Tryptophan--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tryptophanyl-tRNA synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9AM73_LEIMU

TriTrypDb:

LmxM.08_29.0060

Length:

396

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AM73

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AM73

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006436	tryptophanyl-tRNA aminoacylation	7	12
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004830	tryptophan-tRNA ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	367	369	PF00675	0.419
CLV_NRD_NRD_1	55	57	PF00675	0.325
CLV_PCSK_KEX2_1	333	335	PF00082	0.296
CLV_PCSK_KEX2_1	55	57	PF00082	0.344
CLV_PCSK_PC1ET2_1	333	335	PF00082	0.296
CLV_PCSK_SKI1_1	122	126	PF00082	0.281
CLV_PCSK_SKI1_1	196	200	PF00082	0.237
CLV_PCSK_SKI1_1	24	28	PF00082	0.412
CLV_PCSK_SKI1_1	285	289	PF00082	0.247
CLV_PCSK_SKI1_1	293	297	PF00082	0.247
CLV_PCSK_SKI1_1	323	327	PF00082	0.333
CLV_PCSK_SKI1_1	343	347	PF00082	0.116
CLV_PCSK_SKI1_1	350	354	PF00082	0.241
CLV_PCSK_SKI1_1	369	373	PF00082	0.324
CLV_PCSK_SKI1_1	56	60	PF00082	0.327
DEG_Nend_UBRbox_2	1	3	PF02207	0.648
DOC_AGCK_PIF_2	157	162	PF00069	0.447
DOC_CKS1_1	10	15	PF01111	0.436
DOC_MAPK_gen_1	303	309	PF00069	0.552
DOC_MAPK_MEF2A_6	142	149	PF00069	0.396
DOC_MAPK_NFAT4_5	142	150	PF00069	0.425
DOC_MIT_MIM_1	35	45	PF04212	0.232
DOC_PP1_RVXF_1	150	156	PF00149	0.496
DOC_PP1_RVXF_1	54	61	PF00149	0.327
DOC_PP4_FxxP_1	109	112	PF00568	0.447
DOC_USP7_MATH_1	204	208	PF00917	0.447
DOC_USP7_UBL2_3	122	126	PF12436	0.507
DOC_USP7_UBL2_3	333	337	PF12436	0.496
DOC_WW_Pin1_4	280	285	PF00397	0.533
DOC_WW_Pin1_4	9	14	PF00397	0.441
LIG_14-3-3_CanoR_1	368	378	PF00244	0.434
LIG_14-3-3_CanoR_1	84	93	PF00244	0.447
LIG_Actin_WH2_2	126	144	PF00022	0.533
LIG_Actin_WH2_2	236	253	PF00022	0.447
LIG_BRCT_BRCA1_1	208	212	PF00533	0.447
LIG_BRCT_BRCA1_1	256	260	PF00533	0.447
LIG_FHA_1	100	106	PF00498	0.441
LIG_FHA_1	136	142	PF00498	0.447
LIG_FHA_1	238	244	PF00498	0.446
LIG_FHA_1	344	350	PF00498	0.453
LIG_FHA_1	356	362	PF00498	0.433
LIG_FHA_2	116	122	PF00498	0.447
LIG_FHA_2	127	133	PF00498	0.447
LIG_FHA_2	311	317	PF00498	0.483
LIG_FHA_2	370	376	PF00498	0.399
LIG_LIR_Apic_2	107	112	PF02991	0.447
LIG_LIR_Gen_1	159	167	PF02991	0.471
LIG_LIR_Gen_1	19	29	PF02991	0.416
LIG_LIR_Gen_1	377	387	PF02991	0.507
LIG_LIR_Nem_3	154	160	PF02991	0.447
LIG_LIR_Nem_3	19	25	PF02991	0.419
LIG_LIR_Nem_3	209	215	PF02991	0.447
LIG_LIR_Nem_3	234	239	PF02991	0.458
LIG_LIR_Nem_3	257	263	PF02991	0.447
LIG_LIR_Nem_3	377	382	PF02991	0.506
LIG_MLH1_MIPbox_1	256	260	PF16413	0.447
LIG_NRP_CendR_1	395	396	PF00754	0.413
LIG_Pex14_1	321	325	PF04695	0.447
LIG_Pex14_2	124	128	PF04695	0.343
LIG_Pex14_2	208	212	PF04695	0.447
LIG_REV1ctd_RIR_1	121	130	PF16727	0.487
LIG_REV1ctd_RIR_1	57	67	PF16727	0.329
LIG_SH2_CRK	167	171	PF00017	0.447
LIG_SH2_CRK	22	26	PF00017	0.401
LIG_SH2_STAT5	162	165	PF00017	0.507
LIG_SH2_STAT5	177	180	PF00017	0.507
LIG_SH2_STAT5	236	239	PF00017	0.447
LIG_SH2_STAT5	249	252	PF00017	0.447
LIG_SH2_STAT5	79	82	PF00017	0.447
LIG_SH3_3	312	318	PF00018	0.447
LIG_SH3_3	381	387	PF00018	0.407
LIG_SH3_3	7	13	PF00018	0.465
LIG_TRAF2_1	129	132	PF00917	0.489
LIG_TRAF2_1	363	366	PF00917	0.449
LIG_TRAF2_1	388	391	PF00917	0.501
LIG_TRAF2_1	5	8	PF00917	0.681
LIG_TYR_ITIM	20	25	PF00017	0.412
LIG_UBA3_1	144	152	PF00899	0.425
LIG_UBA3_1	96	103	PF00899	0.458
MOD_CDK_SPK_2	280	285	PF00069	0.533
MOD_CK1_1	271	277	PF00069	0.458
MOD_CK1_1	87	93	PF00069	0.454
MOD_CK2_1	115	121	PF00069	0.441
MOD_CK2_1	126	132	PF00069	0.429
MOD_Cter_Amidation	46	49	PF01082	0.313
MOD_GlcNHglycan	208	211	PF01048	0.288
MOD_GlcNHglycan	263	266	PF01048	0.247
MOD_GlcNHglycan	296	299	PF01048	0.247
MOD_GSK3_1	355	362	PF00069	0.533
MOD_NEK2_1	105	110	PF00069	0.447
MOD_NEK2_1	263	268	PF00069	0.455
MOD_NEK2_1	270	275	PF00069	0.445
MOD_NEK2_1	99	104	PF00069	0.447
MOD_PIKK_1	177	183	PF00454	0.447
MOD_PKA_1	369	375	PF00069	0.490
MOD_PKA_2	237	243	PF00069	0.447
MOD_Plk_1	126	132	PF00069	0.473
MOD_Plk_4	151	157	PF00069	0.447
MOD_ProDKin_1	280	286	PF00069	0.533
MOD_ProDKin_1	9	15	PF00069	0.442
MOD_SUMO_for_1	188	191	PF00179	0.456
MOD_SUMO_for_1	388	391	PF00179	0.501
MOD_SUMO_rev_2	119	124	PF00179	0.447
MOD_SUMO_rev_2	327	335	PF00179	0.530
TRG_DiLeu_BaEn_4	330	336	PF01217	0.533
TRG_ENDOCYTIC_2	167	170	PF00928	0.447
TRG_ENDOCYTIC_2	22	25	PF00928	0.409
TRG_ENDOCYTIC_2	236	239	PF00928	0.437
TRG_ER_diArg_1	54	56	PF00400	0.329
TRG_NLS_Bipartite_1	350	372	PF00514	0.582
TRG_NLS_MonoExtC_3	367	373	PF00514	0.486

