tRNA 4-demethylwyosine synthase (AdoMet-dependent)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Flavodoxin/Radical SAM superfamily/Wyosine base formation, putative

Species:

Leishmania mexicana

UniProt:

E9AKI1_LEIMU

TriTrypDb:

LmxM.05.0750

Length:

798

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0016020	membrane	2	3

Expansion

Sequence features

E9AKI1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AKI1

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006396	RNA processing	6	10
GO:0006399	tRNA metabolic process	7	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008033	tRNA processing	8	10
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034470	ncRNA processing	7	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0034660	ncRNA metabolic process	6	10
GO:0043170	macromolecule metabolic process	3	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10
GO:0006400	tRNA modification	6	1
GO:0009058	biosynthetic process	2	1
GO:0009451	RNA modification	5	1
GO:0031590	wybutosine metabolic process	4	1
GO:0031591	wybutosine biosynthetic process	5	1
GO:0043412	macromolecule modification	4	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901137	carbohydrate derivative biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901657	glycosyl compound metabolic process	4	1
GO:1901659	glycosyl compound biosynthetic process	5	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	10
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	10
GO:0010181	FMN binding	4	10
GO:0016829	lyase activity	2	10
GO:0032553	ribonucleotide binding	3	10
GO:0036094	small molecule binding	2	10
GO:0043167	ion binding	2	10
GO:0043168	anion binding	3	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0051536	iron-sulfur cluster binding	3	10
GO:0051539	4 iron, 4 sulfur cluster binding	4	10
GO:0051540	metal cluster binding	2	10
GO:0097159	organic cyclic compound binding	2	10
GO:0097367	carbohydrate derivative binding	2	10
GO:1901265	nucleoside phosphate binding	3	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	160	164	PF00656	0.432
CLV_C14_Caspase3-7	338	342	PF00656	0.761
CLV_NRD_NRD_1	192	194	PF00675	0.261
CLV_NRD_NRD_1	601	603	PF00675	0.181
CLV_NRD_NRD_1	785	787	PF00675	0.377
CLV_PCSK_KEX2_1	579	581	PF00082	0.224
CLV_PCSK_PC1ET2_1	579	581	PF00082	0.224
CLV_PCSK_SKI1_1	178	182	PF00082	0.232
CLV_PCSK_SKI1_1	259	263	PF00082	0.191
CLV_PCSK_SKI1_1	422	426	PF00082	0.244
CLV_PCSK_SKI1_1	456	460	PF00082	0.206
CLV_PCSK_SKI1_1	507	511	PF00082	0.230
CLV_PCSK_SKI1_1	558	562	PF00082	0.209
CLV_PCSK_SKI1_1	583	587	PF00082	0.206
CLV_PCSK_SKI1_1	606	610	PF00082	0.206
CLV_PCSK_SKI1_1	640	644	PF00082	0.206
CLV_PCSK_SKI1_1	728	732	PF00082	0.245
