Putative calpain-like cysteine peptidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania braziliensis

UniProt:

E9AII5_LEIBR

TriTrypDb:

LbrM.20.5520 , LBRM2903_200070100

Length:

136

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 5
Forrest at al. (procyclic)	no	yes: 7
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 12

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 27

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 55
NetGPI	no	yes: 0, no: 55

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Expansion

Sequence features

E9AII5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AII5

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	112	116	PF00656	0.456
CLV_PCSK_KEX2_1	8	10	PF00082	0.739
CLV_PCSK_PC1ET2_1	8	10	PF00082	0.484
CLV_PCSK_SKI1_1	127	131	PF00082	0.649
CLV_PCSK_SKI1_1	38	42	PF00082	0.418
CLV_PCSK_SKI1_1	63	67	PF00082	0.484
CLV_PCSK_SKI1_1	9	13	PF00082	0.702
DOC_PP1_RVXF_1	61	67	PF00149	0.382
DOC_USP7_MATH_1	74	78	PF00917	0.327
DOC_USP7_UBL2_3	130	134	PF12436	0.655
DOC_USP7_UBL2_3	27	31	PF12436	0.425
DOC_WW_Pin1_4	14	19	PF00397	0.298
LIG_14-3-3_CanoR_1	9	14	PF00244	0.658
LIG_APCC_ABBAyCdc20_2	98	104	PF00400	0.462
LIG_BIR_III_2	115	119	PF00653	0.527
LIG_BRCT_BRCA1_1	16	20	PF00533	0.289
LIG_deltaCOP1_diTrp_1	47	51	PF00928	0.410
LIG_FHA_1	76	82	PF00498	0.372
LIG_FHA_1	89	95	PF00498	0.291
LIG_FHA_2	66	72	PF00498	0.330
LIG_LIR_Gen_1	55	66	PF02991	0.410
LIG_LIR_Nem_3	47	52	PF02991	0.399
LIG_LIR_Nem_3	55	61	PF02991	0.402
LIG_LYPXL_yS_3	117	120	PF13949	0.444
LIG_SH2_CRK	58	62	PF00017	0.402
LIG_SUMO_SIM_anti_2	91	96	PF11976	0.421
LIG_TYR_ITSM	54	61	PF00017	0.479
LIG_UBA3_1	35	44	PF00899	0.308
MOD_CDK_SPxxK_3	14	21	PF00069	0.342
MOD_CK1_1	4	10	PF00069	0.668
MOD_CK2_1	65	71	PF00069	0.405
MOD_GlcNHglycan	122	126	PF01048	0.633
MOD_GSK3_1	70	77	PF00069	0.282
MOD_N-GLC_1	14	19	PF02516	0.391
MOD_N-GLC_1	4	9	PF02516	0.790
MOD_N-GLC_1	52	57	PF02516	0.367
MOD_NEK2_1	1	6	PF00069	0.695
MOD_NEK2_1	75	80	PF00069	0.342
MOD_NMyristoyl	1	7	PF02799	0.653
MOD_PIKK_1	53	59	PF00454	0.452
MOD_Plk_1	53	59	PF00069	0.403
MOD_Plk_1	70	76	PF00069	0.394
MOD_Plk_4	88	94	PF00069	0.357
MOD_ProDKin_1	14	20	PF00069	0.298
MOD_SUMO_rev_2	100	107	PF00179	0.486
TRG_ENDOCYTIC_2	117	120	PF00928	0.629
TRG_ENDOCYTIC_2	58	61	PF00928	0.398
TRG_NLS_MonoCore_2	40	45	PF00514	0.411
TRG_NLS_MonoExtC_3	41	47	PF00514	0.217
TRG_NLS_MonoExtN_4	38	45	PF00514	0.417

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	57%	100%
A0A0N1HYV4	Leptomonas seymouri	57%	100%
A0A0N1I5S6	Leptomonas seymouri	41%	88%
A0A0N1I8U4	Leptomonas seymouri	30%	100%
A0A0N1P9P7	Leptomonas seymouri	35%	70%
A0A0N1PCZ0	Leptomonas seymouri	51%	100%
A0A0N1PE11	Leptomonas seymouri	35%	96%
A0A1X0NNW3	Trypanosomatidae	43%	100%
A0A1X0NW79	Trypanosomatidae	42%	69%
A0A1X0NW95	Trypanosomatidae	57%	100%
A0A1X0NWV3	Trypanosomatidae	57%	91%
A0A1X0NXS9	Trypanosomatidae	35%	100%
A0A1X0NXT9	Trypanosomatidae	39%	100%
A0A1X0NYV3	Trypanosomatidae	41%	99%
A0A3Q8I9V9	Leishmania donovani	44%	100%
A0A3Q8IBM6	Leishmania donovani	30%	100%
A0A3Q8IDP8	Leishmania donovani	41%	90%
A0A3Q8IKN9	Leishmania donovani	60%	100%
A0A3R7MB04	Trypanosoma rangeli	47%	100%
A0A3R7MWC6	Trypanosoma rangeli	54%	100%
A0A3S7WW23	Leishmania donovani	78%	100%
A0A3S7WZ86	Leishmania donovani	34%	96%
A0A422MSY8	Trypanosoma rangeli	54%	81%
A0A422MSZ3	Trypanosoma rangeli	54%	100%
A0A422MUY0	Trypanosoma rangeli	34%	91%
A0A422N058	Trypanosoma rangeli	38%	89%
A4H7N9	Leishmania braziliensis	44%	100%
A4H7P0	Leishmania braziliensis	44%	100%
A4HEG7	Leishmania braziliensis	34%	100%
A4HW29	Leishmania infantum	44%	100%
A4HYW6	Leishmania infantum	30%	100%
A4HYX1	Leishmania infantum	41%	90%
A4HYX3	Leishmania infantum	78%	100%
A4HYX4	Leishmania infantum	60%	100%
A4I1M3	Leishmania infantum	34%	96%
C9ZIF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
C9ZIF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	100%
E9AIH7	Leishmania braziliensis	36%	100%
E9AIH8	Leishmania braziliensis	32%	100%
E9AII3	Leishmania braziliensis	40%	100%
E9AII6	Leishmania braziliensis	59%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	100%
E9AUR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	90%
E9AUS0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	58%	100%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	96%
P84150	Leishmania major	31%	97%
Q4Q9R4	Leishmania major	34%	100%
Q4QFP8	Leishmania major	45%	100%
Q5SDH3	Leishmania major	42%	100%
Q5SDH4	Leishmania major	76%	100%
Q5SDH5	Leishmania major	60%	100%