Putative cytochrome b-domain protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytochrome b-domain protein, putative

Species:

Leishmania mexicana

UniProt:

E9AJH1_LEIMU

TriTrypDb:

LmxM.02.0050

Length:

213

Annotations

LeishMANIAdb annotations

This cluster contains a high variety of cytochrome b5 homologs. Although all membrane-anchored, some use the N-terminal [type-III] while others the C-terminal TM helix [type-IV]. The heme domain is assumed to be cytoplasmic.. Localization: ER (by homology) / Mitochondrial outer membrane (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	13
GO:0110165	cellular anatomical entity	1	13
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2

Expansion

Sequence features

E9AJH1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AJH1

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	10
GO:0020037	heme binding	4	10
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046906	tetrapyrrole binding	3	10
GO:0097159	organic cyclic compound binding	2	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	162	164	PF00675	0.402
CLV_NRD_NRD_1	168	170	PF00675	0.521
CLV_NRD_NRD_1	66	68	PF00675	0.262
CLV_PCSK_SKI1_1	122	126	PF00082	0.241
CLV_PCSK_SKI1_1	196	200	PF00082	0.190
CLV_PCSK_SKI1_1	41	45	PF00082	0.283
DOC_MAPK_gen_1	67	74	PF00069	0.452
DOC_MAPK_MEF2A_6	196	205	PF00069	0.309
DOC_MAPK_NFAT4_5	196	204	PF00069	0.269
DOC_USP7_MATH_1	11	15	PF00917	0.386
DOC_USP7_MATH_1	93	97	PF00917	0.505
DOC_WW_Pin1_4	109	114	PF00397	0.497
LIG_14-3-3_CanoR_1	163	167	PF00244	0.689
LIG_14-3-3_CanoR_1	169	177	PF00244	0.603
LIG_14-3-3_CanoR_1	84	89	PF00244	0.612
LIG_APCC_ABBA_1	111	116	PF00400	0.487
LIG_FHA_1	110	116	PF00498	0.462
LIG_LIR_Gen_1	112	119	PF02991	0.441
LIG_LIR_Gen_1	144	155	PF02991	0.485
LIG_LIR_Gen_1	17	28	PF02991	0.523
LIG_LIR_Gen_1	98	109	PF02991	0.408
LIG_LIR_Nem_3	112	117	PF02991	0.462
LIG_LIR_Nem_3	17	23	PF02991	0.455
LIG_LIR_Nem_3	53	59	PF02991	0.441
LIG_LIR_Nem_3	98	104	PF02991	0.418
LIG_PTB_Apo_2	103	110	PF02174	0.441
LIG_PTB_Apo_2	22	29	PF02174	0.530
LIG_PTB_Phospho_1	103	109	PF10480	0.441
LIG_PTB_Phospho_1	22	28	PF10480	0.530
LIG_SH2_CRK	184	188	PF00017	0.476
LIG_SH2_NCK_1	28	32	PF00017	0.386
LIG_SH2_SRC	153	156	PF00017	0.407
LIG_SH2_SRC	34	37	PF00017	0.462
LIG_SH2_STAP1	184	188	PF00017	0.495
LIG_SH2_STAT5	153	156	PF00017	0.449
LIG_SH2_STAT5	5	8	PF00017	0.441
LIG_SH2_STAT5	85	88	PF00017	0.532
LIG_Sin3_3	200	207	PF02671	0.190
MOD_CK2_1	11	17	PF00069	0.422
MOD_GlcNHglycan	17	20	PF01048	0.287
MOD_GlcNHglycan	95	98	PF01048	0.355
MOD_GSK3_1	11	18	PF00069	0.399
MOD_GSK3_1	131	138	PF00069	0.540
MOD_GSK3_1	158	165	PF00069	0.626
MOD_GSK3_1	168	175	PF00069	0.645
MOD_N-GLC_1	59	64	PF02516	0.262
MOD_PIKK_1	131	137	PF00454	0.504
MOD_PIKK_1	172	178	PF00454	0.657
MOD_PKA_1	169	175	PF00069	0.704
MOD_PKA_2	162	168	PF00069	0.560
MOD_PKB_1	82	90	PF00069	0.584
MOD_Plk_4	162	168	PF00069	0.583
MOD_Plk_4	183	189	PF00069	0.596
MOD_ProDKin_1	109	115	PF00069	0.497
MOD_SUMO_rev_2	157	166	PF00179	0.610
TRG_DiLeu_BaEn_1	17	22	PF01217	0.437
TRG_DiLeu_BaEn_2	161	167	PF01217	0.553
TRG_ENDOCYTIC_2	147	150	PF00928	0.504
TRG_ENDOCYTIC_2	184	187	PF00928	0.478
TRG_ENDOCYTIC_2	28	31	PF00928	0.386
TRG_ENDOCYTIC_2	85	88	PF00928	0.532
TRG_ER_diArg_1	82	85	PF00400	0.540

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4I5	Leptomonas seymouri	70%	98%
A0A1X0NJR3	Trypanosomatidae	49%	96%
A0A3Q8I926	Leishmania donovani	33%	100%
A0A3S5H4X7	Leishmania donovani	83%	98%
A0A422N6Z2	Trypanosoma rangeli	48%	93%
A4H3A1	Leishmania braziliensis	72%	100%
A4H6J2	Leishmania braziliensis	30%	100%
A4HRK7	Leishmania infantum	83%	98%
A4HUW0	Leishmania infantum	31%	100%
E9AC79	Leishmania major	81%	100%
E9ANK3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
Q4QH22	Leishmania major	32%	100%