HotDog ACOT-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Acyl-coenzyme A thioesterase, putative

Species:

Leishmania mexicana

UniProt:

E9ALQ4_LEIMU

TriTrypDb:

LmxM.08_29.1690

Length:

398

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ALQ4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ALQ4

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006163	purine nucleotide metabolic process	5	1
GO:0006629	lipid metabolic process	3	1
GO:0006631	fatty acid metabolic process	4	1
GO:0006637	acyl-CoA metabolic process	4	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006790	sulfur compound metabolic process	3	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009150	purine ribonucleotide metabolic process	6	1
GO:0009259	ribonucleotide metabolic process	5	1
GO:0009987	cellular process	1	1
GO:0019637	organophosphate metabolic process	3	1
GO:0019693	ribose phosphate metabolic process	4	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0032787	monocarboxylic acid metabolic process	6	1
GO:0033865	nucleoside bisphosphate metabolic process	5	1
GO:0033875	ribonucleoside bisphosphate metabolic process	6	1
GO:0034032	purine nucleoside bisphosphate metabolic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0035383	thioester metabolic process	3	1
GO:0043436	oxoacid metabolic process	4	1
GO:0043603	amide metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0044281	small molecule metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0072521	purine-containing compound metabolic process	4	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0016787	hydrolase activity	2	13
GO:0016788	hydrolase activity, acting on ester bonds	3	13
GO:0016790	thiolester hydrolase activity	4	13
GO:0000062	fatty-acyl-CoA binding	4	1
GO:0000166	nucleotide binding	3	4
GO:0005488	binding	1	4
GO:0008289	lipid binding	2	1
GO:0016289	CoA hydrolase activity	5	1
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0033218	amide binding	2	1
GO:0036042	long-chain fatty acyl-CoA binding	5	1
GO:0036094	small molecule binding	2	4
GO:0047617	acyl-CoA hydrolase activity	6	1
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:0120227	acyl-CoA binding	3	1
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4
GO:1901567	fatty acid derivative binding	3	1
GO:1901681	sulfur compound binding	2	1
GO:0005524	ATP binding	5	3
GO:0035639	purine ribonucleoside triphosphate binding	4	3
GO:0043167	ion binding	2	3
GO:0043168	anion binding	3	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	237	241	PF00656	0.220
CLV_NRD_NRD_1	196	198	PF00675	0.523
CLV_NRD_NRD_1	3	5	PF00675	0.464
CLV_NRD_NRD_1	325	327	PF00675	0.432
CLV_NRD_NRD_1	347	349	PF00675	0.524
CLV_NRD_NRD_1	382	384	PF00675	0.553
CLV_PCSK_FUR_1	210	214	PF00082	0.591
CLV_PCSK_KEX2_1	212	214	PF00082	0.585
CLV_PCSK_KEX2_1	3	5	PF00082	0.464
CLV_PCSK_KEX2_1	382	384	PF00082	0.509
CLV_PCSK_PC1ET2_1	212	214	PF00082	0.574
