Endo/exonuclease/phosphatase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Inositol phosphosphingolipids phospholipase C

Species:

Leishmania mexicana

UniProt:

E9AM99_LEIMU

TriTrypDb:

LmxM.08.0200

Length:

645

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	1
GO:0005783	endoplasmic reticulum	5	1
GO:0016020	membrane	2	5
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0071944	cell periphery	2	1
GO:0110165	cellular anatomical entity	1	5

Expansion

Sequence features

E9AM99

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AM99

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	1
GO:0006643	membrane lipid metabolic process	4	1
GO:0006644	phospholipid metabolic process	4	1
GO:0006665	sphingolipid metabolic process	4	1
GO:0006672	ceramide metabolic process	4	1
GO:0006684	sphingomyelin metabolic process	4	1
GO:0006685	sphingomyelin catabolic process	5	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0008610	lipid biosynthetic process	4	1
GO:0009056	catabolic process	2	1
GO:0009058	biosynthetic process	2	1
GO:0009268	response to pH	3	1
GO:0009395	phospholipid catabolic process	5	1
GO:0009628	response to abiotic stimulus	2	1
GO:0009987	cellular process	1	1
GO:0010447	response to acidic pH	4	1
GO:0016042	lipid catabolic process	4	1
GO:0019637	organophosphate metabolic process	3	1
GO:0030148	sphingolipid biosynthetic process	5	1
GO:0030149	sphingolipid catabolic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043603	amide metabolic process	3	1
GO:0043604	amide biosynthetic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044242	cellular lipid catabolic process	4	1
GO:0044248	cellular catabolic process	3	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046434	organophosphate catabolic process	4	1
GO:0046466	membrane lipid catabolic process	5	1
GO:0046467	membrane lipid biosynthetic process	4	1
GO:0046513	ceramide biosynthetic process	5	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0071214	cellular response to abiotic stimulus	3	1
GO:0071467	cellular response to pH	4	1
GO:0071468	cellular response to acidic pH	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0104004	cellular response to environmental stimulus	3	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901575	organic substance catabolic process	3	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004620	phospholipase activity	5	11
GO:0004767	sphingomyelin phosphodiesterase activity	6	11
GO:0008081	phosphoric diester hydrolase activity	5	11
GO:0016298	lipase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0042578	phosphoric ester hydrolase activity	4	11
GO:0003676	nucleic acid binding	3	3
GO:0003743	translation initiation factor activity	4	3
