Putative glutamine synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glutamine synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9AKR5_LEIMU

TriTrypDb:

LmxM.06.0370

Length:

379

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 4
Pissara et al.	no	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AKR5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AKR5

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	13
GO:0006520	amino acid metabolic process	3	13
GO:0006541	glutamine metabolic process	6	13
GO:0006542	glutamine biosynthetic process	7	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0008652	amino acid biosynthetic process	4	13
GO:0009058	biosynthetic process	2	13
GO:0009064	glutamine family amino acid metabolic process	5	13
GO:0009084	glutamine family amino acid biosynthetic process	6	13
GO:0009987	cellular process	1	13
GO:0016053	organic acid biosynthetic process	4	13
GO:0019752	carboxylic acid metabolic process	5	13
GO:0043436	oxoacid metabolic process	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044249	cellular biosynthetic process	3	13
GO:0044281	small molecule metabolic process	2	13
GO:0044283	small molecule biosynthetic process	3	13
GO:0046394	carboxylic acid biosynthetic process	5	13
GO:0071704	organic substance metabolic process	2	13
GO:1901564	organonitrogen compound metabolic process	3	13
GO:1901566	organonitrogen compound biosynthetic process	4	13
GO:1901576	organic substance biosynthetic process	3	13
GO:1901605	alpha-amino acid metabolic process	4	13
GO:1901607	alpha-amino acid biosynthetic process	5	13

