NEDD8-activating enzyme E1 catalytic subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin activating enzyme, putative

Species:

Leishmania mexicana

UniProt:

E9AJF8_LEIMU

TriTrypDb:

LmxM.01.0710

Length:

525

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AJF8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AJF8

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0019538	protein metabolic process	3	11
GO:0032446	protein modification by small protein conjugation	6	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0045116	protein neddylation	7	11
GO:0070647	protein modification by small protein conjugation or removal	5	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	11
GO:0016874	ligase activity	2	11
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	11
GO:0017076	purine nucleotide binding	4	11
GO:0019781	NEDD8 activating enzyme activity	3	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0140657	ATP-dependent activity	1	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0016740	transferase activity	2	1
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	179	185	PF00089	0.272
CLV_NRD_NRD_1	145	147	PF00675	0.303
CLV_NRD_NRD_1	149	151	PF00675	0.303
CLV_NRD_NRD_1	269	271	PF00675	0.430
CLV_NRD_NRD_1	442	444	PF00675	0.469
CLV_PCSK_FUR_1	267	271	PF00082	0.347
CLV_PCSK_KEX2_1	145	147	PF00082	0.303
CLV_PCSK_KEX2_1	267	269	PF00082	0.424
CLV_PCSK_KEX2_1	442	444	PF00082	0.469
CLV_PCSK_PC7_1	264	270	PF00082	0.447
CLV_PCSK_SKI1_1	357	361	PF00082	0.303
CLV_PCSK_SKI1_1	469	473	PF00082	0.357
CLV_PCSK_SKI1_1	84	88	PF00082	0.303
DEG_SPOP_SBC_1	164	168	PF00917	0.156
DEG_SPOP_SBC_1	96	100	PF00917	0.370
DOC_MAPK_DCC_7	334	342	PF00069	0.303
DOC_MAPK_gen_1	442	450	PF00069	0.475
DOC_MAPK_MEF2A_6	130	139	PF00069	0.287
DOC_MAPK_MEF2A_6	334	342	PF00069	0.303
DOC_MAPK_MEF2A_6	38	47	PF00069	0.253
DOC_MAPK_MEF2A_6	442	450	PF00069	0.507
DOC_PP2B_LxvP_1	430	433	PF13499	0.448
DOC_USP7_MATH_1	110	114	PF00917	0.449
DOC_USP7_MATH_1	138	142	PF00917	0.322
DOC_USP7_MATH_1	460	464	PF00917	0.364
DOC_USP7_MATH_1	510	514	PF00917	0.422
DOC_WW_Pin1_4	165	170	PF00397	0.366
DOC_WW_Pin1_4	2	7	PF00397	0.643
DOC_WW_Pin1_4	20	25	PF00397	0.394
DOC_WW_Pin1_4	213	218	PF00397	0.322
LIG_14-3-3_CanoR_1	232	236	PF00244	0.272
LIG_14-3-3_CanoR_1	443	449	PF00244	0.222
LIG_14-3-3_CanoR_1	469	476	PF00244	0.322
LIG_14-3-3_CanoR_1	511	518	PF00244	0.480
LIG_14-3-3_CanoR_1	8	18	PF00244	0.544
LIG_BIR_II_1	1	5	PF00653	0.589
LIG_BRCT_BRCA1_1	24	28	PF00533	0.447
LIG_BRCT_BRCA1_1	446	450	PF00533	0.299
LIG_BRCT_BRCA1_1	82	86	PF00533	0.257
LIG_Clathr_ClatBox_1	136	140	PF01394	0.414
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.414
LIG_FHA_1	113	119	PF00498	0.386
LIG_FHA_1	155	161	PF00498	0.378
LIG_FHA_1	214	220	PF00498	0.301
LIG_FHA_1	232	238	PF00498	0.374
LIG_FHA_1	327	333	PF00498	0.361
LIG_FHA_2	195	201	PF00498	0.279
LIG_FHA_2	380	386	PF00498	0.447
LIG_FHA_2	68	74	PF00498	0.303
LIG_FHA_2	87	93	PF00498	0.303
LIG_LIR_Gen_1	131	139	PF02991	0.426
LIG_LIR_Gen_1	39	50	PF02991	0.306
LIG_LIR_Gen_1	64	74	PF02991	0.303
LIG_LIR_Gen_1	75	82	PF02991	0.303
LIG_LIR_Nem_3	126	132	PF02991	0.303
LIG_LIR_Nem_3	30	36	PF02991	0.320
LIG_LIR_Nem_3	39	45	PF02991	0.300
LIG_LIR_Nem_3	64	69	PF02991	0.303
