FAD-binding PCMH-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein

Species:

Leishmania mexicana

UniProt:

E9AM50_LEIMU

TriTrypDb:

LmxM.08_29.0280

Length:

491

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AM50

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AM50

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	2
GO:0006089	lactate metabolic process	4	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009987	cellular process	1	2
GO:0016054	organic acid catabolic process	4	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0032787	monocarboxylic acid metabolic process	6	2
GO:0043436	oxoacid metabolic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044281	small molecule metabolic process	2	2
GO:0044282	small molecule catabolic process	3	2
GO:0046395	carboxylic acid catabolic process	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0072329	monocarboxylic acid catabolic process	6	2
GO:1901575	organic substance catabolic process	3	2
GO:1901615	organic hydroxy compound metabolic process	3	2
GO:1901616	organic hydroxy compound catabolic process	4	2
GO:1903457	lactate catabolic process	5	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0050660	flavin adenine dinucleotide binding	4	13
GO:0071949	FAD binding	5	13
GO:0097159	organic cyclic compound binding	2	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13
GO:0004457	lactate dehydrogenase activity	4	2
GO:0004458	D-lactate dehydrogenase (cytochrome) activity	5	2
GO:0008720	D-lactate dehydrogenase activity	5	2
GO:0016491	oxidoreductase activity	2	2
GO:0016614	oxidoreductase activity, acting on CH-OH group of donors	3	2
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	2
GO:0016898	oxidoreductase activity, acting on the CH-OH group of donors, cytochrome as acceptor	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	130	132	PF00675	0.415
CLV_NRD_NRD_1	198	200	PF00675	0.465
CLV_NRD_NRD_1	281	283	PF00675	0.166
CLV_NRD_NRD_1	303	305	PF00675	0.312
CLV_NRD_NRD_1	341	343	PF00675	0.317
CLV_NRD_NRD_1	425	427	PF00675	0.255
CLV_NRD_NRD_1	43	45	PF00675	0.338
CLV_PCSK_KEX2_1	303	305	PF00082	0.377
CLV_PCSK_KEX2_1	341	343	PF00082	0.297
CLV_PCSK_KEX2_1	414	416	PF00082	0.366
CLV_PCSK_KEX2_1	425	427	PF00082	0.311
CLV_PCSK_PC1ET2_1	414	416	PF00082	0.318
CLV_PCSK_SKI1_1	131	135	PF00082	0.358
CLV_PCSK_SKI1_1	183	187	PF00082	0.297
CLV_PCSK_SKI1_1	446	450	PF00082	0.419
CLV_PCSK_SKI1_1	464	468	PF00082	0.262
DEG_SIAH_1	58	66	PF03145	0.297
DOC_CKS1_1	184	189	PF01111	0.297
DOC_CKS1_1	84	89	PF01111	0.297
DOC_MAPK_gen_1	199	207	PF00069	0.415
DOC_MAPK_gen_1	282	288	PF00069	0.174
DOC_MAPK_MEF2A_6	176	184	PF00069	0.297
DOC_USP7_MATH_1	148	152	PF00917	0.320
DOC_USP7_MATH_1	340	344	PF00917	0.366
DOC_USP7_UBL2_3	137	141	PF12436	0.311
DOC_USP7_UBL2_3	319	323	PF12436	0.408
DOC_USP7_UBL2_3	45	49	PF12436	0.355
DOC_WW_Pin1_4	183	188	PF00397	0.309
DOC_WW_Pin1_4	83	88	PF00397	0.297
LIG_14-3-3_CanoR_1	131	136	PF00244	0.335
LIG_14-3-3_CanoR_1	199	207	PF00244	0.425
LIG_14-3-3_CanoR_1	341	349	PF00244	0.411
LIG_14-3-3_CanoR_1	359	369	PF00244	0.176
LIG_14-3-3_CanoR_1	64	74	PF00244	0.257
