Adenylosuccinate lyase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

adenylosuccinate lyase, putative

Species:

Leishmania mexicana

UniProt:

E9AK27_LEIMU

TriTrypDb:

LmxM.04.0460

Length:

484

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AK27

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AK27

PDB structure(s): 4mx2_A , 4mx2_B , 4mx2_C , 4mx2_D , 4mx2_E , 4mx2_F , 4mx2_G , 4mx2_H

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006163	purine nucleotide metabolic process	5	11
GO:0006164	purine nucleotide biosynthetic process	6	11
GO:0006167	AMP biosynthetic process	8	11
GO:0006188	IMP biosynthetic process	8	11
GO:0006189	'de novo' IMP biosynthetic process	9	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006753	nucleoside phosphate metabolic process	4	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009117	nucleotide metabolic process	5	11
GO:0009123	nucleoside monophosphate metabolic process	5	11
GO:0009124	nucleoside monophosphate biosynthetic process	6	11
GO:0009126	purine nucleoside monophosphate metabolic process	6	11
GO:0009127	purine nucleoside monophosphate biosynthetic process	7	11
GO:0009150	purine ribonucleotide metabolic process	6	11
GO:0009152	purine ribonucleotide biosynthetic process	7	11
GO:0009156	ribonucleoside monophosphate biosynthetic process	7	11
GO:0009161	ribonucleoside monophosphate metabolic process	6	11
GO:0009165	nucleotide biosynthetic process	6	11
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	11
GO:0009168	purine ribonucleoside monophosphate biosynthetic process	8	11
GO:0009259	ribonucleotide metabolic process	5	11
GO:0009260	ribonucleotide biosynthetic process	6	11
GO:0009987	cellular process	1	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019637	organophosphate metabolic process	3	11
GO:0019693	ribose phosphate metabolic process	4	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0044208	'de novo' AMP biosynthetic process	9	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0044281	small molecule metabolic process	2	11
GO:0046033	AMP metabolic process	7	11
GO:0046040	IMP metabolic process	7	11
GO:0046390	ribose phosphate biosynthetic process	5	11
GO:0046483	heterocycle metabolic process	3	11
GO:0055086	nucleobase-containing small molecule metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0072521	purine-containing compound metabolic process	4	11
GO:0072522	purine-containing compound biosynthetic process	5	11
GO:0090407	organophosphate biosynthetic process	4	11
GO:1901135	carbohydrate derivative metabolic process	3	11
GO:1901137	carbohydrate derivative biosynthetic process	4	11
GO:1901293	nucleoside phosphate biosynthetic process	5	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004018	N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity	5	11
GO:0016829	lyase activity	2	11
GO:0016840	carbon-nitrogen lyase activity	3	11
GO:0016842	amidine-lyase activity	4	11