DEG_APCC_DBOX_1	53	61	PF00400	0.589
DEG_MDM2_SWIB_1	586	594	PF02201	0.406
DEG_MDM2_SWIB_1	731	739	PF02201	0.515
DEG_Nend_Nbox_1	1	3	PF02207	0.321
DOC_CKS1_1	683	688	PF01111	0.474
DOC_CYCLIN_yCln2_LP_2	12	18	PF00134	0.271
DOC_MAPK_DCC_7	473	483	PF00069	0.449
DOC_MAPK_gen_1	558	568	PF00069	0.403
DOC_MAPK_gen_1	602	613	PF00069	0.409
DOC_MAPK_MEF2A_6	475	483	PF00069	0.449
DOC_MAPK_MEF2A_6	558	566	PF00069	0.413
DOC_PIKK_1	362	370	PF02985	0.632
DOC_PP2B_LxvP_1	12	15	PF13499	0.271
DOC_PP2B_LxvP_1	225	228	PF13499	0.489
DOC_PP4_FxxP_1	230	233	PF00568	0.488
DOC_USP7_MATH_1	282	286	PF00917	0.721
DOC_USP7_MATH_1	345	349	PF00917	0.726
DOC_USP7_MATH_1	693	697	PF00917	0.331
DOC_USP7_MATH_1	87	91	PF00917	0.478
DOC_WW_Pin1_4	277	282	PF00397	0.677
DOC_WW_Pin1_4	294	299	PF00397	0.670
DOC_WW_Pin1_4	445	450	PF00397	0.426
DOC_WW_Pin1_4	501	506	PF00397	0.406
DOC_WW_Pin1_4	571	576	PF00397	0.394
DOC_WW_Pin1_4	682	687	PF00397	0.474
DOC_WW_Pin1_4	761	766	PF00397	0.458
LIG_14-3-3_CanoR_1	188	192	PF00244	0.489
LIG_14-3-3_CanoR_1	247	257	PF00244	0.489
LIG_14-3-3_CanoR_1	312	317	PF00244	0.531
LIG_14-3-3_CanoR_1	419	425	PF00244	0.533
LIG_14-3-3_CanoR_1	507	514	PF00244	0.465
LIG_14-3-3_CanoR_1	542	547	PF00244	0.477
LIG_14-3-3_CanoR_1	592	600	PF00244	0.475
LIG_14-3-3_CanoR_1	610	614	PF00244	0.332
LIG_14-3-3_CanoR_1	790	795	PF00244	0.664
LIG_Actin_WH2_2	563	581	PF00022	0.424
LIG_BRCT_BRCA1_1	169	173	PF00533	0.414
LIG_BRCT_BRCA1_1	257	261	PF00533	0.381
LIG_BRCT_BRCA1_1	468	472	PF00533	0.424
LIG_Clathr_ClatBox_1	723	727	PF01394	0.489
LIG_deltaCOP1_diTrp_1	732	738	PF00928	0.515
LIG_FHA_1	103	109	PF00498	0.512
LIG_FHA_1	152	158	PF00498	0.457
LIG_FHA_1	160	166	PF00498	0.505
LIG_FHA_1	240	246	PF00498	0.491
LIG_FHA_1	249	255	PF00498	0.438
LIG_FHA_1	265	271	PF00498	0.504
LIG_FHA_1	295	301	PF00498	0.620
LIG_FHA_1	446	452	PF00498	0.406
LIG_FHA_1	463	469	PF00498	0.406
LIG_FHA_1	617	623	PF00498	0.406
LIG_FHA_1	780	786	PF00498	0.614
LIG_FHA_2	113	119	PF00498	0.584
LIG_FHA_2	142	148	PF00498	0.501
LIG_FHA_2	158	164	PF00498	0.372
LIG_FHA_2	336	342	PF00498	0.634
LIG_FHA_2	572	578	PF00498	0.406
LIG_FHA_2	735	741	PF00498	0.440
LIG_FHA_2	754	760	PF00498	0.389
LIG_GBD_Chelix_1	17	25	PF00786	0.359
LIG_LIR_Gen_1	170	181	PF02991	0.406
LIG_LIR_Gen_1	208	218	PF02991	0.426
LIG_LIR_Gen_1	635	644	PF02991	0.416
LIG_LIR_Gen_1	740	748	PF02991	0.448
LIG_LIR_Gen_1	753	763	PF02991	0.460
LIG_LIR_Nem_3	208	214	PF02991	0.426
LIG_LIR_Nem_3	244	249	PF02991	0.478
LIG_LIR_Nem_3	391	397	PF02991	0.489
LIG_LIR_Nem_3	435	439	PF02991	0.431
LIG_LIR_Nem_3	635	639	PF02991	0.416
LIG_LIR_Nem_3	737	741	PF02991	0.427
LIG_LIR_Nem_3	742	748	PF02991	0.449
LIG_LIR_Nem_3	753	758	PF02991	0.470
