Translation initiation factor-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor-like protein

Species:

Leishmania mexicana

UniProt:

E9AMD5_LEIMU

TriTrypDb:

LmxM.08.0550

Length:

344

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005850	eukaryotic translation initiation factor 2 complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AMD5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMD5

Function

Biological processes
Term	Name	Level	Count
GO:0001731	formation of translation preinitiation complex	7	1
GO:0001732	formation of cytoplasmic translation initiation complex	7	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0022618	ribonucleoprotein complex assembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0065003	protein-containing complex assembly	5	1
GO:0071826	ribonucleoprotein complex subunit organization	5	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003723	RNA binding	4	1
GO:0003729	mRNA binding	5	1
GO:0005515	protein binding	2	1
GO:0031369	translation initiation factor binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	77	81	PF00656	0.842
CLV_NRD_NRD_1	10	12	PF00675	0.710
CLV_NRD_NRD_1	270	272	PF00675	0.487
CLV_NRD_NRD_1	305	307	PF00675	0.379
CLV_PCSK_FUR_1	7	11	PF00082	0.718
CLV_PCSK_KEX2_1	124	126	PF00082	0.530
CLV_PCSK_KEX2_1	305	307	PF00082	0.379
CLV_PCSK_KEX2_1	334	336	PF00082	0.502
CLV_PCSK_KEX2_1	9	11	PF00082	0.686
CLV_PCSK_PC1ET2_1	124	126	PF00082	0.533
CLV_PCSK_PC1ET2_1	334	336	PF00082	0.502
CLV_PCSK_SKI1_1	318	322	PF00082	0.515
DEG_SPOP_SBC_1	160	164	PF00917	0.588
DOC_MAPK_gen_1	334	344	PF00069	0.532
DOC_MAPK_MEF2A_6	209	216	PF00069	0.450
DOC_PP2B_LxvP_1	212	215	PF13499	0.421
DOC_PP4_FxxP_1	237	240	PF00568	0.342
DOC_USP7_MATH_1	161	165	PF00917	0.666
DOC_USP7_MATH_1	171	175	PF00917	0.683
DOC_USP7_MATH_1	50	54	PF00917	0.777
DOC_USP7_MATH_1	96	100	PF00917	0.672
DOC_WW_Pin1_4	89	94	PF00397	0.657
LIG_14-3-3_CanoR_1	185	190	PF00244	0.480
LIG_14-3-3_CanoR_1	271	280	PF00244	0.482
LIG_APCC_ABBA_1	57	62	PF00400	0.741
LIG_FHA_1	26	32	PF00498	0.825
LIG_FHA_1	315	321	PF00498	0.432
LIG_FHA_2	272	278	PF00498	0.342
LIG_FHA_2	28	34	PF00498	0.872
LIG_FHA_2	82	88	PF00498	0.662
LIG_LIR_Apic_2	235	240	PF02991	0.421
LIG_LIR_Gen_1	110	119	PF02991	0.556
LIG_LIR_Gen_1	155	161	PF02991	0.643
LIG_LIR_Gen_1	274	284	PF02991	0.385
LIG_LIR_Nem_3	110	114	PF02991	0.625
LIG_LIR_Nem_3	115	119	PF02991	0.544
LIG_LIR_Nem_3	155	160	PF02991	0.609
LIG_LIR_Nem_3	191	197	PF02991	0.562
LIG_LIR_Nem_3	274	279	PF02991	0.367
LIG_MYND_3	214	218	PF01753	0.469
LIG_PCNA_yPIPBox_3	185	194	PF02747	0.600