GO:0070626	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity	5	11
GO:0000166	nucleotide binding	3	1
GO:0005488	binding	1	1
GO:0036094	small molecule binding	2	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	367	371	PF00656	0.360
CLV_NRD_NRD_1	111	113	PF00675	0.288
CLV_NRD_NRD_1	271	273	PF00675	0.387
CLV_NRD_NRD_1	392	394	PF00675	0.424
CLV_NRD_NRD_1	425	427	PF00675	0.360
CLV_PCSK_FUR_1	45	49	PF00082	0.321
CLV_PCSK_KEX2_1	271	273	PF00082	0.307
CLV_PCSK_KEX2_1	392	394	PF00082	0.393
CLV_PCSK_KEX2_1	425	427	PF00082	0.360
CLV_PCSK_KEX2_1	47	49	PF00082	0.321
CLV_PCSK_PC1ET2_1	47	49	PF00082	0.321
CLV_PCSK_SKI1_1	112	116	PF00082	0.393
CLV_PCSK_SKI1_1	167	171	PF00082	0.387
CLV_PCSK_SKI1_1	225	229	PF00082	0.352
CLV_PCSK_SKI1_1	366	370	PF00082	0.380
CLV_PCSK_SKI1_1	397	401	PF00082	0.422
CLV_PCSK_SKI1_1	437	441	PF00082	0.357
CLV_PCSK_SKI1_1	88	92	PF00082	0.321
DEG_APCC_DBOX_1	224	232	PF00400	0.538
DEG_APCC_DBOX_1	465	473	PF00400	0.360
DEG_ODPH_VHL_1	141	152	PF01847	0.404
DOC_CYCLIN_yClb5_NLxxxL_5	375	381	PF00134	0.472
DOC_CYCLIN_yCln2_LP_2	377	383	PF00134	0.360
DOC_MAPK_gen_1	392	400	PF00069	0.424
DOC_MAPK_gen_1	64	71	PF00069	0.507
DOC_MAPK_MEF2A_6	64	71	PF00069	0.507
DOC_PP2B_LxvP_1	229	232	PF13499	0.559
DOC_PP2B_LxvP_1	377	380	PF13499	0.360
DOC_WW_Pin1_4	203	208	PF00397	0.507
DOC_WW_Pin1_4	37	42	PF00397	0.473
DOC_WW_Pin1_4	470	475	PF00397	0.360
LIG_14-3-3_CanoR_1	272	282	PF00244	0.508
LIG_14-3-3_CanoR_1	54	63	PF00244	0.523
LIG_14-3-3_CanoR_1	88	95	PF00244	0.508
LIG_14-3-3_CanoR_1	99	104	PF00244	0.506
LIG_Actin_WH2_2	378	394	PF00022	0.445
LIG_Actin_WH2_2	83	101	PF00022	0.504
LIG_BIR_II_1	1	5	PF00653	0.775
LIG_FHA_1	274	280	PF00498	0.507
LIG_FHA_1	460	466	PF00498	0.486
LIG_FHA_1	48	54	PF00498	0.504
LIG_FHA_2	119	125	PF00498	0.394
LIG_FHA_2	151	157	PF00498	0.499
LIG_FHA_2	275	281	PF00498	0.497
LIG_FHA_2	99	105	PF00498	0.592
LIG_GBD_Chelix_1	416	424	PF00786	0.360
LIG_IRF3_LxIS_1	355	362	PF10401	0.426
LIG_LIR_Apic_2	191	195	PF02991	0.523
LIG_LIR_Apic_2	427	432	PF02991	0.360
LIG_LIR_Gen_1	101	111	PF02991	0.588
LIG_LIR_Gen_1	144	152	PF02991	0.510
LIG_LIR_Gen_1	280	288	PF02991	0.507
LIG_LIR_Gen_1	305	315	PF02991	0.507
LIG_LIR_Gen_1	387	395	PF02991	0.216
LIG_LIR_Gen_1	410	420	PF02991	0.357
LIG_LIR_Gen_1	473	483	PF02991	0.360
LIG_LIR_Gen_1	58	67	PF02991	0.521
LIG_LIR_Nem_3	101	106	PF02991	0.588
LIG_LIR_Nem_3	144	150	PF02991	0.509
LIG_LIR_Nem_3	258	262	PF02991	0.534
LIG_LIR_Nem_3	265	270	PF02991	0.542
LIG_LIR_Nem_3	280	285	PF02991	0.507
LIG_LIR_Nem_3	305	310	PF02991	0.507
LIG_LIR_Nem_3	387	391	PF02991	0.216
LIG_LIR_Nem_3	410	416	PF02991	0.357
LIG_LIR_Nem_3	473	478	PF02991	0.360
LIG_LIR_Nem_3	58	63	PF02991	0.507
LIG_LYPXL_yS_3	251	254	PF13949	0.507
LIG_NRBOX	180	186	PF00104	0.580
LIG_Pex14_2	259	263	PF04695	0.507
LIG_PTAP_UEV_1	3	8	PF05743	0.475
LIG_PTB_Apo_2	31	38	PF02174	0.496
LIG_SH2_CRK	282	286	PF00017	0.538
LIG_SH2_CRK	480	484	PF00017	0.335
