Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania mexicana

UniProt:

E9AKJ9_LEIMU

TriTrypDb:

LmxM.05.0930

Length:

463

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0016020	membrane	2	2
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AKJ9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AKJ9

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016874	ligase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	454	456	PF00675	0.362
CLV_PCSK_KEX2_1	454	456	PF00082	0.362
CLV_PCSK_KEX2_1	9	11	PF00082	0.265
CLV_PCSK_PC1ET2_1	9	11	PF00082	0.310
DEG_APCC_DBOX_1	453	461	PF00400	0.362
DEG_SCF_FBW7_1	217	223	PF00400	0.645
DEG_SCF_FBW7_1	361	368	PF00400	0.613
DEG_SPOP_SBC_1	227	231	PF00917	0.620
DOC_CKS1_1	217	222	PF01111	0.621
DOC_CKS1_1	319	324	PF01111	0.742
DOC_CKS1_1	362	367	PF01111	0.616
DOC_CYCLIN_yCln2_LP_2	237	243	PF00134	0.666
DOC_MAPK_MEF2A_6	420	427	PF00069	0.569
DOC_MAPK_RevD_3	441	455	PF00069	0.362
DOC_PP2B_LxvP_1	187	190	PF13499	0.804
DOC_PP2B_LxvP_1	73	76	PF13499	0.510
DOC_SPAK_OSR1_1	86	90	PF12202	0.465
DOC_USP7_MATH_1	243	247	PF00917	0.675
DOC_USP7_MATH_1	398	402	PF00917	0.656
DOC_USP7_MATH_1	4	8	PF00917	0.478
DOC_WW_Pin1_4	174	179	PF00397	0.644
DOC_WW_Pin1_4	216	221	PF00397	0.740
DOC_WW_Pin1_4	223	228	PF00397	0.685
DOC_WW_Pin1_4	236	241	PF00397	0.636
DOC_WW_Pin1_4	265	270	PF00397	0.732
DOC_WW_Pin1_4	275	280	PF00397	0.662
DOC_WW_Pin1_4	286	291	PF00397	0.634
DOC_WW_Pin1_4	296	301	PF00397	0.671
DOC_WW_Pin1_4	307	312	PF00397	0.725
DOC_WW_Pin1_4	318	323	PF00397	0.670
DOC_WW_Pin1_4	354	359	PF00397	0.718
DOC_WW_Pin1_4	361	366	PF00397	0.703
DOC_WW_Pin1_4	378	383	PF00397	0.617
DOC_WW_Pin1_4	400	405	PF00397	0.701
LIG_Actin_WH2_2	440	456	PF00022	0.308
LIG_APCC_ABBA_1	170	175	PF00400	0.603
LIG_BIR_II_1	1	5	PF00653	0.562
LIG_BIR_III_2	379	383	PF00653	0.715
LIG_BRCT_BRCA1_1	83	87	PF00533	0.465
LIG_deltaCOP1_diTrp_1	144	153	PF00928	0.569
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.465
LIG_EVH1_1	187	191	PF00568	0.790
LIG_FHA_1	106	112	PF00498	0.510
LIG_FHA_1	160	166	PF00498	0.564
LIG_FHA_1	310	316	PF00498	0.735
LIG_FHA_1	379	385	PF00498	0.712
LIG_FHA_1	387	393	PF00498	0.607
LIG_LIR_Apic_2	27	32	PF02991	0.465
LIG_LIR_Nem_3	12	18	PF02991	0.510
LIG_LYPXL_S_1	172	176	PF13949	0.296
LIG_LYPXL_yS_3	173	176	PF13949	0.490
LIG_MYND_1	185	189	PF01753	0.650
LIG_MYND_1	269	273	PF01753	0.619
LIG_MYND_1	282	286	PF01753	0.602
LIG_NRBOX	442	448	PF00104	0.238
LIG_NRBOX	456	462	PF00104	0.430
LIG_Pex14_1	105	109	PF04695	0.465
LIG_Pex14_2	38	42	PF04695	0.465
LIG_PROFILIN_1	188	194	PF00235	0.656
LIG_PTAP_UEV_1	272	277	PF05743	0.618
LIG_REV1ctd_RIR_1	78	87	PF16727	0.510
LIG_SH2_CRK	15	19	PF00017	0.538
LIG_SH2_PTP2	29	32	PF00017	0.510
LIG_SH2_SRC	109	112	PF00017	0.530
LIG_SH2_STAP1	109	113	PF00017	0.465
LIG_SH2_STAT5	29	32	PF00017	0.510
LIG_SH3_1	186	192	PF00018	0.669
LIG_SH3_1	69	75	PF00018	0.465
LIG_SH3_3	182	188	PF00018	0.685
LIG_SH3_3	204	210	PF00018	0.706
LIG_SH3_3	214	220	PF00018	0.706
