LeishMANIAdb
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Putative serine/threonine-protein kinase Nek3

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Putative serine/threonine-protein kinase Nek3
Gene product:
serine/threonine-protein kinase Nek, putative
Species:
Leishmania mexicana
UniProt:
E9ALF7_LEIMU
TriTrypDb:
LmxM.08_29.2670
Length:
522

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. None of them appear to be TM.

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 6
Forrest at al. (procyclic) no yes: 6
Silverman et al. no yes: 0
Pissara et al. no yes: 15
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 5
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 20
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 1, no: 67
NetGPI no yes: 0, no: 68
Cellular components
Term Name Level Count
GO:0016020 membrane 2 1
GO:0110165 cellular anatomical entity 1 7
GO:0005815 microtubule organizing center 2 1
GO:0005929 cilium 4 1
GO:0036064 ciliary basal body 3 1
GO:0042995 cell projection 2 1
GO:0043226 organelle 2 2
GO:0043227 membrane-bounded organelle 3 2
GO:0120025 plasma membrane bounded cell projection 3 1
GO:0005737 cytoplasm 2 5
GO:0005930 axoneme 2 1
GO:0005952 cAMP-dependent protein kinase complex 3 1
GO:0031974 membrane-enclosed lumen 2 2
GO:0031981 nuclear lumen 5 2
GO:0032838 plasma membrane bounded cell projection cytoplasm 4 2
GO:0032991 protein-containing complex 1 1
GO:0043233 organelle lumen 3 2
GO:0061695 transferase complex, transferring phosphorus-containing groups 4 1
GO:0070013 intracellular organelle lumen 4 2
GO:0097014 ciliary plasm 5 2
GO:0099568 cytoplasmic region 3 2
GO:0140535 intracellular protein-containing complex 2 1
GO:1902494 catalytic complex 2 1
GO:1902554 serine/threonine protein kinase complex 6 1
GO:1902911 protein kinase complex 5 1
GO:1990234 transferase complex 3 1
GO:0005634 nucleus 5 1
GO:0043229 intracellular organelle 3 1
GO:0043231 intracellular membrane-bounded organelle 4 1

Expansion

Sequence features

E9ALF7
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ALF7

Function

Biological processes
Term Name Level Count
GO:0006468 protein phosphorylation 5 69
GO:0006793 phosphorus metabolic process 3 69
GO:0006796 phosphate-containing compound metabolic process 4 69
GO:0006807 nitrogen compound metabolic process 2 69
GO:0008152 metabolic process 1 69
GO:0009987 cellular process 1 69
GO:0016310 phosphorylation 5 69
GO:0019538 protein metabolic process 3 69
GO:0036211 protein modification process 4 69
GO:0043170 macromolecule metabolic process 3 69
GO:0043412 macromolecule modification 4 69
GO:0044237 cellular metabolic process 2 69
GO:0044238 primary metabolic process 2 69
GO:0071704 organic substance metabolic process 2 69
GO:1901564 organonitrogen compound metabolic process 3 69
GO:0007165 signal transduction 2 5
GO:0035556 intracellular signal transduction 3 4
GO:0050789 regulation of biological process 2 5
GO:0050794 regulation of cellular process 3 5
GO:0065007 biological regulation 1 5
GO:0018105 peptidyl-serine phosphorylation 6 3
GO:0018193 peptidyl-amino acid modification 5 3
GO:0018209 peptidyl-serine modification 6 3
GO:0000281 mitotic cytokinesis 4 1
GO:0000910 cytokinesis 3 1
GO:0022402 cell cycle process 2 1
GO:0061640 cytoskeleton-dependent cytokinesis 4 1
GO:1903047 mitotic cell cycle process 3 1
GO:0009266 response to temperature stimulus 3 1
GO:0009628 response to abiotic stimulus 2 1
GO:0050896 response to stimulus 1 1
Molecular functions
Term Name Level Count
GO:0000166 nucleotide binding 3 69
GO:0003824 catalytic activity 1 69
GO:0004672 protein kinase activity 3 69
GO:0004674 protein serine/threonine kinase activity 4 54
GO:0005488 binding 1 69
GO:0005524 ATP binding 5 69
GO:0016301 kinase activity 4 69
GO:0016740 transferase activity 2 69
GO:0016772 transferase activity, transferring phosphorus-containing groups 3 69
GO:0016773 phosphotransferase activity, alcohol group as acceptor 4 69
GO:0017076 purine nucleotide binding 4 69
GO:0030554 adenyl nucleotide binding 5 69
GO:0032553 ribonucleotide binding 3 69
GO:0032555 purine ribonucleotide binding 4 69
GO:0032559 adenyl ribonucleotide binding 5 69
GO:0035639 purine ribonucleoside triphosphate binding 4 69
GO:0036094 small molecule binding 2 69
GO:0043167 ion binding 2 69
GO:0043168 anion binding 3 69
GO:0097159 organic cyclic compound binding 2 69
GO:0097367 carbohydrate derivative binding 2 69
GO:0140096 catalytic activity, acting on a protein 2 69
GO:1901265 nucleoside phosphate binding 3 69
GO:1901363 heterocyclic compound binding 2 69
GO:0004707 MAP kinase activity 5 8
GO:0043169 cation binding 3 5
GO:0046872 metal ion binding 4 5
GO:0004690 cyclic nucleotide-dependent protein kinase activity 5 1
GO:0004691 cAMP-dependent protein kinase activity 6 1
GO:0004140 dephospho-CoA kinase activity 5 1

