Ubiquitin-activating enzyme-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-activating enzyme-like protein

Species:

Leishmania mexicana

UniProt:

E9AMA1_LEIMU

TriTrypDb:

LmxM.08.0220

Length:

1053

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031510	SUMO activating enzyme complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1

Expansion

Sequence features

E9AMA1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AMA1

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008152	metabolic process	1	8
GO:0016925	protein sumoylation	7	8
GO:0018193	peptidyl-amino acid modification	5	8
GO:0018205	peptidyl-lysine modification	6	8
GO:0019538	protein metabolic process	3	8
GO:0032446	protein modification by small protein conjugation	6	8
GO:0036211	protein modification process	4	8
GO:0043170	macromolecule metabolic process	3	8
GO:0043412	macromolecule modification	4	8
GO:0044238	primary metabolic process	2	8
GO:0070647	protein modification by small protein conjugation or removal	5	8
GO:0071704	organic substance metabolic process	2	8
GO:1901564	organonitrogen compound metabolic process	3	8

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	8
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0005524	ATP binding	5	8
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	8
GO:0016874	ligase activity	2	8
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	8
GO:0017076	purine nucleotide binding	4	8
GO:0019948	SUMO activating enzyme activity	3	8
GO:0030554	adenyl nucleotide binding	5	8
GO:0032553	ribonucleotide binding	3	8
GO:0032555	purine ribonucleotide binding	4	8
GO:0032559	adenyl ribonucleotide binding	5	8
GO:0035639	purine ribonucleoside triphosphate binding	4	8
GO:0036094	small molecule binding	2	8
GO:0043167	ion binding	2	8
GO:0043168	anion binding	3	8
GO:0097159	organic cyclic compound binding	2	8
GO:0097367	carbohydrate derivative binding	2	8
GO:0140096	catalytic activity, acting on a protein	2	8
GO:0140657	ATP-dependent activity	1	8
GO:1901265	nucleoside phosphate binding	3	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	182	186	PF00656	0.608
CLV_C14_Caspase3-7	188	192	PF00656	0.479
CLV_C14_Caspase3-7	308	312	PF00656	0.279
CLV_C14_Caspase3-7	390	394	PF00656	0.346
CLV_C14_Caspase3-7	479	483	PF00656	0.346
CLV_NRD_NRD_1	335	337	PF00675	0.346
CLV_NRD_NRD_1	356	358	PF00675	0.283
CLV_NRD_NRD_1	535	537	PF00675	0.338
CLV_NRD_NRD_1	600	602	PF00675	0.346
CLV_NRD_NRD_1	654	656	PF00675	0.346
CLV_NRD_NRD_1	759	761	PF00675	0.493
CLV_NRD_NRD_1	768	770	PF00675	0.642
CLV_NRD_NRD_1	77	79	PF00675	0.601
CLV_PCSK_FUR_1	766	770	PF00082	0.653
