Protein kinase domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein kinase, putative

Species:

Leishmania mexicana

UniProt:

E9AKE5_LEIMU

TriTrypDb:

LmxM.05.0390

Length:

733

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. A subfamily has 2TM regions, but the majority is cytoplasmic.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	2
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AKE5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AKE5

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	16
GO:0006793	phosphorus metabolic process	3	16
GO:0006796	phosphate-containing compound metabolic process	4	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0009987	cellular process	1	16
GO:0016310	phosphorylation	5	16
GO:0019538	protein metabolic process	3	16
GO:0036211	protein modification process	4	16
GO:0043170	macromolecule metabolic process	3	16
GO:0043412	macromolecule modification	4	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0071704	organic substance metabolic process	2	16
GO:1901564	organonitrogen compound metabolic process	3	16
GO:0007165	signal transduction	2	1
GO:0018105	peptidyl-serine phosphorylation	6	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018209	peptidyl-serine modification	6	1
GO:0035556	intracellular signal transduction	3	1
GO:0046777	protein autophosphorylation	6	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	16
GO:0003824	catalytic activity	1	16
GO:0004672	protein kinase activity	3	16
GO:0005488	binding	1	16
GO:0005524	ATP binding	5	16
GO:0016301	kinase activity	4	16
GO:0016740	transferase activity	2	16
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	16
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	16
GO:0017076	purine nucleotide binding	4	16
GO:0030554	adenyl nucleotide binding	5	16
GO:0032553	ribonucleotide binding	3	16
GO:0032555	purine ribonucleotide binding	4	16
GO:0032559	adenyl ribonucleotide binding	5	16
GO:0035639	purine ribonucleoside triphosphate binding	4	16
GO:0036094	small molecule binding	2	16
GO:0043167	ion binding	2	16
GO:0043168	anion binding	3	16
GO:0097159	organic cyclic compound binding	2	16
GO:0097367	carbohydrate derivative binding	2	16
GO:0140096	catalytic activity, acting on a protein	2	16
GO:1901265	nucleoside phosphate binding	3	16
GO:1901363	heterocyclic compound binding	2	16
GO:0004674	protein serine/threonine kinase activity	4	4
GO:0004683	calmodulin-dependent protein kinase activity	5	1
GO:0005515	protein binding	2	1
GO:0005516	calmodulin binding	3	1
GO:0009931	calcium-dependent protein serine/threonine kinase activity	5	1
