tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

E9AIR1_LEIBR

TriTrypDb:

LbrM.24.0560 , LBRM2903_240011300

Length:

437

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AIR1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIR1

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0062105	RNA 2'-O-methyltransferase activity	5	12
GO:0106050	tRNA 2'-O-methyltransferase activity	6	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	89	93	PF00656	0.452
CLV_NRD_NRD_1	224	226	PF00675	0.505
CLV_NRD_NRD_1	294	296	PF00675	0.679
CLV_NRD_NRD_1	363	365	PF00675	0.523
CLV_NRD_NRD_1	366	368	PF00675	0.474
CLV_PCSK_FUR_1	292	296	PF00082	0.657
CLV_PCSK_FUR_1	364	368	PF00082	0.340
CLV_PCSK_KEX2_1	224	226	PF00082	0.505
CLV_PCSK_KEX2_1	294	296	PF00082	0.679
CLV_PCSK_KEX2_1	363	365	PF00082	0.566
CLV_PCSK_KEX2_1	366	368	PF00082	0.517
CLV_PCSK_PC7_1	359	365	PF00082	0.625
CLV_PCSK_SKI1_1	128	132	PF00082	0.355
CLV_PCSK_SKI1_1	194	198	PF00082	0.487
CLV_PCSK_SKI1_1	224	228	PF00082	0.566
CLV_PCSK_SKI1_1	359	363	PF00082	0.604
CLV_PCSK_SKI1_1	390	394	PF00082	0.484
CLV_Separin_Metazoa	284	288	PF03568	0.589
CLV_Separin_Metazoa	405	409	PF03568	0.600
DEG_Nend_UBRbox_1	1	4	PF02207	0.474
DEG_SCF_FBW7_1	431	437	PF00400	0.451
DEG_SPOP_SBC_1	12	16	PF00917	0.407
DEG_SPOP_SBC_1	78	82	PF00917	0.673
DOC_CKS1_1	431	436	PF01111	0.460
DOC_MAPK_gen_1	189	198	PF00069	0.345
DOC_PP1_RVXF_1	374	381	PF00149	0.612
DOC_PP2B_LxvP_1	210	213	PF13499	0.504
DOC_PP2B_PxIxI_1	416	422	PF00149	0.491
DOC_PP4_FxxP_1	172	175	PF00568	0.505
DOC_USP7_MATH_1	108	112	PF00917	0.662
DOC_USP7_MATH_1	242	246	PF00917	0.539
DOC_USP7_MATH_1	265	269	PF00917	0.579
DOC_USP7_MATH_1	340	344	PF00917	0.471
DOC_WW_Pin1_4	13	18	PF00397	0.431
DOC_WW_Pin1_4	156	161	PF00397	0.505
DOC_WW_Pin1_4	235	240	PF00397	0.459
DOC_WW_Pin1_4	430	435	PF00397	0.674
DOC_WW_Pin1_4	55	60	PF00397	0.419
LIG_14-3-3_CanoR_1	144	154	PF00244	0.585
LIG_14-3-3_CanoR_1	203	213	PF00244	0.607
LIG_14-3-3_CanoR_1	267	276	PF00244	0.366
LIG_14-3-3_CanoR_1	367	373	PF00244	0.499
LIG_14-3-3_CanoR_1	379	385	PF00244	0.528
LIG_Actin_WH2_2	273	289	PF00022	0.599
LIG_BIR_III_2	382	386	PF00653	0.588
LIG_BIR_III_4	113	117	PF00653	0.435
LIG_BIR_III_4	312	316	PF00653	0.266
LIG_BRCT_BRCA1_1	301	305	PF00533	0.511
LIG_BRCT_BRCA1_1	46	50	PF00533	0.445
LIG_BRCT_BRCA1_1	79	83	PF00533	0.484
LIG_Clathr_ClatBox_1	165	169	PF01394	0.384
LIG_CSL_BTD_1	431	434	PF09270	0.452
LIG_deltaCOP1_diTrp_1	96	101	PF00928	0.327
LIG_FHA_1	183	189	PF00498	0.572
LIG_FHA_1	191	197	PF00498	0.565
LIG_FHA_1	24	30	PF00498	0.486
LIG_FHA_1	259	265	PF00498	0.481
LIG_FHA_1	268	274	PF00498	0.485
LIG_FHA_1	389	395	PF00498	0.570
LIG_FHA_2	129	135	PF00498	0.616
LIG_FHA_2	232	238	PF00498	0.457
LIG_LIR_Apic_2	169	175	PF02991	0.507
LIG_LIR_Gen_1	252	260	PF02991	0.475
LIG_LIR_Gen_1	95	105	PF02991	0.328
LIG_LIR_Nem_3	252	256	PF02991	0.510
LIG_LIR_Nem_3	258	263	PF02991	0.516
LIG_LIR_Nem_3	374	378	PF02991	0.427
LIG_LIR_Nem_3	95	101	PF02991	0.329
LIG_SH2_CRK	41	45	PF00017	0.496
LIG_SH2_STAP1	192	196	PF00017	0.467
LIG_SH2_STAP1	260	264	PF00017	0.435
LIG_SH2_STAT5	192	195	PF00017	0.445
