Non-specific serine/threonine protein kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

serine/threonine protein kinase RIO1, putative

Species:

Leishmania mexicana

UniProt:

E9AM55_LEIMU

TriTrypDb:

LmxM.08_29.0240

Length:

537

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AM55

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AM55

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	5
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0006807	nitrogen compound metabolic process	2	5
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016310	phosphorylation	5	12
GO:0019538	protein metabolic process	3	5
GO:0036211	protein modification process	4	5
GO:0043170	macromolecule metabolic process	3	5
GO:0043412	macromolecule modification	4	5
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	5
GO:0071704	organic substance metabolic process	2	5
GO:1901564	organonitrogen compound metabolic process	3	5
GO:0022613	ribonucleoprotein complex biogenesis	4	1
GO:0042254	ribosome biogenesis	5	1
GO:0044085	cellular component biogenesis	3	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004672	protein kinase activity	3	12
GO:0004674	protein serine/threonine kinase activity	4	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0106310	protein serine kinase activity	4	5
GO:0140096	catalytic activity, acting on a protein	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	397	401	PF00656	0.473
CLV_C14_Caspase3-7	464	468	PF00656	0.778
CLV_C14_Caspase3-7	482	486	PF00656	0.710
CLV_MEL_PAP_1	434	440	PF00089	0.362
CLV_NRD_NRD_1	222	224	PF00675	0.222
CLV_NRD_NRD_1	295	297	PF00675	0.209
CLV_NRD_NRD_1	342	344	PF00675	0.198
CLV_NRD_NRD_1	505	507	PF00675	0.456
CLV_NRD_NRD_1	509	511	PF00675	0.468
CLV_NRD_NRD_1	530	532	PF00675	0.536
CLV_NRD_NRD_1	535	537	PF00675	0.617
CLV_PCSK_FUR_1	518	522	PF00082	0.449
CLV_PCSK_KEX2_1	342	344	PF00082	0.231
CLV_PCSK_KEX2_1	520	522	PF00082	0.444
CLV_PCSK_PC1ET2_1	520	522	PF00082	0.567
CLV_PCSK_SKI1_1	117	121	PF00082	0.481
CLV_PCSK_SKI1_1	151	155	PF00082	0.330
CLV_PCSK_SKI1_1	2	6	PF00082	0.598
CLV_PCSK_SKI1_1	274	278	PF00082	0.290
CLV_PCSK_SKI1_1	355	359	PF00082	0.311
CLV_PCSK_SKI1_1	511	515	PF00082	0.444
DEG_APCC_DBOX_1	150	158	PF00400	0.306
DEG_Nend_UBRbox_1	1	4	PF02207	0.554
DEG_ODPH_VHL_1	246	259	PF01847	0.409
DOC_MAPK_gen_1	192	201	PF00069	0.398
DOC_MAPK_gen_1	232	240	PF00069	0.500
DOC_MAPK_gen_1	251	259	PF00069	0.323
DOC_MAPK_gen_1	318	326	PF00069	0.406
DOC_MAPK_gen_1	342	348	PF00069	0.409
DOC_MAPK_MEF2A_6	318	326	PF00069	0.393
DOC_MAPK_MEF2A_6	416	425	PF00069	0.379
DOC_PP1_RVXF_1	272	279	PF00149	0.398
DOC_PP4_FxxP_1	35	38	PF00568	0.616
DOC_USP7_MATH_1	374	378	PF00917	0.434
