Bifunctional dihydrofolate reductase-thymidylate synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dihydrofolate reductase-thymidylate synthase

Species:

Leishmania mexicana

UniProt:

E9AKW4_LEIMU

TriTrypDb:

LmxM.06.0860

Length:

520

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AKW4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AKW4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006220	pyrimidine nucleotide metabolic process	5	6
GO:0006221	pyrimidine nucleotide biosynthetic process	6	6
GO:0006231	dTMP biosynthetic process	8	6
GO:0006575	cellular modified amino acid metabolic process	3	6
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006730	one-carbon metabolic process	3	6
GO:0006753	nucleoside phosphate metabolic process	4	6
GO:0006760	folic acid-containing compound metabolic process	4	6
GO:0006793	phosphorus metabolic process	3	6
GO:0006796	phosphate-containing compound metabolic process	4	6
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008152	metabolic process	1	6
GO:0009058	biosynthetic process	2	6
GO:0009117	nucleotide metabolic process	5	6
GO:0009123	nucleoside monophosphate metabolic process	5	6
GO:0009124	nucleoside monophosphate biosynthetic process	6	6
GO:0009129	pyrimidine nucleoside monophosphate metabolic process	6	6
GO:0009130	pyrimidine nucleoside monophosphate biosynthetic process	7	6
GO:0009157	deoxyribonucleoside monophosphate biosynthetic process	7	6
GO:0009162	deoxyribonucleoside monophosphate metabolic process	6	6
GO:0009165	nucleotide biosynthetic process	6	6
GO:0009176	pyrimidine deoxyribonucleoside monophosphate metabolic process	7	6
GO:0009177	pyrimidine deoxyribonucleoside monophosphate biosynthetic process	8	6
GO:0009219	pyrimidine deoxyribonucleotide metabolic process	6	6
GO:0009221	pyrimidine deoxyribonucleotide biosynthetic process	7	6
GO:0009262	deoxyribonucleotide metabolic process	4	6
GO:0009263	deoxyribonucleotide biosynthetic process	5	6
GO:0009265	2'-deoxyribonucleotide biosynthetic process	6	6
GO:0009394	2'-deoxyribonucleotide metabolic process	5	6
GO:0009396	folic acid-containing compound biosynthetic process	5	6
GO:0009987	cellular process	1	6
GO:0018130	heterocycle biosynthetic process	4	6
GO:0019438	aromatic compound biosynthetic process	4	6
GO:0019637	organophosphate metabolic process	3	6
GO:0019692	deoxyribose phosphate metabolic process	4	6
GO:0032259	methylation	2	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0034654	nucleobase-containing compound biosynthetic process	4	6
GO:0042398	cellular modified amino acid biosynthetic process	4	6
GO:0042558	pteridine-containing compound metabolic process	4	6
GO:0042559	pteridine-containing compound biosynthetic process	5	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0044249	cellular biosynthetic process	3	6
GO:0044271	cellular nitrogen compound biosynthetic process	4	6
GO:0044281	small molecule metabolic process	2	6
GO:0046073	dTMP metabolic process	7	6
GO:0046385	deoxyribose phosphate biosynthetic process	5	6
GO:0046483	heterocycle metabolic process	3	6
GO:0046653	tetrahydrofolate metabolic process	5	6
GO:0046654	tetrahydrofolate biosynthetic process	6	6
GO:0055086	nucleobase-containing small molecule metabolic process	3	6
GO:0071704	organic substance metabolic process	2	6
GO:0072527	pyrimidine-containing compound metabolic process	4	6
GO:0072528	pyrimidine-containing compound biosynthetic process	5	6
GO:0090407	organophosphate biosynthetic process	4	6
GO:1901135	carbohydrate derivative metabolic process	3	6
GO:1901137	carbohydrate derivative biosynthetic process	4	6
GO:1901293	nucleoside phosphate biosynthetic process	5	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1901362	organic cyclic compound biosynthetic process	4	6
GO:1901564	organonitrogen compound metabolic process	3	6
GO:1901566	organonitrogen compound biosynthetic process	4	6
GO:1901576	organic substance biosynthetic process	3	6