LIG_SH2_GRB2like	282	285	PF00017	0.538
LIG_SH2_PTP2	309	312	PF00017	0.610
LIG_SH2_STAP1	275	279	PF00017	0.517
LIG_SH2_STAP1	282	286	PF00017	0.495
LIG_SH2_STAT3	275	278	PF00017	0.538
LIG_SH2_STAT3	314	317	PF00017	0.587
LIG_SH2_STAT3	65	68	PF00017	0.587
LIG_SH2_STAT5	131	134	PF00017	0.510
LIG_SH2_STAT5	174	177	PF00017	0.496
LIG_SH2_STAT5	267	270	PF00017	0.507
LIG_SH2_STAT5	275	278	PF00017	0.538
LIG_SH2_STAT5	309	312	PF00017	0.507
LIG_SH2_STAT5	314	317	PF00017	0.507
LIG_SH2_STAT5	477	480	PF00017	0.433
LIG_SH2_STAT5	56	59	PF00017	0.521
LIG_SH2_STAT5	73	76	PF00017	0.507
LIG_SH3_3	1	7	PF00018	0.585
LIG_SH3_3	201	207	PF00018	0.507
LIG_SUMO_SIM_par_1	227	234	PF11976	0.440
LIG_SUMO_SIM_par_1	468	473	PF11976	0.380
LIG_TRAF2_1	6	9	PF00917	0.782
LIG_TRFH_1	37	41	PF08558	0.492
LIG_TYR_ITIM	478	483	PF00017	0.304
LIG_UBA3_1	181	188	PF00899	0.538
LIG_UBA3_1	227	236	PF00899	0.561
LIG_UBA3_1	298	304	PF00899	0.587
LIG_UBA3_1	351	358	PF00899	0.311
LIG_WRC_WIRS_1	385	390	PF05994	0.216
MOD_CK1_1	2	8	PF00069	0.765
MOD_CK1_1	230	236	PF00069	0.488
MOD_CK1_1	387	393	PF00069	0.216
MOD_CK2_1	2	8	PF00069	0.780
MOD_CK2_1	274	280	PF00069	0.507
MOD_CK2_1	98	104	PF00069	0.594
MOD_GlcNHglycan	136	139	PF01048	0.410
MOD_GlcNHglycan	326	329	PF01048	0.321
MOD_GlcNHglycan	355	358	PF01048	0.459
MOD_GlcNHglycan	4	7	PF01048	0.756
MOD_GSK3_1	13	20	PF00069	0.615
MOD_GSK3_1	227	234	PF00069	0.604
MOD_N-GLC_1	104	109	PF02516	0.401
MOD_N-GLC_1	157	162	PF02516	0.300
MOD_NEK2_1	150	155	PF00069	0.507
MOD_NEK2_1	184	189	PF00069	0.551
MOD_NEK2_1	197	202	PF00069	0.498
MOD_NEK2_1	227	232	PF00069	0.610
MOD_NEK2_1	262	267	PF00069	0.545
MOD_NEK2_1	345	350	PF00069	0.405
MOD_NEK2_1	368	373	PF00069	0.360
MOD_NEK2_1	98	103	PF00069	0.539
MOD_PIKK_1	151	157	PF00454	0.507
MOD_PIKK_1	274	280	PF00454	0.507
MOD_PIKK_1	82	88	PF00454	0.610
MOD_PKA_1	47	53	PF00069	0.521
MOD_PKA_2	273	279	PF00069	0.590
MOD_PKA_2	47	53	PF00069	0.504
MOD_PKA_2	98	104	PF00069	0.553
MOD_PKB_1	272	280	PF00069	0.610
MOD_Plk_1	104	110	PF00069	0.550
MOD_Plk_1	157	163	PF00069	0.500
MOD_Plk_1	369	375	PF00069	0.360
MOD_Plk_1	447	453	PF00069	0.384
MOD_Plk_2-3	104	110	PF00069	0.574
MOD_Plk_2-3	459	465	PF00069	0.504
MOD_Plk_4	294	300	PF00069	0.610
MOD_Plk_4	369	375	PF00069	0.360
MOD_Plk_4	419	425	PF00069	0.351
MOD_ProDKin_1	203	209	PF00069	0.507
MOD_ProDKin_1	37	43	PF00069	0.473
MOD_ProDKin_1	470	476	PF00069	0.360
MOD_SUMO_rev_2	118	128	PF00179	0.507
TRG_DiLeu_BaEn_3	222	228	PF01217	0.610
TRG_ENDOCYTIC_2	251	254	PF00928	0.507
TRG_ENDOCYTIC_2	267	270	PF00928	0.507
TRG_ENDOCYTIC_2	282	285	PF00928	0.507
TRG_ENDOCYTIC_2	309	312	PF00928	0.507
TRG_ENDOCYTIC_2	433	436	PF00928	0.360
TRG_ENDOCYTIC_2	480	483	PF00928	0.315
TRG_ENDOCYTIC_2	60	63	PF00928	0.507
TRG_ER_diArg_1	270	272	PF00400	0.587
TRG_ER_diArg_1	391	393	PF00400	0.424
TRG_ER_diArg_1	424	426	PF00400	0.360
TRG_Pf-PMV_PEXEL_1	112	116	PF00026	0.278
TRG_Pf-PMV_PEXEL_1	366	370	PF00026	0.360

