Probable eukaryotic initiation factor 4A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent RNA helicase, putative

Species:

Leishmania mexicana

UniProt:

E9AKE2_LEIMU

TriTrypDb:

LmxM.05.0360

Length:

621

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AKE2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AKE2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003724	RNA helicase activity	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0008186	ATP-dependent activity, acting on RNA	2	12
GO:0016787	hydrolase activity	2	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	29	31	PF00675	0.167
CLV_NRD_NRD_1	591	593	PF00675	0.624
CLV_PCSK_KEX2_1	551	553	PF00082	0.717
CLV_PCSK_KEX2_1	591	593	PF00082	0.666
CLV_PCSK_PC1ET2_1	551	553	PF00082	0.658
CLV_PCSK_SKI1_1	100	104	PF00082	0.249
CLV_PCSK_SKI1_1	132	136	PF00082	0.249
CLV_PCSK_SKI1_1	143	147	PF00082	0.236
CLV_PCSK_SKI1_1	182	186	PF00082	0.199
CLV_PCSK_SKI1_1	572	576	PF00082	0.548
CLV_Separin_Metazoa	349	353	PF03568	0.417
DEG_APCC_DBOX_1	346	354	PF00400	0.420
DEG_SCF_FBW7_2	74	81	PF00400	0.406
DOC_CDC14_PxL_1	319	327	PF14671	0.380
DOC_CKS1_1	22	27	PF01111	0.481
DOC_CKS1_1	75	80	PF01111	0.406
DOC_CYCLIN_RxL_1	129	136	PF00134	0.447
DOC_CYCLIN_RxL_1	144	158	PF00134	0.388
DOC_CYCLIN_yCln2_LP_2	175	178	PF00134	0.473
DOC_MAPK_DCC_7	397	406	PF00069	0.417
DOC_MAPK_gen_1	130	137	PF00069	0.497
DOC_MAPK_gen_1	180	187	PF00069	0.430
DOC_MAPK_gen_1	428	438	PF00069	0.517
DOC_MAPK_gen_1	452	460	PF00069	0.333
DOC_MAPK_HePTP_8	394	406	PF00069	0.417
DOC_MAPK_MEF2A_6	347	355	PF00069	0.406
DOC_MAPK_MEF2A_6	397	406	PF00069	0.417
DOC_PP2B_LxvP_1	175	178	PF13499	0.450
DOC_PP2B_LxvP_1	198	201	PF13499	0.567
DOC_PP2B_LxvP_1	314	317	PF13499	0.316
DOC_PP2B_LxvP_1	399	402	PF13499	0.406
DOC_PP4_FxxP_1	303	306	PF00568	0.536
DOC_PP4_FxxP_1	56	59	PF00568	0.406
DOC_PP4_FxxP_1	93	96	PF00568	0.473
DOC_USP7_MATH_1	208	212	PF00917	0.607
DOC_USP7_MATH_1	275	279	PF00917	0.674
DOC_USP7_MATH_1	284	288	PF00917	0.635
DOC_USP7_MATH_1	361	365	PF00917	0.406
DOC_USP7_MATH_1	402	406	PF00917	0.542
DOC_USP7_MATH_1	466	470	PF00917	0.430
DOC_USP7_MATH_1	47	51	PF00917	0.431
DOC_USP7_MATH_1	513	517	PF00917	0.530
DOC_USP7_MATH_1	534	538	PF00917	0.684
DOC_USP7_MATH_1	553	557	PF00917	0.730
DOC_USP7_MATH_1	579	583	PF00917	0.740
DOC_USP7_MATH_1	587	591	PF00917	0.626
DOC_USP7_MATH_1	605	609	PF00917	0.476
DOC_USP7_UBL2_3	572	576	PF12436	0.613
DOC_WW_Pin1_4	124	129	PF00397	0.406
DOC_WW_Pin1_4	21	26	PF00397	0.365
DOC_WW_Pin1_4	224	229	PF00397	0.626
DOC_WW_Pin1_4	460	465	PF00397	0.516
DOC_WW_Pin1_4	516	521	PF00397	0.667
DOC_WW_Pin1_4	530	535	PF00397	0.621
DOC_WW_Pin1_4	540	545	PF00397	0.717
DOC_WW_Pin1_4	608	613	PF00397	0.782
DOC_WW_Pin1_4	74	79	PF00397	0.406
LIG_14-3-3_CanoR_1	11	20	PF00244	0.340