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0004146	dihydrofolate reductase activity	5	6
GO:0004799	thymidylate synthase activity	6	6
GO:0008168	methyltransferase activity	4	6
GO:0016491	oxidoreductase activity	2	6
GO:0016645	oxidoreductase activity, acting on the CH-NH group of donors	3	6
GO:0016646	oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor	4	6
GO:0016740	transferase activity	2	6
GO:0016741	transferase activity, transferring one-carbon groups	3	6
GO:0042083	5,10-methylenetetrahydrofolate-dependent methyltransferase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	215	219	PF00656	0.426
CLV_C14_Caspase3-7	270	274	PF00656	0.491
CLV_C14_Caspase3-7	321	325	PF00656	0.491
CLV_C14_Caspase3-7	458	462	PF00656	0.491
CLV_NRD_NRD_1	212	214	PF00675	0.271
CLV_NRD_NRD_1	380	382	PF00675	0.291
CLV_NRD_NRD_1	80	82	PF00675	0.259
CLV_PCSK_KEX2_1	119	121	PF00082	0.259
CLV_PCSK_KEX2_1	212	214	PF00082	0.259
CLV_PCSK_KEX2_1	282	284	PF00082	0.291
CLV_PCSK_KEX2_1	380	382	PF00082	0.291
CLV_PCSK_KEX2_1	73	75	PF00082	0.291
CLV_PCSK_PC1ET2_1	119	121	PF00082	0.259
CLV_PCSK_PC1ET2_1	282	284	PF00082	0.291
CLV_PCSK_PC1ET2_1	73	75	PF00082	0.291
CLV_PCSK_SKI1_1	243	247	PF00082	0.317
CLV_PCSK_SKI1_1	282	286	PF00082	0.335
CLV_PCSK_SKI1_1	320	324	PF00082	0.311
CLV_PCSK_SKI1_1	326	330	PF00082	0.265
CLV_PCSK_SKI1_1	380	384	PF00082	0.291
CLV_PCSK_SKI1_1	394	398	PF00082	0.200
CLV_PCSK_SKI1_1	492	496	PF00082	0.259
CLV_PCSK_SKI1_1	92	96	PF00082	0.291
DEG_APCC_DBOX_1	234	242	PF00400	0.342
DOC_CYCLIN_yCln2_LP_2	428	434	PF00134	0.491
DOC_MAPK_FxFP_2	19	22	PF00069	0.398
DOC_MAPK_FxFP_2	193	196	PF00069	0.459
DOC_MAPK_gen_1	7	14	PF00069	0.325
DOC_MAPK_gen_1	81	89	PF00069	0.473
DOC_MAPK_gen_1	92	102	PF00069	0.438
DOC_MAPK_MEF2A_6	25	33	PF00069	0.342
DOC_MAPK_MEF2A_6	422	430	PF00069	0.491
DOC_MAPK_MEF2A_6	82	91	PF00069	0.470
DOC_MAPK_MEF2A_6	95	102	PF00069	0.428
DOC_PP1_RVXF_1	280	287	PF00149	0.491
DOC_PP1_RVXF_1	378	385	PF00149	0.426
DOC_PP2B_LxvP_1	111	114	PF13499	0.459
DOC_PP2B_LxvP_1	428	431	PF13499	0.491
DOC_PP4_FxxP_1	19	22	PF00568	0.299
DOC_PP4_FxxP_1	193	196	PF00568	0.459
DOC_PP4_FxxP_1	8	11	PF00568	0.416
DOC_USP7_MATH_1	145	149	PF00917	0.459
DOC_USP7_MATH_1	20	24	PF00917	0.386
DOC_WW_Pin1_4	165	170	PF00397	0.495
LIG_14-3-3_CanoR_1	181	187	PF00244	0.491
