Putative tRNA-methyl transferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA-methyl transferase, putative

Species:

Leishmania mexicana

UniProt:

E9AL23_LEIMU

TriTrypDb:

LmxM.07.0130

Length:

603

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AL23

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AL23

Function

Biological processes
Term	Name	Level	Count
GO:0002097	tRNA wobble base modification	7	1
GO:0002098	tRNA wobble uridine modification	8	1
GO:0002143	tRNA wobble position uridine thiolation	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0034227	tRNA thio-modification	7	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004808	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity	6	8
GO:0008168	methyltransferase activity	4	8
GO:0008173	RNA methyltransferase activity	4	8
GO:0008175	tRNA methyltransferase activity	5	8
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	8
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	8
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140101	catalytic activity, acting on a tRNA	4	8
GO:0140640	catalytic activity, acting on a nucleic acid	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	575	579	PF00656	0.769
CLV_NRD_NRD_1	187	189	PF00675	0.360
CLV_NRD_NRD_1	221	223	PF00675	0.331
CLV_NRD_NRD_1	385	387	PF00675	0.632
CLV_NRD_NRD_1	415	417	PF00675	0.581
CLV_NRD_NRD_1	536	538	PF00675	0.351
CLV_PCSK_FUR_1	534	538	PF00082	0.305
CLV_PCSK_KEX2_1	187	189	PF00082	0.360
CLV_PCSK_KEX2_1	220	222	PF00082	0.329
CLV_PCSK_KEX2_1	384	386	PF00082	0.653
CLV_PCSK_KEX2_1	415	417	PF00082	0.581
CLV_PCSK_KEX2_1	536	538	PF00082	0.351
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.305
CLV_PCSK_SKI1_1	190	194	PF00082	0.221
CLV_PCSK_SKI1_1	222	226	PF00082	0.307
CLV_PCSK_SKI1_1	253	257	PF00082	0.170
CLV_PCSK_SKI1_1	349	353	PF00082	0.474
CLV_PCSK_SKI1_1	547	551	PF00082	0.281
CLV_PCSK_SKI1_1	74	78	PF00082	0.631
DEG_APCC_DBOX_1	385	393	PF00400	0.405
DEG_SPOP_SBC_1	344	348	PF00917	0.392
DOC_CKS1_1	94	99	PF01111	0.455
DOC_CYCLIN_RxL_1	125	136	PF00134	0.509
DOC_CYCLIN_yCln2_LP_2	399	405	PF00134	0.416
DOC_CYCLIN_yCln2_LP_2	481	487	PF00134	0.535
DOC_MAPK_gen_1	220	227	PF00069	0.505
DOC_MAPK_gen_1	302	310	PF00069	0.468
DOC_MAPK_gen_1	534	542	PF00069	0.560
DOC_MAPK_MEF2A_6	220	227	PF00069	0.505
DOC_MAPK_MEF2A_6	7	16	PF00069	0.541
DOC_PP1_RVXF_1	174	180	PF00149	0.537
DOC_PP1_RVXF_1	537	543	PF00149	0.552
DOC_PP2B_LxvP_1	337	340	PF13499	0.596
DOC_PP2B_LxvP_1	361	364	PF13499	0.576
DOC_PP2B_LxvP_1	481	484	PF13499	0.546
DOC_PP4_FxxP_1	310	313	PF00568	0.617
DOC_PP4_FxxP_1	45	48	PF00568	0.592
DOC_PP4_FxxP_1	542	545	PF00568	0.457
DOC_USP7_MATH_1	272	276	PF00917	0.537
DOC_USP7_MATH_1	284	288	PF00917	0.691
DOC_USP7_MATH_1	292	296	PF00917	0.341
DOC_USP7_MATH_1	344	348	PF00917	0.619
DOC_USP7_MATH_1	375	379	PF00917	0.523
DOC_USP7_MATH_1	436	440	PF00917	0.512
DOC_USP7_UBL2_3	70	74	PF12436	0.288
