Putative soluble n-ethylmaleimide sensitive factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

soluble n-ethylmaleimide sensitive factor, putative

Species:

Leishmania braziliensis

UniProt:

E9AIL7_LEIBR

TriTrypDb:

LbrM.20.5850 , LBRM2903_200073700

Length:

282

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031201	SNARE complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0098796	membrane protein complex	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AIL7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AIL7

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	13
GO:0006886	intracellular protein transport	4	13
GO:0008104	protein localization	4	13
GO:0009987	cellular process	1	13
GO:0015031	protein transport	4	13
GO:0033036	macromolecule localization	2	13
GO:0045184	establishment of protein localization	3	13
GO:0046907	intracellular transport	3	13
GO:0051179	localization	1	13
GO:0051234	establishment of localization	2	13
GO:0051641	cellular localization	2	13
GO:0051649	establishment of localization in cell	3	13
GO:0070727	cellular macromolecule localization	3	13
GO:0071702	organic substance transport	4	13
GO:0071705	nitrogen compound transport	4	13
GO:0016043	cellular component organization	3	1
GO:0022411	cellular component disassembly	4	1
GO:0032984	protein-containing complex disassembly	5	1
GO:0035494	SNARE complex disassembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0000149	SNARE binding	3	1
GO:0005483	soluble NSF attachment protein activity	3	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019905	syntaxin binding	4	1
GO:0030674	protein-macromolecule adaptor activity	2	1
GO:0060090	molecular adaptor activity	1	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	121	125	PF00656	0.425
CLV_C14_Caspase3-7	2	6	PF00656	0.393
CLV_C14_Caspase3-7	211	215	PF00656	0.390
CLV_NRD_NRD_1	260	262	PF00675	0.425
CLV_PCSK_SKI1_1	107	111	PF00082	0.425
CLV_PCSK_SKI1_1	17	21	PF00082	0.312
CLV_PCSK_SKI1_1	191	195	PF00082	0.311
CLV_PCSK_SKI1_1	213	217	PF00082	0.384
CLV_PCSK_SKI1_1	86	90	PF00082	0.283
DOC_CYCLIN_RxL_1	101	114	PF00134	0.425
DOC_CYCLIN_yCln2_LP_2	177	183	PF00134	0.425
DOC_MAPK_gen_1	227	237	PF00069	0.425
DOC_MAPK_gen_1	84	93	PF00069	0.330
DOC_MAPK_MEF2A_6	230	239	PF00069	0.425
DOC_MAPK_MEF2A_6	249	256	PF00069	0.276
DOC_MAPK_MEF2A_6	267	274	PF00069	0.171
DOC_MAPK_MEF2A_6	86	95	PF00069	0.320
DOC_USP7_UBL2_3	13	17	PF12436	0.336
DOC_USP7_UBL2_3	59	63	PF12436	0.393
LIG_APCC_ABBAyCdc20_2	164	170	PF00400	0.393
LIG_BIR_II_1	1	5	PF00653	0.315
LIG_BIR_III_2	124	128	PF00653	0.425
LIG_CaM_IQ_9	254	269	PF13499	0.425
LIG_FHA_1	266	272	PF00498	0.311
LIG_FHA_2	112	118	PF00498	0.438
LIG_FHA_2	241	247	PF00498	0.405
LIG_FHA_2	85	91	PF00498	0.440
LIG_LIR_Gen_1	231	242	PF02991	0.341
LIG_LIR_Gen_1	24	35	PF02991	0.447
LIG_LIR_Nem_3	18	23	PF02991	0.398
LIG_LIR_Nem_3	231	237	PF02991	0.418
LIG_LIR_Nem_3	24	30	PF02991	0.389
LIG_PDZ_Class_1	277	282	PF00595	0.387
LIG_Pex14_2	19	23	PF04695	0.425
LIG_REV1ctd_RIR_1	17	25	PF16727	0.368
LIG_SH2_CRK	197	201	PF00017	0.447
LIG_SH2_NCK_1	169	173	PF00017	0.393
LIG_SH2_NCK_1	197	201	PF00017	0.447
LIG_SH2_NCK_1	50	54	PF00017	0.353
LIG_SH2_STAP1	129	133	PF00017	0.365
LIG_SH2_STAP1	162	166	PF00017	0.311
LIG_SH2_STAP1	169	173	PF00017	0.381
LIG_SH2_STAT5	135	138	PF00017	0.286
LIG_SH2_STAT5	188	191	PF00017	0.408
LIG_SH2_STAT5	225	228	PF00017	0.304
LIG_Sin3_3	106	113	PF02671	0.407
LIG_SUMO_SIM_anti_2	268	274	PF11976	0.336
LIG_TRAF2_1	115	118	PF00917	0.420
LIG_UBA3_1	149	154	PF00899	0.425
LIG_UBA3_1	256	262	PF00899	0.417
MOD_CK1_1	3	9	PF00069	0.367
MOD_CK2_1	111	117	PF00069	0.379
MOD_CK2_1	242	248	PF00069	0.281
MOD_GlcNHglycan	102	105	PF01048	0.464
MOD_GlcNHglycan	120	123	PF01048	0.255
MOD_GlcNHglycan	2	5	PF01048	0.327
MOD_GlcNHglycan	220	223	PF01048	0.400
MOD_PKA_2	100	106	PF00069	0.447
MOD_Plk_2-3	240	246	PF00069	0.425
MOD_Plk_4	15	21	PF00069	0.393
MOD_Plk_4	252	258	PF00069	0.311
MOD_Plk_4	3	9	PF00069	0.393
MOD_Plk_4	84	90	PF00069	0.425
MOD_SUMO_for_1	136	139	PF00179	0.194
MOD_SUMO_rev_2	139	143	PF00179	0.387
MOD_SUMO_rev_2	259	268	PF00179	0.308
MOD_SUMO_rev_2	6	15	PF00179	0.330
TRG_DiLeu_BaEn_1	233	238	PF01217	0.425
TRG_DiLeu_BaLyEn_6	104	109	PF01217	0.425
TRG_ENDOCYTIC_2	169	172	PF00928	0.327
TRG_ENDOCYTIC_2	197	200	PF00928	0.446
TRG_ENDOCYTIC_2	77	80	PF00928	0.425
TRG_Pf-PMV_PEXEL_1	107	111	PF00026	0.425
TRG_Pf-PMV_PEXEL_1	261	265	PF00026	0.311

