DNA primase large subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA primase large subunit, putative

Species:

Leishmania mexicana

UniProt:

E9AJP4_LEIMU

TriTrypDb:

LmxM.03.0090

Length:

536

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0005658	alpha DNA polymerase:primase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0042575	DNA polymerase complex	3	12
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140513	nuclear protein-containing complex	2	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AJP4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AJP4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006269	DNA replication, synthesis of RNA primer	6	12
GO:0006270	DNA replication initiation	5	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0022402	cell cycle process	2	1
GO:0044786	cell cycle DNA replication	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0005488	binding	1	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003824	catalytic activity	1	2
GO:0003896	DNA primase activity	7	1
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	1
GO:0016740	transferase activity	2	2
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	2
GO:0016779	nucleotidyltransferase activity	4	2
GO:0034062	5'-3' RNA polymerase activity	5	1
GO:0097747	RNA polymerase activity	4	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	45	49	PF00656	0.482
CLV_C14_Caspase3-7	503	507	PF00656	0.461
CLV_NRD_NRD_1	190	192	PF00675	0.500
CLV_NRD_NRD_1	328	330	PF00675	0.328
CLV_NRD_NRD_1	483	485	PF00675	0.355
CLV_NRD_NRD_1	58	60	PF00675	0.566
CLV_NRD_NRD_1	87	89	PF00675	0.656
CLV_PCSK_KEX2_1	164	166	PF00082	0.431
CLV_PCSK_KEX2_1	190	192	PF00082	0.500
CLV_PCSK_KEX2_1	221	223	PF00082	0.401
CLV_PCSK_KEX2_1	23	25	PF00082	0.323
CLV_PCSK_KEX2_1	328	330	PF00082	0.328
CLV_PCSK_KEX2_1	483	485	PF00082	0.277
CLV_PCSK_KEX2_1	519	521	PF00082	0.581
CLV_PCSK_KEX2_1	58	60	PF00082	0.566
CLV_PCSK_KEX2_1	87	89	PF00082	0.656
CLV_PCSK_PC1ET2_1	164	166	PF00082	0.431
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.401
CLV_PCSK_PC1ET2_1	23	25	PF00082	0.310
CLV_PCSK_PC1ET2_1	519	521	PF00082	0.565
CLV_PCSK_PC7_1	217	223	PF00082	0.401
CLV_PCSK_SKI1_1	122	126	PF00082	0.314
CLV_PCSK_SKI1_1	140	144	PF00082	0.462
CLV_PCSK_SKI1_1	154	158	PF00082	0.519
CLV_PCSK_SKI1_1	190	194	PF00082	0.569
CLV_PCSK_SKI1_1	217	221	PF00082	0.285
CLV_PCSK_SKI1_1	222	226	PF00082	0.290
CLV_PCSK_SKI1_1	430	434	PF00082	0.262
DOC_CYCLIN_RxL_1	137	144	PF00134	0.431
DOC_MAPK_gen_1	519	525	PF00069	0.692
DOC_PP2B_LxvP_1	405	408	PF13499	0.297
DOC_PP4_FxxP_1	488	491	PF00568	0.262
DOC_USP7_MATH_1	197	201	PF00917	0.565
DOC_USP7_MATH_1	322	326	PF00917	0.204
DOC_USP7_MATH_1	511	515	PF00917	0.647
DOC_USP7_MATH_1	99	103	PF00917	0.584
DOC_USP7_MATH_2	491	497	PF00917	0.385
DOC_USP7_MATH_2	55	61	PF00917	0.653
DOC_WW_Pin1_4	34	39	PF00397	0.450
DOC_WW_Pin1_4	487	492	PF00397	0.262
