tRNA (adenine(58)-N(1))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA methyltransferase complex GCD14 subunit, putative

Species:

Leishmania braziliensis

UniProt:

E9AIT3_LEIBR

TriTrypDb:

LbrM.28.2600 , LBRM2903_280032700

Length:

335

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0031515	tRNA (m1A) methyltransferase complex	6	12
GO:0032991	protein-containing complex	1	12
GO:0034708	methyltransferase complex	4	12
GO:0043527	tRNA methyltransferase complex	5	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AIT3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIT3

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016426	tRNA (adenine) methyltransferase activity	6	12
GO:0016429	tRNA (adenine-N1-)-methyltransferase activity	7	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	179	183	PF00656	0.423
CLV_NRD_NRD_1	154	156	PF00675	0.202
CLV_NRD_NRD_1	305	307	PF00675	0.403
CLV_PCSK_KEX2_1	154	156	PF00082	0.209
CLV_PCSK_KEX2_1	274	276	PF00082	0.405
CLV_PCSK_KEX2_1	304	306	PF00082	0.389
CLV_PCSK_PC1ET2_1	274	276	PF00082	0.405
CLV_PCSK_PC7_1	150	156	PF00082	0.238
CLV_PCSK_SKI1_1	54	58	PF00082	0.472
DEG_Nend_Nbox_1	1	3	PF02207	0.377
DOC_MAPK_gen_1	277	287	PF00069	0.457
DOC_MAPK_MEF2A_6	10	18	PF00069	0.497
DOC_PP2B_LxvP_1	210	213	PF13499	0.312
DOC_PP4_FxxP_1	268	271	PF00568	0.388
DOC_USP7_MATH_1	201	205	PF00917	0.450
DOC_WW_Pin1_4	190	195	PF00397	0.401
DOC_WW_Pin1_4	22	27	PF00397	0.360
DOC_WW_Pin1_4	233	238	PF00397	0.289
LIG_14-3-3_CanoR_1	288	292	PF00244	0.443
LIG_BRCT_BRCA1_1	194	198	PF00533	0.197
LIG_FHA_1	121	127	PF00498	0.326
LIG_FHA_1	23	29	PF00498	0.381
LIG_FHA_1	80	86	PF00498	0.328
LIG_FHA_1	87	93	PF00498	0.313
LIG_LIR_Apic_2	266	271	PF02991	0.318
LIG_LIR_Gen_1	206	217	PF02991	0.303
LIG_LIR_Gen_1	297	307	PF02991	0.365
LIG_LIR_LC3C_4	13	18	PF02991	0.507
LIG_LIR_Nem_3	165	170	PF02991	0.324
LIG_LIR_Nem_3	206	212	PF02991	0.303
LIG_LIR_Nem_3	266	272	PF02991	0.361
LIG_LIR_Nem_3	297	302	PF02991	0.346
LIG_PDZ_Class_1	330	335	PF00595	0.757
LIG_Pex14_2	269	273	PF04695	0.466
LIG_SH2_STAP1	137	141	PF00017	0.318
LIG_SH2_STAP1	93	97	PF00017	0.303
LIG_SH2_STAT5	137	140	PF00017	0.318
LIG_SH2_STAT5	299	302	PF00017	0.341
LIG_SH2_STAT5	55	58	PF00017	0.542
LIG_SH3_1	63	69	PF00018	0.492
LIG_SH3_3	209	215	PF00018	0.312
LIG_SH3_3	63	69	PF00018	0.620
LIG_SUMO_SIM_anti_2	13	19	PF11976	0.380
LIG_SUMO_SIM_anti_2	70	75	PF11976	0.334
LIG_SUMO_SIM_anti_2	94	101	PF11976	0.302
LIG_WRC_WIRS_1	232	237	PF05994	0.318
LIG_WRC_WIRS_1	299	304	PF05994	0.319
MOD_CK1_1	193	199	PF00069	0.345
MOD_CK1_1	290	296	PF00069	0.585
MOD_CK1_1	4	10	PF00069	0.467
MOD_Cter_Amidation	108	111	PF01082	0.450
MOD_GlcNHglycan	118	121	PF01048	0.289
MOD_GlcNHglycan	296	299	PF01048	0.369
MOD_GlcNHglycan	76	79	PF01048	0.337
MOD_GSK3_1	114	121	PF00069	0.303
MOD_GSK3_1	122	129	PF00069	0.303
MOD_GSK3_1	290	297	PF00069	0.422
MOD_N-GLC_1	22	27	PF02516	0.416
MOD_N-GLC_2	133	135	PF02516	0.236
MOD_N-GLC_2	221	223	PF02516	0.423
MOD_NEK2_1	1	6	PF00069	0.532
MOD_NEK2_1	122	127	PF00069	0.318
MOD_NEK2_1	162	167	PF00069	0.388
MOD_NEK2_1	231	236	PF00069	0.318
MOD_NEK2_1	239	244	PF00069	0.318
MOD_NEK2_1	287	292	PF00069	0.560
MOD_PIKK_1	162	168	PF00454	0.423
MOD_PIKK_1	239	245	PF00454	0.303
MOD_PIKK_1	79	85	PF00454	0.308
MOD_PIKK_1	86	92	PF00454	0.295
MOD_PK_1	67	73	PF00069	0.524
MOD_PKA_2	287	293	PF00069	0.508
MOD_PKA_2	4	10	PF00069	0.407
MOD_Plk_2-3	258	264	PF00069	0.303
MOD_Plk_4	287	293	PF00069	0.490
MOD_Plk_4	67	73	PF00069	0.449
MOD_ProDKin_1	190	196	PF00069	0.397
MOD_ProDKin_1	22	28	PF00069	0.361
MOD_ProDKin_1	233	239	PF00069	0.289
MOD_SUMO_rev_2	311	319	PF00179	0.469
TRG_DiLeu_BaEn_1	68	73	PF01217	0.553
TRG_DiLeu_BaEn_4	314	320	PF01217	0.478
TRG_ENDOCYTIC_2	299	302	PF00928	0.341
TRG_ER_diArg_1	153	155	PF00400	0.202
TRG_ER_diArg_1	304	306	PF00400	0.369
TRG_NES_CRM1_1	96	107	PF08389	0.197

