Polynucleotide adenylyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

poly(A) polymerase, putative

Species:

Leishmania mexicana

UniProt:

E9ALG4_LEIMU

TriTrypDb:

LmxM.08_29.2600

Length:

500

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	7
GO:0005737	cytoplasm	2	7
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7
GO:0110165	cellular anatomical entity	1	7
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

E9ALG4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ALG4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006396	RNA processing	6	7
GO:0006397	mRNA processing	7	7
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0008152	metabolic process	1	7
GO:0009987	cellular process	1	7
GO:0016070	RNA metabolic process	5	7
GO:0016071	mRNA metabolic process	6	7
GO:0031123	RNA 3'-end processing	7	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0043170	macromolecule metabolic process	3	7
GO:0043631	RNA polyadenylation	6	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0046483	heterocycle metabolic process	3	7
GO:0071076	RNA 3' uridylation	8	7
GO:0071704	organic substance metabolic process	2	7
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7
GO:0006378	mRNA polyadenylation	7	1
GO:0031124	mRNA 3'-end processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0003676	nucleic acid binding	3	7
GO:0003723	RNA binding	4	7
GO:0003824	catalytic activity	1	7
GO:0004652	obsolete polynucleotide adenylyltransferase activity	6	7
GO:0005488	binding	1	7
GO:0005524	ATP binding	5	7
GO:0016740	transferase activity	2	7
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	7
GO:0016779	nucleotidyltransferase activity	4	7
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0032553	ribonucleotide binding	3	7
GO:0032555	purine ribonucleotide binding	4	7
GO:0032559	adenyl ribonucleotide binding	5	7
GO:0035639	purine ribonucleoside triphosphate binding	4	7
GO:0036094	small molecule binding	2	7
GO:0043167	ion binding	2	7
GO:0043168	anion binding	3	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7
GO:0050265	RNA uridylyltransferase activity	4	7
GO:0070566	adenylyltransferase activity	5	7
GO:0070569	uridylyltransferase activity	5	7
GO:0097159	organic cyclic compound binding	2	7
GO:0097367	carbohydrate derivative binding	2	7
GO:0140098	catalytic activity, acting on RNA	3	7
GO:0140640	catalytic activity, acting on a nucleic acid	2	7
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	182	184	PF00675	0.352
CLV_NRD_NRD_1	204	206	PF00675	0.212
CLV_NRD_NRD_1	30	32	PF00675	0.582
CLV_NRD_NRD_1	409	411	PF00675	0.586
CLV_PCSK_KEX2_1	181	183	PF00082	0.352
CLV_PCSK_KEX2_1	204	206	PF00082	0.258
CLV_PCSK_KEX2_1	30	32	PF00082	0.582
CLV_PCSK_KEX2_1	409	411	PF00082	0.580
CLV_PCSK_PC7_1	405	411	PF00082	0.567
CLV_PCSK_SKI1_1	175	179	PF00082	0.404
CLV_PCSK_SKI1_1	196	200	PF00082	0.340
CLV_PCSK_SKI1_1	241	245	PF00082	0.323
CLV_PCSK_SKI1_1	30	34	PF00082	0.365
