tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Met-10+ like-protein, putative

Species:

Leishmania mexicana

UniProt:

E9AJP1_LEIMU

TriTrypDb:

LmxM.03.0060

Length:

321

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AJP1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AJP1

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006400	tRNA modification	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	3
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016740	transferase activity	2	12
GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3	12
GO:0102522	tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0008168	methyltransferase activity	4	3
GO:0008173	RNA methyltransferase activity	4	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0016741	transferase activity, transferring one-carbon groups	3	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	11	13	PF00675	0.403
CLV_NRD_NRD_1	65	67	PF00675	0.439
CLV_NRD_NRD_1	7	9	PF00675	0.449
CLV_NRD_NRD_1	87	89	PF00675	0.509
CLV_NRD_NRD_1	92	94	PF00675	0.461
CLV_NRD_NRD_1	97	99	PF00675	0.358
CLV_PCSK_KEX2_1	254	256	PF00082	0.284
CLV_PCSK_KEX2_1	310	312	PF00082	0.423
CLV_PCSK_KEX2_1	65	67	PF00082	0.473
CLV_PCSK_KEX2_1	7	9	PF00082	0.423
CLV_PCSK_KEX2_1	87	89	PF00082	0.494
CLV_PCSK_KEX2_1	92	94	PF00082	0.399
CLV_PCSK_KEX2_1	99	101	PF00082	0.255
CLV_PCSK_PC1ET2_1	254	256	PF00082	0.329
CLV_PCSK_PC1ET2_1	310	312	PF00082	0.423
CLV_PCSK_PC1ET2_1	99	101	PF00082	0.529
CLV_PCSK_PC7_1	88	94	PF00082	0.509
CLV_PCSK_SKI1_1	140	144	PF00082	0.254
CLV_PCSK_SKI1_1	243	247	PF00082	0.329
CLV_PCSK_SKI1_1	302	306	PF00082	0.351
CLV_PCSK_SKI1_1	7	11	PF00082	0.540
CLV_Separin_Metazoa	32	36	PF03568	0.234
DEG_APCC_DBOX_1	65	73	PF00400	0.474
DOC_MAPK_gen_1	65	73	PF00069	0.501
DOC_MAPK_MEF2A_6	65	73	PF00069	0.501
DOC_PP1_RVXF_1	138	144	PF00149	0.438
DOC_PP4_FxxP_1	174	177	PF00568	0.443
DOC_USP7_MATH_1	110	114	PF00917	0.524
DOC_USP7_MATH_1	258	262	PF00917	0.493
DOC_USP7_UBL2_3	95	99	PF12436	0.426
DOC_WW_Pin1_4	118	123	PF00397	0.426
DOC_WW_Pin1_4	43	48	PF00397	0.296
LIG_14-3-3_CanoR_1	117	124	PF00244	0.482
LIG_14-3-3_CanoR_1	248	253	PF00244	0.443
LIG_14-3-3_CanoR_1	297	304	PF00244	0.379
LIG_14-3-3_CanoR_1	87	91	PF00244	0.470
LIG_14-3-3_CanoR_1	98	103	PF00244	0.285
LIG_Actin_WH2_2	193	209	PF00022	0.493
LIG_Actin_WH2_2	267	284	PF00022	0.509
LIG_Actin_WH2_2	289	304	PF00022	0.457
LIG_BIR_II_1	1	5	PF00653	0.285
LIG_BRCT_BRCA1_1	120	124	PF00533	0.408
LIG_BRCT_BRCA1_1	195	199	PF00533	0.468
LIG_BRCT_BRCA1_2	195	201	PF00533	0.468
LIG_Clathr_ClatBox_1	54	58	PF01394	0.466
LIG_DLG_GKlike_1	65	72	PF00625	0.502
LIG_EH1_1	312	320	PF00400	0.378
LIG_FHA_1	123	129	PF00498	0.451
