Glyco_hydro_32C domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein

Species:

Leishmania mexicana

UniProt:

E9AK14_LEIMU

TriTrypDb:

LmxM.04.0320

Length:

399

Annotations

LeishMANIAdb annotations

An expanded and variable family of glycosidases. Some Leishmaniid members even incorporate an extra N-terminal fructofuronidase domain after the signal peptide.. Seems to be evolving rapidly for unclear reasons

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AK14

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AK14

Function

Biological processes
Term	Name	Level	Count
GO:0005975	carbohydrate metabolic process	3	7
GO:0008152	metabolic process	1	8
GO:0044238	primary metabolic process	2	7
GO:0071704	organic substance metabolic process	2	7

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	4	7
GO:0016787	hydrolase activity	2	8
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	8
GO:0004564	beta-fructofuranosidase activity	5	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	75	77	PF00675	0.495
CLV_PCSK_KEX2_1	75	77	PF00082	0.493
CLV_PCSK_SKI1_1	113	117	PF00082	0.398
CLV_PCSK_SKI1_1	383	387	PF00082	0.572
CLV_PCSK_SKI1_1	52	56	PF00082	0.470
DEG_APCC_DBOX_1	279	287	PF00400	0.441
DEG_COP1_1	176	186	PF00400	0.391
DEG_Nend_Nbox_1	1	3	PF02207	0.279
DOC_MAPK_gen_1	124	134	PF00069	0.541
DOC_MAPK_HePTP_8	353	365	PF00069	0.387
DOC_MAPK_MEF2A_6	356	365	PF00069	0.387
DOC_PP1_RVXF_1	258	264	PF00149	0.441
DOC_PP4_FxxP_1	2	5	PF00568	0.477
DOC_PP4_FxxP_1	68	71	PF00568	0.495
DOC_USP7_MATH_1	213	217	PF00917	0.445
DOC_USP7_MATH_1	230	234	PF00917	0.368
DOC_USP7_MATH_1	235	239	PF00917	0.406
DOC_USP7_MATH_1	50	54	PF00917	0.491
DOC_USP7_UBL2_3	260	264	PF12436	0.441
DOC_WW_Pin1_4	139	144	PF00397	0.527
DOC_WW_Pin1_4	307	312	PF00397	0.478
DOC_WW_Pin1_4	375	380	PF00397	0.553
LIG_14-3-3_CanoR_1	162	166	PF00244	0.441
LIG_14-3-3_CanoR_1	52	57	PF00244	0.495
LIG_14-3-3_CanoR_1	75	79	PF00244	0.225
LIG_Actin_WH2_2	3	21	PF00022	0.447
LIG_APCC_ABBA_1	316	321	PF00400	0.340
LIG_APCC_ABBA_1	325	330	PF00400	0.340
LIG_BRCT_BRCA1_1	248	252	PF00533	0.481
LIG_BRCT_BRCA1_1	52	56	PF00533	0.425
LIG_FHA_1	115	121	PF00498	0.380
LIG_FHA_1	142	148	PF00498	0.558
LIG_FHA_1	215	221	PF00498	0.459
LIG_FHA_1	256	262	PF00498	0.398
LIG_FHA_1	295	301	PF00498	0.516
LIG_FHA_1	335	341	PF00498	0.365
LIG_FHA_1	346	352	PF00498	0.365
LIG_FHA_1	360	366	PF00498	0.365
LIG_FHA_1	384	390	PF00498	0.613
LIG_FHA_2	140	146	PF00498	0.559
LIG_FHA_2	162	168	PF00498	0.510