GO:0010857	calcium-dependent protein kinase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	168	172	PF00656	0.304
CLV_C14_Caspase3-7	189	193	PF00656	0.465
CLV_C14_Caspase3-7	260	264	PF00656	0.427
CLV_C14_Caspase3-7	321	325	PF00656	0.497
CLV_C14_Caspase3-7	338	342	PF00656	0.482
CLV_C14_Caspase3-7	93	97	PF00656	0.618
CLV_NRD_NRD_1	382	384	PF00675	0.667
CLV_NRD_NRD_1	409	411	PF00675	0.703
CLV_NRD_NRD_1	506	508	PF00675	0.427
CLV_NRD_NRD_1	731	733	PF00675	0.669
CLV_PCSK_KEX2_1	382	384	PF00082	0.667
CLV_PCSK_KEX2_1	506	508	PF00082	0.323
CLV_PCSK_KEX2_1	731	733	PF00082	0.615
CLV_PCSK_SKI1_1	191	195	PF00082	0.331
CLV_PCSK_SKI1_1	577	581	PF00082	0.323
DEG_SCF_FBW7_1	334	340	PF00400	0.588
DOC_CKS1_1	334	339	PF01111	0.588
DOC_CKS1_1	617	622	PF01111	0.276
DOC_CYCLIN_yCln2_LP_2	235	241	PF00134	0.456
DOC_CYCLIN_yCln2_LP_2	711	717	PF00134	0.334
DOC_MAPK_gen_1	497	504	PF00069	0.276
DOC_MAPK_gen_1	523	530	PF00069	0.257
DOC_MAPK_gen_1	577	586	PF00069	0.323
DOC_MAPK_HePTP_8	520	532	PF00069	0.370
DOC_MAPK_MEF2A_6	523	532	PF00069	0.276
DOC_MAPK_MEF2A_6	580	588	PF00069	0.323
DOC_PP2B_LxvP_1	439	442	PF13499	0.643
DOC_USP7_MATH_1	21	25	PF00917	0.706
DOC_USP7_MATH_1	337	341	PF00917	0.603
DOC_USP7_MATH_1	401	405	PF00917	0.714
DOC_USP7_MATH_1	41	45	PF00917	0.786
DOC_USP7_UBL2_3	295	299	PF12436	0.309
DOC_USP7_UBL2_3	458	462	PF12436	0.323
DOC_WW_Pin1_4	333	338	PF00397	0.581
DOC_WW_Pin1_4	395	400	PF00397	0.745
DOC_WW_Pin1_4	430	435	PF00397	0.547
DOC_WW_Pin1_4	452	457	PF00397	0.531
DOC_WW_Pin1_4	48	53	PF00397	0.509
DOC_WW_Pin1_4	616	621	PF00397	0.316
DOC_WW_Pin1_4	722	727	PF00397	0.539
DOC_WW_Pin1_4	8	13	PF00397	0.693
LIG_14-3-3_CanoR_1	329	335	PF00244	0.479
LIG_14-3-3_CanoR_1	406	410	PF00244	0.596
LIG_14-3-3_CanoR_1	45	50	PF00244	0.770
LIG_14-3-3_CanoR_1	474	480	PF00244	0.433
LIG_14-3-3_CanoR_1	59	65	PF00244	0.755
LIG_Actin_WH2_2	461	476	PF00022	0.422
LIG_APCC_ABBA_1	291	296	PF00400	0.422
LIG_APCC_ABBA_1	528	533	PF00400	0.276
LIG_BIR_II_1	1	5	PF00653	0.513
LIG_BIR_III_4	192	196	PF00653	0.334
LIG_BIR_III_4	341	345	PF00653	0.602
LIG_BRCT_BRCA1_1	431	435	PF00533	0.480
LIG_BRCT_BRCA1_1	50	54	PF00533	0.468
LIG_BRCT_BRCA1_1	711	715	PF00533	0.293
LIG_Clathr_ClatBox_1	585	589	PF01394	0.276
LIG_deltaCOP1_diTrp_1	305	312	PF00928	0.279
LIG_eIF4E_1	109	115	PF01652	0.649
LIG_FHA_1	468	474	PF00498	0.437
LIG_FHA_1	525	531	PF00498	0.287
LIG_FHA_1	539	545	PF00498	0.276
LIG_FHA_1	718	724	PF00498	0.545
LIG_FHA_2	100	106	PF00498	0.736
LIG_FHA_2	129	135	PF00498	0.365
LIG_FHA_2	166	172	PF00498	0.316
LIG_FHA_2	258	264	PF00498	0.316
LIG_FHA_2	425	431	PF00498	0.686
LIG_FHA_2	645	651	PF00498	0.284
LIG_FHA_2	704	710	PF00498	0.417
LIG_FHA_2	87	93	PF00498	0.637
LIG_Integrin_isoDGR_2	277	279	PF01839	0.422