DOC_WW_Pin1_4	135	140	PF00397	0.550
DOC_WW_Pin1_4	547	552	PF00397	0.494
DOC_WW_Pin1_4	579	584	PF00397	0.707
DOC_WW_Pin1_4	594	599	PF00397	0.595
DOC_WW_Pin1_4	93	98	PF00397	0.450
LIG_14-3-3_CanoR_1	187	193	PF00244	0.542
LIG_14-3-3_CanoR_1	228	236	PF00244	0.482
LIG_14-3-3_CanoR_1	273	277	PF00244	0.547
LIG_14-3-3_CanoR_1	31	39	PF00244	0.429
LIG_14-3-3_CanoR_1	415	419	PF00244	0.568
LIG_BIR_II_1	1	5	PF00653	0.631
LIG_BIR_III_2	578	582	PF00653	0.770
LIG_BRCT_BRCA1_1	325	329	PF00533	0.644
LIG_Clathr_ClatBox_1	561	565	PF01394	0.375
LIG_CSL_BTD_1	479	482	PF09270	0.485
LIG_EVH1_1	337	341	PF00568	0.563
LIG_FHA_1	164	170	PF00498	0.470
LIG_FHA_1	181	187	PF00498	0.457
LIG_FHA_1	204	210	PF00498	0.488
LIG_FHA_1	23	29	PF00498	0.398
LIG_FHA_1	238	244	PF00498	0.452
LIG_FHA_1	280	286	PF00498	0.688
LIG_FHA_1	34	40	PF00498	0.296
LIG_FHA_1	346	352	PF00498	0.454
LIG_FHA_1	445	451	PF00498	0.359
LIG_FHA_2	33	39	PF00498	0.419
LIG_FHA_2	355	361	PF00498	0.484
LIG_Integrin_RGD_1	576	578	PF01839	0.616
LIG_LIR_Apic_2	206	210	PF02991	0.505
LIG_LIR_Apic_2	335	339	PF02991	0.526
LIG_LIR_Apic_2	476	482	PF02991	0.492
LIG_LIR_Gen_1	151	162	PF02991	0.486
LIG_LIR_Gen_1	85	95	PF02991	0.301
LIG_LIR_Nem_3	326	332	PF02991	0.605
LIG_LIR_Nem_3	395	399	PF02991	0.562
LIG_LIR_Nem_3	85	91	PF02991	0.534
LIG_LYPXL_SIV_4	426	434	PF13949	0.441
LIG_LYPXL_yS_3	396	399	PF13949	0.525
LIG_MYND_1	397	401	PF01753	0.481
LIG_MYND_3	559	563	PF01753	0.461
LIG_Pex14_2	99	103	PF04695	0.457
LIG_PROFILIN_1	338	344	PF00235	0.493
LIG_REV1ctd_RIR_1	96	105	PF16727	0.505
LIG_SH2_CRK	558	562	PF00017	0.450
LIG_SH2_CRK	88	92	PF00017	0.298
LIG_SH2_NCK_1	120	124	PF00017	0.488
LIG_SH2_NCK_1	88	92	PF00017	0.512
LIG_SH2_SRC	120	123	PF00017	0.537
LIG_SH2_SRC	336	339	PF00017	0.590
LIG_SH2_STAP1	203	207	PF00017	0.457
LIG_SH2_STAP1	403	407	PF00017	0.391
LIG_SH2_STAP1	468	472	PF00017	0.503
LIG_SH2_STAT3	149	152	PF00017	0.537
LIG_SH2_STAT3	468	471	PF00017	0.532
LIG_SH2_STAT5	149	152	PF00017	0.440
LIG_SH2_STAT5	336	339	PF00017	0.551
LIG_SH2_STAT5	428	431	PF00017	0.369
LIG_SH2_STAT5	468	471	PF00017	0.513
LIG_SH2_STAT5	95	98	PF00017	0.449
LIG_SH3_1	336	342	PF00018	0.645
LIG_SH3_3	308	314	PF00018	0.576
LIG_SH3_3	335	341	PF00018	0.607
LIG_SH3_3	430	436	PF00018	0.540
LIG_SH3_3	45	51	PF00018	0.606
LIG_SH3_3	578	584	PF00018	0.750
LIG_SH3_3	595	601	PF00018	0.348
LIG_SUMO_SIM_par_1	560	566	PF11976	0.525
LIG_TRAF2_1	357	360	PF00917	0.555
LIG_TRAF2_1	523	526	PF00917	0.560
LIG_TYR_ITIM	556	561	PF00017	0.360
LIG_UBA3_1	561	569	PF00899	0.508
LIG_WW_2	53	56	PF00397	0.357
MOD_CK1_1	191	197	PF00069	0.455
MOD_CK1_1	2	8	PF00069	0.677
MOD_CK1_1	22	28	PF00069	0.145
MOD_CK1_1	324	330	PF00069	0.660
MOD_CK1_1	347	353	PF00069	0.549
MOD_CK1_1	373	379	PF00069	0.625
MOD_CK1_1	440	446	PF00069	0.660
MOD_CK1_1	470	476	PF00069	0.507
MOD_CK1_1	597	603	PF00069	0.641
MOD_CK2_1	354	360	PF00069	0.465
MOD_CK2_1	494	500	PF00069	0.282
MOD_CK2_1	579	585	PF00069	0.714
MOD_GlcNHglycan	109	112	PF01048	0.424
MOD_GlcNHglycan	16	19	PF01048	0.365
MOD_GlcNHglycan	21	24	PF01048	0.332
MOD_GlcNHglycan	214	217	PF01048	0.366
MOD_GlcNHglycan	230	233	PF01048	0.318
MOD_GlcNHglycan	248	251	PF01048	0.287
MOD_GlcNHglycan	279	282	PF01048	0.695
MOD_GlcNHglycan	314	317	PF01048	0.622
MOD_GlcNHglycan	372	375	PF01048	0.632
MOD_GlcNHglycan	439	442	PF01048	0.542
MOD_GlcNHglycan	524	530	PF01048	0.431
MOD_GlcNHglycan	591	594	PF01048	0.410
MOD_GSK3_1	193	200	PF00069	0.392
MOD_GSK3_1	237	244	PF00069	0.330
MOD_GSK3_1	297	304	PF00069	0.450
MOD_GSK3_1	320	327	PF00069	0.680
MOD_GSK3_1	343	350	PF00069	0.584
MOD_GSK3_1	369	376	PF00069	0.667
MOD_GSK3_1	436	443	PF00069	0.571
MOD_GSK3_1	466	473	PF00069	0.477
MOD_N-GLC_1	444	449	PF02516	0.367
MOD_NEK2_1	172	177	PF00069	0.383
MOD_NEK2_1	186	191	PF00069	0.227
MOD_NEK2_1	193	198	PF00069	0.306
MOD_NEK2_1	345	350	PF00069	0.574
MOD_NEK2_1	466	471	PF00069	0.407
MOD_PIKK_1	297	303	PF00454	0.290
MOD_PIKK_1	373	379	PF00454	0.688
MOD_PIKK_1	467	473	PF00454	0.513
MOD_PIKK_1	5	11	PF00454	0.535
MOD_PK_1	188	194	PF00069	0.453
MOD_PKA_1	302	308	PF00069	0.477
MOD_PKA_1	385	391	PF00069	0.417
MOD_PKA_2	186	192	PF00069	0.455
MOD_PKA_2	272	278	PF00069	0.452
MOD_PKA_2	385	391	PF00069	0.649
MOD_PKA_2	414	420	PF00069	0.570
MOD_Plk_1	237	243	PF00069	0.310
MOD_Plk_1	444	450	PF00069	0.350
MOD_Plk_1	494	500	PF00069	0.498
MOD_Plk_4	193	199	PF00069	0.395
MOD_Plk_4	237	243	PF00069	0.310
MOD_ProDKin_1	135	141	PF00069	0.441
MOD_ProDKin_1	547	553	PF00069	0.361
MOD_ProDKin_1	579	585	PF00069	0.698
MOD_ProDKin_1	594	600	PF00069	0.605
MOD_ProDKin_1	93	99	PF00069	0.450
MOD_SUMO_rev_2	439	448	PF00179	0.658
TRG_DiLeu_BaLyEn_6	448	453	PF01217	0.458
TRG_DiLeu_BaLyEn_6	557	562	PF01217	0.440
TRG_ENDOCYTIC_2	396	399	PF00928	0.583
TRG_ENDOCYTIC_2	558	561	PF00928	0.366
TRG_ENDOCYTIC_2	88	91	PF00928	0.432
TRG_ENDOCYTIC_2	95	98	PF00928	0.275
TRG_ER_diArg_1	186	188	PF00400	0.443
TRG_ER_diArg_1	221	223	PF00400	0.417
TRG_ER_diArg_1	383	386	PF00400	0.646
TRG_ER_diArg_1	410	413	PF00400	0.498
TRG_ER_diArg_1	533	536	PF00400	0.420
TRG_ER_diArg_1	537	540	PF00400	0.419
TRG_Pf-PMV_PEXEL_1	234	238	PF00026	0.409
TRG_Pf-PMV_PEXEL_1	451	455	PF00026	0.495

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0V4	Leptomonas seymouri	48%	100%
A0A0S4J0D7	Bodo saltans	27%	100%
A0A1X0NK59	Trypanosomatidae	36%	100%
A0A3S7WPQ8	Leishmania donovani	90%	98%
A0A422NTD5	Trypanosoma rangeli	40%	100%
A4H4V6	Leishmania braziliensis	78%	99%
A4HT56	Leishmania infantum	90%	98%
C9ZUV8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
O13947	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
Q1QBM4	Psychrobacter cryohalolentis (strain ATCC BAA-1226 / DSM 17306 / VKM B-2378 / K5)	28%	100%
Q4FSB4	Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4)	28%	100%
Q4QIT1	Leishmania major	88%	100%
Q503J2	Danio rerio	25%	100%
Q5ZKW0	Gallus gallus	25%	100%
V5BDF2	Trypanosoma cruzi	39%	100%