LIG_LIR_Nem_3	759	763	PF02991	0.457
LIG_LIR_Nem_3	764	770	PF02991	0.420
LIG_LYPXL_S_1	393	397	PF13949	0.292
LIG_LYPXL_S_1	527	531	PF13949	0.206
LIG_LYPXL_yS_3	394	397	PF13949	0.499
LIG_LYPXL_yS_3	528	531	PF13949	0.406
LIG_MAD2	783	791	PF02301	0.593
LIG_Pex14_2	586	590	PF04695	0.406
LIG_Pex14_2	731	735	PF04695	0.526
LIG_PTB_Apo_2	547	554	PF02174	0.406
LIG_SH2_CRK	406	410	PF00017	0.456
LIG_SH2_CRK	491	495	PF00017	0.489
LIG_SH2_CRK	683	687	PF00017	0.489
LIG_SH2_CRK	760	764	PF00017	0.450
LIG_SH2_NCK_1	745	749	PF00017	0.495
LIG_SH2_NCK_1	760	764	PF00017	0.416
LIG_SH2_STAP1	151	155	PF00017	0.449
LIG_SH2_STAP1	22	26	PF00017	0.583
LIG_SH2_STAP1	236	240	PF00017	0.519
LIG_SH2_STAP1	625	629	PF00017	0.406
LIG_SH2_STAP1	741	745	PF00017	0.523
LIG_SH2_STAT3	86	89	PF00017	0.455
LIG_SH2_STAT5	161	164	PF00017	0.410
LIG_SH2_STAT5	567	570	PF00017	0.406
LIG_SH2_STAT5	644	647	PF00017	0.406
LIG_SH3_3	116	122	PF00018	0.527
LIG_SH3_3	162	168	PF00018	0.461
LIG_SH3_3	228	234	PF00018	0.476
LIG_SH3_3	519	525	PF00018	0.406
LIG_SH3_3	68	74	PF00018	0.553
LIG_SH3_3	760	766	PF00018	0.416
LIG_SH3_4	561	568	PF00018	0.387
LIG_SUMO_SIM_anti_2	696	702	PF11976	0.478
LIG_SUMO_SIM_par_1	722	727	PF11976	0.489
LIG_TRAF2_1	574	577	PF00917	0.424
LIG_TRAF2_1	702	705	PF00917	0.510
LIG_TRAF2_2	554	559	PF00917	0.422
LIG_TYR_ITIM	20	25	PF00017	0.511
LIG_TYR_ITIM	392	397	PF00017	0.487
LIG_TYR_ITIM	526	531	PF00017	0.406
LIG_TYR_ITIM	758	763	PF00017	0.543
LIG_UBA3_1	723	728	PF00899	0.504
LIG_WRC_WIRS_1	543	548	PF05994	0.428
LIG_WW_1	233	236	PF00397	0.473
LIG_WW_3	166	170	PF00397	0.489
MOD_CDK_SPxK_1	501	507	PF00069	0.406
MOD_CK1_1	102	108	PF00069	0.497
MOD_CK1_1	159	165	PF00069	0.433
MOD_CK1_1	28	34	PF00069	0.594
MOD_CK1_1	648	654	PF00069	0.406
MOD_CK1_1	750	756	PF00069	0.497
MOD_CK1_1	90	96	PF00069	0.507
MOD_CK2_1	141	147	PF00069	0.509
MOD_CK2_1	282	288	PF00069	0.703
MOD_CK2_1	571	577	PF00069	0.406
MOD_CMANNOS	735	738	PF00535	0.219
MOD_Cter_Amidation	191	194	PF01082	0.226
MOD_GlcNHglycan	284	287	PF01048	0.480
MOD_GlcNHglycan	300	303	PF01048	0.392
MOD_GlcNHglycan	321	324	PF01048	0.557
MOD_GlcNHglycan	328	333	PF01048	0.492
MOD_GlcNHglycan	412	415	PF01048	0.229
MOD_GlcNHglycan	63	66	PF01048	0.447
MOD_GlcNHglycan	67	70	PF01048	0.419
MOD_GlcNHglycan	749	752	PF01048	0.284
MOD_GSK3_1	235	242	PF00069	0.489
MOD_GSK3_1	284	291	PF00069	0.698
MOD_GSK3_1	294	301	PF00069	0.755
MOD_GSK3_1	43	50	PF00069	0.673
MOD_GSK3_1	462	469	PF00069	0.424
MOD_GSK3_1	542	549	PF00069	0.406
MOD_GSK3_1	61	68	PF00069	0.657
MOD_GSK3_1	640	647	PF00069	0.406
MOD_GSK3_1	750	757	PF00069	0.557
MOD_GSK3_1	790	797	PF00069	0.606
MOD_N-GLC_1	439	444	PF02516	0.317
MOD_N-GLC_2	138	140	PF02516	0.374
MOD_N-GLC_2	455	457	PF02516	0.206