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0004356	glutamate-ammonia ligase activity	6	13
GO:0016211	ammonia ligase activity	5	13
GO:0016874	ligase activity	2	13
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	13
GO:0016880	acid-ammonia (or amide) ligase activity	4	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	17	21	PF00656	0.517
CLV_C14_Caspase3-7	231	235	PF00656	0.564
CLV_NRD_NRD_1	171	173	PF00675	0.307
CLV_NRD_NRD_1	335	337	PF00675	0.302
CLV_PCSK_KEX2_1	293	295	PF00082	0.371
CLV_PCSK_KEX2_1	335	337	PF00082	0.289
CLV_PCSK_PC1ET2_1	293	295	PF00082	0.195
CLV_PCSK_SKI1_1	221	225	PF00082	0.297
CLV_PCSK_SKI1_1	293	297	PF00082	0.362
CLV_PCSK_SKI1_1	375	379	PF00082	0.625
CLV_PCSK_SKI1_1	44	48	PF00082	0.306
DEG_APCC_DBOX_1	220	228	PF00400	0.506
DEG_SPOP_SBC_1	85	89	PF00917	0.547
DOC_CKS1_1	357	362	PF01111	0.528
DOC_CYCLIN_yCln2_LP_2	73	79	PF00134	0.530
DOC_MAPK_DCC_7	86	96	PF00069	0.506
DOC_MAPK_gen_1	26	34	PF00069	0.517
DOC_PP1_RVXF_1	280	287	PF00149	0.530
DOC_PP2B_PxIxI_1	91	97	PF00149	0.506
DOC_PP4_FxxP_1	153	156	PF00568	0.515
DOC_USP7_MATH_1	323	327	PF00917	0.591
DOC_USP7_MATH_1	85	89	PF00917	0.546
DOC_USP7_UBL2_3	278	282	PF12436	0.564
DOC_WW_Pin1_4	194	199	PF00397	0.569
DOC_WW_Pin1_4	356	361	PF00397	0.492
DOC_WW_Pin1_4	363	368	PF00397	0.456
LIG_14-3-3_CanoR_1	10	14	PF00244	0.497
LIG_14-3-3_CanoR_1	113	120	PF00244	0.524
LIG_14-3-3_CanoR_1	275	281	PF00244	0.530
LIG_14-3-3_CanoR_1	314	321	PF00244	0.546
LIG_14-3-3_CanoR_1	349	357	PF00244	0.497
LIG_14-3-3_CanoR_1	86	94	PF00244	0.483
LIG_BIR_II_1	1	5	PF00653	0.588
LIG_BIR_III_2	340	344	PF00653	0.517
LIG_BRCT_BRCA1_1	347	351	PF00533	0.517
LIG_deltaCOP1_diTrp_1	370	378	PF00928	0.473
LIG_FHA_1	29	35	PF00498	0.497
LIG_FHA_1	299	305	PF00498	0.536
LIG_FHA_1	345	351	PF00498	0.562
LIG_FHA_1	357	363	PF00498	0.341
LIG_FHA_1	6	12	PF00498	0.538
LIG_FHA_1	68	74	PF00498	0.510
LIG_FHA_2	113	119	PF00498	0.516
LIG_FHA_2	190	196	PF00498	0.506
LIG_FHA_2	258	264	PF00498	0.517
LIG_IRF3_LxIS_1	183	190	PF10401	0.564
LIG_LIR_Gen_1	12	20	PF02991	0.496
LIG_LIR_Nem_3	12	16	PF02991	0.496
LIG_LIR_Nem_3	126	132	PF02991	0.435
LIG_NRBOX	223	229	PF00104	0.588
LIG_PCNA_yPIPBox_3	216	226	PF02747	0.506
LIG_Pex14_1	170	174	PF04695	0.603
LIG_PTB_Apo_2	46	53	PF02174	0.603
LIG_SH2_PTP2	13	16	PF00017	0.497
LIG_SH2_SRC	101	104	PF00017	0.581
LIG_SH2_STAP1	179	183	PF00017	0.493
LIG_SH2_STAT3	33	36	PF00017	0.588
LIG_SH2_STAT5	101	104	PF00017	0.564
LIG_SH2_STAT5	13	16	PF00017	0.497
LIG_SH2_STAT5	136	139	PF00017	0.534
LIG_SH2_STAT5	161	164	PF00017	0.496
LIG_SH2_STAT5	272	275	PF00017	0.497
LIG_SH2_STAT5	33	36	PF00017	0.497
LIG_SH2_STAT5	344	347	PF00017	0.497
LIG_SUMO_SIM_par_1	93	99	PF11976	0.506
LIG_SxIP_EBH_1	86	97	PF03271	0.588
LIG_TYR_ITIM	11	16	PF00017	0.506
MOD_CK1_1	112	118	PF00069	0.526
MOD_CK1_1	163	169	PF00069	0.588
MOD_CK1_1	256	262	PF00069	0.546
MOD_CK1_1	302	308	PF00069	0.588
MOD_CK2_1	112	118	PF00069	0.518
MOD_CK2_1	189	195	PF00069	0.506
MOD_CK2_1	257	263	PF00069	0.508
MOD_Cter_Amidation	170	173	PF01082	0.306
MOD_GlcNHglycan	103	106	PF01048	0.494
MOD_GlcNHglycan	16	19	PF01048	0.326
MOD_GlcNHglycan	167	170	PF01048	0.401
MOD_GlcNHglycan	189	192	PF01048	0.306
MOD_GlcNHglycan	248	251	PF01048	0.297
MOD_GlcNHglycan	306	310	PF01048	0.357
MOD_GSK3_1	1	8	PF00069	0.563
MOD_GSK3_1	101	108	PF00069	0.567
MOD_GSK3_1	112	119	PF00069	0.561
MOD_GSK3_1	14	21	PF00069	0.444
MOD_GSK3_1	161	168	PF00069	0.579
MOD_GSK3_1	24	31	PF00069	0.487
MOD_GSK3_1	246	253	PF00069	0.497
MOD_GSK3_1	274	281	PF00069	0.580
MOD_GSK3_1	295	302	PF00069	0.538
MOD_GSK3_1	310	317	PF00069	0.440
MOD_N-GLC_1	187	192	PF02516	0.317
MOD_N-GLC_1	246	251	PF02516	0.291
MOD_N-GLC_1	253	258	PF02516	0.293
MOD_N-GLC_1	5	10	PF02516	0.531
MOD_NEK2_1	1	6	PF00069	0.653
MOD_NEK2_1	187	192	PF00069	0.518
MOD_NEK2_1	276	281	PF00069	0.488
MOD_NEK2_1	345	350	PF00069	0.506
MOD_NEK2_1	57	62	PF00069	0.493
MOD_PIKK_1	32	38	PF00454	0.603
MOD_PK_1	314	320	PF00069	0.497
MOD_PKA_1	293	299	PF00069	0.395
MOD_PKA_2	112	118	PF00069	0.539
MOD_PKA_2	274	280	PF00069	0.583
MOD_PKA_2	293	299	PF00069	0.391
MOD_PKA_2	313	319	PF00069	0.367
MOD_PKA_2	85	91	PF00069	0.506
MOD_PKA_2	9	15	PF00069	0.348
MOD_Plk_1	28	34	PF00069	0.497
MOD_Plk_1	5	11	PF00069	0.523
MOD_Plk_2-3	358	364	PF00069	0.600
MOD_Plk_4	9	15	PF00069	0.348
MOD_ProDKin_1	194	200	PF00069	0.569
MOD_ProDKin_1	356	362	PF00069	0.486
MOD_ProDKin_1	363	369	PF00069	0.453
TRG_DiLeu_BaEn_2	132	138	PF01217	0.506
TRG_DiLeu_BaLyEn_6	223	228	PF01217	0.586
TRG_ENDOCYTIC_2	13	16	PF00928	0.496
TRG_ENDOCYTIC_2	136	139	PF00928	0.536
TRG_ER_diArg_1	335	337	PF00400	0.497
TRG_Pf-PMV_PEXEL_1	226	230	PF00026	0.297