CLV_PCSK_SKI1_1	100	104	PF00082	0.448
CLV_PCSK_SKI1_1	171	175	PF00082	0.429
CLV_PCSK_SKI1_1	213	217	PF00082	0.458
CLV_PCSK_SKI1_1	243	247	PF00082	0.443
CLV_PCSK_SKI1_1	274	278	PF00082	0.375
CLV_PCSK_SKI1_1	280	284	PF00082	0.310
DEG_MDM2_SWIB_1	297	304	PF02201	0.344
DEG_Nend_Nbox_1	1	3	PF02207	0.493
DOC_CKS1_1	113	118	PF01111	0.230
DOC_CYCLIN_yCln2_LP_2	181	187	PF00134	0.484
DOC_MAPK_gen_1	171	181	PF00069	0.404
DOC_MAPK_gen_1	348	356	PF00069	0.454
DOC_MAPK_HePTP_8	346	358	PF00069	0.496
DOC_MAPK_MEF2A_6	312	320	PF00069	0.285
DOC_MAPK_MEF2A_6	348	356	PF00069	0.441
DOC_MAPK_RevD_3	185	198	PF00069	0.414
DOC_PP1_RVXF_1	241	247	PF00149	0.507
DOC_PP4_FxxP_1	113	116	PF00568	0.372
DOC_PP4_FxxP_1	292	295	PF00568	0.272
DOC_USP7_MATH_1	156	160	PF00917	0.423
DOC_USP7_MATH_1	83	87	PF00917	0.471
DOC_USP7_MATH_1	9	13	PF00917	0.619
DOC_USP7_UBL2_3	191	195	PF12436	0.391
DOC_USP7_UBL2_3	323	327	PF12436	0.390
DOC_WW_Pin1_4	112	117	PF00397	0.425
DOC_WW_Pin1_4	144	149	PF00397	0.420
DOC_WW_Pin1_4	164	169	PF00397	0.413
DOC_WW_Pin1_4	23	28	PF00397	0.613
DOC_WW_Pin1_4	61	66	PF00397	0.520
LIG_14-3-3_CanoR_1	100	108	PF00244	0.429
LIG_14-3-3_CanoR_1	210	216	PF00244	0.558
LIG_14-3-3_CanoR_1	312	317	PF00244	0.292
LIG_14-3-3_CanoR_1	335	339	PF00244	0.339
LIG_14-3-3_CanoR_1	375	381	PF00244	0.430
LIG_14-3-3_CanoR_1	84	90	PF00244	0.448
LIG_Actin_WH2_2	156	173	PF00022	0.366
LIG_AP2alpha_2	183	185	PF02296	0.400
LIG_BRCT_BRCA1_1	11	15	PF00533	0.718
LIG_BRCT_BRCA1_1	288	292	PF00533	0.344
LIG_CaM_IQ_9	196	212	PF13499	0.553
LIG_FHA_1	101	107	PF00498	0.373
LIG_FHA_1	113	119	PF00498	0.410
LIG_FHA_1	150	156	PF00498	0.407
LIG_FHA_1	165	171	PF00498	0.446
LIG_FHA_1	215	221	PF00498	0.581
LIG_FHA_1	297	303	PF00498	0.320
LIG_FHA_1	313	319	PF00498	0.278
LIG_FHA_1	327	333	PF00498	0.399
LIG_FHA_1	34	40	PF00498	0.465
LIG_FHA_1	349	355	PF00498	0.453
LIG_FHA_1	9	15	PF00498	0.537
LIG_FHA_2	148	154	PF00498	0.453
LIG_FHA_2	214	220	PF00498	0.442
LIG_FHA_2	235	241	PF00498	0.463
LIG_FHA_2	312	318	PF00498	0.306
LIG_FHA_2	360	366	PF00498	0.425
LIG_LIR_Apic_2	111	116	PF02991	0.422
LIG_LIR_Apic_2	289	295	PF02991	0.287
LIG_LIR_Gen_1	159	168	PF02991	0.350
LIG_LIR_Gen_1	298	309	PF02991	0.320
LIG_LIR_Gen_1	337	346	PF02991	0.328
LIG_LIR_Gen_1	54	59	PF02991	0.419
LIG_LIR_Nem_3	159	164	PF02991	0.385
LIG_LIR_Nem_3	298	304	PF02991	0.330
LIG_LIR_Nem_3	337	341	PF02991	0.325
LIG_LIR_Nem_3	54	58	PF02991	0.427
LIG_PCNA_yPIPBox_3	363	375	PF02747	0.465
LIG_PDZ_Class_1	393	398	PF00595	0.636
LIG_Pex14_2	297	301	PF04695	0.320
LIG_PTB_Apo_2	107	114	PF02174	0.401
LIG_SH2_GRB2like	249	252	PF00017	0.238
LIG_SH2_PTP2	193	196	PF00017	0.416
LIG_SH2_STAT5	101	104	PF00017	0.476
LIG_SH2_STAT5	140	143	PF00017	0.407
LIG_SH2_STAT5	193	196	PF00017	0.430
LIG_SH2_STAT5	310	313	PF00017	0.309
LIG_SH3_3	287	293	PF00018	0.371
LIG_SUMO_SIM_anti_2	317	322	PF11976	0.378
LIG_SUMO_SIM_par_1	162	167	PF11976	0.384
