220	PF00082	0.555
CLV_PCSK_PC1ET2_1	229	231	PF00082	0.356
CLV_PCSK_PC1ET2_1	405	407	PF00082	0.343
CLV_PCSK_PC7_1	225	231	PF00082	0.426
CLV_PCSK_SKI1_1	276	280	PF00082	0.230
CLV_PCSK_SKI1_1	421	425	PF00082	0.226
CLV_PCSK_SKI1_1	476	480	PF00082	0.226
CLV_PCSK_SKI1_1	613	617	PF00082	0.216
CLV_PCSK_SKI1_1	620	624	PF00082	0.194
CLV_PCSK_SKI1_1	655	659	PF00082	0.232
DEG_APCC_DBOX_1	567	575	PF00400	0.426
DEG_SCF_TRCP1_1	320	325	PF00400	0.426
DOC_CYCLIN_RxL_1	225	237	PF00134	0.427
DOC_CYCLIN_yClb5_NLxxxL_5	234	243	PF00134	0.422
DOC_CYCLIN_yCln2_LP_2	186	189	PF00134	0.429
DOC_CYCLIN_yCln2_LP_2	423	426	PF00134	0.426
DOC_MAPK_gen_1	225	234	PF00069	0.422
DOC_MAPK_gen_1	246	254	PF00069	0.322
DOC_MAPK_gen_1	487	495	PF00069	0.416
DOC_MAPK_JIP1_4	323	329	PF00069	0.426
DOC_MAPK_MEF2A_6	248	256	PF00069	0.357
DOC_PP2B_LxvP_1	186	189	PF13499	0.429
DOC_PP2B_LxvP_1	192	195	PF13499	0.410
DOC_PP2B_LxvP_1	423	426	PF13499	0.426
DOC_PP4_FxxP_1	537	540	PF00568	0.426
DOC_USP7_MATH_1	110	114	PF00917	0.502
DOC_USP7_MATH_1	117	121	PF00917	0.542
DOC_USP7_MATH_1	228	232	PF00917	0.427
DOC_USP7_MATH_1	3	7	PF00917	0.554
DOC_USP7_MATH_1	331	335	PF00917	0.426
DOC_USP7_MATH_1	555	559	PF00917	0.486
DOC_USP7_MATH_1	598	602	PF00917	0.382
DOC_USP7_MATH_1	95	99	PF00917	0.568
DOC_USP7_UBL2_3	178	182	PF12436	0.435
DOC_USP7_UBL2_3	246	250	PF12436	0.328
DOC_USP7_UBL2_3	319	323	PF12436	0.515
DOC_USP7_UBL2_3	374	378	PF12436	0.405
DOC_USP7_UBL2_3	539	543	PF12436	0.426
DOC_USP7_UBL2_3	620	624	PF12436	0.425
DOC_USP7_UBL2_3	627	631	PF12436	0.398
DOC_WW_Pin1_4	582	587	PF00397	0.368
DOC_WW_Pin1_4	70	75	PF00397	0.688
LIG_14-3-3_CanoR_1	227	233	PF00244	0.421
LIG_BIR_II_1	1	5	PF00653	0.560
LIG_BIR_III_3	1	5	PF00653	0.560
LIG_BIR_III_4	606	610	PF00653	0.426
LIG_BRCT_BRCA1_1	484	488	PF00533	0.384
LIG_BRCT_BRCA1_1	499	503	PF00533	0.355
LIG_BRCT_BRCA1_1	512	516	PF00533	0.369
LIG_FHA_1	255	261	PF00498	0.378
LIG_FHA_1	463	469	PF00498	0.426
LIG_FHA_1	66	72	PF00498	0.503
LIG_FHA_2	286	292	PF00498	0.517
LIG_FHA_2	628	634	PF00498	0.426
LIG_FHA_2	651	657	PF00498	0.426
LIG_Integrin_RGD_1	179	181	PF01839	0.438
LIG_LIR_Apic_2	664	668	PF02991	0.426
LIG_LIR_Gen_1	181	192	PF02991	0.414
LIG_LIR_Gen_1	365	375	PF02991	0.450
LIG_LIR_Gen_1	379	388	PF02991	0.426
LIG_LIR_Gen_1	513	524	PF02991	0.405
LIG_LIR_Gen_1	556	567	PF02991	0.449
