Putative serine/threonine protein kinase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

5'-AMP-activated protein kinase catalytic subunit alpha, putative

Species:

Leishmania mexicana

UniProt:

E9ALM1_LEIMU

TriTrypDb:

LmxM.08_29.2020

Length:

815

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. None of them appear to be TM.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9ALM1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ALM1

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	11
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:0007165	signal transduction	2	1
GO:0035556	intracellular signal transduction	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004672	protein kinase activity	3	11
GO:0004674	protein serine/threonine kinase activity	4	9
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	444	448	PF00656	0.785
CLV_NRD_NRD_1	329	331	PF00675	0.522
CLV_NRD_NRD_1	353	355	PF00675	0.483
CLV_NRD_NRD_1	504	506	PF00675	0.772
CLV_NRD_NRD_1	534	536	PF00675	0.734
CLV_NRD_NRD_1	615	617	PF00675	0.668
CLV_NRD_NRD_1	671	673	PF00675	0.759
CLV_NRD_NRD_1	742	744	PF00675	0.487
CLV_NRD_NRD_1	811	813	PF00675	0.585
CLV_PCSK_FUR_1	351	355	PF00082	0.539
CLV_PCSK_FUR_1	669	673	PF00082	0.746
CLV_PCSK_FUR_1	740	744	PF00082	0.484
CLV_PCSK_KEX2_1	320	322	PF00082	0.489
CLV_PCSK_KEX2_1	351	353	PF00082	0.484
CLV_PCSK_KEX2_1	504	506	PF00082	0.747
CLV_PCSK_KEX2_1	615	617	PF00082	0.668
CLV_PCSK_KEX2_1	671	673	PF00082	0.759
CLV_PCSK_KEX2_1	742	744	PF00082	0.773
CLV_PCSK_PC1ET2_1	320	322	PF00082	0.480
CLV_PCSK_SKI1_1	137	141	PF00082	0.353
CLV_PCSK_SKI1_1	158	162	PF00082	0.335
DEG_MDM2_SWIB_1	579	586	PF02201	0.494
DEG_SPOP_SBC_1	480	484	PF00917	0.766
DEG_SPOP_SBC_1	752	756	PF00917	0.772
DOC_CKS1_1	103	108	PF01111	0.355
DOC_MAPK_gen_1	158	167	PF00069	0.335
DOC_MAPK_gen_1	306	315	PF00069	0.560
DOC_MAPK_gen_1	327	335	PF00069	0.486
DOC_MAPK_gen_1	781	790	PF00069	0.512
DOC_MAPK_gen_1	79	90	PF00069	0.337
DOC_MAPK_HePTP_8	205	217	PF00069	0.355
DOC_MAPK_MEF2A_6	208	217	PF00069	0.347
DOC_MAPK_MEF2A_6	306	315	PF00069	0.560
DOC_MAPK_MEF2A_6	391	399	PF00069	0.591
DOC_PP1_RVXF_1	172	179	PF00149	0.335
DOC_PP1_RVXF_1	206	212	PF00149	0.355
DOC_PP2B_LxvP_1	277	280	PF13499	0.342
DOC_PP4_FxxP_1	91	94	PF00568	0.335
DOC_USP7_MATH_1	454	458	PF00917	0.789
DOC_USP7_MATH_1	459	463	PF00917	0.712
DOC_USP7_MATH_1	610	614	PF00917	0.647
DOC_USP7_MATH_1	624	628	PF00917	0.545
DOC_USP7_MATH_1	638	642	PF00917	0.495
DOC_USP7_MATH_1	676	680	PF00917	0.698
DOC_USP7_MATH_1	712	716	PF00917	0.729
DOC_USP7_MATH_1	751	755	PF00917	0.731
DOC_USP7_UBL2_3	383	387	PF12436	0.696
DOC_WW_Pin1_4	102	107	PF00397	0.355
DOC_WW_Pin1_4	193	198	PF00397	0.335
DOC_WW_Pin1_4	399	404	PF00397	0.579