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PA18	Leptomonas seymouri	83%	100%
A0A0S4IUX6	Bodo saltans	59%	95%
A0A1X0NRB0	Trypanosomatidae	64%	98%
A0A3Q8IIA8	Leishmania donovani	89%	95%
A0A3R7LPM7	Trypanosoma rangeli	63%	100%
A4I3X1	Leishmania infantum	89%	95%
A6H791	Bos taurus	36%	100%
D0A938	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	80%
E9B061	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	95%
O14307	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	92%
P0CS08	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	37%	77%
P0CS09	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	37%	77%
P46959	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	35%	87%
P9WFZ0	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	30%	100%
P9WFZ1	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	30%	100%
Q4Q824	Leishmania major	88%	100%
Q4WUT7	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	29%	67%
Q57598	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	28%	100%
Q5A416	Candida albicans (strain SC5314 / ATCC MYA-2876)	35%	100%
Q6AY46	Rattus norvegicus	36%	100%
Q6BX32	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	33%	91%
Q6C0P9	Yarrowia lipolytica (strain CLIB 122 / E 150)	33%	86%
Q6CN53	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	35%	82%
Q6FL77	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	33%	90%
Q755M8	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	38%	83%
Q80XC2	Mus musculus	36%	100%
Q86JJ0	Dictyostelium discoideum	32%	100%
Q96FX7	Homo sapiens	36%	100%
Q9V1J7	Pyrococcus abyssi (strain GE5 / Orsay)	26%	100%
V5BC39	Trypanosoma cruzi	62%	100%