LIG_LIR_Gen_1	656	666	PF02991	0.405
LIG_LIR_LC3C_4	325	329	PF02991	0.426
LIG_LIR_LC3C_4	379	384	PF02991	0.426
LIG_LIR_Nem_3	137	143	PF02991	0.477
LIG_LIR_Nem_3	181	187	PF02991	0.409
LIG_LIR_Nem_3	291	296	PF02991	0.426
LIG_LIR_Nem_3	365	370	PF02991	0.450
LIG_LIR_Nem_3	379	383	PF02991	0.426
LIG_LIR_Nem_3	452	457	PF02991	0.405
LIG_LIR_Nem_3	513	519	PF02991	0.405
LIG_LIR_Nem_3	533	537	PF02991	0.343
LIG_LIR_Nem_3	616	622	PF02991	0.398
LIG_LIR_Nem_3	656	661	PF02991	0.405
LIG_MYND_1	189	193	PF01753	0.455
LIG_MYND_1	422	426	PF01753	0.426
LIG_RPA_C_Insects	609	624	PF08784	0.221
LIG_SH2_CRK	548	552	PF00017	0.259
LIG_SH2_NCK_1	548	552	PF00017	0.201
LIG_SH2_PTP2	665	668	PF00017	0.230
LIG_SH2_SRC	442	445	PF00017	0.230
LIG_SH2_SRC	497	500	PF00017	0.259
LIG_SH2_SRC	665	668	PF00017	0.230
LIG_SH2_STAP1	307	311	PF00017	0.259
LIG_SH2_STAP1	380	384	PF00017	0.230
LIG_SH2_STAT3	388	391	PF00017	0.259
LIG_SH2_STAT5	292	295	PF00017	0.259
LIG_SH2_STAT5	375	378	PF00017	0.266
LIG_SH2_STAT5	442	445	PF00017	0.230
LIG_SH2_STAT5	497	500	PF00017	0.302
LIG_SH2_STAT5	509	512	PF00017	0.184
LIG_SH2_STAT5	665	668	PF00017	0.230
LIG_SH3_3	16	22	PF00018	0.525
LIG_SH3_3	40	46	PF00018	0.518
LIG_SH3_3	419	425	PF00018	0.259
LIG_SH3_3	7	13	PF00018	0.610
LIG_SH3_4	539	546	PF00018	0.259
LIG_SUMO_SIM_anti_2	397	403	PF11976	0.230
LIG_TRAF2_1	357	360	PF00917	0.259
LIG_TRAF2_1	630	633	PF00917	0.259
LIG_TRAF2_1	82	85	PF00917	0.492
LIG_TRFH_1	184	188	PF08558	0.433
LIG_TRFH_1	53	57	PF08558	0.569
LIG_UBA3_1	239	246	PF00899	0.405
MOD_CK1_1	102	108	PF00069	0.573
MOD_CK1_1	120	126	PF00069	0.488
MOD_CK1_1	134	140	PF00069	0.454
MOD_CK1_1	415	421	PF00069	0.215
MOD_CK1_1	594	600	PF00069	0.284
MOD_CK1_1	601	607	PF00069	0.407
MOD_CK2_1	404	410	PF00069	0.327
MOD_CK2_1	41	47	PF00069	0.453
MOD_CK2_1	582	588	PF00069	0.259
MOD_CK2_1	627	633	PF00069	0.259
MOD_CK2_1	650	656	PF00069	0.259
MOD_GlcNHglycan	101	104	PF01048	0.522
MOD_GlcNHglycan	108	111	PF01048	0.498
MOD_GlcNHglycan	112	115	PF01048	0.475
MOD_GlcNHglycan	119	122	PF01048	0.490
MOD_GlcNHglycan	123	126	PF01048	0.490
MOD_GlcNHglycan	158	161	PF01048	0.368
MOD_GlcNHglycan	320	323	PF01048	0.299
MOD_GlcNHglycan	393	396	PF01048	0.233
MOD_GlcNHglycan	418	421	PF01048	0.325
MOD_GlcNHglycan	570	574	PF01048	0.302
MOD_GlcNHglycan	582	585	PF01048	0.216
MOD_GlcNHglycan	593	596	PF01048	0.201