DOC_USP7_MATH_1	64	68	PF00917	0.631
DOC_USP7_UBL2_3	282	286	PF12436	0.485
DOC_USP7_UBL2_3	503	507	PF12436	0.508
DOC_USP7_UBL2_3	520	524	PF12436	0.490
DOC_USP7_UBL2_3	533	537	PF12436	0.477
DOC_WW_Pin1_4	184	189	PF00397	0.465
DOC_WW_Pin1_4	483	488	PF00397	0.635
LIG_14-3-3_CanoR_1	139	143	PF00244	0.472
LIG_14-3-3_CanoR_1	336	341	PF00244	0.415
LIG_APCC_ABBA_1	444	449	PF00400	0.539
LIG_BRCT_BRCA1_1	121	125	PF00533	0.619
LIG_BRCT_BRCA1_1	208	212	PF00533	0.465
LIG_BRCT_BRCA1_1	336	340	PF00533	0.485
LIG_eIF4E_1	299	305	PF01652	0.423
LIG_FHA_1	148	154	PF00498	0.327
LIG_FHA_1	194	200	PF00498	0.418
LIG_FHA_2	16	22	PF00498	0.491
LIG_FHA_2	170	176	PF00498	0.342
LIG_FHA_2	226	232	PF00498	0.398
LIG_FHA_2	500	506	PF00498	0.478
LIG_LIR_Apic_2	265	271	PF02991	0.399
LIG_LIR_Apic_2	32	38	PF02991	0.621
LIG_LIR_Apic_2	329	335	PF02991	0.447
LIG_LIR_Gen_1	285	295	PF02991	0.398
LIG_LIR_Gen_1	310	317	PF02991	0.410
LIG_LIR_Gen_1	365	375	PF02991	0.330
LIG_LIR_Gen_1	419	428	PF02991	0.417
LIG_LIR_Nem_3	211	217	PF02991	0.398
LIG_LIR_Nem_3	28	34	PF02991	0.549
LIG_LIR_Nem_3	285	291	PF02991	0.398
LIG_LIR_Nem_3	310	315	PF02991	0.410
LIG_LIR_Nem_3	350	354	PF02991	0.326
LIG_LIR_Nem_3	365	370	PF02991	0.313
LIG_LIR_Nem_3	419	425	PF02991	0.405
LIG_REV1ctd_RIR_1	349	359	PF16727	0.398
LIG_RPA_C_Insects	521	536	PF08784	0.547
LIG_SH2_CRK	12	16	PF00017	0.453
LIG_SH2_CRK	155	159	PF00017	0.313
LIG_SH2_CRK	312	316	PF00017	0.250
LIG_SH2_SRC	332	335	PF00017	0.302
LIG_SH2_STAP1	31	35	PF00017	0.629
LIG_SH2_STAT5	207	210	PF00017	0.237
LIG_SH2_STAT5	217	220	PF00017	0.234
LIG_SH2_STAT5	288	291	PF00017	0.234
LIG_SH2_STAT5	34	37	PF00017	0.606
LIG_SH3_3	142	148	PF00018	0.405
LIG_SH3_3	242	248	PF00018	0.248
LIG_SH3_3	273	279	PF00018	0.234
LIG_SH3_3	481	487	PF00018	0.566
LIG_SUMO_SIM_par_1	320	325	PF11976	0.234
LIG_TRAF2_1	502	505	PF00917	0.476
LIG_TYR_ITIM	153	158	PF00017	0.301
LIG_UBA3_1	391	396	PF00899	0.489
LIG_WRC_WIRS_1	198	203	PF05994	0.378
LIG_WRC_WIRS_1	348	353	PF05994	0.313
MOD_CDC14_SPxK_1	486	489	PF00782	0.636
MOD_CDK_SPxK_1	483	489	PF00069	0.636
MOD_CK1_1	123	129	PF00069	0.486
MOD_CK1_1	197	203	PF00069	0.389
MOD_CK1_1	54	60	PF00069	0.593
MOD_CK2_1	169	175	PF00069	0.349
MOD_CK2_1	225	231	PF00069	0.234
MOD_CK2_1	427	433	PF00069	0.510
MOD_CK2_1	499	505	PF00069	0.478
MOD_Cter_Amidation	534	537	PF01082	0.683
MOD_GlcNHglycan	102	105	PF01048	0.532
MOD_GlcNHglycan	159	162	PF01048	0.356
MOD_GlcNHglycan	182	185	PF01048	0.378
MOD_GlcNHglycan	208	211	PF01048	0.327
MOD_GlcNHglycan	279	282	PF01048	0.245
MOD_GlcNHglycan	376	379	PF01048	0.474
MOD_GlcNHglycan	45	48	PF01048	0.608
MOD_GlcNHglycan	461	466	PF01048	0.734
MOD_GlcNHglycan	97	100	PF01048	0.567
MOD_GSK3_1	119	126	PF00069	0.438