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 503 507 PF00656 0.390
CLV_NRD_NRD_1 215 217 PF00675 0.250
CLV_NRD_NRD_1 260 262 PF00675 0.315
CLV_NRD_NRD_1 443 445 PF00675 0.507
CLV_PCSK_KEX2_1 215 217 PF00082 0.261
CLV_PCSK_KEX2_1 260 262 PF00082 0.384
CLV_PCSK_KEX2_1 442 444 PF00082 0.482
CLV_PCSK_PC7_1 256 262 PF00082 0.346
CLV_PCSK_SKI1_1 142 146 PF00082 0.386
CLV_PCSK_SKI1_1 251 255 PF00082 0.366
CLV_PCSK_SKI1_1 385 389 PF00082 0.557
CLV_PCSK_SKI1_1 516 520 PF00082 0.628
DEG_SPOP_SBC_1 359 363 PF00917 0.606
DOC_CKS1_1 179 184 PF01111 0.284
DOC_CYCLIN_RxL_1 431 439 PF00134 0.461
DOC_CYCLIN_yCln2_LP_2 214 220 PF00134 0.284
DOC_CYCLIN_yCln2_LP_2 272 278 PF00134 0.476
DOC_MAPK_gen_1 142 151 PF00069 0.378
DOC_MAPK_gen_1 58 65 PF00069 0.288
DOC_PP2B_LxvP_1 272 275 PF13499 0.403
DOC_PP4_FxxP_1 295 298 PF00568 0.445
DOC_PP4_FxxP_1 470 473 PF00568 0.546
DOC_PP4_FxxP_1 81 84 PF00568 0.332
DOC_USP7_MATH_1 359 363 PF00917 0.647
DOC_USP7_MATH_1 383 387 PF00917 0.719
DOC_USP7_MATH_1 49 53 PF00917 0.367
DOC_USP7_MATH_1 85 89 PF00917 0.350
DOC_USP7_UBL2_3 154 158 PF12436 0.278
DOC_USP7_UBL2_3 385 389 PF12436 0.566
DOC_WW_Pin1_4 110 115 PF00397 0.221
DOC_WW_Pin1_4 178 183 PF00397 0.332
DOC_WW_Pin1_4 361 366 PF00397 0.602
DOC_WW_Pin1_4 401 406 PF00397 0.563
LIG_14-3-3_CanoR_1 442 448 PF00244 0.758
LIG_14-3-3_CanoR_1 492 500 PF00244 0.507
LIG_APCC_ABBA_1 268 273 PF00400 0.453
LIG_APCC_ABBA_1 335 340 PF00400 0.538
LIG_APCC_ABBAyCdc20_2 444 450 PF00400 0.504
LIG_BIR_II_1 1 5 PF00653 0.441
LIG_BRCT_BRCA1_1 146 150 PF00533 0.223
LIG_deltaCOP1_diTrp_1 200 208 PF00928 0.301
LIG_EH1_1 161 169 PF00400 0.346
LIG_FHA_1 115 121 PF00498 0.381
LIG_FHA_1 134 140 PF00498 0.473
LIG_FHA_1 144 150 PF00498 0.283
LIG_FHA_1 24 30 PF00498 0.310
LIG_FHA_1 38 44 PF00498 0.357
LIG_FHA_1 407 413 PF00498 0.569
LIG_FHA_1 424 430 PF00498 0.535
LIG_FHA_2 261 267 PF00498 0.289
LIG_FHA_2 430 436 PF00498 0.484
LIG_FHA_2 98 104 PF00498 0.275
LIG_LIR_Apic_2 181 187 PF02991 0.377
LIG_LIR_Apic_2 303 309 PF02991 0.500
LIG_LIR_Apic_2 468 473 PF02991 0.536