GO:0005488	binding	1	3
GO:0008135	translation factor activity, RNA binding	3	3
GO:0045182	translation regulator activity	1	3
GO:0090079	translation regulator activity, nucleic acid binding	2	3
GO:0097159	organic cyclic compound binding	2	3
GO:1901363	heterocyclic compound binding	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	402	406	PF00656	0.369
CLV_NRD_NRD_1	76	78	PF00675	0.507
CLV_PCSK_KEX2_1	165	167	PF00082	0.470
CLV_PCSK_KEX2_1	217	219	PF00082	0.543
CLV_PCSK_KEX2_1	343	345	PF00082	0.714
CLV_PCSK_KEX2_1	567	569	PF00082	0.538
CLV_PCSK_KEX2_1	76	78	PF00082	0.476
CLV_PCSK_PC1ET2_1	165	167	PF00082	0.424
CLV_PCSK_PC1ET2_1	217	219	PF00082	0.528
CLV_PCSK_PC1ET2_1	343	345	PF00082	0.714
CLV_PCSK_PC1ET2_1	567	569	PF00082	0.538
CLV_PCSK_SKI1_1	248	252	PF00082	0.580
CLV_PCSK_SKI1_1	255	259	PF00082	0.527
CLV_PCSK_SKI1_1	267	271	PF00082	0.395
CLV_PCSK_SKI1_1	286	290	PF00082	0.526
CLV_PCSK_SKI1_1	361	365	PF00082	0.564
CLV_PCSK_SKI1_1	564	568	PF00082	0.520
DOC_CYCLIN_RxL_1	165	175	PF00134	0.270
DOC_CYCLIN_RxL_1	262	274	PF00134	0.338
DOC_MAPK_gen_1	165	172	PF00069	0.311
DOC_MAPK_gen_1	438	448	PF00069	0.195
DOC_MAPK_MEF2A_6	121	130	PF00069	0.246
DOC_MAPK_MEF2A_6	441	450	PF00069	0.194
DOC_PP1_RVXF_1	13	20	PF00149	0.352
DOC_PP2B_LxvP_1	227	230	PF13499	0.329
DOC_PP4_FxxP_1	284	287	PF00568	0.339
DOC_USP7_MATH_1	257	261	PF00917	0.333
DOC_USP7_MATH_1	410	414	PF00917	0.291
DOC_USP7_MATH_1	536	540	PF00917	0.764
DOC_USP7_MATH_1	547	551	PF00917	0.811
DOC_USP7_UBL2_3	357	361	PF12436	0.568
DOC_WW_Pin1_4	532	537	PF00397	0.665
DOC_WW_Pin1_4	569	574	PF00397	0.695
DOC_WW_Pin1_4	63	68	PF00397	0.270
LIG_14-3-3_CanoR_1	15	20	PF00244	0.343
LIG_14-3-3_CanoR_1	173	180	PF00244	0.246
LIG_14-3-3_CanoR_1	248	253	PF00244	0.262
LIG_14-3-3_CanoR_1	417	423	PF00244	0.412
LIG_14-3-3_CanoR_1	459	467	PF00244	0.481
LIG_14-3-3_CanoR_1	568	573	PF00244	0.793
LIG_14-3-3_CanoR_1	71	76	PF00244	0.358
LIG_14-3-3_CanoR_1	77	83	PF00244	0.346
LIG_Actin_WH2_2	219	236	PF00022	0.270
LIG_ActinCP_TwfCPI_2	284	292	PF01115	0.223
LIG_APCC_ABBA_1	111	116	PF00400	0.326
LIG_APCC_Cbox_1	167	173	PF00515	0.326
LIG_BIR_II_1	1	5	PF00653	0.456
LIG_BRCT_BRCA1_1	380	384	PF00533	0.340
LIG_BRCT_BRCA1_1	605	609	PF00533	0.436
LIG_Clathr_ClatBox_1	268	272	PF01394	0.339
LIG_EH1_1	383	391	PF00400	0.359
LIG_FHA_1	100	106	PF00498	0.231
LIG_FHA_1	146	152	PF00498	0.251
LIG_FHA_1	189	195	PF00498	0.261
LIG_FHA_1	350	356	PF00498	0.560
LIG_FHA_1	485	491	PF00498	0.724
LIG_FHA_1	516	522	PF00498	0.664
LIG_FHA_1	610	616	PF00498	0.414
LIG_FHA_2	131	137	PF00498	0.270
LIG_FHA_2	249	255	PF00498	0.235
LIG_FHA_2	317	323	PF00498	0.366
LIG_FHA_2	37	43	PF00498	0.307
LIG_FHA_2	410	416	PF00498	0.337
LIG_FHA_2	419	425	PF00498	0.275