LIG_Actin_WH2_2	123	138	PF00022	0.250
LIG_APCC_ABBA_1	404	409	PF00400	0.250
LIG_APCC_ABBAyCdc20_2	303	309	PF00400	0.212
LIG_BIR_II_1	1	5	PF00653	0.477
LIG_BRCT_BRCA1_1	342	346	PF00533	0.477
LIG_FHA_1	107	113	PF00498	0.297
LIG_FHA_1	192	198	PF00498	0.387
LIG_FHA_1	215	221	PF00498	0.320
LIG_FHA_1	36	42	PF00498	0.324
LIG_FHA_1	66	72	PF00498	0.250
LIG_FHA_2	111	117	PF00498	0.335
LIG_FHA_2	172	178	PF00498	0.299
LIG_FHA_2	334	340	PF00498	0.212
LIG_FHA_2	377	383	PF00498	0.341
LIG_FHA_2	90	96	PF00498	0.332
LIG_LIR_Gen_1	343	351	PF02991	0.250
LIG_LIR_Gen_1	398	407	PF02991	0.372
LIG_LIR_Nem_3	223	229	PF02991	0.409
LIG_LIR_Nem_3	309	313	PF02991	0.297
LIG_LIR_Nem_3	398	404	PF02991	0.382
LIG_LIR_Nem_3	6	12	PF02991	0.365
LIG_MLH1_MIPbox_1	342	346	PF16413	0.296
LIG_SH2_CRK	310	314	PF00017	0.250
LIG_SH2_CRK	81	85	PF00017	0.250
LIG_SH2_STAT3	479	482	PF00017	0.361
LIG_SH2_STAT5	211	214	PF00017	0.317
LIG_SH2_STAT5	327	330	PF00017	0.390
LIG_SH2_STAT5	345	348	PF00017	0.390
LIG_SH2_STAT5	350	353	PF00017	0.289
LIG_SH3_1	81	87	PF00018	0.250
LIG_SH3_3	142	148	PF00018	0.250
LIG_SH3_3	181	187	PF00018	0.319
LIG_SH3_3	362	368	PF00018	0.332
LIG_SH3_3	481	487	PF00018	0.403
LIG_SH3_3	81	87	PF00018	0.297
LIG_TRAF2_1	3	6	PF00917	0.562
LIG_TRAF2_1	378	381	PF00917	0.311
LIG_TRFH_1	226	230	PF08558	0.394
LIG_UBA3_1	228	233	PF00899	0.395
MOD_CDK_SPK_2	183	188	PF00069	0.288
MOD_CDK_SPK_2	83	88	PF00069	0.332
MOD_CK1_1	214	220	PF00069	0.321
MOD_CK1_1	298	304	PF00069	0.198
MOD_CK1_1	35	41	PF00069	0.413
MOD_CK2_1	171	177	PF00069	0.297
MOD_CK2_1	333	339	PF00069	0.305
MOD_CK2_1	89	95	PF00069	0.297
MOD_Cter_Amidation	301	304	PF01082	0.221
MOD_DYRK1A_RPxSP_1	183	187	PF00069	0.332
MOD_GlcNHglycan	1	4	PF01048	0.556
MOD_GlcNHglycan	162	165	PF01048	0.300
MOD_GlcNHglycan	201	204	PF01048	0.444
MOD_GlcNHglycan	235	238	PF01048	0.384
MOD_GlcNHglycan	346	349	PF01048	0.472
MOD_GlcNHglycan	434	437	PF01048	0.300
MOD_GSK3_1	10	17	PF00069	0.495
MOD_GSK3_1	106	113	PF00069	0.407
MOD_GSK3_1	162	169	PF00069	0.323
MOD_GSK3_1	340	347	PF00069	0.375
MOD_GSK3_1	35	42	PF00069	0.411
MOD_GSK3_1	446	453	PF00069	0.166
MOD_GSK3_1	45	52	PF00069	0.360
MOD_N-GLC_1	65	70	PF02516	0.250
MOD_N-GLC_2	121	123	PF02516	0.332
MOD_N-GLC_2	285	287	PF02516	0.166
MOD_NEK2_1	119	124	PF00069	0.250
MOD_NEK2_1	159	164	PF00069	0.348
MOD_NEK2_1	205	210	PF00069	0.372
MOD_NEK2_1	241	246	PF00069	0.385
MOD_NEK2_1	328	333	PF00069	0.193
MOD_NEK2_1	430	435	PF00069	0.390
MOD_NEK2_2	166	171	PF00069	0.297
MOD_NEK2_2	340	345	PF00069	0.406
MOD_PIKK_1	205	211	PF00454	0.368
MOD_PKA_1	131	137	PF00069	0.311
MOD_PKA_1	199	205	PF00069	0.436
MOD_PKA_2	14	20	PF00069	0.342
MOD_PKA_2	198	204	PF00069	0.430
MOD_PKA_2	281	287	PF00069	0.230
MOD_PKA_2	340	346	PF00069	0.411
MOD_Plk_1	119	125	PF00069	0.250
MOD_Plk_1	205	211	PF00069	0.365
MOD_Plk_4	260	266	PF00069	0.316
MOD_Plk_4	32	38	PF00069	0.487
MOD_Plk_4	400	406	PF00069	0.250
MOD_ProDKin_1	183	189	PF00069	0.309
MOD_ProDKin_1	83	89	PF00069	0.297
MOD_SUMO_rev_2	324	334	PF00179	0.250
MOD_SUMO_rev_2	409	416	PF00179	0.332
TRG_ENDOCYTIC_2	310	313	PF00928	0.390
TRG_ER_diArg_1	424	426	PF00400	0.255
TRG_PTS1	488	491	PF00515	0.429

