Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania mexicana

UniProt:

E9AK26_LEIMU

TriTrypDb:

LmxM.04.0450

Length:

797

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 38

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 104
NetGPI	no	yes: 0, no: 106

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	5
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0005930	axoneme	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AK26

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AK26

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	107
GO:0006807	nitrogen compound metabolic process	2	107
GO:0008152	metabolic process	1	107
GO:0019538	protein metabolic process	3	107
GO:0043170	macromolecule metabolic process	3	107
GO:0044238	primary metabolic process	2	107
GO:0071704	organic substance metabolic process	2	107
GO:1901564	organonitrogen compound metabolic process	3	107

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	107
GO:0004175	endopeptidase activity	4	107
GO:0004197	cysteine-type endopeptidase activity	5	107
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	107
GO:0008233	peptidase activity	3	107
GO:0008234	cysteine-type peptidase activity	4	107
GO:0016787	hydrolase activity	2	107
GO:0140096	catalytic activity, acting on a protein	2	107

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	216	220	PF00656	0.504
CLV_C14_Caspase3-7	739	743	PF00656	0.581
CLV_NRD_NRD_1	27	29	PF00675	0.488
CLV_NRD_NRD_1	431	433	PF00675	0.455
CLV_PCSK_KEX2_1	255	257	PF00082	0.353
CLV_PCSK_PC1ET2_1	255	257	PF00082	0.373
CLV_PCSK_SKI1_1	211	215	PF00082	0.472
CLV_PCSK_SKI1_1	252	256	PF00082	0.303
CLV_PCSK_SKI1_1	29	33	PF00082	0.683
CLV_PCSK_SKI1_1	343	347	PF00082	0.381
DEG_Nend_UBRbox_1	1	4	PF02207	0.554
DOC_CDC14_PxL_1	677	685	PF14671	0.447
DOC_CYCLIN_yClb1_LxF_4	229	235	PF00134	0.480
DOC_CYCLIN_yCln2_LP_2	497	503	PF00134	0.596
DOC_MAPK_gen_1	33	43	PF00069	0.412
DOC_MAPK_gen_1	724	732	PF00069	0.490
DOC_MAPK_MEF2A_6	200	207	PF00069	0.443
DOC_MAPK_MEF2A_6	36	45	PF00069	0.671
DOC_MAPK_MEF2A_6	724	732	PF00069	0.516
DOC_PP1_RVXF_1	331	337	PF00149	0.526
DOC_PP1_RVXF_1	424	431	PF00149	0.580
DOC_PP1_RVXF_1	78	84	PF00149	0.686
DOC_PP2B_LxvP_1	43	46	PF13499	0.590
DOC_PP2B_LxvP_1	627	630	PF13499	0.315
DOC_PP2B_LxvP_1	678	681	PF13499	0.426
DOC_PP4_FxxP_1	24	27	PF00568	0.597
DOC_PP4_FxxP_1	314	317	PF00568	0.535
DOC_PP4_FxxP_1	558	561	PF00568	0.453
DOC_PP4_FxxP_1	98	101	PF00568	0.368
DOC_USP7_MATH_1	241	245	PF00917	0.495
DOC_USP7_MATH_1	512	516	PF00917	0.533
DOC_USP7_MATH_1	610	614	PF00917	0.551
DOC_USP7_UBL2_3	29	33	PF12436	0.632
DOC_USP7_UBL2_3	376	380	PF12436	0.529