LIG_FHA_2	481	487	PF00498	0.562
LIG_FHA_2	569	575	PF00498	0.625
LIG_HCF-1_HBM_1	474	477	PF13415	0.471
LIG_LIR_Apic_2	321	327	PF02991	0.247
LIG_LIR_Gen_1	179	185	PF02991	0.289
LIG_LIR_Gen_1	334	342	PF02991	0.478
LIG_LIR_Gen_1	42	49	PF02991	0.326
LIG_LIR_Gen_1	439	450	PF02991	0.344
LIG_LIR_Gen_1	56	65	PF02991	0.259
LIG_LIR_Nem_3	167	172	PF02991	0.244
LIG_LIR_Nem_3	179	183	PF02991	0.301
LIG_LIR_Nem_3	263	269	PF02991	0.332
LIG_LIR_Nem_3	329	335	PF02991	0.512
LIG_LIR_Nem_3	42	47	PF02991	0.326
LIG_LIR_Nem_3	439	445	PF02991	0.344
LIG_LIR_Nem_3	56	61	PF02991	0.259
LIG_LIR_Nem_3	586	590	PF02991	0.568
LIG_LIR_Nem_3	72	78	PF02991	0.202
LIG_NRBOX	445	451	PF00104	0.175
LIG_Pex14_2	58	62	PF04695	0.380
LIG_Pex14_2	78	82	PF04695	0.190
LIG_Pex14_2	99	103	PF04695	0.307
LIG_Rb_pABgroove_1	471	479	PF01858	0.469
LIG_REV1ctd_RIR_1	115	125	PF16727	0.339
LIG_REV1ctd_RIR_1	281	286	PF16727	0.270
LIG_SH2_CRK	44	48	PF00017	0.246
LIG_SH2_CRK	442	446	PF00017	0.370
LIG_SH2_NCK_1	155	159	PF00017	0.270
LIG_SH2_NCK_1	477	481	PF00017	0.569
LIG_SH2_PTP2	324	327	PF00017	0.292
LIG_SH2_SRC	324	327	PF00017	0.296
LIG_SH2_STAP1	147	151	PF00017	0.255
LIG_SH2_STAP1	44	48	PF00017	0.246
LIG_SH2_STAP1	477	481	PF00017	0.606
LIG_SH2_STAP1	600	604	PF00017	0.658
LIG_SH2_STAT5	147	150	PF00017	0.302
LIG_SH2_STAT5	169	172	PF00017	0.231
LIG_SH2_STAT5	265	268	PF00017	0.376
LIG_SH2_STAT5	324	327	PF00017	0.426
LIG_SH2_STAT5	429	432	PF00017	0.346
LIG_SH2_STAT5	73	76	PF00017	0.257
LIG_SH3_3	125	131	PF00018	0.311
LIG_SH3_3	223	229	PF00018	0.266
LIG_SH3_3	320	326	PF00018	0.338
LIG_SH3_3	543	549	PF00018	0.795
LIG_SUMO_SIM_anti_2	447	452	PF11976	0.436
LIG_SUMO_SIM_anti_2	45	51	PF11976	0.246
LIG_SUMO_SIM_par_1	148	156	PF11976	0.325
LIG_SUMO_SIM_par_1	45	51	PF11976	0.246
LIG_TRAF2_1	365	368	PF00917	0.356
LIG_TRAF2_1	463	466	PF00917	0.603
LIG_TYR_ITSM	438	445	PF00017	0.329
LIG_UBA3_1	211	217	PF00899	0.304
LIG_WRC_WIRS_1	16	21	PF05994	0.262
LIG_WRC_WIRS_1	4	9	PF05994	0.416
LIG_WW_3	228	232	PF00397	0.326
MOD_CK1_1	159	165	PF00069	0.326
MOD_CK1_1	188	194	PF00069	0.266
MOD_CK1_1	2	8	PF00069	0.432
MOD_CK1_1	203	209	PF00069	0.317
MOD_CK1_1	260	266	PF00069	0.371
MOD_CK1_1	400	406	PF00069	0.503
MOD_CK2_1	316	322	PF00069	0.402
MOD_CK2_1	36	42	PF00069	0.335
MOD_CK2_1	362	368	PF00069	0.456
MOD_CK2_1	396	402	PF00069	0.442
MOD_CK2_1	409	415	PF00069	0.338
MOD_CK2_1	459	465	PF00069	0.633
MOD_CK2_1	568	574	PF00069	0.685
MOD_GlcNHglycan	135	140	PF01048	0.513
MOD_GlcNHglycan	357	360	PF01048	0.799
MOD_GlcNHglycan	367	372	PF01048	0.703
MOD_GlcNHglycan	399	402	PF01048	0.717
MOD_GlcNHglycan	461	464	PF01048	0.482
MOD_GlcNHglycan	468	471	PF01048	0.472
MOD_GlcNHglycan	53	56	PF01048	0.588
MOD_GlcNHglycan	606	609	PF01048	0.334