CLV_PCSK_KEX2_1	335	337	PF00082	0.346
CLV_PCSK_KEX2_1	356	358	PF00082	0.283
CLV_PCSK_KEX2_1	535	537	PF00082	0.431
CLV_PCSK_KEX2_1	600	602	PF00082	0.346
CLV_PCSK_KEX2_1	766	768	PF00082	0.626
CLV_PCSK_KEX2_1	77	79	PF00082	0.601
CLV_PCSK_SKI1_1	238	242	PF00082	0.585
CLV_PCSK_SKI1_1	276	280	PF00082	0.313
CLV_PCSK_SKI1_1	317	321	PF00082	0.279
CLV_PCSK_SKI1_1	336	340	PF00082	0.279
CLV_PCSK_SKI1_1	573	577	PF00082	0.373
CLV_PCSK_SKI1_1	880	884	PF00082	0.249
DEG_APCC_DBOX_1	316	324	PF00400	0.373
DEG_SCF_FBW7_1	1019	1025	PF00400	0.516
DEG_SPOP_SBC_1	143	147	PF00917	0.747
DEG_SPOP_SBC_1	723	727	PF00917	0.656
DOC_CKS1_1	1019	1024	PF01111	0.517
DOC_CYCLIN_RxL_1	289	300	PF00134	0.313
DOC_CYCLIN_RxL_1	570	578	PF00134	0.346
DOC_MAPK_gen_1	766	772	PF00069	0.728
DOC_MAPK_JIP1_4	648	654	PF00069	0.346
DOC_MAPK_MEF2A_6	777	785	PF00069	0.642
DOC_PP1_RVXF_1	571	577	PF00149	0.346
DOC_PP2B_LxvP_1	207	210	PF13499	0.564
DOC_PP2B_LxvP_1	898	901	PF13499	0.492
DOC_PP2B_LxvP_1	928	931	PF13499	0.492
DOC_USP7_MATH_1	115	119	PF00917	0.666
DOC_USP7_MATH_1	143	147	PF00917	0.747
DOC_USP7_MATH_1	162	166	PF00917	0.443
DOC_USP7_MATH_1	176	180	PF00917	0.579
DOC_USP7_MATH_1	20	24	PF00917	0.635
DOC_USP7_MATH_1	256	260	PF00917	0.556
DOC_USP7_MATH_1	529	533	PF00917	0.306
DOC_USP7_MATH_1	539	543	PF00917	0.284
DOC_USP7_MATH_1	609	613	PF00917	0.393
DOC_USP7_MATH_1	660	664	PF00917	0.401
DOC_USP7_MATH_1	679	683	PF00917	0.177
DOC_USP7_MATH_1	724	728	PF00917	0.685
DOC_USP7_MATH_1	795	799	PF00917	0.641
DOC_USP7_MATH_1	838	842	PF00917	0.477
DOC_USP7_MATH_1	901	905	PF00917	0.503
DOC_USP7_MATH_1	92	96	PF00917	0.548
DOC_USP7_MATH_1	974	978	PF00917	0.522
DOC_WW_Pin1_4	1018	1023	PF00397	0.522
DOC_WW_Pin1_4	126	131	PF00397	0.604
DOC_WW_Pin1_4	238	243	PF00397	0.656
DOC_WW_Pin1_4	251	256	PF00397	0.731
DOC_WW_Pin1_4	259	264	PF00397	0.586
DOC_WW_Pin1_4	358	363	PF00397	0.460
DOC_WW_Pin1_4	616	621	PF00397	0.417
DOC_WW_Pin1_4	748	753	PF00397	0.616
DOC_WW_Pin1_4	770	775	PF00397	0.740
DOC_WW_Pin1_4	777	782	PF00397	0.677
DOC_WW_Pin1_4	95	100	PF00397	0.564
DOC_WW_Pin1_4	972	977	PF00397	0.680
LIG_14-3-3_CanoR_1	455	460	PF00244	0.305
LIG_14-3-3_CanoR_1	528	538	PF00244	0.186
LIG_14-3-3_CanoR_1	560	569	PF00244	0.431
LIG_14-3-3_CanoR_1	717	723	PF00244	0.346
LIG_14-3-3_CanoR_1	777	781	PF00244	0.761
LIG_14-3-3_CanoR_1	880	885	PF00244	0.507
LIG_14-3-3_CanoR_1	942	948	PF00244	0.517
LIG_Actin_WH2_2	701	719	PF00022	0.346
LIG_BIR_II_1	1	5	PF00653	0.585
LIG_BRCT_BRCA1_1	154	158	PF00533	0.543
LIG_BRCT_BRCA1_1	253	257	PF00533	0.491
LIG_Clathr_ClatBox_1	304	308	PF01394	0.373
LIG_FHA_1	1023	1029	PF00498	0.703
LIG_FHA_1	1034	1040	PF00498	0.681
LIG_FHA_1	192	198	PF00498	0.481