DOC_WW_Pin1_4	584	589	PF00397	0.485
DOC_WW_Pin1_4	606	611	PF00397	0.563
DOC_WW_Pin1_4	760	765	PF00397	0.619
LIG_14-3-3_CanoR_1	276	280	PF00244	0.483
LIG_14-3-3_CanoR_1	295	305	PF00244	0.383
LIG_14-3-3_CanoR_1	309	314	PF00244	0.454
LIG_14-3-3_CanoR_1	594	602	PF00244	0.533
LIG_14-3-3_CanoR_1	615	620	PF00244	0.690
LIG_14-3-3_CanoR_1	742	748	PF00244	0.479
LIG_Actin_RPEL_3	147	166	PF02755	0.447
LIG_Actin_WH2_2	59	75	PF00022	0.378
LIG_BIR_II_1	1	5	PF00653	0.728
LIG_BIR_III_3	1	5	PF00653	0.734
LIG_BRCT_BRCA1_1	771	775	PF00533	0.562
LIG_deltaCOP1_diTrp_1	580	589	PF00928	0.298
LIG_FHA_1	597	603	PF00498	0.567
LIG_FHA_1	734	740	PF00498	0.502
LIG_FHA_1	753	759	PF00498	0.747
LIG_FHA_2	103	109	PF00498	0.335
LIG_FHA_2	387	393	PF00498	0.592
LIG_FHA_2	431	437	PF00498	0.705
LIG_FHA_2	539	545	PF00498	0.537
LIG_FHA_2	654	660	PF00498	0.770
LIG_GBD_Chelix_1	798	806	PF00786	0.426
LIG_LIR_Apic_2	196	202	PF02991	0.335
LIG_LIR_Gen_1	107	116	PF02991	0.320
LIG_LIR_Gen_1	120	126	PF02991	0.317
LIG_LIR_Gen_1	570	581	PF02991	0.477
LIG_LIR_Gen_1	58	67	PF02991	0.447
LIG_LIR_Gen_1	598	604	PF02991	0.464
LIG_LIR_Nem_3	120	125	PF02991	0.329
LIG_LIR_Nem_3	46	50	PF02991	0.354
LIG_LIR_Nem_3	570	576	PF02991	0.448
LIG_LIR_Nem_3	58	64	PF02991	0.335
LIG_LIR_Nem_3	580	586	PF02991	0.381
LIG_LIR_Nem_3	587	592	PF02991	0.340
LIG_LIR_Nem_3	598	603	PF02991	0.467
LIG_LIR_Nem_3	772	778	PF02991	0.557
LIG_LIR_Nem_3	789	793	PF02991	0.432
LIG_MAD2	154	162	PF02301	0.447
LIG_MLH1_MIPbox_1	771	775	PF16413	0.562
LIG_NRBOX	223	229	PF00104	0.355
LIG_NRBOX	801	807	PF00104	0.549
LIG_OCRL_FandH_1	774	786	PF00620	0.628
LIG_PCNA_yPIPBox_3	761	774	PF02747	0.588
LIG_Pex14_2	579	583	PF04695	0.442
LIG_REV1ctd_RIR_1	773	781	PF16727	0.621
LIG_SH2_CRK	122	126	PF00017	0.408
LIG_SH2_CRK	225	229	PF00017	0.335
LIG_SH2_CRK	251	255	PF00017	0.355
LIG_SH2_CRK	344	348	PF00017	0.357
LIG_SH2_CRK	573	577	PF00017	0.427
LIG_SH2_NCK_1	100	104	PF00017	0.335
LIG_SH2_NCK_1	199	203	PF00017	0.335
LIG_SH2_NCK_1	251	255	PF00017	0.447
LIG_SH2_PTP2	61	64	PF00017	0.447
LIG_SH2_SRC	115	118	PF00017	0.340
LIG_SH2_SRC	122	125	PF00017	0.342
LIG_SH2_SRC	485	488	PF00017	0.698
LIG_SH2_STAP1	122	126	PF00017	0.355
LIG_SH2_STAP1	298	302	PF00017	0.629
LIG_SH2_STAT3	124	127	PF00017	0.402
LIG_SH2_STAT3	138	141	PF00017	0.249
LIG_SH2_STAT5	110	113	PF00017	0.324
LIG_SH2_STAT5	115	118	PF00017	0.325
LIG_SH2_STAT5	124	127	PF00017	0.335
LIG_SH2_STAT5	138	141	PF00017	0.279
LIG_SH2_STAT5	149	152	PF00017	0.335
LIG_SH2_STAT5	210	213	PF00017	0.328
LIG_SH2_STAT5	251	254	PF00017	0.237
LIG_SH2_STAT5	36	39	PF00017	0.342
LIG_SH2_STAT5	425	428	PF00017	0.380
LIG_SH2_STAT5	540	543	PF00017	0.528
LIG_SH2_STAT5	61	64	PF00017	0.447
LIG_SH2_STAT5	700	703	PF00017	0.746
LIG_SH2_STAT5	774	777	PF00017	0.474