CLV_PCSK_SKI1_1	317	321	PF00082	0.422
CLV_PCSK_SKI1_1	405	409	PF00082	0.616
CLV_PCSK_SKI1_1	410	414	PF00082	0.517
CLV_PCSK_SKI1_1	419	423	PF00082	0.269
CLV_PCSK_SKI1_1	49	53	PF00082	0.142
CLV_Separin_Metazoa	153	157	PF03568	0.492
CLV_Separin_Metazoa	318	322	PF03568	0.404
CLV_Separin_Metazoa	402	406	PF03568	0.495
CLV_Separin_Metazoa	43	47	PF03568	0.375
DEG_APCC_DBOX_1	48	56	PF00400	0.379
DEG_Nend_UBRbox_2	1	3	PF02207	0.589
DOC_AGCK_PIF_2	441	446	PF00069	0.576
DOC_CYCLIN_RxL_1	179	189	PF00134	0.375
DOC_CYCLIN_RxL_1	314	323	PF00134	0.492
DOC_CYCLIN_RxL_1	46	53	PF00134	0.375
DOC_CYCLIN_yCln2_LP_2	184	190	PF00134	0.317
DOC_CYCLIN_yCln2_LP_2	336	342	PF00134	0.528
DOC_MAPK_DCC_7	253	262	PF00069	0.317
DOC_MAPK_gen_1	181	188	PF00069	0.404
DOC_MAPK_gen_1	253	262	PF00069	0.404
DOC_MAPK_MEF2A_6	181	188	PF00069	0.404
DOC_MAPK_MEF2A_6	309	316	PF00069	0.492
DOC_MAPK_MEF2A_6	347	354	PF00069	0.523
DOC_MAPK_NFAT4_5	181	189	PF00069	0.492
DOC_MAPK_NFAT4_5	309	317	PF00069	0.492
DOC_PP1_RVXF_1	467	473	PF00149	0.450
DOC_PP2B_LxvP_1	184	187	PF13499	0.321
DOC_PP4_FxxP_1	395	398	PF00568	0.567
DOC_PP4_MxPP_1	277	280	PF00568	0.492
DOC_SPAK_OSR1_1	347	351	PF12202	0.530
DOC_USP7_MATH_1	23	27	PF00917	0.457
DOC_USP7_MATH_1	234	238	PF00917	0.200
DOC_USP7_MATH_1	58	62	PF00917	0.527
DOC_USP7_MATH_1	90	94	PF00917	0.592
DOC_WW_Pin1_4	480	485	PF00397	0.549
LIG_14-3-3_CanoR_1	168	172	PF00244	0.404
LIG_14-3-3_CanoR_1	241	247	PF00244	0.418
LIG_14-3-3_CanoR_1	30	38	PF00244	0.547
LIG_14-3-3_CanoR_1	409	415	PF00244	0.546
LIG_14-3-3_CanoR_1	469	473	PF00244	0.487
LIG_BIR_III_2	12	16	PF00653	0.474
LIG_BRCT_BRCA1_1	417	421	PF00533	0.459
LIG_BRCT_BRCA1_1	98	102	PF00533	0.580
LIG_BRCT_BRCA1_2	98	104	PF00533	0.383
LIG_deltaCOP1_diTrp_1	282	291	PF00928	0.492
LIG_EH_1	455	459	PF12763	0.637
LIG_FHA_1	149	155	PF00498	0.492
LIG_FHA_1	39	45	PF00498	0.422
LIG_FHA_2	145	151	PF00498	0.455
LIG_FHA_2	174	180	PF00498	0.352
LIG_FHA_2	253	259	PF00498	0.411
LIG_FHA_2	80	86	PF00498	0.508
LIG_GBD_Chelix_1	47	55	PF00786	0.375
LIG_LIR_Gen_1	137	148	PF02991	0.520
LIG_LIR_Gen_1	157	166	PF02991	0.142
LIG_LIR_Gen_1	189	198	PF02991	0.319
LIG_LIR_Gen_1	338	348	PF02991	0.379
LIG_LIR_Gen_1	99	109	PF02991	0.522
LIG_LIR_Nem_3	12	17	PF02991	0.544
LIG_LIR_Nem_3	130	134	PF02991	0.520
LIG_LIR_Nem_3	137	143	PF02991	0.483
LIG_LIR_Nem_3	157	162	PF02991	0.142
LIG_LIR_Nem_3	189	194	PF02991	0.319
LIG_LIR_Nem_3	289	294	PF02991	0.354
LIG_LIR_Nem_3	334	340	PF02991	0.371
LIG_LIR_Nem_3	438	444	PF02991	0.563
LIG_LIR_Nem_3	471	476	PF02991	0.543
LIG_LIR_Nem_3	99	105	PF02991	0.525
LIG_LRP6_Inhibitor_1	169	175	PF00058	0.492
LIG_MLH1_MIPbox_1	98	102	PF16413	0.508
LIG_MYND_1	292	296	PF01753	0.404
LIG_NRBOX	315	321	PF00104	0.375
LIG_NRBOX	51	57	PF00104	0.425
LIG_PDZ_Class_1	495	500	PF00595	0.660
LIG_Pex14_1	66	70	PF04695	0.467
LIG_Pex14_2	101	105	PF04695	0.506
LIG_REV1ctd_RIR_1	420	430	PF16727	0.559
LIG_REV1ctd_RIR_1	99	105	PF16727	0.467
LIG_SH2_CRK	131	135	PF00017	0.592