LIG_LIR_Nem_3	73	79	PF02991	0.303
LIG_NRBOX	56	62	PF00104	0.414
LIG_PCNA_yPIPBox_3	509	519	PF02747	0.352
LIG_PDZ_Class_2	520	525	PF00595	0.565
LIG_Pex14_2	128	132	PF04695	0.303
LIG_Pex14_2	488	492	PF04695	0.532
LIG_PTB_Apo_2	365	372	PF02174	0.376
LIG_SH2_GRB2like	381	384	PF00017	0.317
LIG_SH2_NCK_1	381	385	PF00017	0.350
LIG_SH2_PTP2	253	256	PF00017	0.447
LIG_SH2_STAT5	186	189	PF00017	0.444
LIG_SH2_STAT5	224	227	PF00017	0.414
LIG_SH2_STAT5	253	256	PF00017	0.322
LIG_SH2_STAT5	346	349	PF00017	0.414
LIG_SH2_STAT5	381	384	PF00017	0.337
LIG_SH3_1	291	297	PF00018	0.272
LIG_SH3_3	156	162	PF00018	0.421
LIG_SH3_3	241	247	PF00018	0.300
LIG_SH3_3	291	297	PF00018	0.350
LIG_SH3_3	401	407	PF00018	0.419
LIG_SH3_3	454	460	PF00018	0.548
LIG_SUMO_SIM_par_1	135	141	PF11976	0.322
LIG_SUMO_SIM_par_1	409	414	PF11976	0.324
LIG_SUMO_SIM_par_1	59	64	PF11976	0.314
LIG_TYR_ITIM	251	256	PF00017	0.447
LIG_WRC_WIRS_1	129	134	PF05994	0.370
MOD_CDC14_SPxK_1	5	8	PF00782	0.415
MOD_CDK_SPxK_1	2	8	PF00069	0.635
MOD_CK1_1	2	8	PF00069	0.669
MOD_CK1_1	416	422	PF00069	0.495
MOD_CK1_1	475	481	PF00069	0.464
MOD_CK1_1	80	86	PF00069	0.220
MOD_CK2_1	194	200	PF00069	0.279
MOD_CK2_1	379	385	PF00069	0.447
MOD_GlcNHglycan	24	27	PF01048	0.434
MOD_GlcNHglycan	394	397	PF01048	0.572
MOD_GlcNHglycan	414	418	PF01048	0.204
MOD_GlcNHglycan	451	454	PF01048	0.565
MOD_GlcNHglycan	462	465	PF01048	0.577
MOD_GlcNHglycan	512	515	PF01048	0.484
MOD_GlcNHglycan	63	66	PF01048	0.303
MOD_N-GLC_1	213	218	PF02516	0.414
MOD_N-GLC_1	80	85	PF02516	0.245
MOD_NEK2_1	61	66	PF00069	0.303
MOD_NEK2_1	86	91	PF00069	0.414
MOD_NEK2_1	9	14	PF00069	0.671
MOD_PIKK_1	268	274	PF00454	0.421
MOD_PIKK_1	9	15	PF00454	0.670
MOD_PKA_1	268	274	PF00069	0.447
MOD_PKA_2	231	237	PF00069	0.303
MOD_PKA_2	268	274	PF00069	0.449
MOD_PKA_2	510	516	PF00069	0.496
MOD_Plk_1	38	44	PF00069	0.331
MOD_Plk_1	413	419	PF00069	0.529
MOD_Plk_2-3	286	292	PF00069	0.156
MOD_Plk_4	444	450	PF00069	0.371
MOD_Plk_4	475	481	PF00069	0.487
MOD_Plk_4	67	73	PF00069	0.414
MOD_ProDKin_1	165	171	PF00069	0.366
MOD_ProDKin_1	2	8	PF00069	0.640
MOD_ProDKin_1	20	26	PF00069	0.391
MOD_ProDKin_1	213	219	PF00069	0.322
MOD_SUMO_rev_2	185	194	PF00179	0.428
TRG_DiLeu_BaEn_1	189	194	PF01217	0.447
TRG_DiLeu_BaEn_1	56	61	PF01217	0.379
TRG_ENDOCYTIC_2	129	132	PF00928	0.294
TRG_ENDOCYTIC_2	253	256	PF00928	0.303
TRG_ENDOCYTIC_2	381	384	PF00928	0.425
TRG_ER_diArg_1	145	147	PF00400	0.303
TRG_ER_diArg_1	267	270	PF00400	0.411
TRG_ER_diArg_1	441	443	PF00400	0.424
TRG_Pf-PMV_PEXEL_1	401	406	PF00026	0.442

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIU8	Leptomonas seymouri	64%	99%
A0A1X0P2L8	Trypanosomatidae	40%	100%
A0A3S7WNL1	Leishmania donovani	91%	97%
A0A422NGT4	Trypanosoma rangeli	45%	100%
A4H389	Leishmania braziliensis	81%	100%
A4HRJ4	Leishmania infantum	91%	97%
C9ZXM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9AC62	Leishmania major	88%	100%
O65041	Arabidopsis thaliana	34%	100%
Q09765	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	100%
Q5R4A0	Pongo abelii	36%	100%
Q7ZVX6	Danio rerio	36%	100%
Q8C878	Mus musculus	36%	100%
Q8TBC4	Homo sapiens	36%	100%
Q99MI7	Rattus norvegicus	36%	100%
Q9V6U8	Drosophila melanogaster	33%	100%
V5BH30	Trypanosoma cruzi	48%	100%