LIG_SUMO_SIM_par_1	25	32	PF11976	0.412
LIG_TRAF2_1	216	219	PF00917	0.551
LIG_TRAF2_1	361	364	PF00917	0.449
MOD_CDK_SPxxK_3	144	151	PF00069	0.523
MOD_CDK_SPxxK_3	164	171	PF00069	0.484
MOD_CDK_SPxxK_3	61	68	PF00069	0.543
MOD_CK1_1	147	153	PF00069	0.420
MOD_CK1_1	159	165	PF00069	0.308
MOD_CK1_1	22	28	PF00069	0.572
MOD_CK1_1	61	67	PF00069	0.571
MOD_CK2_1	147	153	PF00069	0.473
MOD_CK2_1	174	180	PF00069	0.256
MOD_CK2_1	213	219	PF00069	0.496
MOD_CK2_1	311	317	PF00069	0.301
MOD_CK2_1	359	365	PF00069	0.412
MOD_Cter_Amidation	346	349	PF01082	0.527
MOD_GlcNHglycan	16	19	PF01048	0.561
MOD_GlcNHglycan	176	179	PF01048	0.244
MOD_GSK3_1	102	109	PF00069	0.379
MOD_GSK3_1	147	154	PF00069	0.426
MOD_GSK3_1	19	26	PF00069	0.516
MOD_GSK3_1	296	303	PF00069	0.309
MOD_GSK3_1	326	333	PF00069	0.403
MOD_GSK3_1	386	393	PF00069	0.615
MOD_LATS_1	231	237	PF00433	0.496
MOD_LATS_1	98	104	PF00433	0.469
MOD_N-GLC_1	234	239	PF02516	0.482
MOD_N-GLC_1	252	257	PF02516	0.301
MOD_N-GLC_1	312	317	PF02516	0.320
MOD_N-GLC_2	390	392	PF02516	0.588
MOD_NEK2_1	102	107	PF00069	0.381
MOD_NEK2_1	14	19	PF00069	0.544
MOD_NEK2_1	211	216	PF00069	0.558
MOD_NEK2_1	245	250	PF00069	0.521
MOD_NEK2_1	304	309	PF00069	0.414
MOD_NEK2_2	156	161	PF00069	0.423
MOD_PIKK_1	359	365	PF00454	0.463
MOD_PIKK_1	83	89	PF00454	0.436
MOD_PKA_1	204	210	PF00069	0.566
MOD_PKA_1	326	332	PF00069	0.540
MOD_PKA_1	348	354	PF00069	0.397
MOD_PKA_2	311	317	PF00069	0.292
MOD_PKA_2	334	340	PF00069	0.336
MOD_PKA_2	83	89	PF00069	0.463
MOD_Plk_1	252	258	PF00069	0.301
MOD_Plk_1	304	310	PF00069	0.348
MOD_Plk_1	312	318	PF00069	0.285
MOD_Plk_1	327	333	PF00069	0.432
MOD_Plk_2-3	300	306	PF00069	0.344
MOD_Plk_4	108	114	PF00069	0.416
MOD_Plk_4	156	162	PF00069	0.351
MOD_Plk_4	245	251	PF00069	0.453
MOD_Plk_4	252	258	PF00069	0.338
MOD_Plk_4	304	310	PF00069	0.385
MOD_Plk_4	327	333	PF00069	0.424
MOD_Plk_4	334	340	PF00069	0.292
MOD_ProDKin_1	112	118	PF00069	0.425
MOD_ProDKin_1	144	150	PF00069	0.424
MOD_ProDKin_1	164	170	PF00069	0.416
MOD_ProDKin_1	23	29	PF00069	0.610
MOD_ProDKin_1	61	67	PF00069	0.514
MOD_SUMO_for_1	322	325	PF00179	0.390
MOD_SUMO_rev_2	319	329	PF00179	0.383
TRG_DiLeu_BaEn_3	364	370	PF01217	0.460
TRG_ENDOCYTIC_2	140	143	PF00928	0.425
TRG_ENDOCYTIC_2	193	196	PF00928	0.378
TRG_ER_diArg_1	2	4	PF00400	0.457
TRG_ER_diArg_1	373	376	PF00400	0.402
TRG_Pf-PMV_PEXEL_1	197	201	PF00026	0.594
TRG_Pf-PMV_PEXEL_1	213	218	PF00026	0.477

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2C1	Leptomonas seymouri	33%	88%
A0A0N1PDQ1	Leptomonas seymouri	72%	100%
A0A0S4JR10	Bodo saltans	36%	91%
A0A1X0NZW0	Trypanosomatidae	56%	100%
A0A1X0P0I5	Trypanosomatidae	38%	100%
A0A3S7X2I7	Leishmania donovani	94%	100%
A0A422NEB6	Trypanosoma rangeli	36%	100%
A4HHI1	Leishmania braziliensis	87%	100%
A4I4M9	Leishmania infantum	94%	100%
C9ZLB7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9AE30	Leishmania major	93%	100%
Q8VHQ9	Mus musculus	22%	67%