SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

SpoU rRNA Methylase family, putative

Species:

Leishmania braziliensis

UniProt:

E9AIR3_LEIBR

TriTrypDb:

LbrM.24.2420

Length:

465

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AIR3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIR3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0002128	tRNA nucleoside ribose methylation	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0008033	tRNA processing	8	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	10
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	10
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	10
GO:0008171	O-methyltransferase activity	5	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016300	tRNA (uracil) methyltransferase activity	6	1
GO:0016427	tRNA (cytosine) methyltransferase activity	6	1
GO:0052665	tRNA (uracil-2'-O-)-methyltransferase activity	7	1
GO:0052666	tRNA (cytosine-2'-O-)-methyltransferase activity	7	1
GO:0062105	RNA 2'-O-methyltransferase activity	5	1
GO:0106050	tRNA 2'-O-methyltransferase activity	6	1
GO:0140101	catalytic activity, acting on a tRNA	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	241	243	PF00675	0.376
CLV_NRD_NRD_1	283	285	PF00675	0.375
CLV_NRD_NRD_1	287	289	PF00675	0.415
CLV_NRD_NRD_1	335	337	PF00675	0.439
CLV_NRD_NRD_1	400	402	PF00675	0.518
CLV_PCSK_KEX2_1	241	243	PF00082	0.395
CLV_PCSK_KEX2_1	282	284	PF00082	0.410
CLV_PCSK_KEX2_1	287	289	PF00082	0.368
CLV_PCSK_KEX2_1	399	401	PF00082	0.511
CLV_PCSK_KEX2_1	7	9	PF00082	0.610
CLV_PCSK_PC1ET2_1	399	401	PF00082	0.511
CLV_PCSK_PC1ET2_1	7	9	PF00082	0.530
CLV_PCSK_PC7_1	283	289	PF00082	0.440
CLV_PCSK_SKI1_1	103	107	PF00082	0.237
CLV_PCSK_SKI1_1	235	239	PF00082	0.405
CLV_PCSK_SKI1_1	288	292	PF00082	0.490
CLV_PCSK_SKI1_1	311	315	PF00082	0.450
CLV_PCSK_SKI1_1	404	408	PF00082	0.488
CLV_PCSK_SKI1_1	459	463	PF00082	0.223
CLV_PCSK_SKI1_1	7	11	PF00082	0.496
CLV_PCSK_SKI1_1	85	89	PF00082	0.284
DEG_APCC_DBOX_1	399	407	PF00400	0.477
DEG_Nend_Nbox_1	1	3	PF02207	0.583
DOC_CDC14_PxL_1	323	331	PF14671	0.417
DOC_CYCLIN_RxL_1	308	318	PF00134	0.507
DOC_CYCLIN_yClb1_LxF_4	342	348	PF00134	0.365
DOC_CYCLIN_yClb1_LxF_4	83	88	PF00134	0.484
DOC_CYCLIN_yCln2_LP_2	321	327	PF00134	0.368
DOC_MAPK_gen_1	239	249	PF00069	0.340
DOC_MAPK_gen_1	399	410	PF00069	0.418
DOC_MAPK_gen_1	99	106	PF00069	0.452
DOC_MAPK_HePTP_8	398	410	PF00069	0.466
DOC_MAPK_MEF2A_6	130	139	PF00069	0.480
DOC_MAPK_MEF2A_6	242	251	PF00069	0.324
DOC_MAPK_MEF2A_6	401	410	PF00069	0.463
DOC_MAPK_MEF2A_6	99	106	PF00069	0.530
DOC_PP1_RVXF_1	33	39	PF00149	0.414