LIG_SH3_3	221	227	PF00018	0.720
LIG_SH3_3	266	272	PF00018	0.636
LIG_SH3_3	273	279	PF00018	0.665
LIG_SH3_3	280	286	PF00018	0.615
LIG_SH3_3	287	293	PF00018	0.612
LIG_SH3_3	316	322	PF00018	0.691
LIG_SH3_3	359	365	PF00018	0.768
LIG_SH3_3	367	373	PF00018	0.748
LIG_SH3_3	401	407	PF00018	0.665
LIG_SH3_3	409	415	PF00018	0.662
LIG_SH3_3	427	433	PF00018	0.581
LIG_SH3_3	69	75	PF00018	0.465
LIG_SUMO_SIM_par_1	136	142	PF11976	0.545
LIG_TRAF2_1	336	339	PF00917	0.631
LIG_TRFH_1	71	75	PF08558	0.510
LIG_UBA3_1	137	143	PF00899	0.534
LIG_WRC_WIRS_1	442	447	PF05994	0.362
MOD_CDC14_SPxK_1	177	180	PF00782	0.629
MOD_CDC14_SPxK_1	357	360	PF00782	0.704
MOD_CDK_SPK_2	286	291	PF00069	0.628
MOD_CDK_SPxK_1	174	180	PF00069	0.620
MOD_CDK_SPxK_1	354	360	PF00069	0.704
MOD_CK1_1	2	8	PF00069	0.576
MOD_CK1_1	223	229	PF00069	0.708
MOD_CK1_1	231	237	PF00069	0.771
MOD_CK1_1	262	268	PF00069	0.675
MOD_CK1_1	274	280	PF00069	0.631
MOD_CK1_1	386	392	PF00069	0.609
MOD_CK1_1	403	409	PF00069	0.760
MOD_CK1_1	95	101	PF00069	0.481
MOD_CK2_1	333	339	PF00069	0.778
MOD_GlcNHglycan	201	204	PF01048	0.581
MOD_GlcNHglycan	273	276	PF01048	0.443
MOD_GlcNHglycan	305	308	PF01048	0.453
MOD_GlcNHglycan	323	326	PF01048	0.496
MOD_GlcNHglycan	385	388	PF01048	0.573
MOD_GlcNHglycan	400	403	PF01048	0.449
MOD_GlcNHglycan	83	86	PF01048	0.286
MOD_GSK3_1	147	154	PF00069	0.546
MOD_GSK3_1	216	223	PF00069	0.715
MOD_GSK3_1	226	233	PF00069	0.740
MOD_GSK3_1	271	278	PF00069	0.635
MOD_GSK3_1	294	301	PF00069	0.654
MOD_GSK3_1	303	310	PF00069	0.697
MOD_GSK3_1	361	368	PF00069	0.636
MOD_GSK3_1	378	385	PF00069	0.713
MOD_GSK3_1	92	99	PF00069	0.498
MOD_NEK2_1	113	118	PF00069	0.510
MOD_NEK2_1	153	158	PF00069	0.575
MOD_NEK2_1	383	388	PF00069	0.617
MOD_NEK2_1	428	433	PF00069	0.726
MOD_NEK2_1	65	70	PF00069	0.585
MOD_NEK2_1	81	86	PF00069	0.418
MOD_NEK2_2	4	9	PF00069	0.457
MOD_NEK2_2	40	45	PF00069	0.510
MOD_PIKK_1	262	268	PF00454	0.642
MOD_PKA_1	9	15	PF00069	0.510
MOD_PKA_2	9	15	PF00069	0.522
MOD_Plk_1	428	434	PF00069	0.660
MOD_Plk_1	50	56	PF00069	0.552
MOD_Plk_4	232	238	PF00069	0.701
MOD_Plk_4	388	394	PF00069	0.659
MOD_Plk_4	441	447	PF00069	0.303
MOD_ProDKin_1	174	180	PF00069	0.645
MOD_ProDKin_1	216	222	PF00069	0.743
MOD_ProDKin_1	223	229	PF00069	0.687
MOD_ProDKin_1	236	242	PF00069	0.638
MOD_ProDKin_1	265	271	PF00069	0.732
MOD_ProDKin_1	275	281	PF00069	0.661
MOD_ProDKin_1	286	292	PF00069	0.632
MOD_ProDKin_1	296	302	PF00069	0.670
MOD_ProDKin_1	307	313	PF00069	0.724
MOD_ProDKin_1	318	324	PF00069	0.671
MOD_ProDKin_1	354	360	PF00069	0.719
MOD_ProDKin_1	361	367	PF00069	0.705
MOD_ProDKin_1	378	384	PF00069	0.615
MOD_ProDKin_1	400	406	PF00069	0.700
MOD_SUMO_rev_2	164	172	PF00179	0.478
MOD_SUMO_rev_2	208	212	PF00179	0.664
TRG_ENDOCYTIC_2	15	18	PF00928	0.510
TRG_ENDOCYTIC_2	173	176	PF00928	0.490
TRG_ER_diArg_1	453	455	PF00400	0.362

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3S5H5K9	Leishmania donovani	68%	100%
A4HSL5	Leishmania infantum	68%	100%
Q4QJA9	Leishmania major	69%	98%
V5B702	Trypanosoma cruzi	39%	100%