LIG_Integrin_isoDGR_2	475	477	PF01839	0.370
LIG_LIR_Apic_2	48	52	PF02991	0.476
LIG_LIR_Apic_2	619	625	PF02991	0.323
LIG_LIR_Gen_1	414	423	PF02991	0.568
LIG_LIR_Gen_1	663	672	PF02991	0.276
LIG_LIR_Nem_3	108	112	PF02991	0.661
LIG_LIR_Nem_3	414	420	PF02991	0.697
LIG_LIR_Nem_3	452	457	PF02991	0.397
LIG_LIR_Nem_3	464	468	PF02991	0.259
LIG_LIR_Nem_3	663	668	PF02991	0.276
LIG_MYND_1	701	705	PF01753	0.422
LIG_NRBOX	688	694	PF00104	0.422
LIG_Pex14_1	665	669	PF04695	0.276
LIG_Pex14_2	543	547	PF04695	0.276
LIG_SH2_CRK	109	113	PF00017	0.659
LIG_SH2_CRK	469	473	PF00017	0.378
LIG_SH2_CRK	560	564	PF00017	0.334
LIG_SH2_STAP1	469	473	PF00017	0.283
LIG_SH2_STAT5	109	112	PF00017	0.619
LIG_SH2_STAT5	128	131	PF00017	0.278
LIG_SH2_STAT5	167	170	PF00017	0.347
LIG_SH2_STAT5	179	182	PF00017	0.278
LIG_SH2_STAT5	197	200	PF00017	0.232
LIG_SH2_STAT5	282	285	PF00017	0.290
LIG_SH2_STAT5	290	293	PF00017	0.290
LIG_SH2_STAT5	469	472	PF00017	0.422
LIG_SH2_STAT5	534	537	PF00017	0.441
LIG_SH2_STAT5	568	571	PF00017	0.323
LIG_SH3_3	100	106	PF00018	0.584
LIG_SH3_3	331	337	PF00018	0.558
LIG_SH3_3	369	375	PF00018	0.684
LIG_SH3_3	385	391	PF00018	0.764
LIG_SH3_3	614	620	PF00018	0.276
LIG_SH3_3	653	659	PF00018	0.279
LIG_SH3_3	720	726	PF00018	0.517
LIG_SH3_3	76	82	PF00018	0.671
LIG_SUMO_SIM_anti_2	501	506	PF11976	0.276
LIG_SUMO_SIM_par_1	584	589	PF11976	0.290
LIG_TRAF2_1	345	348	PF00917	0.608
LIG_TRAF2_1	491	494	PF00917	0.422
LIG_TYR_ITIM	107	112	PF00017	0.656
LIG_UBA3_1	689	695	PF00899	0.391
LIG_WRC_WIRS_1	540	545	PF05994	0.290
MOD_CDK_SPxK_1	452	458	PF00069	0.533
MOD_CDK_SPxxK_3	430	437	PF00069	0.477
MOD_CK1_1	2	8	PF00069	0.741
MOD_CK1_1	31	37	PF00069	0.643
MOD_CK1_1	325	331	PF00069	0.531
MOD_CK1_1	365	371	PF00069	0.712
MOD_CK1_1	404	410	PF00069	0.615
MOD_CK1_1	412	418	PF00069	0.708
MOD_CK1_1	48	54	PF00069	0.634
MOD_CK1_1	536	542	PF00069	0.284
MOD_CK1_1	80	86	PF00069	0.644
MOD_CK2_1	128	134	PF00069	0.290
MOD_CK2_1	446	452	PF00069	0.531
MOD_CK2_1	488	494	PF00069	0.295
MOD_CK2_1	50	56	PF00069	0.531
MOD_CK2_1	644	650	PF00069	0.276
MOD_CK2_1	703	709	PF00069	0.464
MOD_CK2_1	722	728	PF00069	0.756
MOD_CK2_1	99	105	PF00069	0.515
MOD_GlcNHglycan	12	15	PF01048	0.747
MOD_GlcNHglycan	147	150	PF01048	0.361
MOD_GlcNHglycan	231	234	PF01048	0.428
MOD_GlcNHglycan	30	33	PF01048	0.643
MOD_GlcNHglycan	535	538	PF01048	0.276
MOD_GlcNHglycan	607	610	PF01048	0.422
MOD_GlcNHglycan	61	64	PF01048	0.577
MOD_GSK3_1	191	198	PF00069	0.300
MOD_GSK3_1	27	34	PF00069	0.803
MOD_GSK3_1	333	340	PF00069	0.721
MOD_GSK3_1	358	365	PF00069	0.733
MOD_GSK3_1	37	44	PF00069	0.765
MOD_GSK3_1	391	398	PF00069	0.658
MOD_GSK3_1	401	408	PF00069	0.632
MOD_GSK3_1	452	459	PF00069	0.616