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHW7	Leptomonas seymouri	85%	100%
A0A0N1PFE2	Leptomonas seymouri	24%	97%
A0A0S4J4X9	Bodo saltans	27%	98%
A0A0S4JSU5	Bodo saltans	51%	100%
A0A0S4JTB3	Bodo saltans	50%	100%
A0A1X0NRZ7	Trypanosomatidae	25%	97%
A0A1X0NWB5	Trypanosomatidae	57%	100%
A0A3R7P116	Trypanosoma rangeli	27%	97%
A0A3S5IRU0	Trypanosoma rangeli	54%	100%
A0A3S7WW84	Leishmania donovani	93%	100%
A0A3S7X611	Leishmania donovani	25%	97%
A4HKT1	Leishmania braziliensis	25%	100%
A4HYZ8	Leishmania infantum	93%	100%
A4I8B0	Leishmania infantum	25%	97%
C9ZIM9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
D0AAI1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	96%
E9AUV0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
E9B369	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	97%
O90758	Fowlpox virus (strain NVSL)	26%	98%
P28663	Mus musculus	28%	95%
P32602	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	97%
P54920	Homo sapiens	28%	96%
P54921	Rattus norvegicus	28%	96%
P78603	Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003)	30%	98%
P81125	Bos taurus	28%	96%
P81126	Bos taurus	28%	95%
P85969	Rattus norvegicus	28%	95%
P93798	Vitis vinifera	29%	98%
Q23983	Drosophila melanogaster	28%	97%
Q4Q4Y5	Leishmania major	25%	100%
Q4QCN3	Leishmania major	94%	100%
Q54NP6	Dictyostelium discoideum	30%	97%
Q75D68	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	28%	97%
Q9DB05	Mus musculus	28%	96%
Q9H115	Homo sapiens	28%	95%
Q9J5J0	Fowlpox virus (strain NVSL)	25%	100%
Q9M5P8	Solanum tuberosum	32%	98%
Q9P4D0	Komagataella phaffii (strain GS115 / ATCC 20864)	28%	95%
Q9P4X4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	98%
Q9P6A5	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	25%	97%
Q9SPE6	Arabidopsis thaliana	33%	98%
V5BCV5	Trypanosoma cruzi	57%	100%
V5BHZ3	Trypanosoma cruzi	25%	97%