Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FtsJ-like methyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9AJK1_LEIMU

TriTrypDb:

LmxM.02.0380

Length:

454

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

E9AJK1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AJK1

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	11
GO:0002128	tRNA nucleoside ribose methylation	6	11
GO:0002181	cytoplasmic translation	5	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006399	tRNA metabolic process	7	11
GO:0006400	tRNA modification	6	11
GO:0006412	translation	4	11
GO:0006518	peptide metabolic process	4	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008033	tRNA processing	8	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0019538	protein metabolic process	3	11
GO:0030488	tRNA methylation	5	11
GO:0032259	methylation	2	11
GO:0034470	ncRNA processing	7	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	11
GO:0034660	ncRNA metabolic process	6	11
GO:0043043	peptide biosynthetic process	5	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0043414	macromolecule methylation	3	11
GO:0043603	amide metabolic process	3	11
GO:0043604	amide biosynthetic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0008168	methyltransferase activity	4	11
GO:0008173	RNA methyltransferase activity	4	11
GO:0008175	tRNA methyltransferase activity	5	11
GO:0016740	transferase activity	2	11
GO:0016741	transferase activity, transferring one-carbon groups	3	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	336	340	PF00656	0.458
CLV_C14_Caspase3-7	423	427	PF00656	0.751
CLV_NRD_NRD_1	14	16	PF00675	0.389
CLV_NRD_NRD_1	159	161	PF00675	0.276
CLV_PCSK_KEX2_1	14	16	PF00082	0.389
CLV_PCSK_KEX2_1	431	433	PF00082	0.509
CLV_PCSK_KEX2_1	8	10	PF00082	0.453
CLV_PCSK_PC1ET2_1	431	433	PF00082	0.509
CLV_PCSK_PC1ET2_1	8	10	PF00082	0.453
CLV_PCSK_SKI1_1	14	18	PF00082	0.387
CLV_PCSK_SKI1_1	230	234	PF00082	0.292
DEG_APCC_DBOX_1	251	259	PF00400	0.447
DEG_SPOP_SBC_1	126	130	PF00917	0.396
DEG_SPOP_SBC_1	278	282	PF00917	0.543
DOC_CYCLIN_yCln2_LP_2	399	405	PF00134	0.577
DOC_MAPK_gen_1	160	166	PF00069	0.460
DOC_PP2B_LxvP_1	124	127	PF13499	0.526
DOC_PP2B_LxvP_1	399	402	PF13499	0.545
DOC_PP4_FxxP_1	210	213	PF00568	0.446
DOC_PP4_FxxP_1	251	254	PF00568	0.383
DOC_USP7_MATH_1	141	145	PF00917	0.537
DOC_USP7_MATH_1	234	238	PF00917	0.443
DOC_USP7_MATH_1	265	269	PF00917	0.632
DOC_USP7_MATH_1	279	283	PF00917	0.515
DOC_USP7_MATH_1	305	309	PF00917	0.733
DOC_USP7_MATH_1	409	413	PF00917	0.644
DOC_USP7_MATH_1	425	429	PF00917	0.678
DOC_WW_Pin1_4	149	154	PF00397	0.548
DOC_WW_Pin1_4	315	320	PF00397	0.567
DOC_WW_Pin1_4	37	42	PF00397	0.413
DOC_WW_Pin1_4	434	439	PF00397	0.683
DOC_WW_Pin1_4	84	89	PF00397	0.505
LIG_14-3-3_CanoR_1	211	219	PF00244	0.526
LIG_14-3-3_CanoR_1	22	30	PF00244	0.439
LIG_14-3-3_CanoR_1	238	243	PF00244	0.446
LIG_Actin_WH2_2	185	202	PF00022	0.460
LIG_Actin_WH2_2	300	315	PF00022	0.456
LIG_BRCT_BRCA1_1	142	146	PF00533	0.410
LIG_BRCT_BRCA1_1	221	225	PF00533	0.460
LIG_eIF4E_1	182	188	PF01652	0.446
LIG_FHA_1	110	116	PF00498	0.549
LIG_FHA_1	193	199	PF00498	0.446
LIG_FHA_1	213	219	PF00498	0.440
LIG_FHA_1	44	50	PF00498	0.448
LIG_FHA_1	85	91	PF00498	0.532
LIG_FHA_2	114	120	PF00498	0.434
LIG_FHA_2	131	137	PF00498	0.528
LIG_FHA_2	295	301	PF00498	0.542
LIG_FHA_2	343	349	PF00498	0.703
LIG_FHA_2	357	363	PF00498	0.685
LIG_FHA_2	421	427	PF00498	0.722
LIG_FHA_2	446	452	PF00498	0.701
LIG_LIR_Apic_2	207	213	PF02991	0.446
LIG_LIR_Gen_1	223	234	PF02991	0.480
LIG_LIR_Gen_1	34	44	PF02991	0.461
LIG_LIR_Gen_1	57	66	PF02991	0.446
LIG_LIR_Nem_3	223	229	PF02991	0.379
LIG_LIR_Nem_3	25	30	PF02991	0.446
LIG_LIR_Nem_3	34	39	PF02991	0.447