LIG_FHA_1	230	236	PF00498	0.675
LIG_FHA_1	44	50	PF00498	0.535
LIG_FHA_1	444	450	PF00498	0.320
LIG_FHA_1	455	461	PF00498	0.279
LIG_FHA_1	874	880	PF00498	0.320
LIG_FHA_2	180	186	PF00498	0.636
LIG_FHA_2	576	582	PF00498	0.346
LIG_FHA_2	961	967	PF00498	0.524
LIG_GBD_Chelix_1	567	575	PF00786	0.373
LIG_GBD_Chelix_1	708	716	PF00786	0.373
LIG_LIR_Gen_1	153	163	PF02991	0.540
LIG_LIR_Gen_1	297	307	PF02991	0.373
LIG_LIR_Gen_1	468	474	PF02991	0.279
LIG_LIR_Gen_1	913	924	PF02991	0.472
LIG_LIR_Gen_1	955	962	PF02991	0.511
LIG_LIR_Nem_3	121	126	PF02991	0.575
LIG_LIR_Nem_3	153	159	PF02991	0.542
LIG_LIR_Nem_3	297	302	PF02991	0.373
LIG_LIR_Nem_3	379	385	PF02991	0.313
LIG_LIR_Nem_3	468	473	PF02991	0.279
LIG_LIR_Nem_3	913	919	PF02991	0.446
LIG_LIR_Nem_3	955	961	PF02991	0.469
LIG_MAD2	196	204	PF02301	0.487
LIG_NRBOX	570	576	PF00104	0.346
LIG_NRBOX	711	717	PF00104	0.373
LIG_PCNA_yPIPBox_3	425	438	PF02747	0.332
LIG_Pex14_2	381	385	PF04695	0.285
LIG_PTAP_UEV_1	244	249	PF05743	0.503
LIG_SH2_NCK_1	377	381	PF00017	0.373
LIG_SH2_SRC	377	380	PF00017	0.373
LIG_SH2_STAP1	377	381	PF00017	0.373
LIG_SH2_STAP1	465	469	PF00017	0.279
LIG_SH2_STAP1	470	474	PF00017	0.279
LIG_SH2_STAP1	758	762	PF00017	0.549
LIG_SH2_STAP1	916	920	PF00017	0.511
LIG_SH2_STAP1	945	949	PF00017	0.492
LIG_SH2_STAT3	420	423	PF00017	0.279
LIG_SH2_STAT5	526	529	PF00017	0.391
LIG_SH2_STAT5	848	851	PF00017	0.481
LIG_SH2_STAT5	945	948	PF00017	0.492
LIG_SH3_1	768	774	PF00018	0.661
LIG_SH3_2	855	860	PF14604	0.493
LIG_SH3_2	932	937	PF14604	0.377
LIG_SH3_3	14	20	PF00018	0.582
LIG_SH3_3	239	245	PF00018	0.695
LIG_SH3_3	436	442	PF00018	0.393
LIG_SH3_3	588	594	PF00018	0.299
LIG_SH3_3	728	734	PF00018	0.557
LIG_SH3_3	739	745	PF00018	0.639
LIG_SH3_3	768	774	PF00018	0.657
LIG_SH3_3	852	858	PF00018	0.432
LIG_SH3_3	929	935	PF00018	0.377
LIG_SUMO_SIM_anti_2	711	717	PF11976	0.373
LIG_SUMO_SIM_anti_2	841	846	PF11976	0.622
LIG_SUMO_SIM_par_1	1035	1042	PF11976	0.630
LIG_SUMO_SIM_par_1	292	297	PF11976	0.341
LIG_SUMO_SIM_par_1	303	309	PF11976	0.274
LIG_SUMO_SIM_par_1	581	589	PF11976	0.313
LIG_SUMO_SIM_par_1	626	633	PF11976	0.325
LIG_SUMO_SIM_par_1	875	881	PF11976	0.492
LIG_SxIP_EBH_1	687	700	PF03271	0.346
LIG_TRAF2_1	1010	1013	PF00917	0.496
LIG_TRAF2_1	991	994	PF00917	0.494
LIG_UBA3_1	571	579	PF00899	0.373
LIG_UBA3_1	583	587	PF00899	0.279
LIG_Vh1_VBS_1	183	201	PF01044	0.437
LIG_WW_3	824	828	PF00397	0.503
MOD_CDK_SPK_2	748	753	PF00069	0.616
MOD_CDK_SPxxK_3	251	258	PF00069	0.656
MOD_CDK_SPxxK_3	770	777	PF00069	0.565
MOD_CK1_1	118	124	PF00069	0.501
MOD_CK1_1	152	158	PF00069	0.627
MOD_CK1_1	165	171	PF00069	0.516
MOD_CK1_1	179	185	PF00069	0.710
MOD_CK1_1	259	265	PF00069	0.565