LIG_FHA_2	249	255	PF00498	0.420
LIG_FHA_2	276	282	PF00498	0.481
LIG_FHA_2	389	395	PF00498	0.405
LIG_FHA_2	53	59	PF00498	0.456
LIG_FHA_2	65	71	PF00498	0.337
LIG_FHA_2	7	13	PF00498	0.252
LIG_FHA_2	80	86	PF00498	0.320
LIG_LIR_Apic_2	371	377	PF02991	0.524
LIG_LIR_Apic_2	67	71	PF02991	0.468
LIG_LIR_Gen_1	15	24	PF02991	0.476
LIG_LIR_Gen_1	154	163	PF02991	0.441
LIG_LIR_Gen_1	197	207	PF02991	0.361
LIG_LIR_Gen_1	388	397	PF02991	0.613
LIG_LIR_Gen_1	41	50	PF02991	0.481
LIG_LIR_Gen_1	51	62	PF02991	0.347
LIG_LIR_Nem_3	15	20	PF02991	0.425
LIG_LIR_Nem_3	154	159	PF02991	0.502
LIG_LIR_Nem_3	197	202	PF02991	0.371
LIG_LIR_Nem_3	221	226	PF02991	0.373
LIG_LIR_Nem_3	28	32	PF02991	0.285
LIG_LIR_Nem_3	388	393	PF02991	0.610
LIG_LIR_Nem_3	41	47	PF02991	0.439
LIG_LIR_Nem_3	51	57	PF02991	0.413
LIG_LIR_Nem_3	58	62	PF02991	0.341
LIG_LIR_Nem_3	6	10	PF02991	0.392
LIG_LIR_Nem_3	67	72	PF02991	0.436
LIG_LYPXL_yS_3	223	226	PF13949	0.441
LIG_NRBOX	388	394	PF00104	0.564
LIG_PCNA_yPIPBox_3	100	114	PF02747	0.441
LIG_PDZ_Class_2	394	399	PF00595	0.670
LIG_PTB_Apo_2	384	391	PF02174	0.556
LIG_PTB_Phospho_1	384	390	PF10480	0.557
LIG_REV1ctd_RIR_1	5	15	PF16727	0.441
LIG_SH2_CRK	199	203	PF00017	0.391
LIG_SH2_CRK	69	73	PF00017	0.447
LIG_SH2_GRB2like	199	202	PF00017	0.225
LIG_SH2_NCK_1	199	203	PF00017	0.391
LIG_SH2_PTP2	44	47	PF00017	0.468
LIG_SH2_SRC	69	72	PF00017	0.447
LIG_SH2_STAP1	354	358	PF00017	0.429
LIG_SH2_STAT5	17	20	PF00017	0.425
LIG_SH2_STAT5	177	180	PF00017	0.384
LIG_SH2_STAT5	199	202	PF00017	0.332
LIG_SH2_STAT5	250	253	PF00017	0.405
LIG_SH2_STAT5	285	288	PF00017	0.441
LIG_SH2_STAT5	29	32	PF00017	0.351
LIG_SH2_STAT5	341	344	PF00017	0.340
LIG_SH2_STAT5	368	371	PF00017	0.564
LIG_SH2_STAT5	390	393	PF00017	0.400
LIG_SH2_STAT5	44	47	PF00017	0.447
LIG_SH3_1	264	270	PF00018	0.225
LIG_SH3_3	201	207	PF00018	0.365
LIG_SH3_3	264	270	PF00018	0.384
LIG_SH3_3	276	282	PF00018	0.340
LIG_SH3_3	44	50	PF00018	0.441
LIG_SUMO_SIM_par_1	361	366	PF11976	0.376
LIG_TYR_ITSM	152	159	PF00017	0.525
LIG_TYR_ITSM	65	72	PF00017	0.495
MOD_CK1_1	197	203	PF00069	0.444
MOD_CK1_1	388	394	PF00069	0.482
MOD_CK2_1	139	145	PF00069	0.494
MOD_CK2_1	248	254	PF00069	0.420
MOD_CK2_1	275	281	PF00069	0.441
MOD_CK2_1	320	326	PF00069	0.398
MOD_CK2_1	6	12	PF00069	0.252
MOD_CK2_1	79	85	PF00069	0.447
MOD_GlcNHglycan	171	174	PF01048	0.240
MOD_GlcNHglycan	211	214	PF01048	0.420
MOD_GlcNHglycan	232	235	PF01048	0.457
MOD_GlcNHglycan	237	240	PF01048	0.360
MOD_GlcNHglycan	275	278	PF01048	0.379