LIG_LIR_Apic_2 79 84 PF02991 0.332
LIG_LIR_Gen_1 14 21 PF02991 0.421
LIG_LIR_Gen_1 221 231 PF02991 0.378
LIG_LIR_Gen_1 336 345 PF02991 0.584
LIG_LIR_Gen_1 94 101 PF02991 0.399
LIG_LIR_Nem_3 14 18 PF02991 0.433
LIG_LIR_Nem_3 221 227 PF02991 0.401
LIG_LIR_Nem_3 27 33 PF02991 0.521
LIG_LIR_Nem_3 336 341 PF02991 0.473
LIG_LIR_Nem_3 513 518 PF02991 0.466
LIG_LIR_Nem_3 94 98 PF02991 0.422
LIG_NRP_CendR_1 519 522 PF00754 0.421
LIG_Pex14_2 77 81 PF04695 0.223
LIG_PTAP_UEV_1 364 369 PF05743 0.496
LIG_SH2_CRK 30 34 PF00017 0.329
LIG_SH2_STAP1 302 306 PF00017 0.478
LIG_SH2_STAT5 184 187 PF00017 0.321
LIG_SH2_STAT5 302 305 PF00017 0.479
LIG_SH2_STAT5 41 44 PF00017 0.265
LIG_SH2_STAT5 95 98 PF00017 0.469
LIG_SH3_3 273 279 PF00018 0.433
LIG_SH3_3 310 316 PF00018 0.670
LIG_SH3_3 362 368 PF00018 0.552
LIG_SH3_3 66 72 PF00018 0.284
LIG_SUMO_SIM_anti_2 62 67 PF11976 0.287
LIG_SUMO_SIM_anti_2 88 94 PF11976 0.291
LIG_SUMO_SIM_par_1 456 464 PF11976 0.509
LIG_TRAF2_1 263 266 PF00917 0.260
LIG_TRAF2_1 52 55 PF00917 0.330
LIG_TRAF2_1 59 62 PF00917 0.330
LIG_WRC_WIRS_1 428 433 PF05994 0.420
MOD_CDK_SPxxK_3 401 408 PF00069 0.512
MOD_CK1_1 23 29 PF00069 0.405
MOD_CK1_1 277 283 PF00069 0.401
MOD_CK1_1 427 433 PF00069 0.468
MOD_CK1_1 453 459 PF00069 0.795
MOD_CK1_1 471 477 PF00069 0.534
MOD_CK2_1 230 236 PF00069 0.246
MOD_CK2_1 241 247 PF00069 0.229
MOD_CK2_1 260 266 PF00069 0.365
MOD_CK2_1 293 299 PF00069 0.530
MOD_CK2_1 378 384 PF00069 0.504
MOD_CK2_1 429 435 PF00069 0.647
MOD_CK2_1 466 472 PF00069 0.639
MOD_CK2_1 49 55 PF00069 0.351
MOD_GlcNHglycan 1 4 PF01048 0.494
MOD_GlcNHglycan 22 25 PF01048 0.370
MOD_GlcNHglycan 295 298 PF01048 0.452
MOD_GlcNHglycan 313 316 PF01048 0.638
MOD_GlcNHglycan 332 335 PF01048 0.596
MOD_GlcNHglycan 365 368 PF01048 0.610
MOD_GlcNHglycan 375 378 PF01048 0.669
MOD_GlcNHglycan 384 388 PF01048 0.689
MOD_GlcNHglycan 420 424 PF01048 0.498
MOD_GSK3_1 110 117 PF00069 0.505
MOD_GSK3_1 129 136 PF00069 0.308
MOD_GSK3_1 174 181 PF00069 0.336
MOD_GSK3_1 196 203 PF00069 0.289
MOD_GSK3_1 20 27 PF00069 0.342
MOD_GSK3_1 256 263 PF00069 0.312
