Tubulin-tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Tubulin-tyrosine ligase family, putative

Species:

Leishmania mexicana

UniProt:

E9AM52_LEIMU

TriTrypDb:

LmxM.08_29.0260

Length:

595

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0120025	plasma membrane bounded cell projection	3	1

Expansion

Sequence features

E9AM52

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AM52

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0019538	protein metabolic process	3	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0018095	protein polyglutamylation	7	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018200	peptidyl-glutamic acid modification	6	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0008092	cytoskeletal protein binding	3	1
GO:0015631	tubulin binding	4	1
GO:0016874	ligase activity	2	3
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	1
GO:0016881	acid-amino acid ligase activity	4	1
GO:0070739	protein-glutamic acid ligase activity	3	1
GO:0070740	tubulin-glutamic acid ligase activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	121	123	PF00675	0.230
CLV_NRD_NRD_1	128	130	PF00675	0.230
CLV_NRD_NRD_1	188	190	PF00675	0.268
CLV_NRD_NRD_1	233	235	PF00675	0.323
CLV_NRD_NRD_1	255	257	PF00675	0.243
CLV_NRD_NRD_1	276	278	PF00675	0.617
CLV_NRD_NRD_1	413	415	PF00675	0.350
CLV_NRD_NRD_1	432	434	PF00675	0.260
CLV_NRD_NRD_1	549	551	PF00675	0.462
CLV_NRD_NRD_1	592	594	PF00675	0.527
CLV_PCSK_FUR_1	411	415	PF00082	0.394
CLV_PCSK_KEX2_1	128	130	PF00082	0.241
CLV_PCSK_KEX2_1	188	190	PF00082	0.268
CLV_PCSK_KEX2_1	238	240	PF00082	0.315
CLV_PCSK_KEX2_1	276	278	PF00082	0.616
CLV_PCSK_KEX2_1	413	415	PF00082	0.371
CLV_PCSK_KEX2_1	432	434	PF00082	0.253
CLV_PCSK_KEX2_1	549	551	PF00082	0.462
CLV_PCSK_KEX2_1	592	594	PF00082	0.527
CLV_PCSK_PC1ET2_1	238	240	PF00082	0.326
CLV_PCSK_PC7_1	234	240	PF00082	0.326
CLV_PCSK_SKI1_1	122	126	PF00082	0.232
CLV_PCSK_SKI1_1	189	193	PF00082	0.349
CLV_PCSK_SKI1_1	202	206	PF00082	0.320
CLV_PCSK_SKI1_1	249	253	PF00082	0.326
CLV_PCSK_SKI1_1	343	347	PF00082	0.329
CLV_PCSK_SKI1_1	549	553	PF00082	0.425
CLV_PCSK_SKI1_1	80	84	PF00082	0.551
CLV_Separin_Metazoa	305	309	PF03568	0.487
CLV_Separin_Metazoa	429	433	PF03568	0.452
DEG_APCC_DBOX_1	318	326	PF00400	0.501
DEG_Nend_UBRbox_1	1	4	PF02207	0.750
DOC_CKS1_1	81	86	PF01111	0.487
DOC_CYCLIN_RxL_1	162	170	PF00134	0.468
DOC_CYCLIN_yCln2_LP_2	169	175	PF00134	0.514
DOC_CYCLIN_yCln2_LP_2	356	362	PF00134	0.412