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3G3	Leptomonas seymouri	86%	100%
A0A0N1HXL2	Leptomonas seymouri	39%	84%
A0A0S4IN16	Bodo saltans	75%	100%
A0A0S4JC70	Bodo saltans	44%	87%
A0A1X0NGJ6	Trypanosomatidae	40%	86%
A0A1X0NZB4	Trypanosomatidae	79%	100%
A0A3R7MPN7	Trypanosoma rangeli	79%	100%
A0A3S7WXL2	Leishmania donovani	44%	96%
A0A3S7X238	Leishmania donovani	96%	100%
A0A422NW64	Trypanosoma rangeli	42%	89%
A2BLD4	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	31%	100%
A3MX72	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	39%	100%
A4HCP4	Leishmania braziliensis	38%	100%
A4HH14	Leishmania braziliensis	91%	100%
A4I070	Leishmania infantum	44%	96%
A4I443	Leishmania infantum	96%	100%
A4WL99	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	36%	100%
B6YUH1	Thermococcus onnurineus (strain NA1)	46%	100%
C6A032	Thermococcus sibiricus (strain DSM 12597 / MM 739)	46%	100%
C9ZKR9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	79%	100%
E9ADK8	Leishmania major	96%	100%
E9AW33	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	96%
O26352	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	29%	100%
O59584	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	45%	100%
O96771	Encephalitozoon cuniculi (strain GB-M1)	43%	100%
P17248	Bos taurus	55%	83%
P23381	Homo sapiens	56%	84%
P23612	Oryctolagus cuniculus	55%	83%
P32921	Mus musculus	56%	82%
Q09692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	56%	100%
Q12109	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	50%	92%
Q4JBG7	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	47%	100%
Q4QBE4	Leishmania major	41%	100%
Q55DZ8	Dictyostelium discoideum	54%	99%
Q58810	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	29%	100%
Q5JEP3	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	45%	100%
Q5R4J1	Pongo abelii	55%	84%
Q6P7B0	Rattus norvegicus	56%	82%
Q8TYF7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	32%	100%
Q8U453	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	47%	100%
Q8ZTU5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	37%	100%
Q976M1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	46%	100%
Q97ZX0	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	48%	100%
Q9HN66	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	35%	100%
Q9SR15	Arabidopsis thaliana	56%	99%
Q9UY11	Pyrococcus abyssi (strain GE5 / Orsay)	45%	100%
Q9Y924	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	32%	100%
V5B4S5	Trypanosoma cruzi	80%	100%