MOD_GlcNHglycan	600	603	PF01048	0.160
MOD_GlcNHglycan	74	77	PF01048	0.561
MOD_GlcNHglycan	93	96	PF01048	0.462
MOD_GSK3_1	102	109	PF00069	0.532
MOD_GSK3_1	116	123	PF00069	0.536
MOD_GSK3_1	152	159	PF00069	0.358
MOD_GSK3_1	166	173	PF00069	0.295
MOD_GSK3_1	228	235	PF00069	0.419
MOD_GSK3_1	314	321	PF00069	0.313
MOD_GSK3_1	400	407	PF00069	0.343
MOD_GSK3_1	412	419	PF00069	0.190
MOD_GSK3_1	462	469	PF00069	0.259
MOD_GSK3_1	594	601	PF00069	0.259
MOD_GSK3_1	609	616	PF00069	0.193
MOD_GSK3_1	66	73	PF00069	0.701
MOD_GSK3_1	91	98	PF00069	0.553
MOD_LATS_1	404	410	PF00433	0.221
MOD_N-GLC_1	482	487	PF02516	0.182
MOD_NEK2_1	314	319	PF00069	0.300
MOD_NEK2_1	508	513	PF00069	0.230
MOD_NEK2_1	569	574	PF00069	0.259
MOD_NEK2_1	91	96	PF00069	0.573
MOD_NEK2_2	555	560	PF00069	0.259
MOD_NEK2_2	67	72	PF00069	0.499
MOD_PIKK_1	152	158	PF00454	0.352
MOD_PIKK_1	164	170	PF00454	0.320
MOD_PIKK_1	276	282	PF00454	0.230
MOD_PIKK_1	80	86	PF00454	0.727
MOD_PKA_1	405	411	PF00069	0.182
MOD_PKA_1	538	544	PF00069	0.259
MOD_PKA_1	613	619	PF00069	0.259
MOD_PKA_2	405	411	PF00069	0.218
MOD_PKA_2	80	86	PF00069	0.575
MOD_Plk_1	276	282	PF00069	0.230
MOD_Plk_1	443	449	PF00069	0.230
MOD_Plk_1	51	57	PF00069	0.569
MOD_Plk_1	555	561	PF00069	0.259
MOD_Plk_1	569	575	PF00069	0.143
MOD_Plk_4	134	140	PF00069	0.538
MOD_Plk_4	546	552	PF00069	0.201
MOD_ProDKin_1	582	588	PF00069	0.177
MOD_ProDKin_1	70	76	PF00069	0.689
MOD_SUMO_for_1	574	577	PF00179	0.259
MOD_SUMO_rev_2	261	271	PF00179	0.372
TRG_DiLeu_BaEn_1	87	92	PF01217	0.492
TRG_DiLeu_BaLyEn_6	419	424	PF01217	0.259
TRG_ENDOCYTIC_2	349	352	PF00928	0.230
TRG_ENDOCYTIC_2	380	383	PF00928	0.473
TRG_ENDOCYTIC_2	534	537	PF00928	0.230
TRG_ENDOCYTIC_2	548	551	PF00928	0.230
TRG_ER_diArg_1	370	373	PF00400	0.230
TRG_ER_diArg_1	388	390	PF00400	0.230
TRG_ER_diArg_1	488	491	PF00400	0.241
TRG_ER_diArg_1	646	649	PF00400	0.233
TRG_NLS_Bipartite_1	613	627	PF00514	0.259
TRG_NLS_MonoExtC_3	619	624	PF00514	0.253
TRG_NLS_MonoExtN_4	199	204	PF00514	0.455
TRG_NLS_MonoExtN_4	620	627	PF00514	0.257
TRG_Pf-PMV_PEXEL_1	273	277	PF00026	0.343
TRG_Pf-PMV_PEXEL_1	655	659	PF00026	0.259

Homologs


Protein	Taxonomy	Sequence identity	Coverage
C9ZPC9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	83%
Q4QI79	Leishmania major	92%	100%
Q67VC8	Oryza sativa subsp. japonica	23%	100%