Putative calpain-like cysteine peptidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

small myristoylated protein 1

Species:

Leishmania braziliensis

UniProt:

E9AII6_LEIBR

TriTrypDb:

LbrM.20.5530 , LBRM2903_200070200

Length:

131

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 6
Forrest at al. (procyclic)	no	yes: 8
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 32

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 15

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 30

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 63
NetGPI	no	yes: 0, no: 63

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Expansion

Sequence features

E9AII6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AII6

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	111	115	PF00656	0.452
DOC_MAPK_gen_1	110	118	PF00069	0.316
DOC_USP7_UBL2_3	28	32	PF12436	0.443
DOC_USP7_UBL2_3	72	76	PF12436	0.310
LIG_BIR_III_2	114	118	PF00653	0.472
LIG_EH1_1	56	64	PF00400	0.250
LIG_eIF4E_1	57	63	PF01652	0.250
LIG_FHA_2	53	59	PF00498	0.331
LIG_FHA_2	62	68	PF00498	0.328
LIG_FHA_2	75	81	PF00498	0.338
LIG_SH2_GRB2like	14	17	PF00017	0.516
LIG_SH2_GRB2like	50	53	PF00017	0.299
LIG_SH2_SRC	14	17	PF00017	0.505
LIG_SH2_STAP1	57	61	PF00017	0.335
LIG_SH2_STAT5	14	17	PF00017	0.503
LIG_SH3_3	89	95	PF00018	0.335
LIG_UBA3_1	36	45	PF00899	0.319
LIG_WRC_WIRS_1	62	67	PF05994	0.250
MOD_CK1_1	7	13	PF00069	0.603
MOD_CK2_1	52	58	PF00069	0.355
MOD_CK2_1	61	67	PF00069	0.381
MOD_CK2_1	74	80	PF00069	0.365
MOD_GSK3_1	3	10	PF00069	0.540
MOD_N-GLC_1	51	56	PF02516	0.384
MOD_NEK2_1	74	79	PF00069	0.360
MOD_NMyristoyl	1	7	PF02799	0.616
MOD_PIKK_1	52	58	PF00454	0.461
MOD_Plk_1	52	58	PF00069	0.419
MOD_Plk_4	10	16	PF00069	0.474
MOD_Plk_4	17	23	PF00069	0.468
MOD_Plk_4	87	93	PF00069	0.357
MOD_SPalmitoyl_4	1	7	PF01529	0.563
MOD_SUMO_rev_2	67	74	PF00179	0.374
TRG_DiLeu_BaEn_1	58	63	PF01217	0.250
TRG_DiLeu_BaEn_2	96	102	PF01217	0.379
TRG_ER_diArg_1	122	125	PF00400	0.443
TRG_NLS_MonoCore_2	42	47	PF00514	0.420
TRG_NLS_MonoExtN_4	43	48	PF00514	0.412

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	87%	99%
A0A0N0P7C3	Leptomonas seymouri	38%	90%
A0A0N1HYV4	Leptomonas seymouri	60%	100%
A0A0N1I5S6	Leptomonas seymouri	44%	85%
A0A0N1I8U4	Leptomonas seymouri	33%	97%
A0A0N1P9P7	Leptomonas seymouri	40%	67%
A0A0N1PCZ0	Leptomonas seymouri	45%	100%
A0A0N1PE11	Leptomonas seymouri	38%	92%
A0A1X0NNW3	Trypanosomatidae	50%	100%
A0A1X0NW79	Trypanosomatidae	45%	66%
A0A1X0NW95	Trypanosomatidae	78%	100%
A0A1X0NWH2	Trypanosomatidae	35%	94%
A0A1X0NWV3	Trypanosomatidae	78%	88%
A0A1X0NXS9	Trypanosomatidae	38%	96%
A0A1X0NXT9	Trypanosomatidae	39%	100%
A0A1X0NYV3	Trypanosomatidae	40%	95%
A0A3Q8I9V9	Leishmania donovani	46%	100%
A0A3Q8IBM6	Leishmania donovani	34%	98%
A0A3Q8IBP3	Leishmania donovani	43%	90%
A0A3Q8IDP8	Leishmania donovani	46%	87%
A0A3Q8IKN9	Leishmania donovani	96%	100%
A0A3R7MB04	Trypanosoma rangeli	47%	100%
A0A3R7MWC6	Trypanosoma rangeli	73%	98%
A0A3S7WW23	Leishmania donovani	55%	96%
A0A3S7WZ86	Leishmania donovani	40%	92%
A0A422MSY8	Trypanosoma rangeli	72%	78%
A0A422MSZ3	Trypanosoma rangeli	73%	100%
A0A422MUY0	Trypanosoma rangeli	45%	87%
A0A422N035	Trypanosoma rangeli	37%	94%
A0A422N058	Trypanosoma rangeli	41%	86%
A4H7N9	Leishmania braziliensis	44%	100%
A4H7P0	Leishmania braziliensis	44%	100%
A4HEG7	Leishmania braziliensis	38%	100%
A4HW29	Leishmania infantum	46%	100%
A4HYW5	Leishmania infantum	43%	90%
A4HYW6	Leishmania infantum	34%	98%
A4HYX1	Leishmania infantum	46%	87%
A4HYX3	Leishmania infantum	55%	96%
A4HYX4	Leishmania infantum	96%	100%
A4I1M3	Leishmania infantum	40%	92%
C9ZIF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
C9ZIF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E8NHG3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	78%
E9AIH7	Leishmania braziliensis	41%	90%
E9AIH8	Leishmania braziliensis	35%	95%
E9AII3	Leishmania braziliensis	46%	100%
E9AII5	Leishmania braziliensis	59%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	46%	100%
E9AUR2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	90%
E9AUR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	98%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	87%
E9AUS0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	57%	96%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	92%
P84150	Leishmania major	34%	95%
Q4Q9R4	Leishmania major	39%	100%
Q4QCS4	Leishmania major	44%	90%
Q4QFP8	Leishmania major	46%	100%
Q5SDH3	Leishmania major	41%	100%
Q5SDH4	Leishmania major	57%	100%
Q5SDH5	Leishmania major	95%	100%