LIG_14-3-3_CanoR_1	159	166	PF00244	0.437
LIG_14-3-3_CanoR_1	194	199	PF00244	0.506
LIG_14-3-3_CanoR_1	352	356	PF00244	0.431
LIG_14-3-3_CanoR_1	591	599	PF00244	0.591
LIG_APCC_ABBA_1	406	411	PF00400	0.417
LIG_BIR_II_1	1	5	PF00653	0.381
LIG_BIR_III_4	67	71	PF00653	0.453
LIG_BRCT_BRCA1_1	311	315	PF00533	0.235
LIG_eIF4E_1	69	75	PF01652	0.406
LIG_FHA_1	186	192	PF00498	0.430
LIG_FHA_1	256	262	PF00498	0.407
LIG_FHA_1	352	358	PF00498	0.438
LIG_FHA_1	455	461	PF00498	0.376
LIG_FHA_2	159	165	PF00498	0.406
LIG_FHA_2	194	200	PF00498	0.472
LIG_FHA_2	438	444	PF00498	0.350
LIG_FHA_2	557	563	PF00498	0.783
LIG_IRF3_LxIS_1	381	386	PF10401	0.452
LIG_LIR_Apic_2	301	306	PF02991	0.569
LIG_LIR_Apic_2	459	464	PF02991	0.423
LIG_LIR_Apic_2	53	59	PF02991	0.406
LIG_LIR_Gen_1	18	27	PF02991	0.428
LIG_LIR_Gen_1	243	253	PF02991	0.446
LIG_LIR_Gen_1	3	9	PF02991	0.381
LIG_LIR_Gen_1	310	320	PF02991	0.425
LIG_LIR_Gen_1	81	92	PF02991	0.406
LIG_LIR_Nem_3	18	22	PF02991	0.429
LIG_LIR_Nem_3	243	248	PF02991	0.468
LIG_LIR_Nem_3	3	7	PF02991	0.368
LIG_LIR_Nem_3	310	316	PF02991	0.429
LIG_LIR_Nem_3	67	72	PF02991	0.409
LIG_LIR_Nem_3	81	87	PF02991	0.415
LIG_LIR_Nem_3	88	93	PF02991	0.406
LIG_LYPXL_SIV_4	323	331	PF13949	0.453
LIG_NRBOX	130	136	PF00104	0.473
LIG_NRBOX	364	370	PF00104	0.510
LIG_RPA_C_Plants	496	507	PF08784	0.527
LIG_SH2_CRK	410	414	PF00017	0.429
LIG_SH2_GRB2like	341	344	PF00017	0.510
LIG_SH2_SRC	341	344	PF00017	0.510
LIG_SH2_SRC	84	87	PF00017	0.431
LIG_SH2_STAP1	442	446	PF00017	0.360
LIG_SH2_STAT5	151	154	PF00017	0.512
LIG_SH2_STAT5	313	316	PF00017	0.330
LIG_SH2_STAT5	324	327	PF00017	0.460
LIG_SH2_STAT5	341	344	PF00017	0.492
LIG_SH2_STAT5	419	422	PF00017	0.406
LIG_SH3_2	294	299	PF14604	0.560
LIG_SH3_3	134	140	PF00018	0.511
LIG_SH3_3	225	231	PF00018	0.473
LIG_SH3_3	291	297	PF00018	0.571
LIG_SH3_3	317	323	PF00018	0.448
LIG_SH3_3	72	78	PF00018	0.406
LIG_SUMO_SIM_anti_2	260	265	PF11976	0.490
LIG_SUMO_SIM_anti_2	434	441	PF11976	0.360
LIG_SUMO_SIM_anti_2	443	448	PF11976	0.234
LIG_TRAF2_1	267	270	PF00917	0.460
LIG_TYR_ITIM	149	154	PF00017	0.510
LIG_TYR_ITIM	82	87	PF00017	0.406
LIG_WRC_WIRS_1	378	383	PF05994	0.417
LIG_WW_2	228	231	PF00397	0.469
MOD_CDC14_SPxK_1	611	614	PF00782	0.630
MOD_CDK_SPK_2	516	521	PF00069	0.505
MOD_CDK_SPxK_1	124	130	PF00069	0.406
MOD_CDK_SPxK_1	608	614	PF00069	0.634
MOD_CDK_SPxK_1	74	80	PF00069	0.406
MOD_CDK_SPxxK_3	23	30	PF00069	0.215
MOD_CDK_SPxxK_3	460	467	PF00069	0.498
MOD_CK1_1	211	217	PF00069	0.781
MOD_CK1_1	221	227	PF00069	0.597
MOD_CK1_1	287	293	PF00069	0.667
MOD_CK1_1	364	370	PF00069	0.406
MOD_CK1_1	50	56	PF00069	0.406
MOD_CK1_1	516	522	PF00069	0.635
MOD_CK1_1	556	562	PF00069	0.642
MOD_CK1_1	590	596	PF00069	0.776
MOD_CK1_1	608	614	PF00069	0.717
MOD_CK2_1	193	199	PF00069	0.557
MOD_CK2_1	254	260	PF00069	0.527
MOD_GlcNHglycan	210	213	PF01048	0.632