LIG_14-3-3_CanoR_1	140	149	PF00244	0.426
LIG_14-3-3_CanoR_1	338	342	PF00244	0.198
LIG_14-3-3_CanoR_1	520	524	PF00244	0.649
LIG_14-3-3_CanoR_1	531	536	PF00244	0.598
LIG_BRCT_BRCA1_1	105	109	PF00533	0.523
LIG_BRCT_BRCA1_1	11	15	PF00533	0.510
LIG_BRCT_BRCA1_1	497	501	PF00533	0.440
LIG_Clathr_ClatBox_1	14	18	PF01394	0.381
LIG_CtBP_PxDLS_1	211	215	PF00389	0.401
LIG_deltaCOP1_diTrp_1	129	134	PF00928	0.350
LIG_FHA_1	114	120	PF00498	0.437
LIG_FHA_1	136	142	PF00498	0.449
LIG_FHA_1	155	161	PF00498	0.487
LIG_FHA_1	214	220	PF00498	0.284
LIG_FHA_1	338	344	PF00498	0.198
LIG_FHA_1	519	525	PF00498	0.568
LIG_FHA_2	11	17	PF00498	0.376
LIG_FHA_2	292	298	PF00498	0.395
LIG_FHA_2	449	455	PF00498	0.333
LIG_FHA_2	501	507	PF00498	0.590
LIG_LIR_Apic_2	205	211	PF02991	0.297
LIG_LIR_Apic_2	485	491	PF02991	0.262
LIG_LIR_Gen_1	240	250	PF02991	0.266
LIG_LIR_Gen_1	357	365	PF02991	0.297
LIG_LIR_Gen_1	42	51	PF02991	0.480
LIG_LIR_Gen_1	498	506	PF02991	0.396
LIG_LIR_Nem_3	185	189	PF02991	0.437
LIG_LIR_Nem_3	205	210	PF02991	0.120
LIG_LIR_Nem_3	240	245	PF02991	0.282
LIG_LIR_Nem_3	340	344	PF02991	0.214
LIG_LIR_Nem_3	357	362	PF02991	0.397
LIG_LIR_Nem_3	42	46	PF02991	0.406
LIG_LIR_Nem_3	490	495	PF02991	0.283
LIG_LIR_Nem_3	498	504	PF02991	0.384
LIG_LYPXL_yS_3	445	448	PF13949	0.355
LIG_PCNA_PIPBox_1	277	286	PF02747	0.262
LIG_PCNA_PIPBox_1	320	329	PF02747	0.198
LIG_Pex14_2	242	246	PF04695	0.277
LIG_Pex14_2	283	287	PF04695	0.262
LIG_RPA_C_Fungi	186	198	PF08784	0.540
LIG_SH2_CRK	189	193	PF00017	0.593
LIG_SH2_CRK	208	212	PF00017	0.179
LIG_SH2_NCK_1	396	400	PF00017	0.355
LIG_SH2_STAP1	495	499	PF00017	0.363
LIG_SH2_STAT5	186	189	PF00017	0.390
LIG_SH2_STAT5	223	226	PF00017	0.453
LIG_SH2_STAT5	258	261	PF00017	0.297
LIG_SH2_STAT5	396	399	PF00017	0.234
LIG_SH2_STAT5	467	470	PF00017	0.262
LIG_SH2_STAT5	474	477	PF00017	0.262
LIG_SH3_3	105	111	PF00018	0.547
LIG_SH3_3	166	172	PF00018	0.362
LIG_SUMO_SIM_par_1	210	216	PF11976	0.323
LIG_TRAF2_1	110	113	PF00917	0.469
LIG_TRAF2_1	266	269	PF00917	0.401
LIG_TRAF2_1	294	297	PF00917	0.297
LIG_TYR_ITIM	187	192	PF00017	0.508
LIG_UBA3_1	358	366	PF00899	0.276
MOD_CDK_SPxxK_3	34	41	PF00069	0.454
MOD_CK1_1	213	219	PF00069	0.401
MOD_CK1_1	395	401	PF00069	0.264
MOD_CK1_1	42	48	PF00069	0.451
MOD_CK1_1	505	511	PF00069	0.563
MOD_CK1_1	61	67	PF00069	0.643
MOD_CK1_1	91	97	PF00069	0.640
MOD_CK2_1	10	16	PF00069	0.420
MOD_CK2_1	193	199	PF00069	0.607
MOD_CK2_1	291	297	PF00069	0.342
MOD_CK2_1	487	493	PF00069	0.271
MOD_CMANNOS	131	134	PF00535	0.330
MOD_Cter_Amidation	390	393	PF01082	0.262
MOD_Cter_Amidation	517	520	PF01082	0.654
MOD_GlcNHglycan	195	198	PF01048	0.575
MOD_GlcNHglycan	288	291	PF01048	0.167
MOD_GlcNHglycan	397	400	PF01048	0.355
MOD_GlcNHglycan	451	454	PF01048	0.190
MOD_GlcNHglycan	509	512	PF01048	0.638
MOD_GlcNHglycan	513	516	PF01048	0.535
MOD_GlcNHglycan	60	63	PF01048	0.596
MOD_GlcNHglycan	77	82	PF01048	0.685
MOD_GlcNHglycan	96	99	PF01048	0.594