LIG_14-3-3_CanoR_1	212	220	PF00244	0.491
LIG_14-3-3_CanoR_1	268	272	PF00244	0.491
LIG_14-3-3_CanoR_1	326	334	PF00244	0.497
LIG_14-3-3_CanoR_1	381	389	PF00244	0.491
LIG_14-3-3_CanoR_1	81	85	PF00244	0.491
LIG_Actin_WH2_2	435	453	PF00022	0.491
LIG_BIR_II_1	1	5	PF00653	0.556
LIG_BRCT_BRCA1_1	318	322	PF00533	0.491
LIG_Clathr_ClatBox_1	500	504	PF01394	0.378
LIG_CtBP_PxDLS_1	50	54	PF00389	0.491
LIG_EVH1_1	193	197	PF00568	0.491
LIG_FHA_1	257	263	PF00498	0.410
LIG_FHA_1	279	285	PF00498	0.491
LIG_FHA_1	439	445	PF00498	0.459
LIG_FHA_1	58	64	PF00498	0.491
LIG_FHA_2	105	111	PF00498	0.491
LIG_FHA_2	13	19	PF00498	0.446
LIG_FHA_2	200	206	PF00498	0.463
LIG_FHA_2	330	336	PF00498	0.430
LIG_FHA_2	456	462	PF00498	0.491
LIG_LIR_Apic_2	18	22	PF02991	0.414
LIG_LIR_Apic_2	190	196	PF02991	0.548
LIG_LIR_Apic_2	44	50	PF02991	0.491
LIG_LIR_Gen_1	234	245	PF02991	0.342
LIG_LIR_Gen_1	436	445	PF02991	0.491
LIG_LIR_Gen_1	496	506	PF02991	0.378
LIG_LIR_Gen_1	83	91	PF02991	0.491
LIG_LIR_Nem_3	18	24	PF02991	0.410
LIG_LIR_Nem_3	190	195	PF02991	0.503
LIG_LIR_Nem_3	234	240	PF02991	0.342
LIG_LIR_Nem_3	350	354	PF02991	0.474
LIG_LIR_Nem_3	436	440	PF02991	0.491
LIG_LIR_Nem_3	83	87	PF02991	0.491
LIG_REV1ctd_RIR_1	53	61	PF16727	0.491
LIG_SH2_CRK	437	441	PF00017	0.491
LIG_SH2_GRB2like	407	410	PF00017	0.491
LIG_SH2_SRC	162	165	PF00017	0.459
LIG_SH2_SRC	508	511	PF00017	0.459
LIG_SH2_STAP1	162	166	PF00017	0.491
LIG_SH2_STAT3	340	343	PF00017	0.491
LIG_SH2_STAT5	146	149	PF00017	0.478
LIG_SH2_STAT5	154	157	PF00017	0.452
LIG_SH2_STAT5	178	181	PF00017	0.491
LIG_SH2_STAT5	227	230	PF00017	0.393
LIG_SH2_STAT5	237	240	PF00017	0.426
LIG_SH2_STAT5	407	410	PF00017	0.491
LIG_SH2_STAT5	508	511	PF00017	0.436
LIG_SH3_1	191	197	PF00018	0.491
LIG_SH3_3	191	197	PF00018	0.517
LIG_SH3_3	506	512	PF00018	0.489
LIG_SUMO_SIM_anti_2	126	131	PF11976	0.491
LIG_SUMO_SIM_par_1	28	34	PF11976	0.491
LIG_TRAF2_1	107	110	PF00917	0.459
LIG_UBA3_1	111	119	PF00899	0.297
LIG_UBA3_1	240	246	PF00899	0.342
LIG_UBA3_1	439	446	PF00899	0.342
MOD_CK1_1	104	110	PF00069	0.342
MOD_CK1_1	149	155	PF00069	0.342
MOD_CK1_1	413	419	PF00069	0.297
MOD_CK2_1	104	110	PF00069	0.342
MOD_CK2_1	145	151	PF00069	0.342
MOD_Cter_Amidation	210	213	PF01082	0.297
MOD_Cter_Amidation	79	82	PF01082	0.297
MOD_GlcNHglycan	214	217	PF01048	0.192
MOD_GSK3_1	100	107	PF00069	0.342
