Putative RNA-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Double RNA binding domain protein 3

Species:

Leishmania mexicana

UniProt:

E9AK98_LEIMU

TriTrypDb:

LmxM.04.1170

Length:

301

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AK98

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AK98

Function

Biological processes
Term	Name	Level	Count
GO:0009892	negative regulation of metabolic process	4	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0009894	regulation of catabolic process	4	1
GO:0009895	negative regulation of catabolic process	5	1
GO:0010468	regulation of gene expression	5	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0010605	negative regulation of macromolecule metabolic process	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0010628	positive regulation of gene expression	6	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031324	negative regulation of cellular metabolic process	5	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031330	negative regulation of cellular catabolic process	6	1
GO:0043487	regulation of RNA stability	3	1
GO:0043488	regulation of mRNA stability	4	1
GO:0043489	RNA stabilization	4	1
GO:0045934	negative regulation of nucleobase-containing compound metabolic process	6	1
GO:0048255	mRNA stabilization	5	1
GO:0048518	positive regulation of biological process	3	1
GO:0048519	negative regulation of biological process	3	1
GO:0048523	negative regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051172	negative regulation of nitrogen compound metabolic process	5	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0051253	negative regulation of RNA metabolic process	6	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0061013	regulation of mRNA catabolic process	6	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1902369	negative regulation of RNA catabolic process	7	1
GO:1902373	negative regulation of mRNA catabolic process	7	1
GO:1903311	regulation of mRNA metabolic process	6	1
GO:1903312	negative regulation of mRNA metabolic process	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003729	mRNA binding	5	1
GO:0003730	mRNA 3'-UTR binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	141	147	PF00089	0.548
CLV_NRD_NRD_1	139	141	PF00675	0.410
CLV_PCSK_KEX2_1	139	141	PF00082	0.410
CLV_PCSK_SKI1_1	159	163	PF00082	0.418
CLV_PCSK_SKI1_1	228	232	PF00082	0.338
CLV_PCSK_SKI1_1	78	82	PF00082	0.281
DEG_APCC_DBOX_1	158	166	PF00400	0.527
DEG_Nend_Nbox_1	1	3	PF02207	0.626
DEG_SCF_FBW7_1	80	85	PF00400	0.481
DOC_CYCLIN_RxL_1	156	164	PF00134	0.398
DOC_MAPK_gen_1	139	145	PF00069	0.547
DOC_PP2B_LxvP_1	15	18	PF13499	0.608
DOC_PP4_FxxP_1	128	131	PF00568	0.532
DOC_PP4_MxPP_1	294	297	PF00568	0.722
DOC_USP7_MATH_1	220	224	PF00917	0.528
DOC_USP7_MATH_1	261	265	PF00917	0.545
DOC_WW_Pin1_4	114	119	PF00397	0.528
DOC_WW_Pin1_4	13	18	PF00397	0.665
DOC_WW_Pin1_4	176	181	PF00397	0.334
DOC_WW_Pin1_4	78	83	PF00397	0.486
LIG_14-3-3_CanoR_1	144	151	PF00244	0.495
LIG_14-3-3_CanoR_1	274	280	PF00244	0.440
LIG_Clathr_ClatBox_1	215	219	PF01394	0.481
LIG_FHA_1	156	162	PF00498	0.429
LIG_FHA_1	177	183	PF00498	0.446
LIG_FHA_1	241	247	PF00498	0.418
LIG_FHA_1	260	266	PF00498	0.452
LIG_FHA_1	69	75	PF00498	0.520
LIG_FHA_1	79	85	PF00498	0.451
LIG_FHA_2	166	172	PF00498	0.460