MOD_N-GLC_2	667	669	PF02516	0.131
MOD_NEK2_1	156	161	PF00069	0.444
MOD_NEK2_1	20	25	PF00069	0.406
MOD_NEK2_1	318	323	PF00069	0.724
MOD_NEK2_1	408	413	PF00069	0.441
MOD_NEK2_1	546	551	PF00069	0.406
MOD_NEK2_1	609	614	PF00069	0.406
MOD_NEK2_1	747	752	PF00069	0.472
MOD_NEK2_1	8	13	PF00069	0.359
MOD_NEK2_2	151	156	PF00069	0.437
MOD_NEK2_2	266	271	PF00069	0.575
MOD_NEK2_2	432	437	PF00069	0.428
MOD_OFUCOSY	714	720	PF10250	0.206
MOD_PIKK_1	167	173	PF00454	0.424
MOD_PIKK_1	239	245	PF00454	0.536
MOD_PIKK_1	312	318	PF00454	0.689
MOD_PIKK_1	439	445	PF00454	0.516
MOD_PIKK_1	85	91	PF00454	0.466
MOD_PKA_1	271	277	PF00069	0.610
MOD_PKA_2	187	193	PF00069	0.449
MOD_PKA_2	311	317	PF00069	0.531
MOD_PKA_2	418	424	PF00069	0.466
MOD_PKA_2	541	547	PF00069	0.426
MOD_PKA_2	609	615	PF00069	0.406
MOD_PKA_2	789	795	PF00069	0.603
MOD_Plk_1	753	759	PF00069	0.552
MOD_Plk_1	779	785	PF00069	0.561
MOD_Plk_2-3	754	760	PF00069	0.542
MOD_Plk_4	141	147	PF00069	0.464
MOD_Plk_4	151	157	PF00069	0.435
MOD_Plk_4	241	247	PF00069	0.498
MOD_Plk_4	420	426	PF00069	0.505
MOD_Plk_4	432	438	PF00069	0.365
MOD_Plk_4	517	523	PF00069	0.406
MOD_Plk_4	542	548	PF00069	0.428
MOD_Plk_4	609	615	PF00069	0.406
MOD_Plk_4	734	740	PF00069	0.427
MOD_Plk_4	790	796	PF00069	0.618
MOD_ProDKin_1	277	283	PF00069	0.680
MOD_ProDKin_1	294	300	PF00069	0.669
MOD_ProDKin_1	445	451	PF00069	0.406
MOD_ProDKin_1	501	507	PF00069	0.406
MOD_ProDKin_1	571	577	PF00069	0.394
MOD_ProDKin_1	682	688	PF00069	0.474
MOD_ProDKin_1	761	767	PF00069	0.457
MOD_SUMO_rev_2	203	211	PF00179	0.331
MOD_SUMO_rev_2	555	563	PF00179	0.394
MOD_SUMO_rev_2	571	581	PF00179	0.417
MOD_SUMO_rev_2	635	642	PF00179	0.406
TRG_DiLeu_BaEn_1	388	393	PF01217	0.509
TRG_ENDOCYTIC_2	22	25	PF00928	0.653
TRG_ENDOCYTIC_2	394	397	PF00928	0.499
TRG_ENDOCYTIC_2	406	409	PF00928	0.456
TRG_ENDOCYTIC_2	491	494	PF00928	0.489
TRG_ENDOCYTIC_2	528	531	PF00928	0.406
TRG_ENDOCYTIC_2	567	570	PF00928	0.406
TRG_ENDOCYTIC_2	625	628	PF00928	0.406
TRG_ENDOCYTIC_2	741	744	PF00928	0.443
TRG_ENDOCYTIC_2	745	748	PF00928	0.449
TRG_ENDOCYTIC_2	760	763	PF00928	0.455
TRG_ER_diArg_1	425	428	PF00400	0.443
TRG_ER_diArg_1	706	709	PF00400	0.461
TRG_NES_CRM1_1	559	571	PF08389	0.422
TRG_Pf-PMV_PEXEL_1	216	220	PF00026	0.289
TRG_Pf-PMV_PEXEL_1	583	587	PF00026	0.206

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I710	Leptomonas seymouri	67%	90%
A0A3S5H5J8	Leishmania donovani	89%	97%
A0A3S5IRA8	Trypanosoma rangeli	56%	100%
A4H4B8	Leishmania braziliensis	79%	99%
A4HSJ7	Leishmania infantum	89%	97%
C9ZU99	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	96%
Q2KHP8	Xenopus laevis	41%	100%
Q4QJC7	Leishmania major	89%	100%
Q8BJM7	Mus musculus	42%	100%
V5AVD9	Trypanosoma cruzi	54%	97%