DOC_PP1_RVXF_1	83	89	PF00149	0.484
DOC_PP2B_LxvP_1	408	411	PF13499	0.314
DOC_USP7_MATH_1	177	181	PF00917	0.528
DOC_USP7_UBL2_3	235	239	PF12436	0.259
DOC_USP7_UBL2_3	99	103	PF12436	0.373
DOC_WW_Pin1_4	104	109	PF00397	0.443
DOC_WW_Pin1_4	209	214	PF00397	0.708
DOC_WW_Pin1_4	27	32	PF00397	0.510
DOC_WW_Pin1_4	413	418	PF00397	0.372
DOC_WW_Pin1_4	430	435	PF00397	0.460
DOC_WW_Pin1_4	71	76	PF00397	0.474
LIG_14-3-3_CanoR_1	130	138	PF00244	0.498
LIG_14-3-3_CanoR_1	201	209	PF00244	0.652
LIG_14-3-3_CanoR_1	215	221	PF00244	0.584
LIG_14-3-3_CanoR_1	241	246	PF00244	0.395
LIG_14-3-3_CanoR_1	389	398	PF00244	0.336
LIG_14-3-3_CanoR_1	8	14	PF00244	0.409
LIG_Actin_RPEL_3	234	253	PF02755	0.333
LIG_Actin_WH2_2	388	406	PF00022	0.479
LIG_Actin_WH2_2	446	461	PF00022	0.367
LIG_Actin_WH2_2	82	100	PF00022	0.373
LIG_APCC_ABBA_1	251	256	PF00400	0.334
LIG_BIR_III_4	15	19	PF00653	0.501
LIG_deltaCOP1_diTrp_1	118	127	PF00928	0.484
LIG_deltaCOP1_diTrp_1	15	23	PF00928	0.407
LIG_eIF4E_1	325	331	PF01652	0.346
LIG_FHA_1	190	196	PF00498	0.480
LIG_FHA_1	291	297	PF00498	0.349
LIG_FHA_1	31	37	PF00498	0.495
LIG_FHA_2	179	185	PF00498	0.398
LIG_FHA_2	302	308	PF00498	0.395
LIG_FHA_2	8	14	PF00498	0.488
LIG_LIR_Gen_1	275	280	PF02991	0.348
LIG_LIR_Gen_1	339	348	PF02991	0.367
LIG_LIR_Gen_1	47	58	PF02991	0.373
LIG_LIR_LC3C_4	368	373	PF02991	0.414
LIG_LIR_Nem_3	275	279	PF02991	0.425
LIG_LIR_Nem_3	318	323	PF02991	0.407
LIG_LIR_Nem_3	339	345	PF02991	0.375
LIG_LIR_Nem_3	47	53	PF02991	0.417
LIG_LIR_Nem_3	5	9	PF02991	0.407
LIG_LYPXL_yS_3	326	329	PF13949	0.344
LIG_LYPXL_yS_3	375	378	PF13949	0.393
LIG_PDZ_Class_3	460	465	PF00595	0.283
LIG_Pex14_2	2	6	PF04695	0.420
LIG_PTB_Apo_2	270	277	PF02174	0.295
LIG_PTB_Phospho_1	270	276	PF10480	0.295
LIG_REV1ctd_RIR_1	56	63	PF16727	0.517
LIG_SH2_CRK	162	166	PF00017	0.434
LIG_SH2_CRK	342	346	PF00017	0.376
LIG_SH2_PTP2	276	279	PF00017	0.356
LIG_SH2_STAP1	245	249	PF00017	0.388
LIG_SH2_STAP1	317	321	PF00017	0.373
LIG_SH2_STAT3	158	161	PF00017	0.434
LIG_SH2_STAT3	245	248	PF00017	0.340
LIG_SH2_STAT5	158	161	PF00017	0.441
LIG_SH2_STAT5	276	279	PF00017	0.367
LIG_SH2_STAT5	430	433	PF00017	0.373
LIG_SH3_3	105	111	PF00018	0.434
LIG_SH3_3	321	327	PF00018	0.426
LIG_SH3_3	370	376	PF00018	0.465
LIG_SH3_3	428	434	PF00018	0.381
LIG_SH3_3	58	64	PF00018	0.488
LIG_SUMO_SIM_anti_2	275	281	PF11976	0.385
LIG_SUMO_SIM_par_1	406	413	PF11976	0.316
LIG_TRAF2_1	115	118	PF00917	0.568
LIG_TRAF2_1	185	188	PF00917	0.400
LIG_TYR_ITIM	252	257	PF00017	0.377
LIG_TYR_ITIM	373	378	PF00017	0.409
LIG_UBA3_1	28	35	PF00899	0.421
LIG_WRC_WIRS_1	3	8	PF05994	0.414
