Putative flagellar radial spoke protein-like

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

flagellar radial spoke protein-like, putative

Species:

Leishmania mexicana

UniProt:

E9AM12_LEIMU

TriTrypDb:

LmxM.08_29.0660

Length:

588

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0001534	radial spoke	2	12
GO:0005929	cilium	4	12
GO:0031514	motile cilium	5	12
GO:0032991	protein-containing complex	1	12
GO:0042995	cell projection	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0120025	plasma membrane bounded cell projection	3	12
GO:0005930	axoneme	2	1

Expansion

Sequence features

E9AM12

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AM12

Function

Biological processes
Term	Name	Level	Count
GO:0003341	cilium movement	4	12
GO:0006996	organelle organization	4	12
GO:0007017	microtubule-based process	2	12
GO:0007018	microtubule-based movement	3	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0030030	cell projection organization	4	11
GO:0030031	cell projection assembly	5	11
GO:0044782	cilium organization	5	11
GO:0060271	cilium assembly	6	11
GO:0060294	cilium movement involved in cell motility	5	12
GO:0070925	organelle assembly	5	11
GO:0071840	cellular component organization or biogenesis	2	12
GO:0120031	plasma membrane bounded cell projection assembly	6	11
GO:0120036	plasma membrane bounded cell projection organization	5	11
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0001578	microtubule bundle formation	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0035082	axoneme assembly	5	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	239	241	PF00675	0.316
CLV_NRD_NRD_1	480	482	PF00675	0.243
CLV_NRD_NRD_1	94	96	PF00675	0.266
CLV_PCSK_KEX2_1	239	241	PF00082	0.317
CLV_PCSK_KEX2_1	94	96	PF00082	0.266
DEG_COP1_1	332	342	PF00400	0.556
DOC_ANK_TNKS_1	574	581	PF00023	0.656
DOC_CDC14_PxL_1	361	369	PF14671	0.463
DOC_CYCLIN_yCln2_LP_2	461	467	PF00134	0.556
DOC_PP1_RVXF_1	495	502	PF00149	0.538
DOC_PP2B_LxvP_1	146	149	PF13499	0.538
DOC_PP2B_LxvP_1	296	299	PF13499	0.528
DOC_PP2B_LxvP_1	461	464	PF13499	0.556
DOC_PP2B_LxvP_1	540	543	PF13499	0.538
DOC_PP2B_LxvP_1	87	90	PF13499	0.452
DOC_USP7_MATH_1	242	246	PF00917	0.533
DOC_USP7_MATH_1	262	266	PF00917	0.321
DOC_USP7_MATH_1	438	442	PF00917	0.556
DOC_USP7_MATH_1	468	472	PF00917	0.498
DOC_USP7_MATH_1	515	519	PF00917	0.513
DOC_USP7_MATH_1	70	74	PF00917	0.515
DOC_USP7_UBL2_3	389	393	PF12436	0.493
DOC_WW_Pin1_4	275	280	PF00397	0.477
DOC_WW_Pin1_4	441	446	PF00397	0.493
LIG_14-3-3_CanoR_1	10	18	PF00244	0.556
LIG_14-3-3_CanoR_1	320	329	PF00244	0.452
LIG_14-3-3_CanoR_1	414	419	PF00244	0.502
LIG_14-3-3_CanoR_1	514	520	PF00244	0.535
LIG_BIR_II_1	1	5	PF00653	0.590
LIG_BIR_III_4	35	39	PF00653	0.493
LIG_eIF4E_1	391	397	PF01652	0.452
LIG_EVH1_2	541	545	PF00568	0.415
LIG_FHA_1	17	23	PF00498	0.513
LIG_FHA_1	291	297	PF00498	0.404
LIG_FHA_1	415	421	PF00498	0.552
LIG_FHA_1	504	510	PF00498	0.474
LIG_FHA_2	262	268	PF00498	0.526
LIG_FHA_2	390	396	PF00498	0.493
LIG_FHA_2	55	61	PF00498	0.353
LIG_FHA_2	560	566	PF00498	0.452
LIG_LIR_Apic_2	566	572	PF02991	0.547
LIG_LIR_Apic_2	57	61	PF02991	0.353
LIG_LIR_Gen_1	269	279	PF02991	0.464
LIG_LIR_Gen_1	518	524	PF02991	0.454
LIG_LIR_Nem_3	152	157	PF02991	0.485
LIG_LIR_Nem_3	171	177	PF02991	0.333
LIG_LIR_Nem_3	181	186	PF02991	0.435
LIG_LIR_Nem_3	269	274	PF02991	0.493
LIG_MYND_1	365	369	PF01753	0.463
LIG_MYND_1	538	542	PF01753	0.433