DOC_USP7_UBL2_3	485	489	PF12436	0.596
DOC_WW_Pin1_4	136	141	PF00397	0.431
LIG_14-3-3_CanoR_1	28	35	PF00244	0.667
LIG_14-3-3_CanoR_1	333	337	PF00244	0.518
LIG_14-3-3_CanoR_1	567	573	PF00244	0.471
LIG_14-3-3_CanoR_1	718	724	PF00244	0.657
LIG_APCC_ABBA_1	769	774	PF00400	0.383
LIG_BRCT_BRCA1_1	120	124	PF00533	0.359
LIG_BRCT_BRCA1_1	250	254	PF00533	0.560
LIG_deltaCOP1_diTrp_1	653	658	PF00928	0.498
LIG_FHA_1	469	475	PF00498	0.540
LIG_FHA_1	535	541	PF00498	0.585
LIG_FHA_1	590	596	PF00498	0.545
LIG_FHA_1	665	671	PF00498	0.520
LIG_FHA_1	764	770	PF00498	0.515
LIG_FHA_1	89	95	PF00498	0.419
LIG_FHA_2	137	143	PF00498	0.334
LIG_FHA_2	145	151	PF00498	0.282
LIG_FHA_2	212	218	PF00498	0.529
LIG_FHA_2	300	306	PF00498	0.479
LIG_FHA_2	541	547	PF00498	0.620
LIG_FHA_2	569	575	PF00498	0.498
LIG_LIR_Apic_2	95	101	PF02991	0.336
LIG_LIR_Gen_1	375	386	PF02991	0.535
LIG_LIR_Gen_1	538	548	PF02991	0.541
LIG_LIR_Gen_1	635	646	PF02991	0.500
LIG_LIR_Gen_1	653	664	PF02991	0.489
LIG_LIR_Nem_3	236	242	PF02991	0.483
LIG_LIR_Nem_3	251	257	PF02991	0.595
LIG_LIR_Nem_3	348	354	PF02991	0.554
LIG_LIR_Nem_3	368	373	PF02991	0.524
LIG_LIR_Nem_3	37	41	PF02991	0.519
LIG_LIR_Nem_3	384	388	PF02991	0.529
LIG_LIR_Nem_3	538	544	PF02991	0.530
LIG_LIR_Nem_3	619	623	PF02991	0.498
LIG_LIR_Nem_3	635	641	PF02991	0.547
LIG_LIR_Nem_3	653	659	PF02991	0.512
LIG_LIR_Nem_3	759	764	PF02991	0.610
LIG_LYPXL_yS_3	620	623	PF13949	0.430
LIG_Pex14_2	169	173	PF04695	0.412
LIG_SH2_CRK	385	389	PF00017	0.525
LIG_SH2_CRK	435	439	PF00017	0.536
LIG_SH2_CRK	638	642	PF00017	0.576
LIG_SH2_GRB2like	64	67	PF00017	0.450
LIG_SH2_GRB2like	709	712	PF00017	0.340
LIG_SH2_NCK_1	385	389	PF00017	0.541
LIG_SH2_NCK_1	64	68	PF00017	0.716
LIG_SH2_PTP2	265	268	PF00017	0.570
LIG_SH2_SRC	64	67	PF00017	0.542
LIG_SH2_STAT5	155	158	PF00017	0.399
LIG_SH2_STAT5	190	193	PF00017	0.477
LIG_SH2_STAT5	265	268	PF00017	0.513
LIG_SH2_STAT5	350	353	PF00017	0.533
LIG_SH2_STAT5	550	553	PF00017	0.554
LIG_SH2_STAT5	677	680	PF00017	0.415
LIG_SH2_STAT5	709	712	PF00017	0.657
LIG_SH2_STAT5	777	780	PF00017	0.537
LIG_SH3_1	64	70	PF00018	0.489
LIG_SH3_3	105	111	PF00018	0.259
LIG_SH3_3	443	449	PF00018	0.563
LIG_SH3_3	582	588	PF00018	0.448
LIG_SH3_3	64	70	PF00018	0.706
LIG_SH3_3	759	765	PF00018	0.574
LIG_SH3_3	9	15	PF00018	0.702
LIG_SUMO_SIM_anti_2	742	748	PF11976	0.508
LIG_SUMO_SIM_par_1	480	486	PF11976	0.619
LIG_SUMO_SIM_par_1	621	626	PF11976	0.465
LIG_TRAF2_1	736	739	PF00917	0.330
LIG_TRAF2_1	771	774	PF00917	0.616
LIG_TRAF2_1	8	11	PF00917	0.570
LIG_TRFH_1	677	681	PF08558	0.430
LIG_TYR_ITIM	103	108	PF00017	0.386
LIG_WRC_WIRS_1	114	119	PF05994	0.404
LIG_WRC_WIRS_1	129	134	PF05994	0.462
LIG_WW_3	33	37	PF00397	0.640