LIG_WRC_WIRS_1	344	349	PF05994	0.402
MOD_CDC14_SPxK_1	212	215	PF00782	0.608
MOD_CDK_SPxK_1	209	215	PF00069	0.593
MOD_CK1_1	164	170	PF00069	0.453
MOD_CK1_1	190	196	PF00069	0.589
MOD_CK1_1	30	36	PF00069	0.473
MOD_CK1_1	390	396	PF00069	0.413
MOD_CK1_1	68	74	PF00069	0.544
MOD_CK2_1	178	184	PF00069	0.359
MOD_CK2_1	301	307	PF00069	0.346
MOD_Cter_Amidation	334	337	PF01082	0.563
MOD_GlcNHglycan	127	130	PF01048	0.315
MOD_GlcNHglycan	131	134	PF01048	0.323
MOD_GlcNHglycan	171	174	PF01048	0.254
MOD_GlcNHglycan	81	84	PF01048	0.353
MOD_GSK3_1	125	132	PF00069	0.461
MOD_GSK3_1	189	196	PF00069	0.567
MOD_GSK3_1	44	51	PF00069	0.373
MOD_LATS_1	63	69	PF00433	0.537
MOD_N-GLC_1	190	195	PF02516	0.557
MOD_N-GLC_1	68	73	PF02516	0.197
MOD_N-GLC_1	97	102	PF02516	0.279
MOD_NEK2_1	2	7	PF00069	0.486
MOD_NEK2_1	227	232	PF00069	0.537
MOD_NEK2_1	247	252	PF00069	0.319
MOD_NEK2_1	70	75	PF00069	0.481
MOD_NEK2_1	88	93	PF00069	0.533
MOD_NEK2_1	97	102	PF00069	0.589
MOD_PIKK_1	143	149	PF00454	0.549
MOD_PIKK_1	290	296	PF00454	0.261
MOD_PIKK_1	358	364	PF00454	0.524
MOD_PIKK_1	438	444	PF00454	0.494
MOD_PIKK_1	7	13	PF00454	0.418
MOD_PKA_1	241	247	PF00069	0.355
MOD_PKA_1	7	13	PF00069	0.379
MOD_PKA_2	129	135	PF00069	0.455
MOD_PKA_2	200	206	PF00069	0.628
MOD_PKA_2	241	247	PF00069	0.337
MOD_PKA_2	7	13	PF00069	0.441
MOD_PKB_1	457	465	PF00069	0.243
MOD_Plk_1	190	196	PF00069	0.547
MOD_Plk_1	387	393	PF00069	0.407
MOD_Plk_4	166	172	PF00069	0.405
MOD_Plk_4	247	253	PF00069	0.314
MOD_Plk_4	343	349	PF00069	0.454
MOD_ProDKin_1	104	110	PF00069	0.443
MOD_ProDKin_1	209	215	PF00069	0.711
MOD_ProDKin_1	27	33	PF00069	0.505
MOD_ProDKin_1	413	419	PF00069	0.370
MOD_ProDKin_1	430	436	PF00069	0.468
MOD_ProDKin_1	71	77	PF00069	0.474
MOD_SUMO_rev_2	41	46	PF00179	0.505
TRG_ENDOCYTIC_2	162	165	PF00928	0.437
TRG_ENDOCYTIC_2	254	257	PF00928	0.351
TRG_ENDOCYTIC_2	276	279	PF00928	0.367
TRG_ENDOCYTIC_2	326	329	PF00928	0.329
TRG_ENDOCYTIC_2	342	345	PF00928	0.377
TRG_ENDOCYTIC_2	375	378	PF00928	0.533
TRG_ER_diArg_1	282	284	PF00400	0.528
TRG_NLS_MonoCore_2	398	403	PF00514	0.485
TRG_NLS_MonoExtN_4	399	405	PF00514	0.487
TRG_Pf-PMV_PEXEL_1	311	315	PF00026	0.463

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUK1	Leptomonas seymouri	63%	99%
A0A0S4IV97	Bodo saltans	44%	92%
A0A1X0NN27	Trypanosomatidae	51%	99%
A0A3S7WYP3	Leishmania donovani	80%	99%
A0A422NV05	Trypanosoma rangeli	50%	99%
A4I142	Leishmania infantum	82%	99%
C9ZI50	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	97%
E9AX80	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	97%
Q4QA96	Leishmania major	80%	100%
V5AZL8	Trypanosoma cruzi	52%	99%