MOD_GSK3_1 280 287 PF00069 0.454
MOD_GSK3_1 33 40 PF00069 0.262
MOD_GSK3_1 359 366 PF00069 0.700
MOD_GSK3_1 383 390 PF00069 0.596
MOD_GSK3_1 393 400 PF00069 0.651
MOD_GSK3_1 419 426 PF00069 0.484
MOD_N-GLC_1 33 38 PF02516 0.438
MOD_N-GLC_1 49 54 PF02516 0.298
MOD_NEK2_1 144 149 PF00069 0.350
MOD_NEK2_1 150 155 PF00069 0.354
MOD_NEK2_1 300 305 PF00069 0.498
MOD_NEK2_1 341 346 PF00069 0.595
MOD_NEK2_1 360 365 PF00069 0.542
MOD_NEK2_1 372 377 PF00069 0.542
MOD_NEK2_1 424 429 PF00069 0.516
MOD_NEK2_1 77 82 PF00069 0.304
MOD_PIKK_1 280 286 PF00454 0.420
MOD_PKA_1 260 266 PF00069 0.310
MOD_PKA_1 388 394 PF00069 0.619
MOD_PKA_1 44 50 PF00069 0.365
MOD_PKA_1 442 448 PF00069 0.552
MOD_PKA_2 260 266 PF00069 0.327
MOD_PKA_2 322 328 PF00069 0.833
MOD_PKA_2 442 448 PF00069 0.578
MOD_PKA_2 491 497 PF00069 0.472
MOD_PKA_2 77 83 PF00069 0.242
MOD_Plk_1 242 248 PF00069 0.208
MOD_Plk_1 37 43 PF00069 0.233
MOD_Plk_1 419 425 PF00069 0.501
MOD_Plk_1 450 456 PF00069 0.565
MOD_Plk_1 85 91 PF00069 0.265
MOD_Plk_2-3 97 103 PF00069 0.291
MOD_Plk_4 163 169 PF00069 0.242
MOD_Plk_4 174 180 PF00069 0.283
MOD_Plk_4 424 430 PF00069 0.472
MOD_Plk_4 443 449 PF00069 0.461
MOD_Plk_4 77 83 PF00069 0.233
MOD_ProDKin_1 110 116 PF00069 0.221
MOD_ProDKin_1 178 184 PF00069 0.332
MOD_ProDKin_1 361 367 PF00069 0.600
MOD_ProDKin_1 401 407 PF00069 0.556
MOD_SUMO_for_1 173 176 PF00179 0.291
MOD_SUMO_for_1 59 62 PF00179 0.233
MOD_SUMO_rev_2 446 454 PF00179 0.492
MOD_SUMO_rev_2 513 518 PF00179 0.466
TRG_DiLeu_BaLyEn_6 287 292 PF01217 0.431
TRG_ENDOCYTIC_2 15 18 PF00928 0.423
TRG_ENDOCYTIC_2 30 33 PF00928 0.478
TRG_ENDOCYTIC_2 515 518 PF00928 0.470
TRG_ENDOCYTIC_2 95 98 PF00928 0.426
TRG_ER_diArg_1 107 110 PF00400 0.268
TRG_ER_diArg_1 214 216 PF00400 0.247
TRG_ER_diArg_1 260 262 PF00400 0.338
TRG_ER_diArg_1 288 291 PF00400 0.451
TRG_ER_diArg_1 442 444 PF00400 0.499
TRG_NES_CRM1_1 221 234 PF08389 0.218
TRG_Pf-PMV_PEXEL_1 138 143 PF00026 0.417
TRG_Pf-PMV_PEXEL_1 290 294 PF00026 0.425
TRG_Pf-PMV_PEXEL_1 434 439 PF00026 0.493
TRG_PTS2 1 21 PF00400 0.375