MOD_GlcNHglycan	277	280	PF01048	0.679
MOD_GlcNHglycan	289	292	PF01048	0.690
MOD_GlcNHglycan	468	471	PF01048	0.432
MOD_GlcNHglycan	479	482	PF01048	0.364
MOD_GlcNHglycan	49	52	PF01048	0.245
MOD_GlcNHglycan	515	518	PF01048	0.488
MOD_GlcNHglycan	528	531	PF01048	0.757
MOD_GlcNHglycan	547	550	PF01048	0.692
MOD_GlcNHglycan	555	558	PF01048	0.740
MOD_GlcNHglycan	602	605	PF01048	0.701
MOD_GSK3_1	11	18	PF00069	0.496
MOD_GSK3_1	207	214	PF00069	0.770
MOD_GSK3_1	218	225	PF00069	0.792
MOD_GSK3_1	251	258	PF00069	0.468
MOD_GSK3_1	285	292	PF00069	0.682
MOD_GSK3_1	305	312	PF00069	0.353
MOD_GSK3_1	452	459	PF00069	0.326
MOD_GSK3_1	526	533	PF00069	0.611
MOD_GSK3_1	534	541	PF00069	0.646
MOD_GSK3_1	552	559	PF00069	0.540
MOD_GSK3_1	587	594	PF00069	0.587
MOD_LATS_1	192	198	PF00433	0.627
MOD_N-GLC_2	343	345	PF02516	0.206
MOD_NEK2_1	185	190	PF00069	0.424
MOD_NEK2_1	222	227	PF00069	0.558
MOD_NEK2_1	309	314	PF00069	0.266
MOD_NEK2_1	383	388	PF00069	0.453
MOD_NEK2_1	538	543	PF00069	0.761
MOD_PIKK_1	307	313	PF00454	0.354
MOD_PIKK_1	367	373	PF00454	0.417
MOD_PKA_1	452	458	PF00069	0.324
MOD_PKA_1	591	597	PF00069	0.508
MOD_PKA_2	158	164	PF00069	0.435
MOD_PKA_2	193	199	PF00069	0.555
MOD_PKA_2	298	304	PF00069	0.588
MOD_PKA_2	351	357	PF00069	0.431
MOD_PKA_2	553	559	PF00069	0.717
MOD_PKA_2	590	596	PF00069	0.673
MOD_PKA_2	597	603	PF00069	0.687
MOD_Plk_1	254	260	PF00069	0.428
MOD_Plk_1	442	448	PF00069	0.417
MOD_Plk_2-3	158	164	PF00069	0.455
MOD_Plk_2-3	255	261	PF00069	0.460
MOD_Plk_4	15	21	PF00069	0.556
MOD_Plk_4	168	174	PF00069	0.482
MOD_Plk_4	309	315	PF00069	0.278
MOD_Plk_4	335	341	PF00069	0.406
MOD_Plk_4	364	370	PF00069	0.419
MOD_Plk_4	402	408	PF00069	0.510
MOD_Plk_4	415	421	PF00069	0.361
MOD_Plk_4	442	448	PF00069	0.417
MOD_Plk_4	456	462	PF00069	0.402
MOD_ProDKin_1	124	130	PF00069	0.406
MOD_ProDKin_1	21	27	PF00069	0.364
MOD_ProDKin_1	224	230	PF00069	0.626
MOD_ProDKin_1	460	466	PF00069	0.514
MOD_ProDKin_1	516	522	PF00069	0.670
MOD_ProDKin_1	530	536	PF00069	0.623
MOD_ProDKin_1	540	546	PF00069	0.716
MOD_ProDKin_1	608	614	PF00069	0.784
MOD_ProDKin_1	74	80	PF00069	0.406
MOD_SUMO_rev_2	136	145	PF00179	0.335
MOD_SUMO_rev_2	445	455	PF00179	0.372
TRG_DiLeu_BaEn_1	243	248	PF01217	0.468
TRG_DiLeu_BaLyEn_6	171	176	PF01217	0.507
TRG_DiLeu_BaLyEn_6	470	475	PF01217	0.407
TRG_ENDOCYTIC_2	151	154	PF00928	0.515
TRG_ENDOCYTIC_2	19	22	PF00928	0.383
TRG_ENDOCYTIC_2	313	316	PF00928	0.336
TRG_ENDOCYTIC_2	69	72	PF00928	0.408
TRG_ENDOCYTIC_2	84	87	PF00928	0.401
TRG_ER_diArg_1	614	617	PF00400	0.635
TRG_ER_diLys_1	618	621	PF00400	0.507
TRG_NLS_MonoExtC_3	571	576	PF00514	0.615
TRG_Pf-PMV_PEXEL_1	11	15	PF00026	0.325
TRG_Pf-PMV_PEXEL_1	132	136	PF00026	0.268
TRG_Pf-PMV_PEXEL_1	150	155	PF00026	0.171
TRG_Pf-PMV_PEXEL_1	581	586	PF00026	0.578
TRG_Pf-PMV_PEXEL_1	80	85	PF00026	0.206