LIG_SH2_CRK	191	195	PF00017	0.475
LIG_SH2_CRK	208	212	PF00017	0.211
LIG_SH2_CRK	476	480	PF00017	0.591
LIG_SH2_SRC	374	377	PF00017	0.536
LIG_SH2_STAP1	191	195	PF00017	0.404
LIG_SH2_STAP1	333	337	PF00017	0.536
LIG_SH2_STAP1	489	493	PF00017	0.549
LIG_SH2_STAT5	212	215	PF00017	0.352
LIG_SH2_STAT5	247	250	PF00017	0.404
LIG_SH2_STAT5	374	377	PF00017	0.405
LIG_SH2_STAT5	70	73	PF00017	0.508
LIG_SH3_3	160	166	PF00018	0.492
LIG_SH3_3	272	278	PF00018	0.363
LIG_SH3_3	290	296	PF00018	0.317
LIG_SH3_3	326	332	PF00018	0.200
LIG_SH3_3	397	403	PF00018	0.467
LIG_SUMO_SIM_anti_2	115	121	PF11976	0.508
LIG_SUMO_SIM_anti_2	157	164	PF11976	0.492
LIG_SUMO_SIM_anti_2	258	267	PF11976	0.317
LIG_SUMO_SIM_anti_2	84	90	PF11976	0.517
LIG_SUMO_SIM_par_1	115	121	PF11976	0.592
LIG_SUMO_SIM_par_1	87	93	PF11976	0.508
LIG_WW_3	402	406	PF00397	0.495
MOD_CK1_1	161	167	PF00069	0.363
MOD_CK1_1	471	477	PF00069	0.485
MOD_CK1_1	95	101	PF00069	0.532
MOD_CK2_1	173	179	PF00069	0.375
MOD_CK2_1	432	438	PF00069	0.492
MOD_GlcNHglycan	107	110	PF01048	0.362
MOD_GlcNHglycan	120	123	PF01048	0.332
MOD_GlcNHglycan	309	312	PF01048	0.404
MOD_GlcNHglycan	333	336	PF01048	0.430
MOD_GlcNHglycan	438	441	PF01048	0.387
MOD_GlcNHglycan	462	465	PF01048	0.532
MOD_GlcNHglycan	489	492	PF01048	0.550
MOD_GlcNHglycan	59	63	PF01048	0.374
MOD_GlcNHglycan	74	77	PF01048	0.246
MOD_GlcNHglycan	92	95	PF01048	0.392
MOD_GSK3_1	144	151	PF00069	0.470
MOD_GSK3_1	258	265	PF00069	0.331
MOD_GSK3_1	30	37	PF00069	0.439
MOD_GSK3_1	353	360	PF00069	0.445
MOD_GSK3_1	432	439	PF00069	0.375
MOD_GSK3_1	446	453	PF00069	0.506
MOD_GSK3_1	92	99	PF00069	0.505
MOD_N-GLC_1	460	465	PF02516	0.518
MOD_N-GLC_1	96	101	PF02516	0.339
MOD_NEK2_1	242	247	PF00069	0.414
MOD_NEK2_1	495	500	PF00069	0.543
MOD_NEK2_1	72	77	PF00069	0.508
MOD_NEK2_2	468	473	PF00069	0.537
MOD_PIKK_1	64	70	PF00454	0.534
MOD_PKA_1	30	36	PF00069	0.479
MOD_PKA_2	167	173	PF00069	0.358
MOD_PKA_2	252	258	PF00069	0.492
MOD_PKA_2	297	303	PF00069	0.446
MOD_PKA_2	30	36	PF00069	0.556
MOD_PKA_2	468	474	PF00069	0.535
MOD_Plk_1	234	240	PF00069	0.200
MOD_Plk_1	364	370	PF00069	0.449
MOD_Plk_1	460	466	PF00069	0.515
MOD_Plk_1	5	11	PF00069	0.501
MOD_Plk_1	96	102	PF00069	0.592
MOD_Plk_2-3	34	40	PF00069	0.498
MOD_Plk_4	158	164	PF00069	0.365
MOD_Plk_4	234	240	PF00069	0.255
MOD_Plk_4	258	264	PF00069	0.492
MOD_Plk_4	324	330	PF00069	0.200
MOD_Plk_4	468	474	PF00069	0.448
MOD_Plk_4	5	11	PF00069	0.548
MOD_Plk_4	96	102	PF00069	0.539
MOD_ProDKin_1	480	486	PF00069	0.546
TRG_DiLeu_BaEn_1	43	48	PF01217	0.375
TRG_DiLeu_BaEn_2	344	350	PF01217	0.458
TRG_DiLeu_BaLyEn_6	122	127	PF01217	0.592
TRG_ENDOCYTIC_2	131	134	PF00928	0.592
TRG_ENDOCYTIC_2	191	194	PF00928	0.414
TRG_ENDOCYTIC_2	208	211	PF00928	0.194
TRG_ENDOCYTIC_2	333	336	PF00928	0.453
TRG_ENDOCYTIC_2	340	343	PF00928	0.370
TRG_ENDOCYTIC_2	476	479	PF00928	0.591
TRG_ER_diArg_1	180	183	PF00400	0.356
TRG_ER_diArg_1	29	31	PF00400	0.557
TRG_Pf-PMV_PEXEL_1	175	179	PF00026	0.492
TRG_Pf-PMV_PEXEL_1	49	53	PF00026	0.308