MOD_CK1_1	136	142	PF00069	0.356
MOD_CK1_1	179	185	PF00069	0.389
MOD_CK1_1	245	251	PF00069	0.505
MOD_CK1_1	505	511	PF00069	0.564
MOD_CK2_1	136	142	PF00069	0.342
MOD_CK2_1	144	150	PF00069	0.347
MOD_CK2_1	211	217	PF00069	0.502
MOD_CK2_1	241	247	PF00069	0.550
MOD_CK2_1	299	305	PF00069	0.513
MOD_GlcNHglycan	181	184	PF01048	0.415
MOD_GlcNHglycan	247	250	PF01048	0.387
MOD_GlcNHglycan	321	324	PF01048	0.339
MOD_GlcNHglycan	389	392	PF01048	0.336
MOD_GlcNHglycan	418	421	PF01048	0.308
MOD_GlcNHglycan	441	444	PF01048	0.333
MOD_GlcNHglycan	504	507	PF01048	0.392
MOD_GlcNHglycan	644	647	PF01048	0.499
MOD_GlcNHglycan	671	675	PF01048	0.511
MOD_GlcNHglycan	71	74	PF01048	0.635
MOD_GSK3_1	211	218	PF00069	0.518
MOD_GSK3_1	241	248	PF00069	0.527
MOD_GSK3_1	434	441	PF00069	0.546
MOD_GSK3_1	589	596	PF00069	0.504
MOD_GSK3_1	642	649	PF00069	0.490
MOD_GSK3_1	777	784	PF00069	0.483
MOD_GSK3_1	88	95	PF00069	0.405
MOD_N-GLC_1	118	123	PF02516	0.372
MOD_N-GLC_1	632	637	PF02516	0.414
MOD_N-GLC_1	642	647	PF02516	0.490
MOD_N-GLC_1	718	723	PF02516	0.552
MOD_NEK2_1	141	146	PF00069	0.330
MOD_NEK2_1	157	162	PF00069	0.480
MOD_NEK2_1	341	346	PF00069	0.548
MOD_NEK2_1	381	386	PF00069	0.536
MOD_NEK2_1	439	444	PF00069	0.553
MOD_NEK2_1	468	473	PF00069	0.523
MOD_NEK2_1	581	586	PF00069	0.434
MOD_NEK2_1	593	598	PF00069	0.411
MOD_NEK2_1	703	708	PF00069	0.472
MOD_PIKK_1	184	190	PF00454	0.515
MOD_PIKK_1	2	8	PF00454	0.571
MOD_PIKK_1	299	305	PF00454	0.604
MOD_PIKK_1	646	652	PF00454	0.556
MOD_PKA_2	27	33	PF00069	0.625
MOD_PKA_2	332	338	PF00069	0.514
MOD_Plk_1	157	163	PF00069	0.406
MOD_Plk_1	235	241	PF00069	0.527
MOD_Plk_1	535	541	PF00069	0.535
MOD_Plk_1	589	595	PF00069	0.435
MOD_Plk_1	686	692	PF00069	0.519
MOD_Plk_1	718	724	PF00069	0.670
MOD_Plk_2-3	120	126	PF00069	0.323
MOD_Plk_2-3	451	457	PF00069	0.414
MOD_Plk_4	157	163	PF00069	0.394
MOD_Plk_4	434	440	PF00069	0.562
MOD_Plk_4	477	483	PF00069	0.550
MOD_Plk_4	535	541	PF00069	0.532
MOD_Plk_4	589	595	PF00069	0.424
MOD_Plk_4	703	709	PF00069	0.470
MOD_ProDKin_1	136	142	PF00069	0.431
MOD_SUMO_for_1	266	269	PF00179	0.596
MOD_SUMO_rev_2	305	310	PF00179	0.592
MOD_SUMO_rev_2	480	487	PF00179	0.576
TRG_DiLeu_BaEn_1	99	104	PF01217	0.380
TRG_DiLeu_BaEn_2	164	170	PF01217	0.308
TRG_DiLeu_BaEn_4	99	105	PF01217	0.380
TRG_ENDOCYTIC_2	105	108	PF00928	0.365
TRG_ENDOCYTIC_2	350	353	PF00928	0.550
TRG_ENDOCYTIC_2	385	388	PF00928	0.528
TRG_ENDOCYTIC_2	435	438	PF00928	0.527
TRG_ENDOCYTIC_2	479	482	PF00928	0.554
TRG_ENDOCYTIC_2	620	623	PF00928	0.457
TRG_ENDOCYTIC_2	638	641	PF00928	0.523
TRG_ENDOCYTIC_2	677	680	PF00928	0.406
TRG_ER_diArg_1	261	264	PF00400	0.560
TRG_ER_diArg_1	277	280	PF00400	0.535
TRG_Pf-PMV_PEXEL_1	364	368	PF00026	0.336