LIG_SH2_CRK	111	115	PF00017	0.618
LIG_SH2_CRK	116	120	PF00017	0.524
LIG_SH2_STAP1	116	120	PF00017	0.571
LIG_SH2_STAT5	283	286	PF00017	0.327
LIG_SH2_STAT5	287	290	PF00017	0.327
LIG_SH3_3	49	55	PF00018	0.748
LIG_SH3_3	65	71	PF00018	0.683
LIG_SH3_3	87	93	PF00018	0.643
LIG_TRAF2_1	130	133	PF00917	0.554
LIG_TRAF2_1	175	178	PF00917	0.638
LIG_TRAF2_1	30	33	PF00917	0.750
LIG_TYR_ITIM	109	114	PF00017	0.611
LIG_UBA3_1	119	124	PF00899	0.552
LIG_UBA3_1	216	224	PF00899	0.469
LIG_UBA3_1	280	289	PF00899	0.385
MOD_CK1_1	15	21	PF00069	0.875
MOD_CK1_1	204	210	PF00069	0.329
MOD_CK1_1	53	59	PF00069	0.826
MOD_CK1_1	72	78	PF00069	0.732
MOD_CK1_1	81	87	PF00069	0.720
MOD_CK2_1	15	21	PF00069	0.762
MOD_CK2_1	171	177	PF00069	0.701
MOD_CK2_1	27	33	PF00069	0.743
MOD_CK2_1	81	87	PF00069	0.697
MOD_Cter_Amidation	220	223	PF01082	0.362
MOD_Cter_Amidation	303	306	PF01082	0.342
MOD_GlcNHglycan	174	177	PF01048	0.600
MOD_GlcNHglycan	202	206	PF01048	0.568
MOD_GlcNHglycan	328	331	PF01048	0.527
MOD_GlcNHglycan	52	55	PF01048	0.783
MOD_GlcNHglycan	71	74	PF01048	0.713
MOD_GSK3_1	12	19	PF00069	0.857
MOD_GSK3_1	159	166	PF00069	0.525
MOD_GSK3_1	27	34	PF00069	0.572
MOD_GSK3_1	312	319	PF00069	0.344
MOD_GSK3_1	322	329	PF00069	0.556
MOD_GSK3_1	78	85	PF00069	0.771
MOD_GSK3_1	96	103	PF00069	0.691
MOD_N-GLC_1	171	176	PF02516	0.686
MOD_N-GLC_2	291	293	PF02516	0.342
MOD_N-GLC_2	312	314	PF02516	0.327
MOD_NEK2_2	55	60	PF00069	0.670
MOD_PIKK_1	63	69	PF00454	0.678
MOD_PK_1	250	256	PF00069	0.342
MOD_PKA_1	124	130	PF00069	0.478
MOD_PKA_1	222	228	PF00069	0.469
MOD_PKA_1	271	277	PF00069	0.469
MOD_PKA_2	124	130	PF00069	0.565
MOD_Plk_1	142	148	PF00069	0.629
MOD_Plk_1	250	256	PF00069	0.341
MOD_Plk_1	296	302	PF00069	0.327
MOD_Plk_2-3	17	23	PF00069	0.773
MOD_Plk_4	185	191	PF00069	0.489
MOD_Plk_4	222	228	PF00069	0.469
MOD_ProDKin_1	89	95	PF00069	0.658
MOD_SUMO_for_1	266	269	PF00179	0.342
MOD_SUMO_rev_2	242	247	PF00179	0.469
TRG_ENDOCYTIC_2	111	114	PF00928	0.584
TRG_ENDOCYTIC_2	116	119	PF00928	0.512
TRG_ENDOCYTIC_2	186	189	PF00928	0.452
TRG_ER_diArg_1	305	307	PF00400	0.378
TRG_ER_diArg_1	7	10	PF00400	0.714
TRG_NLS_MonoExtN_4	219	226	PF00514	0.342
TRG_Pf-PMV_PEXEL_1	282	286	PF00026	0.342

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3Y9	Leptomonas seymouri	71%	98%
A0A0S4KLD6	Bodo saltans	38%	100%
A0A1X0NPQ0	Trypanosomatidae	49%	91%
A0A3R7JYX9	Trypanosoma rangeli	52%	100%
A0A3S5H658	Leishmania donovani	91%	100%
A4H5C4	Leishmania braziliensis	83%	99%
A4HTK4	Leishmania infantum	91%	100%
C9ZPG8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
Q4QIB4	Leishmania major	95%	100%
V5BGW0	Trypanosoma cruzi	53%	100%