LIG_FHA_2	235	241	PF00498	0.498
LIG_LIR_Apic_2	126	131	PF02991	0.534
LIG_LIR_Gen_1	130	141	PF02991	0.414
LIG_LIR_Gen_1	181	190	PF02991	0.548
LIG_LIR_Gen_1	247	256	PF02991	0.425
LIG_LIR_LC3C_4	262	267	PF02991	0.548
LIG_LIR_Nem_3	130	136	PF02991	0.406
LIG_LIR_Nem_3	181	187	PF02991	0.560
LIG_LIR_Nem_3	189	193	PF02991	0.540
LIG_LYPXL_S_1	294	298	PF13949	0.642
LIG_LYPXL_yS_3	295	298	PF13949	0.611
LIG_PTB_Apo_2	164	171	PF02174	0.435
LIG_PTB_Phospho_1	164	170	PF10480	0.428
LIG_REV1ctd_RIR_1	125	130	PF16727	0.433
LIG_SH2_CRK	2	6	PF00017	0.619
LIG_SH2_CRK	29	33	PF00017	0.719
LIG_SH2_GRB2like	33	36	PF00017	0.687
LIG_SH2_NCK_1	133	137	PF00017	0.409
LIG_SH2_NCK_1	29	33	PF00017	0.719
LIG_SH2_SRC	10	13	PF00017	0.674
LIG_SH2_SRC	133	136	PF00017	0.399
LIG_SH2_SRC	29	32	PF00017	0.662
LIG_SH2_SRC	33	36	PF00017	0.631
LIG_SH2_STAP1	170	174	PF00017	0.464
LIG_SH2_STAT5	142	145	PF00017	0.396
LIG_SH3_3	11	17	PF00018	0.642
LIG_SH3_3	194	200	PF00018	0.481
LIG_SUMO_SIM_anti_2	194	200	PF11976	0.548
LIG_SUMO_SIM_anti_2	20	27	PF11976	0.762
LIG_SUMO_SIM_par_1	158	164	PF11976	0.384
LIG_SUMO_SIM_par_1	234	241	PF11976	0.590
LIG_UBA3_1	112	119	PF00899	0.588
MOD_CDK_SPK_2	114	119	PF00069	0.528
MOD_CK1_1	117	123	PF00069	0.492
MOD_CK1_1	189	195	PF00069	0.543
MOD_CK2_1	234	240	PF00069	0.649
MOD_Cter_Amidation	137	140	PF01082	0.438
MOD_GlcNHglycan	119	122	PF01048	0.281
MOD_GlcNHglycan	171	175	PF01048	0.514
MOD_GlcNHglycan	222	225	PF01048	0.384
MOD_GSK3_1	13	20	PF00069	0.696
MOD_GSK3_1	151	158	PF00069	0.481
MOD_GSK3_1	161	168	PF00069	0.394
MOD_GSK3_1	234	241	PF00069	0.598
MOD_GSK3_1	255	262	PF00069	0.557
MOD_GSK3_1	283	290	PF00069	0.586
MOD_GSK3_1	78	85	PF00069	0.481
MOD_N-GLC_1	149	154	PF02516	0.538
MOD_N-GLC_1	201	206	PF02516	0.281
MOD_N-GLC_1	69	74	PF02516	0.537
MOD_N-GLC_1	78	83	PF02516	0.251
MOD_NEK2_1	151	156	PF00069	0.491
MOD_NEK2_1	161	166	PF00069	0.380
MOD_NEK2_1	238	243	PF00069	0.588
MOD_NEK2_1	289	294	PF00069	0.659
MOD_PIKK_1	151	157	PF00454	0.480
MOD_PIKK_1	165	171	PF00454	0.444
MOD_PIKK_1	202	208	PF00454	0.506
MOD_PIKK_1	56	62	PF00454	0.796
MOD_PIKK_1	82	88	PF00454	0.481
MOD_PKA_2	143	149	PF00069	0.505
MOD_PKA_2	220	226	PF00069	0.585
MOD_Plk_1	202	208	PF00069	0.585
MOD_Plk_1	69	75	PF00069	0.484
MOD_Plk_1	82	88	PF00069	0.467
MOD_Plk_4	161	167	PF00069	0.424
MOD_Plk_4	21	27	PF00069	0.747
MOD_Plk_4	261	267	PF00069	0.543
MOD_Plk_4	70	76	PF00069	0.673
MOD_ProDKin_1	114	120	PF00069	0.528
MOD_ProDKin_1	13	19	PF00069	0.667
MOD_ProDKin_1	176	182	PF00069	0.334
MOD_ProDKin_1	78	84	PF00069	0.486
MOD_SUMO_for_1	200	203	PF00179	0.492
MOD_SUMO_rev_2	251	259	PF00179	0.469
TRG_ENDOCYTIC_2	133	136	PF00928	0.390
TRG_ENDOCYTIC_2	142	145	PF00928	0.386
TRG_ENDOCYTIC_2	184	187	PF00928	0.516
TRG_ENDOCYTIC_2	190	193	PF00928	0.516
TRG_ENDOCYTIC_2	295	298	PF00928	0.611
TRG_ER_diArg_1	139	141	PF00400	0.410
TRG_ER_diArg_1	61	64	PF00400	0.656

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I013	Leptomonas seymouri	74%	93%
A0A0S4JUP2	Bodo saltans	68%	75%
A0A1X0NMJ6	Trypanosomatidae	67%	95%
A0A3R7NIY6	Trypanosoma rangeli	66%	93%
A0A3S7WNY0	Leishmania donovani	98%	97%
A4H432	Leishmania braziliensis	94%	100%
A4HSB6	Leishmania infantum	98%	97%
C9ZYA9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	92%
O97003	Leishmania major	98%	100%
V5BDL5	Trypanosoma cruzi	66%	96%