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	32%	100%
A0A0N1I8N2	Leptomonas seymouri	28%	81%
A0A0N1IGQ2	Leptomonas seymouri	30%	100%
A0A0N1ILF1	Leptomonas seymouri	35%	100%
A0A0N1IMH1	Leptomonas seymouri	31%	100%
A0A0N1P9P1	Leptomonas seymouri	72%	93%
A0A0N1PCA9	Leptomonas seymouri	33%	100%
A0A0N1PE91	Leptomonas seymouri	26%	90%
A0A0N1PFI4	Leptomonas seymouri	35%	100%
A0A0S4JLK6	Bodo saltans	27%	98%
A0A0S4JS70	Bodo saltans	25%	100%
A0A0S4KGT2	Bodo saltans	35%	100%
A0A1X0NJK2	Trypanosomatidae	34%	100%
A0A1X0NJX8	Trypanosomatidae	32%	100%
A0A1X0NKT7	Trypanosomatidae	30%	100%
A0A1X0NKX8	Trypanosomatidae	33%	97%
A0A1X0NMT3	Trypanosomatidae	49%	94%
A0A1X0NW84	Trypanosomatidae	36%	100%
A0A1X0NW85	Trypanosomatidae	35%	100%
A0A1X0NW89	Trypanosomatidae	44%	92%
A0A1X0NWA6	Trypanosomatidae	28%	91%
A0A1X0NWW1	Trypanosomatidae	38%	100%
A0A3Q8IBS3	Leishmania donovani	40%	88%
A0A3Q8IDD4	Leishmania donovani	31%	100%
A0A3Q8IJT4	Leishmania donovani	25%	100%
A0A3S5H5A5	Leishmania donovani	93%	93%
A0A3S5ISG2	Trypanosoma rangeli	32%	100%
A0A3S7WW13	Leishmania donovani	29%	72%
A0A3S7WW18	Leishmania donovani	40%	97%
A0A3S7WW41	Leishmania donovani	31%	100%
A0A3S7WW71	Leishmania donovani	33%	100%
A0A3S7X430	Leishmania donovani	33%	100%
A0A3S7X438	Leishmania donovani	32%	85%
A0A3S7X460	Leishmania donovani	30%	100%
A0A3S7X463	Leishmania donovani	29%	85%
A0A3S7X470	Leishmania donovani	34%	100%
A0A422MYU1	Trypanosoma rangeli	44%	87%
A0A422MYX0	Trypanosoma rangeli	35%	100%
A4H3W4	Leishmania braziliensis	83%	100%
A4HE81	Leishmania braziliensis	30%	100%
A4HFH7	Leishmania braziliensis	23%	100%
A4HJ14	Leishmania braziliensis	32%	100%
A4HJ21	Leishmania braziliensis	28%	100%
A4HJ22	Leishmania braziliensis	31%	100%
A4HJ24	Leishmania braziliensis	31%	100%
A4HS39	Leishmania infantum	93%	93%
A4HYN0	Leishmania infantum	40%	97%
A4HYW1	Leishmania infantum	40%	79%
A4HYW2	Leishmania infantum	33%	100%
A4HYW3	Leishmania infantum	31%	100%
A4HYW4	Leishmania infantum	29%	80%
A4I1J4	Leishmania infantum	30%	100%
A4I6E4	Leishmania infantum	34%	100%
A4I6E6	Leishmania infantum	30%	100%
A4I6F0	Leishmania infantum	32%	85%
A4I6K4	Leishmania infantum	33%	100%
A4I6K5	Leishmania infantum	29%	85%
A4I6K6	Leishmania infantum	25%	100%
C9ZIE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	74%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	93%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	99%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	93%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	98%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AIH1	Leishmania braziliensis	41%	100%
E9AIH3	Leishmania braziliensis	34%	95%
E9AIH4	Leishmania braziliensis	34%	100%
E9AIH6	Leishmania braziliensis	31%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	85%
E9AUQ8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	85%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
Q4Q6L7	Leishmania major	31%	100%
Q4Q6L9	Leishmania major	32%	100%
Q4Q6M0	Leishmania major	32%	100%
Q4Q6M2	Leishmania major	26%	100%
Q4Q6M3	Leishmania major	29%	100%
Q4Q6M4	Leishmania major	34%	100%
Q4Q9U3	Leishmania major	30%	96%
Q4QCS6	Leishmania major	31%	99%
Q4QCS7	Leishmania major	33%	95%
Q4QCS8	Leishmania major	40%	100%
Q4QCS9	Leishmania major	40%	100%
Q9U0T9	Leishmania major	93%	100%
V5AYJ1	Trypanosoma cruzi	30%	100%
V5B5I4	Trypanosoma cruzi	45%	95%
V5BA05	Trypanosoma cruzi	39%	100%
V5BEL3	Trypanosoma cruzi	35%	100%
V5BN20	Trypanosoma cruzi	32%	100%
V5D5V8	Trypanosoma cruzi	28%	100%
V5D9Y2	Trypanosoma cruzi	47%	94%
V5DES7	Trypanosoma cruzi	33%	100%