MOD_CK1_1	297	303	PF00069	0.373
MOD_CK1_1	476	482	PF00069	0.346
MOD_CK1_1	487	493	PF00069	0.242
MOD_CK1_1	502	508	PF00069	0.250
MOD_CK1_1	53	59	PF00069	0.685
MOD_CK1_1	559	565	PF00069	0.412
MOD_CK1_1	585	591	PF00069	0.291
MOD_CK1_1	663	669	PF00069	0.412
MOD_CK1_1	747	753	PF00069	0.606
MOD_CK1_1	95	101	PF00069	0.548
MOD_CK1_1	975	981	PF00069	0.710
MOD_CK2_1	136	142	PF00069	0.631
MOD_CK2_1	5	11	PF00069	0.449
MOD_CK2_1	551	557	PF00069	0.346
MOD_CK2_1	575	581	PF00069	0.373
MOD_CK2_1	732	738	PF00069	0.676
MOD_CK2_1	981	987	PF00069	0.527
MOD_DYRK1A_RPxSP_1	238	242	PF00069	0.498
MOD_GlcNHglycan	1030	1033	PF01048	0.727
MOD_GlcNHglycan	167	170	PF01048	0.662
MOD_GlcNHglycan	187	190	PF01048	0.484
MOD_GlcNHglycan	22	25	PF01048	0.668
MOD_GlcNHglycan	227	230	PF01048	0.561
MOD_GlcNHglycan	258	261	PF01048	0.619
MOD_GlcNHglycan	296	299	PF01048	0.373
MOD_GlcNHglycan	35	38	PF01048	0.517
MOD_GlcNHglycan	353	356	PF01048	0.258
MOD_GlcNHglycan	389	392	PF01048	0.345
MOD_GlcNHglycan	395	398	PF01048	0.281
MOD_GlcNHglycan	414	417	PF01048	0.180
MOD_GlcNHglycan	486	489	PF01048	0.417
MOD_GlcNHglycan	499	502	PF01048	0.440
MOD_GlcNHglycan	53	56	PF01048	0.719
MOD_GlcNHglycan	552	556	PF01048	0.364
MOD_GlcNHglycan	562	565	PF01048	0.181
MOD_GlcNHglycan	623	626	PF01048	0.320
MOD_GlcNHglycan	665	668	PF01048	0.384
MOD_GlcNHglycan	726	729	PF01048	0.724
MOD_GlcNHglycan	742	745	PF01048	0.557
MOD_GlcNHglycan	793	796	PF01048	0.663
MOD_GlcNHglycan	797	800	PF01048	0.624
MOD_GlcNHglycan	801	804	PF01048	0.557
MOD_GlcNHglycan	880	883	PF01048	0.299
MOD_GlcNHglycan	893	896	PF01048	0.270
MOD_GlcNHglycan	94	97	PF01048	0.566
MOD_GlcNHglycan	983	986	PF01048	0.517
MOD_GSK3_1	1018	1025	PF00069	0.631
MOD_GSK3_1	1028	1035	PF00069	0.604
MOD_GSK3_1	114	121	PF00069	0.640
MOD_GSK3_1	150	157	PF00069	0.627
MOD_GSK3_1	163	170	PF00069	0.482
MOD_GSK3_1	213	220	PF00069	0.561
MOD_GSK3_1	225	232	PF00069	0.656
MOD_GSK3_1	243	250	PF00069	0.556
MOD_GSK3_1	306	313	PF00069	0.279
MOD_GSK3_1	326	333	PF00069	0.157
MOD_GSK3_1	33	40	PF00069	0.549
MOD_GSK3_1	493	500	PF00069	0.358
MOD_GSK3_1	556	563	PF00069	0.341
MOD_GSK3_1	582	589	PF00069	0.293
MOD_GSK3_1	609	616	PF00069	0.430
MOD_GSK3_1	685	692	PF00069	0.349
MOD_GSK3_1	696	703	PF00069	0.373
MOD_GSK3_1	718	725	PF00069	0.543
MOD_GSK3_1	740	747	PF00069	0.729
MOD_GSK3_1	791	798	PF00069	0.737
MOD_GSK3_1	815	822	PF00069	0.478
MOD_GSK3_1	901	908	PF00069	0.365
MOD_GSK3_1	94	101	PF00069	0.547
MOD_GSK3_1	956	963	PF00069	0.226
MOD_LATS_1	41	47	PF00433	0.480
MOD_LATS_1	453	459	PF00433	0.279
MOD_N-GLC_1	393	398	PF02516	0.302
MOD_N-GLC_1	613	618	PF02516	0.435
MOD_N-GLC_1	700	705	PF02516	0.346
MOD_N-GLC_1	795	800	PF02516	0.714