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Q0	Leptomonas seymouri	27%	95%
A0A0N1PE88	Leptomonas seymouri	61%	99%
A0A0N1PEY1	Leptomonas seymouri	24%	79%
A0A0S4IT80	Bodo saltans	25%	100%
A0A0S4JD99	Bodo saltans	56%	100%
A0A0S4JV88	Bodo saltans	25%	77%
A0A1X0NXE7	Trypanosomatidae	25%	95%
A0A1X0P026	Trypanosomatidae	61%	100%
A0A1X0P2J0	Trypanosomatidae	24%	80%
A0A3Q8IDH3	Leishmania donovani	92%	100%
A0A3Q8IDH5	Leishmania donovani	61%	100%
A0A3Q8IEJ5	Leishmania donovani	26%	95%
A0A3Q8IRN9	Leishmania donovani	23%	79%
A0A422N649	Trypanosoma rangeli	25%	80%
A0A422N8Z9	Trypanosoma rangeli	26%	95%
A1L258	Danio rerio	25%	92%
A4HCV1	Leishmania braziliensis	26%	100%
A4HFX9	Leishmania braziliensis	61%	100%
A4HHV7	Leishmania braziliensis	24%	100%
A4I0D2	Leishmania infantum	26%	95%
A4I309	Leishmania infantum	62%	100%
A4I481	Leishmania infantum	92%	100%
A4I507	Leishmania infantum	23%	79%
A4VGK4	Pseudomonas stutzeri (strain A1501)	26%	100%
B8B7X6	Oryza sativa subsp. indica	26%	88%
C9ZQF7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	80%
D4MUV9	Anaerostipes hadrus	30%	100%
E9ADI2	Leishmania major	61%	100%
E9ADN0	Leishmania major	91%	100%
E9AW96	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	95%
E9AZA6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	61%	100%
E9B0D7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	79%
F1QXM5	Danio rerio	34%	99%
H6LBS1	Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1)	29%	100%
O23240	Arabidopsis thaliana	24%	88%
O29853	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	31%	100%
O45218	Caenorhabditis elegans	23%	82%
O96759	Dictyostelium discoideum	25%	80%
O97157	Trypanosoma brucei brucei	24%	80%
P0AEP9	Escherichia coli (strain K12)	30%	98%
P0AEQ0	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	30%	98%
P0DV35	Xanthomonas citri pv. viticola (strain LMG 965 / NCPPB 2475 / ICMP 3867 / CFBP 7660)	25%	100%
P32891	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	84%
P39976	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	99%
P46681	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	93%
P84850	Rattus norvegicus	25%	92%
P94535	Bacillus subtilis (strain 168)	30%	100%
P9WIT0	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	29%	100%
P9WIT1	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	29%	100%
Q12627	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	32%	85%
Q1JPD3	Bos taurus	27%	90%
Q46911	Escherichia coli (strain K12)	24%	100%
Q4QB82	Leishmania major	25%	100%
Q7TNG8	Mus musculus	34%	100%
Q7XI14	Oryza sativa subsp. japonica	26%	88%
Q7YWB6	Leishmania major	24%	100%
Q86WU2	Homo sapiens	33%	97%
Q8CIM3	Mus musculus	26%	92%
Q8N465	Homo sapiens	25%	94%
Q8X7S0	Escherichia coli O157:H7	24%	100%
Q94AX4	Arabidopsis thaliana	35%	87%
Q9C1X2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	93%
Q9V778	Drosophila melanogaster	24%	78%
V5BCB2	Trypanosoma cruzi	25%	80%
V5D7M3	Trypanosoma cruzi	26%	97%