LIG_SH2_STAT5	215	218	PF00017	0.448
LIG_SH2_STAT5	260	263	PF00017	0.435
LIG_SH3_2	385	390	PF14604	0.600
LIG_SH3_3	158	164	PF00018	0.638
LIG_SH3_3	178	184	PF00018	0.422
LIG_SH3_3	26	32	PF00018	0.482
LIG_SH3_3	331	337	PF00018	0.435
LIG_SH3_3	382	388	PF00018	0.591
LIG_SH3_3	418	424	PF00018	0.442
LIG_SH3_3	428	434	PF00018	0.612
LIG_SUMO_SIM_par_1	255	262	PF11976	0.459
LIG_TRFH_1	420	424	PF08558	0.276
LIG_UBA3_1	123	128	PF00899	0.362
LIG_WRC_WIRS_1	109	114	PF05994	0.553
LIG_WRC_WIRS_1	260	265	PF05994	0.556
LIG_WRC_WIRS_1	372	377	PF05994	0.327
MOD_CK1_1	15	21	PF00069	0.460
MOD_CK1_1	156	162	PF00069	0.430
MOD_CK1_1	23	29	PF00069	0.473
MOD_CK1_1	235	241	PF00069	0.528
MOD_CK1_1	82	88	PF00069	0.743
MOD_CK2_1	128	134	PF00069	0.382
MOD_CK2_1	231	237	PF00069	0.452
MOD_GlcNHglycan	118	121	PF01048	0.657
MOD_GlcNHglycan	17	20	PF01048	0.529
MOD_GlcNHglycan	172	175	PF01048	0.450
MOD_GlcNHglycan	22	25	PF01048	0.516
MOD_GlcNHglycan	234	237	PF01048	0.650
MOD_GlcNHglycan	400	403	PF01048	0.628
MOD_GlcNHglycan	88	91	PF01048	0.542
MOD_GlcNHglycan	98	101	PF01048	0.310
MOD_GSK3_1	11	18	PF00069	0.413
MOD_GSK3_1	118	125	PF00069	0.564
MOD_GSK3_1	204	211	PF00069	0.549
MOD_GSK3_1	228	235	PF00069	0.571
MOD_GSK3_1	351	358	PF00069	0.639
MOD_GSK3_1	40	47	PF00069	0.602
MOD_GSK3_1	430	437	PF00069	0.509
MOD_GSK3_1	78	85	PF00069	0.673
MOD_GSK3_1	92	99	PF00069	0.420
MOD_NEK2_1	118	123	PF00069	0.482
MOD_NEK2_1	154	159	PF00069	0.722
MOD_NEK2_1	196	201	PF00069	0.411
MOD_NEK2_1	259	264	PF00069	0.546
MOD_NEK2_1	368	373	PF00069	0.596
MOD_NEK2_1	398	403	PF00069	0.509
MOD_NEK2_1	83	88	PF00069	0.479
MOD_PIKK_1	118	124	PF00454	0.478
MOD_PIKK_1	145	151	PF00454	0.462
MOD_PIKK_1	64	70	PF00454	0.574
MOD_PKA_2	190	196	PF00069	0.333
MOD_PKA_2	202	208	PF00069	0.406
MOD_PKA_2	286	292	PF00069	0.605
MOD_PKA_2	378	384	PF00069	0.550
MOD_PKB_1	357	365	PF00069	0.599
MOD_Plk_4	259	265	PF00069	0.448
MOD_Plk_4	329	335	PF00069	0.429
MOD_Plk_4	40	46	PF00069	0.575
MOD_ProDKin_1	13	19	PF00069	0.436
MOD_ProDKin_1	156	162	PF00069	0.504
MOD_ProDKin_1	235	241	PF00069	0.460
MOD_ProDKin_1	430	436	PF00069	0.677
MOD_ProDKin_1	55	61	PF00069	0.432
TRG_DiLeu_BaEn_1	252	257	PF01217	0.508
TRG_DiLeu_BaLyEn_6	174	179	PF01217	0.470
TRG_DiLeu_LyEn_5	252	257	PF01217	0.508
TRG_ENDOCYTIC_2	260	263	PF00928	0.435
TRG_ENDOCYTIC_2	41	44	PF00928	0.520
TRG_ER_diArg_1	188	191	PF00400	0.343
TRG_ER_diArg_1	224	226	PF00400	0.538
TRG_ER_diArg_1	292	295	PF00400	0.649
TRG_ER_diArg_1	357	360	PF00400	0.541
TRG_ER_diArg_1	363	366	PF00400	0.539
TRG_Pf-PMV_PEXEL_1	136	141	PF00026	0.450
TRG_Pf-PMV_PEXEL_1	225	229	PF00026	0.461
TRG_Pf-PMV_PEXEL_1	408	413	PF00026	0.544

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJE0	Trypanosomatidae	31%	90%
A0A3Q8IG18	Leishmania donovani	56%	95%
A0A3S7WY71	Leishmania donovani	78%	85%
A4I0P4	Leishmania infantum	56%	95%
A4I0P5	Leishmania infantum	78%	85%
E9AWP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	58%	95%
E9AWP5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	86%
Q4QAS6	Leishmania major	78%	100%
Q4QAS7	Leishmania major	56%	100%
V5BR90	Trypanosoma cruzi	32%	82%