MOD_NEK2_1	1028	1033	PF00069	0.666
MOD_NEK2_1	163	168	PF00069	0.584
MOD_NEK2_1	231	236	PF00069	0.723
MOD_NEK2_1	294	299	PF00069	0.373
MOD_NEK2_1	406	411	PF00069	0.285
MOD_NEK2_1	412	417	PF00069	0.276
MOD_NEK2_1	575	580	PF00069	0.341
MOD_NEK2_1	586	591	PF00069	0.274
MOD_NEK2_1	638	643	PF00069	0.357
MOD_NEK2_1	716	721	PF00069	0.280
MOD_NEK2_1	849	854	PF00069	0.440
MOD_NEK2_1	981	986	PF00069	0.465
MOD_NEK2_2	448	453	PF00069	0.314
MOD_PIKK_1	297	303	PF00454	0.346
MOD_PIKK_1	718	724	PF00454	0.322
MOD_PIKK_1	732	738	PF00454	0.649
MOD_PKA_2	20	26	PF00069	0.643
MOD_PKA_2	37	43	PF00069	0.458
MOD_PKA_2	406	412	PF00069	0.186
MOD_PKA_2	454	460	PF00069	0.291
MOD_PKA_2	559	565	PF00069	0.454
MOD_PKA_2	716	722	PF00069	0.346
MOD_PKA_2	776	782	PF00069	0.717
MOD_PKA_2	943	949	PF00069	0.330
MOD_Plk_1	522	528	PF00069	0.373
MOD_Plk_1	679	685	PF00069	0.346
MOD_Plk_1	700	706	PF00069	0.346
MOD_Plk_2-3	306	312	PF00069	0.279
MOD_Plk_4	115	121	PF00069	0.619
MOD_Plk_4	455	461	PF00069	0.279
MOD_Plk_4	5	11	PF00069	0.580
MOD_Plk_4	522	528	PF00069	0.323
MOD_Plk_4	660	666	PF00069	0.444
MOD_Plk_4	689	695	PF00069	0.405
MOD_Plk_4	905	911	PF00069	0.339
MOD_Plk_4	975	981	PF00069	0.697
MOD_ProDKin_1	1018	1024	PF00069	0.520
MOD_ProDKin_1	126	132	PF00069	0.603
MOD_ProDKin_1	238	244	PF00069	0.657
MOD_ProDKin_1	251	257	PF00069	0.730
MOD_ProDKin_1	259	265	PF00069	0.586
MOD_ProDKin_1	358	364	PF00069	0.460
MOD_ProDKin_1	616	622	PF00069	0.417
MOD_ProDKin_1	748	754	PF00069	0.594
MOD_ProDKin_1	770	776	PF00069	0.737
MOD_ProDKin_1	777	783	PF00069	0.681
MOD_ProDKin_1	95	101	PF00069	0.563
MOD_ProDKin_1	972	978	PF00069	0.674
MOD_SUMO_for_1	896	899	PF00179	0.258
MOD_SUMO_rev_2	476	486	PF00179	0.346
TRG_DiLeu_BaEn_1	711	716	PF01217	0.373
TRG_DiLeu_BaEn_4	581	587	PF01217	0.373
TRG_DiLeu_BaLyEn_6	408	413	PF01217	0.313
TRG_DiLeu_BaLyEn_6	570	575	PF01217	0.186
TRG_ENDOCYTIC_2	382	385	PF00928	0.373
TRG_ENDOCYTIC_2	470	473	PF00928	0.279
TRG_ENDOCYTIC_2	916	919	PF00928	0.279
TRG_ER_diArg_1	273	276	PF00400	0.273
TRG_ER_diArg_1	534	536	PF00400	0.346
TRG_ER_diArg_1	76	78	PF00400	0.594
TRG_ER_diArg_1	765	768	PF00400	0.598
TRG_ER_diArg_1	942	945	PF00400	0.186
TRG_NLS_MonoExtC_3	41	47	PF00514	0.566
TRG_NLS_MonoExtN_4	39	46	PF00514	0.655
TRG_Pf-PMV_PEXEL_1	467	471	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	536	540	PF00026	0.186

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEH2	Leptomonas seymouri	44%	100%
A0A3R7MNQ1	Trypanosoma rangeli	40%	100%
A0A3S7WQ47	Leishmania donovani	86%	96%
A4HTH3	Leishmania infantum	86%	96%
C9ZPL4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
Q4QIE7	Leishmania major	88%	100%