DOC_MAPK_DCC_7	462	471	PF00069	0.526
DOC_MAPK_gen_1	234	245	PF00069	0.502
DOC_MAPK_gen_1	256	264	PF00069	0.440
DOC_MAPK_MEF2A_6	238	247	PF00069	0.482
DOC_MAPK_MEF2A_6	256	264	PF00069	0.346
DOC_MAPK_MEF2A_6	308	317	PF00069	0.391
DOC_MAPK_NFAT4_5	257	265	PF00069	0.514
DOC_PP1_RVXF_1	121	128	PF00149	0.441
DOC_PP1_RVXF_1	200	207	PF00149	0.514
DOC_PP1_RVXF_1	49	56	PF00149	0.475
DOC_PP2B_LxvP_1	169	172	PF13499	0.514
DOC_PP2B_LxvP_1	356	359	PF13499	0.514
DOC_PP2B_LxvP_1	403	406	PF13499	0.426
DOC_USP7_MATH_1	19	23	PF00917	0.739
DOC_USP7_MATH_1	195	199	PF00917	0.468
DOC_USP7_MATH_1	294	298	PF00917	0.583
DOC_USP7_MATH_1	395	399	PF00917	0.499
DOC_USP7_MATH_1	488	492	PF00917	0.526
DOC_WW_Pin1_4	221	226	PF00397	0.443
DOC_WW_Pin1_4	473	478	PF00397	0.430
DOC_WW_Pin1_4	74	79	PF00397	0.509
DOC_WW_Pin1_4	80	85	PF00397	0.545
LIG_14-3-3_CanoR_1	220	225	PF00244	0.526
LIG_14-3-3_CanoR_1	276	281	PF00244	0.720
LIG_14-3-3_CanoR_1	286	292	PF00244	0.620
LIG_14-3-3_CanoR_1	411	417	PF00244	0.383
LIG_14-3-3_CanoR_1	539	544	PF00244	0.313
LIG_Actin_WH2_2	448	464	PF00022	0.479
LIG_Actin_WH2_2	482	497	PF00022	0.498
LIG_APCC_ABBA_1	1	6	PF00400	0.548
LIG_APCC_ABBA_1	243	248	PF00400	0.526
LIG_APCC_ABBA_1	315	320	PF00400	0.430
LIG_Clathr_ClatBox_1	454	458	PF01394	0.430
LIG_eIF4E_1	246	252	PF01652	0.495
LIG_FHA_1	154	160	PF00498	0.418
LIG_FHA_1	419	425	PF00498	0.481
LIG_FHA_1	495	501	PF00498	0.526
LIG_FHA_2	210	216	PF00498	0.526
LIG_FHA_2	221	227	PF00498	0.460
LIG_FHA_2	228	234	PF00498	0.373
LIG_FHA_2	481	487	PF00498	0.473
LIG_FHA_2	54	60	PF00498	0.466
LIG_FHA_2	569	575	PF00498	0.595
LIG_LIR_Gen_1	164	173	PF02991	0.546
LIG_LIR_Gen_1	174	181	PF02991	0.495
LIG_LIR_Gen_1	40	50	PF02991	0.351
LIG_LIR_Gen_1	450	460	PF02991	0.528
LIG_LIR_Nem_3	164	169	PF02991	0.546
LIG_LIR_Nem_3	174	178	PF02991	0.495
LIG_LIR_Nem_3	40	46	PF02991	0.362
LIG_LIR_Nem_3	450	455	PF02991	0.514
LIG_LIR_Nem_3	496	501	PF02991	0.439
LIG_LIR_Nem_3	54	58	PF02991	0.361
LIG_NRBOX	261	267	PF00104	0.514
LIG_REV1ctd_RIR_1	203	209	PF16727	0.473
LIG_SH2_CRK	108	112	PF00017	0.526
LIG_SH2_CRK	330	334	PF00017	0.436
LIG_SH2_CRK	43	47	PF00017	0.313
LIG_SH2_NCK_1	330	334	PF00017	0.430
LIG_SH2_PTP2	263	266	PF00017	0.507
LIG_SH2_SRC	357	360	PF00017	0.514
LIG_SH2_STAP1	518	522	PF00017	0.515
LIG_SH2_STAT5	246	249	PF00017	0.446
LIG_SH2_STAT5	263	266	PF00017	0.443
LIG_SH2_STAT5	316	319	PF00017	0.481
LIG_SH2_STAT5	357	360	PF00017	0.514
LIG_SH3_3	244	250	PF00018	0.441
LIG_SH3_3	288	294	PF00018	0.612