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1PEV2 Leptomonas seymouri 65% 96%
A0A0S4JIJ6 Bodo saltans 26% 92%
A0A1X0P963 Trypanosomatidae 47% 100%
A0A3Q8IAQ1 Leishmania donovani 33% 100%
A0A3Q8IC87 Leishmania donovani 29% 100%
A0A3Q8IIG1 Leishmania donovani 33% 100%
A0A3Q8INQ4 Leishmania donovani 26% 100%
A0A3S5H5G0 Leishmania donovani 26% 100%
A0A3S5H789 Leishmania donovani 25% 100%
A0A3S5H7M0 Leishmania donovani 95% 100%
A0A3S7WTN9 Leishmania donovani 30% 100%
A0A3S7WWE7 Leishmania donovani 24% 100%
A0A3S7WYP4 Leishmania donovani 27% 100%
A0A3S7X2W3 Leishmania donovani 28% 100%
A0A3S7X2Z6 Leishmania donovani 26% 100%
A0A3S7X9R4 Leishmania donovani 29% 100%
A0A3S7XAL3 Leishmania donovani 25% 100%
A4H459 Leishmania braziliensis 26% 100%
A4H8C4 Leishmania braziliensis 31% 100%
A4HBL4 Leishmania braziliensis 24% 100%
A4HCE6 Leishmania braziliensis 34% 100%
A4HED7 Leishmania braziliensis 26% 100%
A4HH03 Leishmania braziliensis 32% 100%
A4HHN1 Leishmania braziliensis 28% 100%
A4HHR5 Leishmania braziliensis 76% 100%
A4HN71 Leishmania braziliensis 31% 100%
A4HSE2 Leishmania infantum 26% 100%
A4HWP5 Leishmania infantum 30% 100%
A4HYX6 Leishmania infantum 25% 100%
A4HZA2 Leishmania infantum 24% 100%
A4HZV1 Leishmania infantum 29% 100%
A4HZW8 Leishmania infantum 33% 100%
A4I140 Leishmania infantum 27% 100%
A4I1T4 Leishmania infantum 26% 100%
A4I435 Leishmania infantum 33% 100%
A4I4U6 Leishmania infantum 28% 100%
A4I4X5 Leishmania infantum 94% 100%
A4I564 Leishmania infantum 26% 100%
A4IBT4 Leishmania infantum 29% 100%
A4IBT9 Leishmania infantum 29% 100%
C9ZKX6 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 45% 100%
E9AEC9 Leishmania major 94% 100%
E9AKB7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 26% 100%
E9ALJ2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 28% 100%
E9AQF3 Leishmania mexicana (strain MHOM/GT/2001/U1103) 30% 100%
E9ARW9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 27% 100%
E9AUY2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 23% 100%
E9AVR5 Leishmania mexicana (strain MHOM/GT/2001/U1103) 28% 100%
E9AVS7 Leishmania mexicana (strain MHOM/GT/2001/U1103) 33% 100%
E9AX78 Leishmania mexicana (strain MHOM/GT/2001/U1103) 28% 100%
E9AXW8 Leishmania mexicana (strain MHOM/GT/2001/U1103) 26% 100%
E9B0C2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 34% 100%
E9B0G1 Leishmania mexicana (strain MHOM/GT/2001/U1103) 27% 100%
E9B6S4 Leishmania mexicana (strain MHOM/GT/2001/U1103) 30% 100%
E9B6S9 Leishmania mexicana (strain MHOM/GT/2001/U1103) 30% 100%
Q4Q7S2 Leishmania major 26% 100%
Q4Q7W2 Leishmania major 33% 100%
Q4Q9K2 Leishmania major 26% 100%
Q4QA98 Leishmania major 28% 100%
Q4QBQ2 Leishmania major 32% 100%
Q4QBR6 Leishmania major 29% 100%
Q4QCK0 Leishmania major 25% 100%
Q4QF23 Leishmania major 30% 100%
Q4QJJ0 Leishmania major 26% 100%
Q9GNR4 Leishmania major 29% 100%
Q9Y077 Leishmania major 28% 100%
V5C234 Trypanosoma cruzi 46% 96%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2025 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS