Putative ubiquitin activating E1 enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Ubiquitin-like modifier-activating enzyme ATG7, putative

Species:

Leishmania mexicana

UniProt:

E9AL10_LEIMU

TriTrypDb:

LmxM.07.0010

Length:

725

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000407	phagophore assembly site	2	1
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AL10

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AL10

Function

Biological processes
Term	Name	Level	Count
GO:0000045	autophagosome assembly	6	1
GO:0000422	autophagy of mitochondrion	4	1
GO:0006497	protein lipidation	5	1
GO:0006501	C-terminal protein lipidation	6	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006914	autophagy	3	1
GO:0006950	response to stress	2	1
GO:0006995	cellular response to nitrogen starvation	5	1
GO:0006996	organelle organization	4	1
GO:0007005	mitochondrion organization	5	1
GO:0007033	vacuole organization	5	1
GO:0007154	cell communication	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009267	cellular response to starvation	4	1
GO:0009605	response to external stimulus	2	1
GO:0009987	cellular process	1	1
GO:0009991	response to extracellular stimulus	3	1
GO:0016043	cellular component organization	3	1
GO:0016237	lysosomal microautophagy	4	1
GO:0018410	C-terminal protein amino acid modification	6	1
GO:0019538	protein metabolic process	3	1
GO:0022411	cellular component disassembly	4	1
GO:0022607	cellular component assembly	4	1
GO:0031667	response to nutrient levels	4	1
GO:0031668	cellular response to extracellular stimulus	3	1
GO:0031669	cellular response to nutrient levels	4	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0033554	cellular response to stress	3	1
GO:0034727	piecemeal microautophagy of the nucleus	5	1
GO:0036211	protein modification process	4	1
GO:0042594	response to starvation	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043562	cellular response to nitrogen levels	5	1
GO:0043687	post-translational protein modification	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044804	autophagy of nucleus	4	1
GO:0044805	late nucleophagy	5	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0061726	mitochondrion disassembly	6	1
GO:0061919	process utilizing autophagic mechanism	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0070925	organelle assembly	5	1
GO:0071496	cellular response to external stimulus	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1903008	organelle disassembly	5	1
GO:1905037	autophagosome organization	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	11
GO:0016874	ligase activity	2	11
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0140657	ATP-dependent activity	1	11
GO:0019778	Atg12 activating enzyme activity	3	1
GO:0019779	Atg8 activating enzyme activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	135	139	PF00656	0.540
CLV_C14_Caspase3-7	152	156	PF00656	0.390
CLV_C14_Caspase3-7	243	247	PF00656	0.511
CLV_C14_Caspase3-7	254	258	PF00656	0.465
CLV_C14_Caspase3-7	445	449	PF00656	0.372
CLV_NRD_NRD_1	343	345	PF00675	0.247
CLV_PCSK_SKI1_1	111	115	PF00082	0.214
CLV_PCSK_SKI1_1	120	124	PF00082	0.194
CLV_PCSK_SKI1_1	28	32	PF00082	0.314
CLV_PCSK_SKI1_1	295	299	PF00082	0.300
CLV_PCSK_SKI1_1	391	395	PF00082	0.297
CLV_PCSK_SKI1_1	406	410	PF00082	0.174
CLV_PCSK_SKI1_1	414	418	PF00082	0.239
CLV_PCSK_SKI1_1	55	59	PF00082	0.303
CLV_PCSK_SKI1_1	702	706	PF00082	0.338
DEG_APCC_DBOX_1	110	118	PF00400	0.461
DEG_APCC_DBOX_1	367	375	PF00400	0.340
DEG_APCC_DBOX_1	635	643	PF00400	0.389
DEG_COP1_1	506	517	PF00400	0.258
DEG_SIAH_1	458	466	PF03145	0.300
DOC_CKS1_1	562	567	PF01111	0.258
DOC_CKS1_1	57	62	PF01111	0.335
DOC_CYCLIN_RxL_1	25	34	PF00134	0.449
DOC_CYCLIN_RxL_1	388	399	PF00134	0.262
DOC_CYCLIN_yClb5_NLxxxL_5	105	114	PF00134	0.418
DOC_CYCLIN_yCln2_LP_2	122	128	PF00134	0.461
DOC_MAPK_DCC_7	375	384	PF00069	0.239
DOC_MAPK_gen_1	344	353	PF00069	0.560
DOC_MAPK_gen_1	375	384	PF00069	0.255
DOC_MAPK_HePTP_8	117	129	PF00069	0.418
DOC_MAPK_MEF2A_6	120	129	PF00069	0.405
DOC_MAPK_MEF2A_6	213	221	PF00069	0.444
DOC_MAPK_MEF2A_6	244	252	PF00069	0.449
DOC_MAPK_MEF2A_6	295	303	PF00069	0.500
DOC_MAPK_MEF2A_6	375	384	PF00069	0.239
DOC_MAPK_MEF2A_6	464	472	PF00069	0.289
DOC_PP1_RVXF_1	298	304	PF00149	0.501
DOC_PP1_RVXF_1	326	332	PF00149	0.408
DOC_PP1_RVXF_1	421	427	PF00149	0.372
DOC_PP2B_LxvP_1	275	278	PF13499	0.481
DOC_PP2B_PxIxI_1	524	530	PF00149	0.300
DOC_PP4_FxxP_1	356	359	PF00568	0.442
DOC_PP4_FxxP_1	57	60	PF00568	0.418
DOC_PP4_FxxP_1	619	622	PF00568	0.307
DOC_USP7_MATH_1	166	170	PF00917	0.472
DOC_USP7_MATH_1	171	175	PF00917	0.489
DOC_USP7_MATH_1	258	262	PF00917	0.433
DOC_USP7_MATH_1	276	280	PF00917	0.388
DOC_USP7_MATH_1	287	291	PF00917	0.418
DOC_USP7_MATH_1	40	44	PF00917	0.365
DOC_USP7_MATH_1	430	434	PF00917	0.372
DOC_USP7_MATH_1	50	54	PF00917	0.388
DOC_USP7_MATH_1	577	581	PF00917	0.239
DOC_USP7_MATH_1	595	599	PF00917	0.239
DOC_WW_Pin1_4	183	188	PF00397	0.435
DOC_WW_Pin1_4	230	235	PF00397	0.437
DOC_WW_Pin1_4	521	526	PF00397	0.372
DOC_WW_Pin1_4	56	61	PF00397	0.426
DOC_WW_Pin1_4	561	566	PF00397	0.258
DOC_WW_Pin1_4	688	693	PF00397	0.426
LIG_14-3-3_CanoR_1	201	207	PF00244	0.501
LIG_14-3-3_CanoR_1	267	277	PF00244	0.447
LIG_14-3-3_CanoR_1	421	427	PF00244	0.240
LIG_14-3-3_CanoR_1	431	435	PF00244	0.243
LIG_14-3-3_CanoR_1	49	58	PF00244	0.484
LIG_14-3-3_CanoR_1	594	604	PF00244	0.317
LIG_Actin_WH2_2	276	291	PF00022	0.498
LIG_Actin_WH2_2	98	113	PF00022	0.418
LIG_BIR_II_1	1	5	PF00653	0.558
LIG_BIR_III_4	138	142	PF00653	0.418
LIG_BIR_III_4	257	261	PF00653	0.381
LIG_BIR_III_4	713	717	PF00653	0.458
LIG_BRCT_BRCA1_1	225	229	PF00533	0.449
LIG_BRCT_BRCA1_1	579	583	PF00533	0.372
LIG_BRCT_BRCA1_1	690	694	PF00533	0.321
LIG_BRCT_BRCA1_1	97	101	PF00533	0.418
LIG_Clathr_ClatBox_1	434	438	PF01394	0.372
LIG_deltaCOP1_diTrp_1	138	145	PF00928	0.410
LIG_EH_1	215	219	PF12763	0.405
LIG_EH_1	662	666	PF12763	0.435
LIG_FHA_1	202	208	PF00498	0.403
LIG_FHA_1	233	239	PF00498	0.418
LIG_FHA_1	280	286	PF00498	0.418
LIG_FHA_1	395	401	PF00498	0.327
LIG_FHA_1	522	528	PF00498	0.275
LIG_FHA_1	533	539	PF00498	0.198
LIG_FHA_1	576	582	PF00498	0.372
LIG_FHA_2	150	156	PF00498	0.431
LIG_FHA_2	168	174	PF00498	0.405
LIG_FHA_2	241	247	PF00498	0.455
LIG_FHA_2	415	421	PF00498	0.239
LIG_FHA_2	443	449	PF00498	0.362
LIG_FHA_2	9	15	PF00498	0.411
LIG_GBD_Chelix_1	310	318	PF00786	0.249
LIG_GBD_Chelix_1	600	608	PF00786	0.317
LIG_LIR_Apic_2	54	60	PF02991	0.418
LIG_LIR_Apic_2	611	616	PF02991	0.372
LIG_LIR_Apic_2	617	622	PF02991	0.434
LIG_LIR_Gen_1	11	19	PF02991	0.466
LIG_LIR_Gen_1	226	237	PF02991	0.446
LIG_LIR_Gen_1	296	306	PF02991	0.448
LIG_LIR_Gen_1	322	329	PF02991	0.547
LIG_LIR_Gen_1	425	434	PF02991	0.363
LIG_LIR_Gen_1	534	544	PF02991	0.239
LIG_LIR_Nem_3	11	15	PF02991	0.454
LIG_LIR_Nem_3	226	232	PF02991	0.435
LIG_LIR_Nem_3	296	301	PF02991	0.459
LIG_LIR_Nem_3	302	306	PF02991	0.459
LIG_LIR_Nem_3	322	326	PF02991	0.514
LIG_LIR_Nem_3	333	338	PF02991	0.449
LIG_LIR_Nem_3	425	429	PF02991	0.363
LIG_LIR_Nem_3	580	586	PF02991	0.372
LIG_PAM2_1	334	346	PF00658	0.449
LIG_Pex14_1	331	335	PF04695	0.500
LIG_Rb_pABgroove_1	247	255	PF01858	0.418
LIG_SH2_NCK_1	130	134	PF00017	0.418
LIG_SH2_SRC	153	156	PF00017	0.500
LIG_SH2_SRC	613	616	PF00017	0.372
LIG_SH2_STAT5	153	156	PF00017	0.452
LIG_SH2_STAT5	536	539	PF00017	0.256
LIG_SH2_STAT5	569	572	PF00017	0.244
LIG_SH3_3	184	190	PF00018	0.529
LIG_SH3_3	459	465	PF00018	0.368
LIG_SH3_3	589	595	PF00018	0.347
LIG_SH3_3	686	692	PF00018	0.333
LIG_SUMO_SIM_anti_2	415	420	PF11976	0.245
LIG_TRAF2_1	132	135	PF00917	0.498
LIG_TRAF2_1	305	308	PF00917	0.493
LIG_TRAF2_1	719	722	PF00917	0.514
LIG_TRAF2_2	359	364	PF00917	0.448
LIG_UBA3_1	114	120	PF00899	0.452
LIG_UBA3_1	150	157	PF00899	0.449
LIG_WRC_WIRS_1	229	234	PF05994	0.461
LIG_WRC_WIRS_1	434	439	PF05994	0.372
LIG_WRC_WIRS_1	96	101	PF05994	0.503
MOD_CK1_1	2	8	PF00069	0.563
MOD_CK1_1	279	285	PF00069	0.460
MOD_CK1_1	290	296	PF00069	0.459
MOD_CK1_1	309	315	PF00069	0.325
MOD_CK1_1	433	439	PF00069	0.372
MOD_CK1_1	599	605	PF00069	0.350
MOD_CK1_1	649	655	PF00069	0.373
MOD_CK1_1	67	73	PF00069	0.517
MOD_CK1_1	74	80	PF00069	0.522
MOD_CK2_1	129	135	PF00069	0.561
MOD_CK2_1	167	173	PF00069	0.385
MOD_CK2_1	310	316	PF00069	0.476
MOD_CK2_1	690	696	PF00069	0.311
MOD_CK2_1	715	721	PF00069	0.610
MOD_GlcNHglycan	1	4	PF01048	0.520
MOD_GlcNHglycan	131	134	PF01048	0.298
MOD_GlcNHglycan	173	176	PF01048	0.222
MOD_GlcNHglycan	191	194	PF01048	0.280
MOD_GlcNHglycan	289	292	PF01048	0.364
MOD_GlcNHglycan	340	343	PF01048	0.236
MOD_GlcNHglycan	459	462	PF01048	0.300
MOD_GlcNHglycan	601	604	PF01048	0.253
MOD_GlcNHglycan	616	619	PF01048	0.326
MOD_GlcNHglycan	66	69	PF01048	0.255
MOD_GSK3_1	167	174	PF00069	0.542
MOD_GSK3_1	228	235	PF00069	0.520
MOD_GSK3_1	236	243	PF00069	0.496
MOD_GSK3_1	306	313	PF00069	0.526
MOD_GSK3_1	334	341	PF00069	0.431
MOD_GSK3_1	575	582	PF00069	0.372
MOD_GSK3_1	595	602	PF00069	0.120
MOD_GSK3_1	67	74	PF00069	0.533
MOD_GSK3_1	712	719	PF00069	0.451
MOD_N-GLC_1	223	228	PF02516	0.249
MOD_N-GLC_1	50	55	PF02516	0.249
MOD_NEK2_1	310	315	PF00069	0.463
MOD_NEK2_1	338	343	PF00069	0.488
MOD_NEK2_1	394	399	PF00069	0.347
MOD_NEK2_1	651	656	PF00069	0.480
MOD_NEK2_1	712	717	PF00069	0.521
MOD_NEK2_2	430	435	PF00069	0.372
MOD_NEK2_2	558	563	PF00069	0.300
MOD_PIKK_1	74	80	PF00454	0.403
MOD_PK_1	244	250	PF00069	0.411
MOD_PKA_2	316	322	PF00069	0.544
MOD_PKA_2	422	428	PF00069	0.281
MOD_PKA_2	430	436	PF00069	0.203
MOD_PKA_2	558	564	PF00069	0.438
MOD_PKA_2	646	652	PF00069	0.308
MOD_Plk_1	223	229	PF00069	0.544
MOD_Plk_1	293	299	PF00069	0.418
MOD_Plk_1	306	312	PF00069	0.418
MOD_Plk_1	414	420	PF00069	0.326
MOD_Plk_4	113	119	PF00069	0.459
MOD_Plk_4	157	163	PF00069	0.477
MOD_Plk_4	202	208	PF00069	0.539
MOD_Plk_4	244	250	PF00069	0.484
MOD_Plk_4	293	299	PF00069	0.430
MOD_Plk_4	306	312	PF00069	0.418
MOD_Plk_4	414	420	PF00069	0.308
MOD_Plk_4	430	436	PF00069	0.187
MOD_Plk_4	513	519	PF00069	0.239
MOD_Plk_4	532	538	PF00069	0.239
MOD_Plk_4	646	652	PF00069	0.327
MOD_Plk_4	690	696	PF00069	0.372
MOD_ProDKin_1	183	189	PF00069	0.435
MOD_ProDKin_1	230	236	PF00069	0.437
MOD_ProDKin_1	521	527	PF00069	0.372
MOD_ProDKin_1	56	62	PF00069	0.426
MOD_ProDKin_1	561	567	PF00069	0.258
MOD_ProDKin_1	688	694	PF00069	0.424
MOD_SUMO_for_1	299	302	PF00179	0.335
MOD_SUMO_for_1	706	709	PF00179	0.424
MOD_SUMO_rev_2	438	445	PF00179	0.300
MOD_SUMO_rev_2	478	482	PF00179	0.166
TRG_DiLeu_BaEn_1	173	178	PF01217	0.449
TRG_DiLeu_BaEn_2	293	299	PF01217	0.500
TRG_DiLeu_BaEn_4	103	109	PF01217	0.449
TRG_DiLeu_BaEn_4	333	339	PF01217	0.449
TRG_ENDOCYTIC_2	159	162	PF00928	0.424
TRG_ENDOCYTIC_2	536	539	PF00928	0.239
TRG_ER_diArg_1	421	424	PF00400	0.300
TRG_ER_diArg_1	636	639	PF00400	0.375
TRG_NLS_MonoCore_2	343	348	PF00514	0.500
TRG_NLS_MonoExtC_3	343	349	PF00514	0.500
TRG_Pf-PMV_PEXEL_1	484	488	PF00026	0.300
TRG_Pf-PMV_PEXEL_1	584	588	PF00026	0.239

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2V7	Leptomonas seymouri	64%	100%
A0A1X0P0S1	Trypanosomatidae	37%	88%
A0A3R7K2D8	Trypanosoma rangeli	42%	95%
A0A3S5H5V4	Leishmania donovani	95%	100%
A3LPA1	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	30%	100%
A4H4U4	Leishmania braziliensis	83%	100%
A4HT22	Leishmania infantum	95%	100%
A5DLC6	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	31%	100%
A5E0T7	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	31%	100%
A6QXC6	Ajellomyces capsulatus (strain NAm1 / WU24)	32%	100%
A6ZT79	Saccharomyces cerevisiae (strain YJM789)	30%	100%
A7EI75	Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1)	30%	100%
A7KAI6	Pichia angusta	31%	100%
A7KAL8	Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)	37%	100%
A7TEY0	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	30%	100%
D0A4R0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	96%
F7W4M2	Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)	35%	100%
G2XR75	Botryotinia fuckeliana (strain T4)	30%	100%
I1S0J7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	34%	100%
M7U9B9	Botryotinia fuckeliana (strain BcDW1)	30%	95%
O43069	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
O93922	Komagataella pastoris	30%	100%
O95352	Homo sapiens	31%	100%
P0CM38	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	32%	100%
P0CM39	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	32%	100%
P38862	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	100%
Q4QIU4	Leishmania major	95%	100%
Q52CS0	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	30%	100%
Q5AWA2	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	34%	100%
Q5ZKY2	Gallus gallus	32%	100%
Q641Y5	Rattus norvegicus	32%	100%
Q6BGV9	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	31%	100%
Q6FQY7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	100%
Q94CD5	Arabidopsis thaliana	32%	100%
Q9D906	Mus musculus	32%	100%
V5B6Z1	Trypanosoma cruzi	41%	95%