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2K1	Leptomonas seymouri	26%	68%
A0A0S4IKH6	Bodo saltans	35%	91%
A0A1X0NP17	Trypanosomatidae	28%	72%
A0A3R7KE83	Trypanosoma rangeli	29%	78%
A4H7T8	Leishmania braziliensis	26%	71%
A4HHQ8	Leishmania braziliensis	88%	100%
A4I4T6	Leishmania infantum	95%	100%
C9ZKY5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
C9ZSW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	73%
E9AEC2	Leishmania major	94%	100%
E9APW5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	71%
O42617	Candida albicans (strain SC5314 / ATCC MYA-2876)	30%	90%
P25500	Bos taurus	30%	68%
P29468	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	88%
P51003	Homo sapiens	30%	67%
P51004	Xenopus laevis	29%	70%
Q10295	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	88%
Q51D88	Entamoeba histolytica	24%	96%
Q56XM9	Arabidopsis thaliana	27%	99%
Q61183	Mus musculus	30%	68%
Q6PCL9	Mus musculus	30%	68%
Q8VYW1	Arabidopsis thaliana	28%	67%
Q9BWT3	Homo sapiens	31%	68%
Q9LMT2	Arabidopsis thaliana	25%	70%
Q9NRJ5	Homo sapiens	29%	78%
Q9UW26	Candida albicans (strain SC5314 / ATCC MYA-2876)	29%	90%
Q9WVP6	Mus musculus	30%	78%