LIG_SH2_STAT5	786	789	PF00017	0.245
LIG_SH3_3	23	29	PF00018	0.709
LIG_SH3_3	267	273	PF00018	0.330
LIG_SH3_3	582	588	PF00018	0.477
LIG_SH3_3	719	725	PF00018	0.524
LIG_SH3_3	762	768	PF00018	0.636
LIG_SUMO_SIM_anti_2	801	808	PF11976	0.524
LIG_SUMO_SIM_anti_2	84	89	PF11976	0.447
LIG_SUMO_SIM_par_1	267	272	PF11976	0.339
LIG_SUMO_SIM_par_1	404	413	PF11976	0.648
LIG_SUMO_SIM_par_1	767	772	PF11976	0.533
LIG_SUMO_SIM_par_1	801	808	PF11976	0.442
LIG_TRAF2_1	541	544	PF00917	0.546
LIG_TYR_ITIM	223	228	PF00017	0.335
LIG_TYR_ITIM	342	347	PF00017	0.354
LIG_UBA3_1	242	250	PF00899	0.335
LIG_WRC_WIRS_1	460	465	PF05994	0.742
LIG_WRC_WIRS_1	576	581	PF05994	0.482
MOD_CDC14_SPxK_1	587	590	PF00782	0.544
MOD_CDK_SPxK_1	584	590	PF00069	0.539
MOD_CK1_1	104	110	PF00069	0.378
MOD_CK1_1	181	187	PF00069	0.471
MOD_CK1_1	193	199	PF00069	0.185
MOD_CK1_1	301	307	PF00069	0.569
MOD_CK1_1	430	436	PF00069	0.643
MOD_CK1_1	441	447	PF00069	0.620
MOD_CK1_1	479	485	PF00069	0.653
MOD_CK1_1	606	612	PF00069	0.644
MOD_CK1_1	620	626	PF00069	0.534
MOD_CK1_1	641	647	PF00069	0.780
MOD_CK1_1	679	685	PF00069	0.704
MOD_CK1_1	689	695	PF00069	0.568
MOD_CK1_1	717	723	PF00069	0.737
MOD_CK1_1	733	739	PF00069	0.748
MOD_CK2_1	294	300	PF00069	0.467
MOD_CK2_1	301	307	PF00069	0.498
MOD_CK2_1	363	369	PF00069	0.678
MOD_CK2_1	386	392	PF00069	0.590
MOD_CK2_1	538	544	PF00069	0.550
MOD_CK2_1	592	598	PF00069	0.487
MOD_CK2_1	728	734	PF00069	0.745
MOD_GlcNHglycan	18	21	PF01048	0.769
MOD_GlcNHglycan	205	208	PF01048	0.355
MOD_GlcNHglycan	365	368	PF01048	0.644
MOD_GlcNHglycan	447	451	PF01048	0.742
MOD_GlcNHglycan	470	473	PF01048	0.704
MOD_GlcNHglycan	476	479	PF01048	0.627
MOD_GlcNHglycan	569	572	PF01048	0.541
MOD_GlcNHglycan	622	625	PF01048	0.713
MOD_GlcNHglycan	626	629	PF01048	0.725
MOD_GlcNHglycan	635	638	PF01048	0.768
MOD_GlcNHglycan	640	643	PF01048	0.789
MOD_GlcNHglycan	681	684	PF01048	0.751
MOD_GlcNHglycan	714	717	PF01048	0.699
MOD_GlcNHglycan	719	722	PF01048	0.710
MOD_GlcNHglycan	73	76	PF01048	0.447
MOD_GlcNHglycan	730	733	PF01048	0.669
MOD_GlcNHglycan	749	752	PF01048	0.543
MOD_GlcNHglycan	755	758	PF01048	0.737
MOD_GSK3_1	189	196	PF00069	0.335
MOD_GSK3_1	252	259	PF00069	0.305
MOD_GSK3_1	294	301	PF00069	0.473
MOD_GSK3_1	309	316	PF00069	0.401
MOD_GSK3_1	363	370	PF00069	0.641
MOD_GSK3_1	459	466	PF00069	0.662
MOD_GSK3_1	470	477	PF00069	0.575
MOD_GSK3_1	494	501	PF00069	0.758
MOD_GSK3_1	508	515	PF00069	0.689
MOD_GSK3_1	592	599	PF00069	0.448
MOD_GSK3_1	6	13	PF00069	0.714
MOD_GSK3_1	606	613	PF00069	0.546
MOD_GSK3_1	620	627	PF00069	0.645
MOD_GSK3_1	686	693	PF00069	0.598
MOD_GSK3_1	728	735	PF00069	0.689
MOD_GSK3_1	743	750	PF00069	0.588
MOD_N-GLC_1	181	186	PF02516	0.480
MOD_N-GLC_1	522	527	PF02516	0.698
MOD_N-GLC_1	610	615	PF02516	0.665