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0H3AL67	Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)	48%	100%
A0A0N1I0W7	Leptomonas seymouri	83%	100%
A0A1X0NMR7	Trypanosomatidae	73%	100%
A0A3R7N1M2	Trypanosoma rangeli	73%	100%
A3KN12	Bos taurus	25%	99%
A4H3W5	Leishmania braziliensis	91%	100%
A4HS40	Leishmania infantum	94%	100%
A5IGB3	Legionella pneumophila (strain Corby)	47%	100%
A7LBL3	Leishmania donovani	94%	100%
C9ZY37	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	100%
O28041	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	30%	100%
O60105	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
P0AB89	Escherichia coli (strain K12)	49%	100%
P0AB90	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	49%	100%
P12047	Bacillus subtilis (strain 168)	26%	100%
P44797	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	48%	100%
P57351	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	43%	100%
P72478	Streptococcus mutans serotype c (strain ATCC 700610 / UA159)	29%	100%
Q2FFI7	Staphylococcus aureus (strain USA300)	25%	100%
Q2G2S0	Staphylococcus aureus (strain NCTC 8325 / PS 47)	25%	100%
Q2YU66	Staphylococcus aureus (strain bovine RF122 / ET3-1)	25%	100%
Q49YV3	Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)	25%	100%
Q4L7M3	Staphylococcus haemolyticus (strain JCSC1435)	25%	100%
Q54J34	Dictyostelium discoideum	56%	100%
Q58339	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	26%	100%
Q5HEL4	Staphylococcus aureus (strain COL)	25%	100%
Q5HN26	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	25%	100%
Q5ZXD1	Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)	47%	100%
Q6G825	Staphylococcus aureus (strain MSSA476)	25%	100%
Q6GFE9	Staphylococcus aureus (strain MRSA252)	25%	100%
Q7A0G9	Staphylococcus aureus (strain MW2)	25%	100%
Q7A4Q3	Staphylococcus aureus (strain N315)	25%	100%
Q89AM3	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	41%	100%
Q8CRT6	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	25%	100%
Q8K9Q7	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	43%	100%
Q99SX9	Staphylococcus aureus (strain Mu50 / ATCC 700699)	25%	100%
Q9I0K9	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	50%	100%
Q9RSE6	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	27%	100%
Q9U0T7	Leishmania major	94%	100%
Q9X0I0	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	27%	100%
V5BE01	Trypanosoma cruzi	73%	100%