MOD_GlcNHglycan	84	87	PF01048	0.446
MOD_GSK3_1	142	149	PF00069	0.295
MOD_GSK3_1	156	163	PF00069	0.241
MOD_GSK3_1	172	179	PF00069	0.263
MOD_GSK3_1	184	191	PF00069	0.270
MOD_GSK3_1	257	264	PF00069	0.299
MOD_GSK3_1	327	334	PF00069	0.351
MOD_GSK3_1	355	362	PF00069	0.477
MOD_GSK3_1	363	370	PF00069	0.470
MOD_GSK3_1	393	400	PF00069	0.444
MOD_GSK3_1	432	439	PF00069	0.400
MOD_GSK3_1	480	487	PF00069	0.609
MOD_GSK3_1	532	539	PF00069	0.719
MOD_GSK3_1	553	560	PF00069	0.759
MOD_GSK3_1	599	606	PF00069	0.629
MOD_GSK3_1	78	85	PF00069	0.347
MOD_N-GLC_1	200	205	PF02516	0.459
MOD_N-GLC_1	349	354	PF02516	0.758
MOD_N-GLC_1	609	614	PF02516	0.318
MOD_NEK2_1	146	151	PF00069	0.269
MOD_NEK2_1	172	177	PF00069	0.326
MOD_NEK2_1	183	188	PF00069	0.302
MOD_NEK2_1	200	205	PF00069	0.259
MOD_NEK2_1	261	266	PF00069	0.313
MOD_NEK2_1	355	360	PF00069	0.476
MOD_NEK2_1	599	604	PF00069	0.656
MOD_NEK2_1	609	614	PF00069	0.425
MOD_NEK2_1	78	83	PF00069	0.316
MOD_NEK2_1	99	104	PF00069	0.306
MOD_NEK2_2	142	147	PF00069	0.168
MOD_NEK2_2	257	262	PF00069	0.168
MOD_NEK2_2	496	501	PF00069	0.622
MOD_NEK2_2	515	520	PF00069	0.535
MOD_PIKK_1	156	162	PF00454	0.302
MOD_PIKK_1	172	178	PF00454	0.246
MOD_PIKK_1	200	206	PF00454	0.260
MOD_PIKK_1	274	280	PF00454	0.326
MOD_PIKK_1	432	438	PF00454	0.394
MOD_PKA_1	567	573	PF00069	0.726
MOD_PKA_2	172	178	PF00069	0.246
MOD_PKA_2	188	194	PF00069	0.339
MOD_PKA_2	484	490	PF00069	0.674
MOD_PKA_2	567	573	PF00069	0.726
MOD_Plk_4	142	148	PF00069	0.304
MOD_Plk_4	222	228	PF00069	0.286
MOD_Plk_4	261	267	PF00069	0.334
MOD_Plk_4	327	333	PF00069	0.489
MOD_Plk_4	418	424	PF00069	0.352
MOD_Plk_4	444	450	PF00069	0.307
MOD_Plk_4	600	606	PF00069	0.570
MOD_Plk_4	78	84	PF00069	0.348
MOD_ProDKin_1	532	538	PF00069	0.665
MOD_ProDKin_1	569	575	PF00069	0.694
MOD_ProDKin_1	63	69	PF00069	0.270
MOD_SUMO_for_1	269	272	PF00179	0.326
MOD_SUMO_for_1	463	466	PF00179	0.635
TRG_DiLeu_BaEn_1	56	61	PF01217	0.339
TRG_ENDOCYTIC_2	155	158	PF00928	0.315
TRG_ENDOCYTIC_2	169	172	PF00928	0.243
TRG_ENDOCYTIC_2	265	268	PF00928	0.320
TRG_ENDOCYTIC_2	332	335	PF00928	0.423
TRG_ENDOCYTIC_2	377	380	PF00928	0.370
TRG_ENDOCYTIC_2	44	47	PF00928	0.246
TRG_ENDOCYTIC_2	442	445	PF00928	0.357
TRG_ER_diArg_1	75	77	PF00400	0.324
TRG_Pf-PMV_PEXEL_1	205	210	PF00026	0.459
TRG_Pf-PMV_PEXEL_1	231	235	PF00026	0.539
TRG_Pf-PMV_PEXEL_1	588	592	PF00026	0.475

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P935	Leptomonas seymouri	57%	100%
A0A1X0NNY2	Trypanosomatidae	35%	100%
A0A3S5H634	Leishmania donovani	89%	99%
A0A422NTR5	Trypanosoma rangeli	36%	100%
A4H587	Leishmania braziliensis	71%	100%
A4HTH1	Leishmania infantum	89%	99%
C9ZPP9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
Q4QIE9	Leishmania major	86%	99%
V5BLM5	Trypanosoma cruzi	37%	100%