MOD_GSK3_1	59	66	PF00069	0.482
MOD_GSK3_1	607	614	PF00069	0.336
MOD_GSK3_1	636	643	PF00069	0.276
MOD_GSK3_1	672	679	PF00069	0.277
MOD_GSK3_1	717	724	PF00069	0.566
MOD_N-GLC_1	395	400	PF02516	0.624
MOD_N-GLC_1	672	677	PF02516	0.370
MOD_N-GLC_1	8	13	PF02516	0.689
MOD_NEK2_1	1	6	PF00069	0.736
MOD_NEK2_1	364	369	PF00069	0.708
MOD_NEK2_1	405	410	PF00069	0.617
MOD_NEK2_1	435	440	PF00069	0.562
MOD_NEK2_1	58	63	PF00069	0.546
MOD_NEK2_1	597	602	PF00069	0.276
MOD_NEK2_1	727	732	PF00069	0.762
MOD_NEK2_2	401	406	PF00069	0.739
MOD_NEK2_2	551	556	PF00069	0.422
MOD_PIKK_1	322	328	PF00454	0.580
MOD_PK_1	546	552	PF00069	0.422
MOD_PKA_1	410	416	PF00069	0.574
MOD_PKA_2	278	284	PF00069	0.422
MOD_PKA_2	358	364	PF00069	0.707
MOD_PKA_2	405	411	PF00069	0.727
MOD_PKA_2	58	64	PF00069	0.578
MOD_PKA_2	703	709	PF00069	0.339
MOD_Plk_1	418	424	PF00069	0.511
MOD_Plk_2-3	343	349	PF00069	0.624
MOD_Plk_4	278	284	PF00069	0.318
MOD_Plk_4	330	336	PF00069	0.693
MOD_Plk_4	539	545	PF00069	0.290
MOD_Plk_4	612	618	PF00069	0.314
MOD_Plk_4	644	650	PF00069	0.276
MOD_Plk_4	660	666	PF00069	0.276
MOD_Plk_4	80	86	PF00069	0.635
MOD_ProDKin_1	333	339	PF00069	0.584
MOD_ProDKin_1	395	401	PF00069	0.746
MOD_ProDKin_1	430	436	PF00069	0.545
MOD_ProDKin_1	452	458	PF00069	0.533
MOD_ProDKin_1	48	54	PF00069	0.510
MOD_ProDKin_1	616	622	PF00069	0.316
MOD_ProDKin_1	722	728	PF00069	0.543
MOD_ProDKin_1	8	14	PF00069	0.695
MOD_SUMO_for_1	628	631	PF00179	0.290
MOD_SUMO_rev_2	185	193	PF00179	0.428
MOD_SUMO_rev_2	519	524	PF00179	0.403
TRG_DiLeu_BaEn_1	688	693	PF01217	0.439
TRG_DiLeu_BaEn_4	493	499	PF01217	0.334
TRG_ENDOCYTIC_2	109	112	PF00928	0.661
TRG_ENDOCYTIC_2	209	212	PF00928	0.403
TRG_ENDOCYTIC_2	290	293	PF00928	0.422
TRG_ENDOCYTIC_2	417	420	PF00928	0.703
TRG_ENDOCYTIC_2	469	472	PF00928	0.294
TRG_ENDOCYTIC_2	517	520	PF00928	0.441
TRG_ENDOCYTIC_2	560	563	PF00928	0.334
TRG_ER_diArg_1	180	183	PF00400	0.334
TRG_ER_diArg_1	381	383	PF00400	0.653
TRG_ER_diArg_1	505	507	PF00400	0.427
TRG_Pf-PMV_PEXEL_1	382	386	PF00026	0.549
TRG_Pf-PMV_PEXEL_1	567	571	PF00026	0.334

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZR8	Leptomonas seymouri	71%	92%
A0A0S4J7Q1	Bodo saltans	54%	100%
A0A1X0P0R3	Trypanosomatidae	64%	100%
A0A3S7WP16	Leishmania donovani	93%	93%
A0A3S7X8Z8	Leishmania donovani	26%	100%
A0A422N0Q9	Trypanosoma rangeli	60%	100%
A4H485	Leishmania braziliensis	82%	100%
A4HSG1	Leishmania infantum	93%	93%
A4IB02	Leishmania infantum	26%	100%
D0A4H1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9AET0	Leishmania major	27%	100%
E9B5Y5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
Q4QJG3	Leishmania major	93%	100%
V5D179	Trypanosoma cruzi	62%	100%