MOD_GSK3_1	193	200	PF00069	0.543
MOD_GSK3_1	268	275	PF00069	0.401
MOD_GSK3_1	344	351	PF00069	0.277
MOD_GSK3_1	444	451	PF00069	0.322
MOD_GSK3_1	483	490	PF00069	0.262
MOD_GSK3_1	507	514	PF00069	0.591
MOD_GSK3_1	57	64	PF00069	0.684
MOD_GSK3_1	82	89	PF00069	0.659
MOD_GSK3_1	99	106	PF00069	0.534
MOD_N-GLC_1	10	15	PF02516	0.481
MOD_NEK2_1	344	349	PF00069	0.298
MOD_NEK2_1	358	363	PF00069	0.224
MOD_NEK2_1	374	379	PF00069	0.262
MOD_NEK2_1	394	399	PF00069	0.120
MOD_NEK2_1	475	480	PF00069	0.240
MOD_NEK2_2	10	15	PF00069	0.437
MOD_PIKK_1	99	105	PF00454	0.591
MOD_PK_1	519	525	PF00069	0.640
MOD_PKA_1	392	398	PF00069	0.297
MOD_PKA_1	483	489	PF00069	0.277
MOD_PKA_1	519	525	PF00069	0.692
MOD_PKA_1	58	64	PF00069	0.608
MOD_PKA_1	87	93	PF00069	0.684
MOD_PKA_2	197	203	PF00069	0.556
MOD_PKA_2	337	343	PF00069	0.198
MOD_PKA_2	448	454	PF00069	0.158
MOD_PKA_2	483	489	PF00069	0.262
MOD_PKA_2	511	517	PF00069	0.676
MOD_PKA_2	519	525	PF00069	0.671
MOD_PKA_2	57	63	PF00069	0.687
MOD_PKA_2	86	92	PF00069	0.627
MOD_Plk_1	10	16	PF00069	0.519
MOD_Plk_1	352	358	PF00069	0.322
MOD_Plk_2-3	500	506	PF00069	0.299
MOD_Plk_4	10	16	PF00069	0.414
MOD_Plk_4	103	109	PF00069	0.545
MOD_Plk_4	241	247	PF00069	0.234
MOD_Plk_4	272	278	PF00069	0.262
MOD_Plk_4	358	364	PF00069	0.290
MOD_Plk_4	400	406	PF00069	0.262
MOD_Plk_4	42	48	PF00069	0.500
MOD_Plk_4	483	489	PF00069	0.262
MOD_Plk_4	519	525	PF00069	0.593
MOD_ProDKin_1	34	40	PF00069	0.448
MOD_ProDKin_1	487	493	PF00069	0.262
MOD_SUMO_for_1	147	150	PF00179	0.379
MOD_SUMO_for_1	192	195	PF00179	0.458
MOD_SUMO_rev_2	456	464	PF00179	0.358
TRG_DiLeu_BaLyEn_6	214	219	PF01217	0.277
TRG_ENDOCYTIC_2	189	192	PF00928	0.482
TRG_ENDOCYTIC_2	207	210	PF00928	0.152
TRG_ENDOCYTIC_2	341	344	PF00928	0.262
TRG_ENDOCYTIC_2	445	448	PF00928	0.369
TRG_ENDOCYTIC_2	472	475	PF00928	0.262
TRG_ENDOCYTIC_2	494	497	PF00928	0.347
TRG_ER_diArg_1	189	191	PF00400	0.474
TRG_ER_diArg_1	327	329	PF00400	0.328
TRG_ER_diArg_1	530	533	PF00400	0.549
TRG_ER_FFAT_2	281	293	PF00635	0.148
TRG_NLS_MonoExtN_4	217	224	PF00514	0.401

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDE0	Leptomonas seymouri	80%	93%
A0A0S4JD25	Bodo saltans	51%	94%
A0A1X0P8Q6	Trypanosomatidae	57%	91%
A0A3R7RF31	Trypanosoma rangeli	58%	94%
A0A3S7WNM4	Leishmania donovani	95%	95%
A4H3I1	Leishmania braziliensis	88%	100%
D0A216	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	93%
E9ACF6	Leishmania major	95%	100%
E9AG28	Leishmania infantum	95%	95%
O02334	Caenorhabditis elegans	28%	100%
O74761	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
O89044	Rattus norvegicus	33%	100%
P20457	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P33610	Mus musculus	33%	100%
P49643	Homo sapiens	31%	100%
Q55BM5	Dictyostelium discoideum	25%	100%
Q84WJ2	Arabidopsis thaliana	29%	100%
Q8NIZ4	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	27%	100%
Q9VPH2	Drosophila melanogaster	27%	100%
V5B8P2	Trypanosoma cruzi	58%	93%