MOD_GSK3_1	145	152	PF00069	0.342
MOD_GSK3_1	177	184	PF00069	0.291
MOD_GSK3_1	252	259	PF00069	0.348
MOD_GSK3_1	322	329	PF00069	0.364
MOD_N-GLC_1	316	321	PF02516	0.342
MOD_NEK2_1	206	211	PF00069	0.342
MOD_NEK2_1	267	272	PF00069	0.460
MOD_NEK2_1	278	283	PF00069	0.306
MOD_NEK2_1	322	327	PF00069	0.342
MOD_NEK2_1	382	387	PF00069	0.342
MOD_NEK2_1	455	460	PF00069	0.342
MOD_NEK2_2	258	263	PF00069	0.342
MOD_OFUCOSY	417	424	PF10250	0.342
MOD_PIKK_1	206	212	PF00454	0.342
MOD_PK_1	25	31	PF00069	0.342
MOD_PKA_1	212	218	PF00069	0.297
MOD_PKA_2	212	218	PF00069	0.297
MOD_PKA_2	267	273	PF00069	0.342
MOD_PKA_2	80	86	PF00069	0.342
MOD_Plk_1	413	419	PF00069	0.342
MOD_Plk_1	57	63	PF00069	0.329
MOD_Plk_4	258	264	PF00069	0.229
MOD_Plk_4	438	444	PF00069	0.342
MOD_Plk_4	482	488	PF00069	0.342
MOD_Plk_4	58	64	PF00069	0.326
MOD_ProDKin_1	165	171	PF00069	0.348
MOD_SUMO_for_1	488	491	PF00179	0.342
TRG_DiLeu_BaEn_1	496	501	PF01217	0.184
TRG_DiLeu_BaEn_2	489	495	PF01217	0.252
TRG_DiLeu_BaEn_3	291	297	PF01217	0.342
TRG_DiLeu_BaEn_4	233	239	PF01217	0.342
TRG_DiLeu_BaEn_4	490	496	PF01217	0.297
TRG_DiLeu_BaLyEn_6	136	141	PF01217	0.184
TRG_DiLeu_BaLyEn_6	274	279	PF01217	0.342
TRG_DiLeu_BaLyEn_6	378	383	PF01217	0.342
TRG_ENDOCYTIC_2	237	240	PF00928	0.342
TRG_ENDOCYTIC_2	351	354	PF00928	0.350
TRG_ENDOCYTIC_2	437	440	PF00928	0.342
TRG_ENDOCYTIC_2	498	501	PF00928	0.273
TRG_ER_diArg_1	380	382	PF00400	0.342
TRG_Pf-PMV_PEXEL_1	326	330	PF00026	0.297

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJX9	Leptomonas seymouri	78%	98%
A0A0S4JJI0	Bodo saltans	61%	100%
A0A1X0NKM2	Trypanosomatidae	68%	100%
A0A3R7P0P8	Trypanosoma rangeli	67%	100%
A0A3S5H5T0	Leishmania donovani	94%	100%
A4H4P8	Leishmania braziliensis	87%	100%
C9ZTK8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	99%
O81395	Zea mays	47%	100%
P07382	Leishmania major	93%	100%
P16126	Leishmania amazonensis	99%	100%
P20712	Plasmodium chabaudi	38%	89%
P45350	Daucus carota	45%	98%
P51820	Glycine max	45%	98%
Q05762	Arabidopsis thaliana	46%	100%
Q05763	Arabidopsis thaliana	46%	92%
Q23695	Crithidia fasciculata	81%	100%
Q27783	Trypanosoma brucei brucei	61%	99%
Q27793	Trypanosoma cruzi	68%	100%
Q27828	Paramecium tetraurelia	43%	100%
Q2QRX6	Oryza sativa subsp. japonica	47%	100%
Q8MQV3	Leishmania infantum	94%	100%
V5DPL1	Trypanosoma cruzi	68%	100%