LIG_SH3_3	78	84	PF00018	0.554
LIG_SUMO_SIM_anti_2	421	426	PF11976	0.394
LIG_SUMO_SIM_par_1	263	269	PF11976	0.535
LIG_SUMO_SIM_par_1	420	426	PF11976	0.285
LIG_SUMO_SIM_par_1	586	591	PF11976	0.477
LIG_TRAF2_1	224	227	PF00917	0.451
LIG_TYR_ITSM	39	46	PF00017	0.366
LIG_WRC_WIRS_1	396	401	PF05994	0.454
MOD_CDC14_SPxK_1	77	80	PF00782	0.538
MOD_CDK_SPxK_1	74	80	PF00069	0.509
MOD_CK1_1	138	144	PF00069	0.515
MOD_CK1_1	174	180	PF00069	0.434
MOD_CK1_1	275	281	PF00069	0.703
MOD_CK1_1	282	288	PF00069	0.660
MOD_CK1_1	289	295	PF00069	0.597
MOD_CK1_1	301	307	PF00069	0.547
MOD_CK1_1	96	102	PF00069	0.310
MOD_CK2_1	158	164	PF00069	0.560
MOD_CK2_1	209	215	PF00069	0.556
MOD_CK2_1	220	226	PF00069	0.479
MOD_CK2_1	227	233	PF00069	0.393
MOD_CK2_1	395	401	PF00069	0.474
MOD_CK2_1	412	418	PF00069	0.379
MOD_CK2_1	423	429	PF00069	0.309
MOD_CK2_1	447	453	PF00069	0.514
MOD_CK2_1	57	63	PF00069	0.462
MOD_Cter_Amidation	254	257	PF01082	0.295
MOD_DYRK1A_RPxSP_1	80	84	PF00069	0.504
MOD_GlcNHglycan	140	143	PF01048	0.282
MOD_GlcNHglycan	21	24	PF01048	0.706
MOD_GlcNHglycan	278	281	PF01048	0.529
MOD_GlcNHglycan	29	33	PF01048	0.611
MOD_GlcNHglycan	425	428	PF01048	0.312
MOD_GlcNHglycan	490	493	PF01048	0.293
MOD_GlcNHglycan	555	558	PF01048	0.498
MOD_GSK3_1	131	138	PF00069	0.506
MOD_GSK3_1	167	174	PF00069	0.448
MOD_GSK3_1	272	279	PF00069	0.643
MOD_GSK3_1	282	289	PF00069	0.642
MOD_GSK3_1	294	301	PF00069	0.661
MOD_GSK3_1	339	346	PF00069	0.496
MOD_GSK3_1	385	392	PF00069	0.553
MOD_GSK3_1	395	402	PF00069	0.438
MOD_GSK3_1	53	60	PF00069	0.443
MOD_GSK3_1	564	571	PF00069	0.503
MOD_GSK3_1	93	100	PF00069	0.421
MOD_N-GLC_1	301	306	PF02516	0.661
MOD_N-GLC_1	473	478	PF02516	0.230
MOD_NEK2_1	131	136	PF00069	0.485
MOD_NEK2_1	167	172	PF00069	0.516
MOD_NEK2_1	266	271	PF00069	0.423
MOD_NEK2_1	399	404	PF00069	0.494
MOD_NEK2_1	480	485	PF00069	0.455
MOD_NEK2_1	494	499	PF00069	0.455
MOD_NEK2_1	500	505	PF00069	0.321
MOD_PIKK_1	372	378	PF00454	0.453
MOD_PK_1	539	545	PF00069	0.311
MOD_PKA_1	276	282	PF00069	0.721
MOD_PKA_2	219	225	PF00069	0.526
MOD_PKA_2	275	281	PF00069	0.708
MOD_PKA_2	38	44	PF00069	0.468
MOD_PKA_2	412	418	PF00069	0.371
MOD_PKA_2	494	500	PF00069	0.514
MOD_Plk_1	159	165	PF00069	0.468
MOD_Plk_1	53	59	PF00069	0.501
MOD_Plk_1	93	99	PF00069	0.357
MOD_Plk_2-3	227	233	PF00069	0.489
MOD_Plk_2-3	59	65	PF00069	0.558
MOD_Plk_4	352	358	PF00069	0.514
MOD_Plk_4	395	401	PF00069	0.469
MOD_Plk_4	420	426	PF00069	0.311
MOD_Plk_4	475	481	PF00069	0.430