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6V5	Leptomonas seymouri	84%	100%
A0A0S4IX33	Bodo saltans	62%	100%
A0A1X0NKP6	Trypanosomatidae	76%	100%
A0A1X0NL55	Trypanosomatidae	76%	99%
A0A3R7R8D5	Trypanosoma rangeli	76%	99%
A0A3S5H5P8	Leishmania donovani	97%	100%
A4H4K2	Leishmania braziliensis	92%	100%
A4HSS8	Leishmania infantum	97%	78%
C9ZTD4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	90%
O00088	Agaricus bisporus	44%	100%
O04867	Alnus glutinosa	44%	100%
O22504	Daucus carota	44%	100%
O22506	Daucus carota	46%	88%
O82560	Glycine max	47%	100%
P00965	Phaseolus vulgaris	45%	100%
P04078	Medicago sativa	44%	100%
P04770	Phaseolus vulgaris	44%	100%
P04771	Phaseolus vulgaris	44%	100%
P04772	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	39%	100%
P04773	Cricetulus griseus	43%	100%
P07694	Pisum sativum	44%	100%
P08281	Pisum sativum	44%	88%
P08282	Pisum sativum	44%	100%
P09606	Rattus norvegicus	45%	100%
P0CN84	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	45%	100%
P0CN85	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	45%	100%
P10656	Clostridium saccharobutylicum	26%	86%
P12424	Nicotiana plumbaginifolia	44%	100%
P13564	Hordeum vulgare	46%	87%
P14636	Lupinus angustifolius	46%	100%
P14654	Oryza sativa subsp. japonica	46%	100%
P14655	Oryza sativa subsp. japonica	45%	89%
P14656	Oryza sativa subsp. japonica	44%	100%
P15102	Phaseolus vulgaris	46%	88%
P15103	Bos taurus	45%	100%
P15104	Homo sapiens	46%	100%
P15105	Mus musculus	45%	100%
P16580	Gallus gallus	45%	100%
P19432	Streptomyces viridochromogenes	43%	100%
P20477	Drosophila melanogaster	41%	95%
P20478	Drosophila melanogaster	46%	100%
P20805	Frankia alni	40%	100%
P22878	Streptomyces hygroscopicus	40%	100%
P23712	Lactuca sativa	45%	100%
P24099	Glycine max	44%	100%
P25462	Zea mays	45%	90%
P32288	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	47%	100%
P32289	Vigna aconitifolia	44%	100%
P34497	Caenorhabditis elegans	45%	100%
P38559	Zea mays	45%	100%
P38560	Zea mays	44%	100%
P38561	Zea mays	45%	100%
P38562	Zea mays	45%	100%
P38563	Zea mays	45%	100%
P41320	Squalus acanthias	44%	94%
P45626	Rhizobium meliloti	39%	100%
P46410	Sus scrofa	45%	100%
P51118	Vitis vinifera	45%	100%
P51119	Vitis vinifera	45%	100%
P51121	Xenopus laevis	47%	97%
P52782	Lupinus luteus	46%	100%
P52783	Pinus sylvestris	44%	100%
P64246	Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)	24%	85%
P9WN36	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	24%	85%
P9WN37	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	24%	85%
Q02154	Rhizobium leguminosarum bv. phaseoli	39%	100%
Q04831	Panulirus argus	46%	100%
Q06378	Hordeum vulgare	43%	100%
Q09179	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	45%	100%
Q12613	Colletotrichum gloeosporioides	48%	100%
Q42624	Brassica napus	47%	89%
Q42688	Chlamydomonas reinhardtii	43%	99%
Q42689	Chlamydomonas reinhardtii	42%	100%
Q42899	Lotus japonicus	44%	100%
Q43066	Pisum sativum	44%	100%
Q43127	Arabidopsis thaliana	47%	88%
Q43785	Medicago sativa	43%	100%
Q4QJ42	Leishmania major	97%	100%
Q4R7U3	Macaca fascicularis	46%	100%
Q4W8D0	Oryza sativa subsp. japonica	44%	100%
Q56WN1	Arabidopsis thaliana	44%	100%
Q5SIP0	Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)	26%	85%
Q5UR44	Acanthamoeba polyphaga mimivirus	46%	100%
Q60182	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	25%	83%
Q6B4U7	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	43%	100%
Q6C3E0	Yarrowia lipolytica (strain CLIB 122 / E 150)	45%	100%
Q6FMT6	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	45%	100%
Q75BT9	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	48%	100%
Q86ZF9	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	49%	100%
Q86ZU6	Tuber borchii	46%	100%
Q874T6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	47%	100%
Q8GXW5	Arabidopsis thaliana	45%	100%
Q8HZM5	Canis lupus familiaris	45%	100%
Q8J1R3	Suillus bovinus	43%	100%
Q8LCE1	Arabidopsis thaliana	44%	100%
Q8X169	Amanita muscaria	44%	100%
Q96UV5	Hebeloma cylindrosporum	46%	100%
Q96V52	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	46%	100%
Q9C2U9	Gibberella fujikuroi	47%	100%
Q9FMD9	Arabidopsis thaliana	44%	100%
Q9LVI8	Arabidopsis thaliana	45%	100%
Q9QY94	Acomys cahirinus	45%	100%
Q9UUN6	Fusarium solani subsp. phaseoli	48%	100%
Q9XQ94	Medicago sativa	45%	89%
V5CHQ8	Trypanosoma cruzi	75%	99%