LIG_LIR_Nem_3	57	61	PF02991	0.446
LIG_LYPXL_SIV_4	402	410	PF13949	0.333
LIG_MLH1_MIPbox_1	142	146	PF16413	0.341
LIG_PCNA_PIPBox_1	176	185	PF02747	0.460
LIG_PCNA_yPIPBox_3	233	246	PF02747	0.446
LIG_SH2_CRK	27	31	PF00017	0.446
LIG_SH2_STAP1	326	330	PF00017	0.670
LIG_SH2_STAT5	145	148	PF00017	0.341
LIG_SH2_STAT5	182	185	PF00017	0.446
LIG_SH2_STAT5	257	260	PF00017	0.507
LIG_SH2_STAT5	324	327	PF00017	0.666
LIG_SH2_STAT5	403	406	PF00017	0.498
LIG_SH3_3	228	234	PF00018	0.513
LIG_SH3_3	316	322	PF00018	0.566
LIG_SH3_3	361	367	PF00018	0.486
LIG_SH3_3	388	394	PF00018	0.519
LIG_SH3_3	432	438	PF00018	0.691
LIG_SUMO_SIM_anti_2	102	108	PF11976	0.549
LIG_SUMO_SIM_par_1	89	94	PF11976	0.446
LIG_TRAF2_1	16	19	PF00917	0.382
LIG_TRAF2_1	297	300	PF00917	0.483
LIG_WRC_WIRS_1	194	199	PF05994	0.446
LIG_WRC_WIRS_1	242	247	PF05994	0.526
MOD_CDK_SPK_2	84	89	PF00069	0.549
MOD_CK1_1	113	119	PF00069	0.460
MOD_CK1_1	144	150	PF00069	0.509
MOD_CK1_1	241	247	PF00069	0.524
MOD_CK1_1	269	275	PF00069	0.687
MOD_CK1_1	315	321	PF00069	0.632
MOD_CK1_1	333	339	PF00069	0.707
MOD_CK2_1	113	119	PF00069	0.434
MOD_CK2_1	132	138	PF00069	0.534
MOD_CK2_1	284	290	PF00069	0.740
MOD_CK2_1	294	300	PF00069	0.503
MOD_CK2_1	37	43	PF00069	0.413
MOD_CK2_1	445	451	PF00069	0.704
MOD_DYRK1A_RPxSP_1	434	438	PF00069	0.683
MOD_GlcNHglycan	148	151	PF01048	0.288
MOD_GlcNHglycan	24	27	PF01048	0.237
MOD_GlcNHglycan	268	271	PF01048	0.668
MOD_GlcNHglycan	281	284	PF01048	0.484
MOD_GlcNHglycan	307	310	PF01048	0.698
MOD_GlcNHglycan	314	317	PF01048	0.630
MOD_GlcNHglycan	373	377	PF01048	0.495
MOD_GSK3_1	109	116	PF00069	0.445
MOD_GSK3_1	126	133	PF00069	0.416
MOD_GSK3_1	140	147	PF00069	0.536
MOD_GSK3_1	234	241	PF00069	0.446
MOD_GSK3_1	265	272	PF00069	0.558
MOD_GSK3_1	299	306	PF00069	0.727
MOD_GSK3_1	326	333	PF00069	0.678
MOD_GSK3_1	434	441	PF00069	0.588
MOD_N-GLC_1	238	243	PF02516	0.246
MOD_N-GLC_1	354	359	PF02516	0.429
MOD_NEK2_1	146	151	PF00069	0.488
MOD_NEK2_1	155	160	PF00069	0.396
MOD_NEK2_1	192	197	PF00069	0.446
MOD_NEK2_1	312	317	PF00069	0.711
MOD_PIKK_1	425	431	PF00454	0.722
MOD_PIKK_1	56	62	PF00454	0.446
MOD_PKA_2	2	8	PF00069	0.516
MOD_PKA_2	312	318	PF00069	0.674
MOD_PKA_2	420	426	PF00069	0.735
MOD_PKA_2	443	449	PF00069	0.703
MOD_Plk_1	238	244	PF00069	0.446
MOD_Plk_4	102	108	PF00069	0.494
MOD_Plk_4	141	147	PF00069	0.427
MOD_Plk_4	193	199	PF00069	0.446
MOD_Plk_4	241	247	PF00069	0.519
MOD_Plk_4	79	85	PF00069	0.341
MOD_ProDKin_1	149	155	PF00069	0.548
MOD_ProDKin_1	315	321	PF00069	0.570
MOD_ProDKin_1	37	43	PF00069	0.413
MOD_ProDKin_1	434	440	PF00069	0.684
MOD_ProDKin_1	84	90	PF00069	0.505
MOD_SUMO_for_1	16	19	PF00179	0.382
MOD_SUMO_for_1	220	223	PF00179	0.384
TRG_DiLeu_BaEn_1	119	124	PF01217	0.460
TRG_DiLeu_BaEn_1	79	84	PF01217	0.341
TRG_DiLeu_BaEn_4	34	40	PF01217	0.460
TRG_DiLeu_BaEn_4	387	393	PF01217	0.450
TRG_DiLeu_LyEn_5	119	124	PF01217	0.460
TRG_ENDOCYTIC_2	27	30	PF00928	0.434
TRG_ER_diArg_1	13	15	PF00400	0.414
TRG_ER_diArg_1	21	24	PF00400	0.443
TRG_ER_diArg_1	432	435	PF00400	0.505
TRG_NES_CRM1_1	96	110	PF08389	0.446
TRG_NLS_MonoExtC_3	430	436	PF00514	0.667

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTF7	Leptomonas seymouri	62%	100%
A0A0S4JD71	Bodo saltans	45%	100%
A0A1X0NJR5	Trypanosomatidae	46%	100%
A0A3S7X469	Leishmania donovani	24%	100%
A0A451EJH4	Leishmania donovani	90%	99%
A4H3D4	Leishmania braziliensis	76%	100%
A4HRN5	Leishmania infantum	90%	99%
A4I6F9	Leishmania infantum	24%	100%
C9ZJ17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9ACB3	Leishmania major	90%	100%
E9B1L6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
P38238	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	36%	100%
Q9BHC8	Leishmania major	24%	100%
V5BCX7	Trypanosoma cruzi	50%	100%