MOD_Plk_4	93	99	PF00069	0.357
MOD_ProDKin_1	221	227	PF00069	0.443
MOD_ProDKin_1	473	479	PF00069	0.430
MOD_ProDKin_1	74	80	PF00069	0.509
MOD_SUMO_rev_2	519	528	PF00179	0.533
TRG_DiLeu_BaLyEn_6	247	252	PF01217	0.495
TRG_DiLeu_BaLyEn_6	496	501	PF01217	0.455
TRG_DiLeu_BaLyEn_6	547	552	PF01217	0.467
TRG_ENDOCYTIC_2	108	111	PF00928	0.526
TRG_ENDOCYTIC_2	263	266	PF00928	0.443
TRG_ENDOCYTIC_2	43	46	PF00928	0.322
TRG_ER_diArg_1	127	129	PF00400	0.479
TRG_ER_diArg_1	187	189	PF00400	0.468
TRG_ER_diArg_1	410	413	PF00400	0.404
TRG_ER_diArg_1	431	433	PF00400	0.434
TRG_ER_diArg_1	548	550	PF00400	0.460
TRG_ER_diArg_1	592	594	PF00400	0.509
TRG_NES_CRM1_1	418	434	PF08389	0.428
TRG_NES_CRM1_1	64	79	PF08389	0.306
TRG_Pf-PMV_PEXEL_1	128	132	PF00026	0.305
TRG_Pf-PMV_PEXEL_1	189	193	PF00026	0.249
TRG_Pf-PMV_PEXEL_1	414	418	PF00026	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAP5	Leptomonas seymouri	25%	80%
A0A0N1IH77	Leptomonas seymouri	54%	100%
A0A0S4J7C9	Bodo saltans	25%	90%
A0A0S4JEF0	Bodo saltans	26%	100%
A0A0S4JH38	Bodo saltans	34%	97%
A0A0S4KQK8	Bodo saltans	28%	91%
A0A1X0NZP1	Trypanosomatidae	24%	100%
A0A1X0P0Y5	Trypanosomatidae	36%	100%
A0A1X0P6A0	Trypanosomatidae	25%	89%
A0A3Q8IDL7	Leishmania donovani	23%	82%
A0A3Q8III2	Leishmania donovani	27%	82%
A0A3S7X261	Leishmania donovani	90%	96%
A0A422N064	Trypanosoma rangeli	42%	100%
A0A422P136	Trypanosoma rangeli	25%	91%
A4HH59	Leishmania braziliensis	78%	100%
A4HNF6	Leishmania braziliensis	26%	83%
A4HYN9	Leishmania infantum	23%	82%
A4I479	Leishmania infantum	90%	96%
A4IC28	Leishmania infantum	27%	82%
A4Q9E8	Mus musculus	27%	72%
A4Q9F6	Mus musculus	25%	74%
A6NNM8	Homo sapiens	25%	73%
A8CVX7	Danio rerio	28%	76%
A8X9V4	Caenorhabditis briggsae	24%	100%
B6DTF7	Bodo saltans	23%	100%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	87%
C9ZKP9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
C9ZPR8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
C9ZYH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	91%
D0A044	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	100%
E9ADM8	Leishmania major	91%	100%
E9AFW7	Leishmania major	27%	82%
E9AID0	Leishmania braziliensis	24%	100%
E9AUL7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
E9AZF8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9B720	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	82%
Q8N841	Homo sapiens	26%	71%
V5ARK1	Trypanosoma cruzi	26%	100%
V5B4U2	Trypanosoma cruzi	42%	100%