LIG_PDZ_Class_3	583	588	PF00595	0.727
LIG_Pex14_1	387	391	PF04695	0.452
LIG_PTAP_UEV_1	243	248	PF05743	0.493
LIG_RPA_C_Fungi	315	327	PF08784	0.425
LIG_SH2_CRK	154	158	PF00017	0.425
LIG_SH2_CRK	361	365	PF00017	0.320
LIG_SH2_CRK	536	540	PF00017	0.425
LIG_SH2_CRK	569	573	PF00017	0.556
LIG_SH2_GRB2like	140	143	PF00017	0.449
LIG_SH2_NCK_1	569	573	PF00017	0.556
LIG_SH2_SRC	536	539	PF00017	0.425
LIG_SH2_SRC	548	551	PF00017	0.425
LIG_SH2_SRC	569	572	PF00017	0.572
LIG_SH2_SRC	58	61	PF00017	0.339
LIG_SH2_STAP1	183	187	PF00017	0.340
LIG_SH2_STAT5	140	143	PF00017	0.449
LIG_SH2_STAT5	186	189	PF00017	0.304
LIG_SH2_STAT5	313	316	PF00017	0.304
LIG_SH2_STAT5	391	394	PF00017	0.362
LIG_SH2_STAT5	494	497	PF00017	0.304
LIG_SH2_STAT5	548	551	PF00017	0.449
LIG_SH3_1	535	541	PF00018	0.425
LIG_SH3_1	568	574	PF00018	0.665
LIG_SH3_2	89	94	PF14604	0.304
LIG_SH3_3	241	247	PF00018	0.449
LIG_SH3_3	360	366	PF00018	0.313
LIG_SH3_3	440	446	PF00018	0.432
LIG_SH3_3	535	541	PF00018	0.306
LIG_SH3_3	546	552	PF00018	0.309
LIG_SH3_3	568	574	PF00018	0.545
LIG_SH3_3	86	92	PF00018	0.309
LIG_TRAF2_1	247	250	PF00917	0.425
LIG_TRAF2_1	373	376	PF00917	0.318
LIG_TRAF2_1	419	422	PF00917	0.392
LIG_WRC_WIRS_1	330	335	PF05994	0.449
LIG_WRC_WIRS_1	71	76	PF05994	0.449
LIG_WW_2	538	541	PF00397	0.425
LIG_WW_3	572	576	PF00397	0.688
LIG_WW_3	88	92	PF00397	0.394
MOD_CK1_1	441	447	PF00069	0.461
MOD_CK1_1	457	463	PF00069	0.246
MOD_CK2_1	389	395	PF00069	0.449
MOD_CK2_1	559	565	PF00069	0.425
MOD_GlcNHglycan	11	14	PF01048	0.449
MOD_GlcNHglycan	244	247	PF01048	0.358
MOD_GlcNHglycan	264	267	PF01048	0.288
MOD_GlcNHglycan	322	325	PF01048	0.449
MOD_GlcNHglycan	346	352	PF01048	0.362
MOD_GlcNHglycan	45	48	PF01048	0.400
MOD_GSK3_1	129	136	PF00069	0.421
MOD_GSK3_1	163	170	PF00069	0.460
MOD_GSK3_1	250	257	PF00069	0.297
MOD_GSK3_1	262	269	PF00069	0.300
MOD_GSK3_1	503	510	PF00069	0.398
MOD_GSK3_1	70	77	PF00069	0.390
MOD_N-GLC_1	438	443	PF02516	0.362
MOD_NEK2_1	45	50	PF00069	0.398
MOD_NEK2_1	74	79	PF00069	0.449
MOD_NEK2_2	168	173	PF00069	0.390
MOD_NEK2_2	476	481	PF00069	0.285
MOD_PIKK_1	16	22	PF00454	0.433
MOD_PIKK_1	220	226	PF00454	0.411
MOD_PKA_2	242	248	PF00069	0.425
MOD_PKA_2	290	296	PF00069	0.425
MOD_PKA_2	319	325	PF00069	0.320
MOD_PKA_2	413	419	PF00069	0.390
MOD_PKA_2	61	67	PF00069	0.408
MOD_PKA_2	9	15	PF00069	0.449
MOD_Plk_1	457	463	PF00069	0.427
MOD_Plk_1	559	565	PF00069	0.390
MOD_Plk_4	405	411	PF00069	0.309
MOD_ProDKin_1	275	281	PF00069	0.339
MOD_ProDKin_1	441	447	PF00069	0.362
TRG_DiLeu_BaLyEn_6	152	157	PF01217	0.382
TRG_DiLeu_BaLyEn_6	363	368	PF01217	0.390
TRG_ENDOCYTIC_2	140	143	PF00928	0.456
TRG_ENDOCYTIC_2	154	157	PF00928	0.353
TRG_ENDOCYTIC_2	361	364	PF00928	0.304
TRG_ENDOCYTIC_2	519	522	PF00928	0.322
TRG_ER_diArg_1	239	241	PF00400	0.392
TRG_ER_diArg_1	288	291	PF00400	0.339
TRG_ER_diArg_1	93	95	PF00400	0.325

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8Q1	Leptomonas seymouri	70%	100%
A0A0S4JES8	Bodo saltans	41%	100%
A0A1X0P128	Trypanosomatidae	47%	100%
A0A3Q8IE67	Leishmania donovani	95%	82%
A0A3R7KK84	Trypanosoma rangeli	48%	100%
A4HH73	Leishmania braziliensis	84%	100%
A4I4B9	Leishmania infantum	95%	84%
C9ZL49	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	100%
E9ADR8	Leishmania major	93%	100%
Q01656	Chlamydomonas reinhardtii	24%	100%
Q8BYM7	Mus musculus	25%	82%
Q8CDR2	Mus musculus	29%	83%
Q9H0K4	Homo sapiens	28%	82%
V5B4W9	Trypanosoma cruzi	47%	100%