LIG_FHA_1	173	179	PF00498	0.438
LIG_FHA_2	156	162	PF00498	0.548
LIG_FHA_2	27	33	PF00498	0.476
LIG_LIR_Gen_1	196	206	PF02991	0.484
LIG_LIR_Gen_1	290	298	PF02991	0.228
LIG_LIR_Nem_3	166	171	PF02991	0.435
LIG_LIR_Nem_3	196	202	PF02991	0.443
LIG_LIR_Nem_3	290	295	PF02991	0.341
LIG_LIR_Nem_3	43	48	PF02991	0.427
LIG_Pex14_2	10	14	PF04695	0.392
LIG_Pex14_2	41	45	PF04695	0.376
LIG_SH2_CRK	267	271	PF00017	0.324
LIG_SH2_STAP1	134	138	PF00017	0.472
LIG_SH2_STAP1	82	86	PF00017	0.494
LIG_SH2_STAT5	173	176	PF00017	0.443
LIG_SH2_STAT5	238	241	PF00017	0.439
LIG_SUMO_SIM_anti_2	51	56	PF11976	0.290
LIG_SUMO_SIM_par_1	46	51	PF11976	0.407
LIG_SUMO_SIM_par_1	53	58	PF11976	0.338
LIG_TRAF2_1	276	279	PF00917	0.438
LIG_TYR_ITIM	171	176	PF00017	0.296
LIG_TYR_ITIM	265	270	PF00017	0.325
LIG_WRC_WIRS_1	133	138	PF05994	0.416
MOD_CK1_1	101	107	PF00069	0.472
MOD_CK1_1	145	151	PF00069	0.365
MOD_CK2_1	26	32	PF00069	0.505
MOD_GlcNHglycan	1	4	PF01048	0.293
MOD_GlcNHglycan	144	147	PF01048	0.296
MOD_GlcNHglycan	261	264	PF01048	0.429
MOD_GSK3_1	116	123	PF00069	0.347
MOD_GSK3_1	132	139	PF00069	0.276
MOD_GSK3_1	141	148	PF00069	0.112
MOD_GSK3_1	77	84	PF00069	0.481
MOD_N-GLC_1	146	151	PF02516	0.296
MOD_NEK2_1	111	116	PF00069	0.442
MOD_NEK2_1	136	141	PF00069	0.363
MOD_NEK2_1	142	147	PF00069	0.172
MOD_NEK2_1	41	46	PF00069	0.367
MOD_NEK2_1	86	91	PF00069	0.452
MOD_NEK2_2	82	87	PF00069	0.428
MOD_PIKK_1	98	104	PF00454	0.273
MOD_PKA_1	65	71	PF00069	0.479
MOD_PKA_1	98	104	PF00069	0.425
MOD_PKA_2	116	122	PF00069	0.518
MOD_PKA_2	259	265	PF00069	0.431
MOD_PKA_2	296	302	PF00069	0.488
MOD_PKA_2	65	71	PF00069	0.475
MOD_PKA_2	86	92	PF00069	0.462
MOD_Plk_1	111	117	PF00069	0.521
MOD_Plk_1	136	142	PF00069	0.399
MOD_Plk_2-3	27	33	PF00069	0.508
MOD_Plk_4	241	247	PF00069	0.339
MOD_Plk_4	248	254	PF00069	0.276
MOD_ProDKin_1	118	124	PF00069	0.420
MOD_ProDKin_1	43	49	PF00069	0.298
TRG_DiLeu_BaEn_3	290	296	PF01217	0.446
TRG_ENDOCYTIC_2	173	176	PF00928	0.296
TRG_ENDOCYTIC_2	267	270	PF00928	0.320
TRG_ER_diArg_1	64	66	PF00400	0.480
TRG_ER_diArg_1	7	9	PF00400	0.422
TRG_ER_diArg_1	86	88	PF00400	0.507
TRG_ER_diArg_1	92	94	PF00400	0.517
TRG_NLS_Bipartite_1	87	102	PF00514	0.467
TRG_NLS_MonoExtN_4	95	102	PF00514	0.267

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4L5	Leptomonas seymouri	70%	80%
A0A0S4JHI6	Bodo saltans	49%	78%
A0A1X0P9M7	Trypanosomatidae	56%	78%
A0A422N7F4	Trypanosoma rangeli	55%	82%
A0A451EJL2	Leishmania donovani	93%	82%
A4H3H8	Leishmania braziliensis	88%	100%
D0A212	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	81%
E9ACF3	Leishmania major	94%	100%
E9AG25	Leishmania infantum	93%	82%
Q0P466	Danio rerio	33%	79%
V5B8P6	Trypanosoma cruzi	55%	82%