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2T9	Leptomonas seymouri	73%	100%
A0A0S4J5D6	Bodo saltans	49%	100%
A0A0S4KKU4	Bodo saltans	29%	89%
A0A1X0NVK0	Trypanosomatidae	28%	86%
A0A1X0P0F9	Trypanosomatidae	53%	100%
A0A3R7KKL1	Trypanosoma rangeli	32%	87%
A0A3R7M1K3	Trypanosoma rangeli	29%	87%
A0A3S5H5H4	Leishmania donovani	91%	99%
A0A3S7X5R1	Leishmania donovani	27%	80%
A0A422NDK5	Trypanosoma rangeli	55%	100%
A2XVF7	Oryza sativa subsp. indica	30%	75%
A3AVH5	Oryza sativa subsp. japonica	30%	75%
A3LNL1	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	29%	100%
A4H481	Leishmania braziliensis	80%	100%
A4HKL3	Leishmania braziliensis	28%	81%
A4HSF8	Leishmania infantum	91%	99%
A4I846	Leishmania infantum	27%	80%
A4R5B8	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	32%	68%
A6ZPU3	Saccharomyces cerevisiae (strain YJM789)	27%	81%
A7ESL7	Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1)	32%	68%
A7TJ71	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	28%	81%
C9ZNU6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	97%
D0A4H6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
D0AAB3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	86%
E9AQG1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	95%
E9B304	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	80%
P20448	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	81%
P26802	Drosophila melanogaster	29%	90%
Q0CNX1	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	38%	100%
Q0DLB9	Oryza sativa subsp. japonica	27%	100%
Q4HZ42	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	31%	69%
Q4IJH1	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	28%	100%
Q4Q552	Leishmania major	28%	80%
Q4QFH1	Leishmania major	30%	96%
Q4QJG6	Leishmania major	90%	100%
Q54BD6	Dictyostelium discoideum	25%	100%
Q6BL34	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	31%	67%
Q6C835	Yarrowia lipolytica (strain CLIB 122 / E 150)	29%	78%
Q6FPT7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	27%	81%
Q6FU81	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	26%	93%
Q6P9R1	Mus musculus	27%	97%
Q76PD3	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
Q7XJN0	Arabidopsis thaliana	26%	100%
Q8N8A6	Homo sapiens	26%	93%
Q93Y39	Arabidopsis thaliana	31%	75%
Q9H8H2	Homo sapiens	27%	73%
Q9Y7T7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	94%
V5CZW7	Trypanosoma cruzi	59%	100%
V5DCA1	Trypanosoma cruzi	28%	86%