MOD_N-GLC_2	95	97	PF02516	0.335
MOD_NEK2_1	166	171	PF00069	0.415
MOD_NEK2_1	178	183	PF00069	0.309
MOD_NEK2_1	189	194	PF00069	0.239
MOD_NEK2_1	241	246	PF00069	0.377
MOD_NEK2_1	294	299	PF00069	0.511
MOD_NEK2_1	32	37	PF00069	0.475
MOD_NEK2_1	397	402	PF00069	0.584
MOD_NEK2_1	463	468	PF00069	0.633
MOD_NEK2_1	474	479	PF00069	0.581
MOD_NEK2_1	508	513	PF00069	0.681
MOD_NEK2_1	592	597	PF00069	0.477
MOD_NEK2_1	688	693	PF00069	0.768
MOD_NEK2_2	298	303	PF00069	0.503
MOD_NEK2_2	459	464	PF00069	0.742
MOD_PIKK_1	397	403	PF00454	0.581
MOD_PIKK_1	515	521	PF00454	0.742
MOD_PIKK_1	653	659	PF00454	0.599
MOD_PIKK_1	690	696	PF00454	0.749
MOD_PK_1	309	315	PF00069	0.536
MOD_PK_1	529	535	PF00069	0.663
MOD_PKA_1	615	621	PF00069	0.664
MOD_PKA_2	131	137	PF00069	0.447
MOD_PKA_2	275	281	PF00069	0.335
MOD_PKA_2	294	300	PF00069	0.448
MOD_PKA_2	38	44	PF00069	0.447
MOD_PKA_2	463	469	PF00069	0.729
MOD_PKA_2	593	599	PF00069	0.484
MOD_PKA_2	615	621	PF00069	0.682
MOD_Plk_1	235	241	PF00069	0.355
MOD_Plk_1	441	447	PF00069	0.772
MOD_Plk_1	733	739	PF00069	0.682
MOD_Plk_4	298	304	PF00069	0.501
MOD_Plk_4	309	315	PF00069	0.406
MOD_Plk_4	32	38	PF00069	0.484
MOD_Plk_4	470	476	PF00069	0.738
MOD_Plk_4	769	775	PF00069	0.502
MOD_ProDKin_1	102	108	PF00069	0.355
MOD_ProDKin_1	193	199	PF00069	0.335
MOD_ProDKin_1	399	405	PF00069	0.578
MOD_ProDKin_1	584	590	PF00069	0.485
MOD_ProDKin_1	606	612	PF00069	0.559
MOD_ProDKin_1	760	766	PF00069	0.616
MOD_SUMO_for_1	160	163	PF00179	0.335
MOD_SUMO_rev_2	74	81	PF00179	0.385
MOD_SUMO_rev_2	783	793	PF00179	0.564
TRG_DiLeu_BaEn_2	542	548	PF01217	0.522
TRG_DiLeu_BaEn_3	543	549	PF01217	0.542
TRG_DiLeu_BaLyEn_6	237	242	PF01217	0.447
TRG_DiLeu_BaLyEn_6	587	592	PF01217	0.477
TRG_ENDOCYTIC_2	100	103	PF00928	0.373
TRG_ENDOCYTIC_2	110	113	PF00928	0.335
TRG_ENDOCYTIC_2	122	125	PF00928	0.335
TRG_ENDOCYTIC_2	225	228	PF00928	0.335
TRG_ENDOCYTIC_2	344	347	PF00928	0.355
TRG_ENDOCYTIC_2	573	576	PF00928	0.403
TRG_ENDOCYTIC_2	61	64	PF00928	0.447
TRG_ER_diArg_1	351	354	PF00400	0.533
TRG_ER_diArg_1	503	505	PF00400	0.752
TRG_ER_diArg_1	669	672	PF00400	0.762
TRG_NES_CRM1_1	355	369	PF08389	0.602
TRG_NLS_MonoExtN_4	245	251	PF00514	0.408
TRG_NLS_MonoExtN_4	49	55	PF00514	0.408
TRG_Pf-PMV_PEXEL_1	137	141	PF00026	0.363
TRG_Pf-PMV_PEXEL_1	158	163	PF00026	0.335
TRG_Pf-PMV_PEXEL_1	262	266	PF00026	0.335

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I379	Leptomonas seymouri	70%	99%
A0A1X0P022	Trypanosomatidae	61%	100%
A0A3R7MPF2	Trypanosoma rangeli	61%	100%
A0A3S7X2Q3	Leishmania donovani	95%	100%
A4HHK1	Leishmania braziliensis	86%	100%
A4I4Q9	Leishmania infantum	95%	100%
C9ZLF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E9AE64	Leishmania major	96%	100%
V5BL06	Trypanosoma cruzi	58%	100%