MOD_GlcNHglycan	290	293	PF01048	0.299
MOD_GlcNHglycan	96	99	PF01048	0.365
MOD_GSK3_1	137	144	PF00069	0.597
MOD_GSK3_1	169	176	PF00069	0.451
MOD_GSK3_1	18	25	PF00069	0.447
MOD_GSK3_1	209	216	PF00069	0.353
MOD_GSK3_1	290	297	PF00069	0.423
MOD_GSK3_1	388	395	PF00069	0.599
MOD_GSK3_1	48	55	PF00069	0.491
MOD_N-GLC_1	151	156	PF02516	0.547
MOD_N-GLC_1	200	205	PF02516	0.490
MOD_N-GLC_1	235	240	PF02516	0.395
MOD_N-GLC_1	241	246	PF02516	0.378
MOD_N-GLC_1	37	42	PF02516	0.397
MOD_N-GLC_1	383	388	PF02516	0.569
MOD_N-GLC_2	257	259	PF02516	0.441
MOD_NEK2_1	102	107	PF00069	0.441
MOD_NEK2_1	18	23	PF00069	0.447
MOD_NEK2_1	229	234	PF00069	0.382
MOD_NEK2_1	246	251	PF00069	0.172
MOD_NEK2_1	255	260	PF00069	0.298
MOD_NEK2_1	37	42	PF00069	0.447
MOD_NEK2_1	385	390	PF00069	0.513
MOD_NEK2_1	392	397	PF00069	0.559
MOD_NEK2_2	64	69	PF00069	0.495
MOD_PIKK_1	114	120	PF00454	0.441
MOD_PIKK_1	194	200	PF00454	0.495
MOD_PKA_2	161	167	PF00069	0.441
MOD_PKA_2	74	80	PF00069	0.333
MOD_PKB_1	288	296	PF00069	0.225
MOD_Plk_1	151	157	PF00069	0.523
MOD_Plk_1	194	200	PF00069	0.468
MOD_Plk_1	241	247	PF00069	0.369
MOD_Plk_1	320	326	PF00069	0.340
MOD_Plk_2-3	248	254	PF00069	0.225
MOD_Plk_2-3	366	372	PF00069	0.446
MOD_Plk_2-3	6	12	PF00069	0.252
MOD_Plk_4	151	157	PF00069	0.564
MOD_Plk_4	248	254	PF00069	0.441
MOD_Plk_4	320	326	PF00069	0.340
MOD_Plk_4	369	375	PF00069	0.446
MOD_Plk_4	385	391	PF00069	0.664
MOD_Plk_4	392	398	PF00069	0.645
MOD_Plk_4	64	70	PF00069	0.447
MOD_ProDKin_1	139	145	PF00069	0.530
MOD_ProDKin_1	307	313	PF00069	0.478
MOD_ProDKin_1	375	381	PF00069	0.558
MOD_SUMO_for_1	328	331	PF00179	0.441
MOD_SUMO_rev_2	109	115	PF00179	0.452
MOD_SUMO_rev_2	129	137	PF00179	0.268
TRG_DiLeu_BaEn_2	314	320	PF01217	0.441
TRG_DiLeu_BaLyEn_6	279	284	PF01217	0.468
TRG_ENDOCYTIC_2	156	159	PF00928	0.439
TRG_ENDOCYTIC_2	17	20	PF00928	0.425
TRG_ENDOCYTIC_2	199	202	PF00928	0.332
TRG_ENDOCYTIC_2	223	226	PF00928	0.384
TRG_ENDOCYTIC_2	390	393	PF00928	0.610
TRG_ENDOCYTIC_2	44	47	PF00928	0.498
TRG_ENDOCYTIC_2	59	62	PF00928	0.416
TRG_ENDOCYTIC_2	69	72	PF00928	0.398
TRG_NES_CRM1_1	129	141	PF08389	0.323
TRG_NES_CRM1_1	331	343	PF08389	0.441
TRG_Pf-PMV_PEXEL_1	124	129	PF00026	0.439

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I4P1	Leptomonas seymouri	49%	100%
A0A0S4J9J2	Bodo saltans	32%	88%
A0A1X0NKQ8	Trypanosomatidae	34%	100%
A0A1X0NYR4	Trypanosomatidae	35%	73%
A0A3S5H596	Leishmania donovani	90%	91%
A4HS27	Leishmania infantum	90%	82%
P49174	Zea mays	28%	68%
Q9XZX0	Leishmania major	90%	100%