MOD_GSK3_1	134	141	PF00069	0.512
MOD_GSK3_1	157	164	PF00069	0.455
MOD_GSK3_1	180	187	PF00069	0.378
MOD_GSK3_1	193	200	PF00069	0.378
MOD_GSK3_1	355	362	PF00069	0.449
MOD_GSK3_1	423	430	PF00069	0.460
MOD_GSK3_1	457	464	PF00069	0.689
MOD_GSK3_1	52	59	PF00069	0.542
MOD_LATS_1	334	340	PF00433	0.257
MOD_N-GLC_1	499	504	PF02516	0.619
MOD_N-GLC_2	167	169	PF02516	0.358
MOD_NEK2_1	119	124	PF00069	0.500
MOD_NEK2_1	157	162	PF00069	0.324
MOD_NEK2_1	225	230	PF00069	0.234
MOD_NEK2_1	291	296	PF00069	0.295
MOD_NEK2_1	423	428	PF00069	0.590
MOD_PIKK_1	123	129	PF00454	0.477
MOD_PIKK_1	324	330	PF00454	0.234
MOD_PKA_2	123	129	PF00069	0.525
MOD_PKA_2	138	144	PF00069	0.479
MOD_PKB_1	353	361	PF00069	0.257
MOD_Plk_1	117	123	PF00069	0.472
MOD_Plk_1	274	280	PF00069	0.304
MOD_Plk_2-3	138	144	PF00069	0.485
MOD_Plk_2-3	476	482	PF00069	0.690
MOD_Plk_4	194	200	PF00069	0.243
MOD_Plk_4	336	342	PF00069	0.393
MOD_Plk_4	347	353	PF00069	0.259
MOD_ProDKin_1	184	190	PF00069	0.327
MOD_ProDKin_1	483	489	PF00069	0.636
MOD_SUMO_for_1	40	43	PF00179	0.590
TRG_DiLeu_BaEn_1	233	238	PF01217	0.378
TRG_DiLeu_BaEn_1	311	316	PF01217	0.293
TRG_DiLeu_BaEn_2	442	448	PF01217	0.481
TRG_DiLeu_BaLyEn_6	148	153	PF01217	0.342
TRG_ENDOCYTIC_2	155	158	PF00928	0.308
TRG_ENDOCYTIC_2	179	182	PF00928	0.277
TRG_ENDOCYTIC_2	288	291	PF00928	0.234
TRG_ENDOCYTIC_2	312	315	PF00928	0.250
TRG_ER_diArg_1	148	151	PF00400	0.332
TRG_ER_diArg_1	317	320	PF00400	0.282
TRG_ER_diArg_1	341	343	PF00400	0.234
TRG_ER_diArg_1	352	355	PF00400	0.234
TRG_ER_diArg_1	86	89	PF00400	0.456
TRG_ER_diLys_1	532	537	PF00400	0.569
TRG_NLS_Bipartite_1	520	537	PF00514	0.627
TRG_NLS_MonoCore_2	530	535	PF00514	0.572
TRG_NLS_MonoExtC_3	531	536	PF00514	0.562
TRG_NLS_MonoExtN_4	531	537	PF00514	0.580
TRG_Pf-PMV_PEXEL_1	111	115	PF00026	0.458
TRG_Pf-PMV_PEXEL_1	429	433	PF00026	0.440

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIE8	Leptomonas seymouri	82%	97%
A0A0S4JDG4	Bodo saltans	55%	97%
A0A1X0NZG1	Trypanosomatidae	64%	97%
A0A3S7X229	Leishmania donovani	95%	100%
A0A422NUW2	Trypanosoma rangeli	62%	96%
A4HH32	Leishmania braziliensis	88%	100%
A4I477	Leishmania infantum	94%	100%
C9ZKQ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	94%
E9ADM6	Leishmania major	93%	100%
G0S3J5	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	38%	100%
O14730	Homo sapiens	36%	100%
O42650	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	42%	100%
O44959	Caenorhabditis elegans	41%	100%
P34649	Caenorhabditis elegans	35%	100%
Q12196	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	100%
Q1RMT7	Bos taurus	36%	100%
Q922Q2	Mus musculus	41%	95%
Q9BRS2	Homo sapiens	42%	95%
Q9DBU3	Mus musculus	37%	100%
V5BUJ3	Trypanosoma cruzi	63%	99%